IE35449L - Blood analysis - Google Patents
Blood analysisInfo
- Publication number
- IE35449L IE35449L IE710890A IE89071A IE35449L IE 35449 L IE35449 L IE 35449L IE 710890 A IE710890 A IE 710890A IE 89071 A IE89071 A IE 89071A IE 35449 L IE35449 L IE 35449L
- Authority
- IE
- Ireland
- Prior art keywords
- blood
- white cells
- response
- white
- fluorescence emitted
- Prior art date
Links
- 238000004159 blood analysis Methods 0.000 title abstract 2
- 239000008280 blood Substances 0.000 abstract 3
- 210000004369 blood Anatomy 0.000 abstract 3
- 230000005855 radiation Effects 0.000 abstract 3
- DPKHZNPWBDQZCN-UHFFFAOYSA-N acridine orange free base Chemical compound C1=CC(N(C)C)=CC2=NC3=CC(N(C)C)=CC=C3C=C21 DPKHZNPWBDQZCN-UHFFFAOYSA-N 0.000 abstract 1
- 238000004458 analytical method Methods 0.000 abstract 1
- 239000007864 aqueous solution Substances 0.000 abstract 1
- DZBUGLKDJFMEHC-UHFFFAOYSA-N benzoquinolinylidene Natural products C1=CC=CC2=CC3=CC=CC=C3N=C21 DZBUGLKDJFMEHC-UHFFFAOYSA-N 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 239000002245 particle Substances 0.000 abstract 1
- 239000000243 solution Substances 0.000 abstract 1
- 239000000725 suspension Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N15/1456—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
- G01N15/1459—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
-
- G01N2015/016—
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N2015/1486—Counting the particles
Abstract
Dye composition for differential blood analysis of living white cells including an aqueous solution of acridine orange, and having a pH factor and osmolality within normal physiological ranges for human blood. The white cell analysis is made by subjecting a suspension of fresh blood in the above solution to radiation from a blue laser, distinguishing the white cells from other blood particles by detecting green fluorescence emitted from the white cells in response to the laser radiation, and measuring the magnitudes of red fluorescence emitted from individual white cells in response to the laser radiation.
[US3684377A]
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US5437870A | 1970-07-13 | 1970-07-13 |
Publications (2)
Publication Number | Publication Date |
---|---|
IE35449L true IE35449L (en) | 1972-01-13 |
IE35449B1 IE35449B1 (en) | 1976-02-18 |
Family
ID=21990637
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IE890/71A IE35449B1 (en) | 1970-07-13 | 1971-07-13 | Blood analysis method and composition |
Country Status (4)
Country | Link |
---|---|
US (1) | US3684377A (en) |
DE (1) | DE2134910C2 (en) |
GB (1) | GB1319710A (en) |
IE (1) | IE35449B1 (en) |
Families Citing this family (46)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4125828A (en) * | 1972-08-04 | 1978-11-14 | Med-El Inc. | Method and apparatus for automated classification and analysis of cells |
US3851156A (en) * | 1972-09-05 | 1974-11-26 | Green James E | Analysis method and apparatus utilizing color algebra and image processing techniques |
US3999047A (en) * | 1972-09-05 | 1976-12-21 | Green James E | Method and apparatus utilizing color algebra for analyzing scene regions |
US3883247A (en) * | 1973-10-30 | 1975-05-13 | Bio Physics Systems Inc | Method for fluorescence analysis of white blood cells |
JPS5424680B2 (en) * | 1973-11-13 | 1979-08-22 | ||
US3939350A (en) * | 1974-04-29 | 1976-02-17 | Board Of Trustees Of The Leland Stanford Junior University | Fluorescent immunoassay employing total reflection for activation |
DE2437984B2 (en) * | 1974-08-07 | 1976-06-10 | Wilhelm Will Kg, Optisches Werk, 6331 Nauborn | METHOD FOR IMPROVING THE CONTRAST OF AN OPTICAL MICROSCOPE |
US4055768A (en) * | 1976-09-07 | 1977-10-25 | Bromberg Nathan S | Light measuring apparatus |
US4343782A (en) * | 1978-04-20 | 1982-08-10 | Shapiro Howard M | Cytological assay procedure |
US4284412A (en) * | 1979-07-13 | 1981-08-18 | Ortho Diagnostics, Inc. | Method and apparatus for automated identification and enumeration of specified blood cell subclasses |
US4354114A (en) * | 1979-10-09 | 1982-10-12 | Karnaukhov Valery N | Apparatus for investigation of fluorescence characteristics of microscopic objects |
US4336029A (en) * | 1980-08-15 | 1982-06-22 | Ortho Diagnostic Systems Inc. | Method and reagents for quantitative determination of reticulocytes and platelets in whole blood |
US4390787A (en) * | 1980-10-29 | 1983-06-28 | The United States Of America As Represented By The Secretary Of Agriculture | Method and apparatus for automatic egg mass counting |
US4500509A (en) * | 1981-03-11 | 1985-02-19 | Lawrence Kass | Metachromatic dye sorption and fluorescent light emmisive means for differential determination of developmental stages of neutrophilic granulocytic cells and other leukocytes |
US4906561A (en) * | 1981-09-14 | 1990-03-06 | Thornthwaite Jerry T | Nuclear isolation medium and procedure for separating cell nuclei |
US4668618A (en) * | 1981-09-14 | 1987-05-26 | Thornthwaite Jerry T | Nuclear isolation medium and procedure for separating cell nuclei |
CA1197186A (en) * | 1982-03-19 | 1985-11-26 | Robert A. Hoffman | Method of enumerating serologically selected cell populations |
US4499052A (en) * | 1982-08-30 | 1985-02-12 | Becton, Dickinson And Company | Apparatus for distinguishing multiple subpopulations of cells |
US4559309A (en) * | 1982-09-01 | 1985-12-17 | Memorial Sloan Kettering Cancer Center | Flow cytometry-fluorescence measurements for characterizing sperm |
US4492752A (en) * | 1982-09-03 | 1985-01-08 | Ortho Diagnostics Systems Inc. | Method for discriminating between unstained and absorbing dye stained cells |
DE3238353A1 (en) * | 1982-10-15 | 1984-04-19 | Max Planck Gesellschaft zur Förderung der Wissenschaften e.V., 3400 Göttingen | METHOD FOR SIMULTANEOUSLY QUANTITATIVE DETERMINATION OF BLOOD CELLS AND REAGENT THEREFOR |
US4555396A (en) * | 1982-12-22 | 1985-11-26 | Eastman Kodak Company | Use of pyrylium and thiapyrylium compounds as biological stains |
US4840784A (en) * | 1982-12-22 | 1989-06-20 | Eastman Kodak Company | Use of pyrylium and thiapyrylium compounds as biological stains |
US4571388A (en) * | 1983-01-24 | 1986-02-18 | Becton Dickinson And Company | Detection of reticulocytes |
JPS59179073A (en) * | 1983-03-29 | 1984-10-11 | Fuji Photo Film Co Ltd | Bacteria counter |
GB2136953B (en) * | 1983-08-03 | 1985-03-20 | Wang Wei Kung | Method and apparatus detecting biological particles by induced signal |
JPH06100596B2 (en) * | 1986-09-10 | 1994-12-12 | 東亜医用電子株式会社 | Method for classifying leukocytes by flow cytometry |
CA1309327C (en) * | 1986-09-10 | 1992-10-27 | Tomoyuki Kuroda | Reagent and method for classifying leukocytes by flow cytometry |
US5175109A (en) * | 1986-09-10 | 1992-12-29 | Toa Medical Electronics Co., Ltd. | Reagent for classifying leukocytes by flow cytometry |
US5039613A (en) * | 1986-11-27 | 1991-08-13 | Toa Medical Electronics Co., Ltd. | Reagents used in a method of classifying leukocytes by flow cytometry |
US5179026A (en) * | 1986-11-27 | 1993-01-12 | Toa Medical Electronics Co., Ltd. | Method of classifying leukocytes by flow cytometry and reagents used in the method |
JP2674704B2 (en) * | 1988-06-07 | 1997-11-12 | 東亜医用電子株式会社 | Two-dimensional distribution fractionation method |
US5089384A (en) * | 1988-11-04 | 1992-02-18 | Amoco Corporation | Method and apparatus for selective cell destruction using amplified immunofluorescence |
US5075556A (en) * | 1989-12-01 | 1991-12-24 | Technicon Instruments Corporation | Acridine orange derivatives and their use in the quantitation of reticulocytes in whole blood |
JP2941041B2 (en) * | 1990-11-16 | 1999-08-25 | シスメックス株式会社 | Classification of leukocytes by flow cytometry |
US5350695A (en) * | 1991-12-05 | 1994-09-27 | Miles Inc. | Methods for the identification and characterization of reticulocytes in whole blood |
US5360739A (en) * | 1991-12-05 | 1994-11-01 | Miles Inc. | Methods for the identification and characterization of reticulocytes in whole blood |
US5284771A (en) * | 1991-12-05 | 1994-02-08 | Miles Inc. | Reagent compositions and their use in sphering cells |
US5480613A (en) * | 1992-10-22 | 1996-01-02 | Riordan; Neil H. | Apparatus kit for detecting Dientamoeba fragilis |
US5691204A (en) * | 1995-04-21 | 1997-11-25 | Abbott Laboratories | Compositions and methods for the rapid analysis of reticulocytes |
US6114173A (en) * | 1997-04-03 | 2000-09-05 | Bayer Corporation | Fully automated method and reagent composition therefor for rapid identification and characterization of reticulocytes erythrocytes and platelets in whole blood |
CN102109430B (en) * | 2009-12-25 | 2013-11-06 | 深圳迈瑞生物医疗电子股份有限公司 | Nucleated red blood cell simulation particle and blood quality control substance and preparation methods and application thereof |
WO2011123092A1 (en) * | 2010-03-29 | 2011-10-06 | Analogic Corporation | Optical detection system and/or method |
DE102013108659B3 (en) | 2013-08-09 | 2014-07-03 | Presens - Precision Sensing Gmbh | Sensor for quantitative detection of analyte in sample e.g. blood, has restriction unit which mechanically restricts change in volume of medium, so that osmolality in medium is greater than preset maximum osmolality |
DE102014107837B4 (en) | 2014-06-04 | 2021-09-02 | Presens Precision Sensing Gmbh | Optical sensor for the quantitative detection of an analyte in a sample and method for manufacturing the sensor |
DE102021134368B4 (en) | 2021-12-22 | 2023-09-21 | Hochschule Reutlingen Körperschaft des öffentlichen Rechts | Device and method for detecting marked tumor cells of a tissue in a flowing liquid |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2875666A (en) * | 1953-07-13 | 1959-03-03 | Ohio Commw Eng Co | Method of simultaneously counting red and white blood cells |
US3497690A (en) * | 1967-09-21 | 1970-02-24 | Bausch & Lomb | Method and apparatus for classifying biological cells by measuring the size and fluorescent response thereof |
-
1970
- 1970-07-13 US US54378A patent/US3684377A/en not_active Expired - Lifetime
-
1971
- 1971-07-12 GB GB3255671A patent/GB1319710A/en not_active Expired
- 1971-07-13 IE IE890/71A patent/IE35449B1/en unknown
- 1971-07-13 DE DE2134910A patent/DE2134910C2/en not_active Expired
Also Published As
Publication number | Publication date |
---|---|
DE2134910A1 (en) | 1972-01-27 |
GB1319710A (en) | 1973-06-06 |
US3684377A (en) | 1972-08-15 |
IE35449B1 (en) | 1976-02-18 |
DE2134910C2 (en) | 1982-05-13 |
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