IE35449L - Blood analysis - Google Patents

Blood analysis

Info

Publication number
IE35449L
IE35449L IE710890A IE89071A IE35449L IE 35449 L IE35449 L IE 35449L IE 710890 A IE710890 A IE 710890A IE 89071 A IE89071 A IE 89071A IE 35449 L IE35449 L IE 35449L
Authority
IE
Ireland
Prior art keywords
blood
white cells
response
white
fluorescence emitted
Prior art date
Application number
IE710890A
Other versions
IE35449B1 (en
Original Assignee
Bio Physics Systems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Physics Systems Inc filed Critical Bio Physics Systems Inc
Publication of IE35449L publication Critical patent/IE35449L/en
Publication of IE35449B1 publication Critical patent/IE35449B1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/016—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1486—Counting the particles

Landscapes

  • Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Dye composition for differential blood analysis of living white cells including an aqueous solution of acridine orange, and having a pH factor and osmolality within normal physiological ranges for human blood. The white cell analysis is made by subjecting a suspension of fresh blood in the above solution to radiation from a blue laser, distinguishing the white cells from other blood particles by detecting green fluorescence emitted from the white cells in response to the laser radiation, and measuring the magnitudes of red fluorescence emitted from individual white cells in response to the laser radiation. [US3684377A]
IE890/71A 1970-07-13 1971-07-13 Blood analysis method and composition IE35449B1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US5437870A 1970-07-13 1970-07-13

Publications (2)

Publication Number Publication Date
IE35449L true IE35449L (en) 1972-01-13
IE35449B1 IE35449B1 (en) 1976-02-18

Family

ID=21990637

Family Applications (1)

Application Number Title Priority Date Filing Date
IE890/71A IE35449B1 (en) 1970-07-13 1971-07-13 Blood analysis method and composition

Country Status (4)

Country Link
US (1) US3684377A (en)
DE (1) DE2134910C2 (en)
GB (1) GB1319710A (en)
IE (1) IE35449B1 (en)

Families Citing this family (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4125828A (en) * 1972-08-04 1978-11-14 Med-El Inc. Method and apparatus for automated classification and analysis of cells
US3851156A (en) * 1972-09-05 1974-11-26 Green James E Analysis method and apparatus utilizing color algebra and image processing techniques
US3999047A (en) * 1972-09-05 1976-12-21 Green James E Method and apparatus utilizing color algebra for analyzing scene regions
US3883247A (en) * 1973-10-30 1975-05-13 Bio Physics Systems Inc Method for fluorescence analysis of white blood cells
JPS5424680B2 (en) * 1973-11-13 1979-08-22
US3939350A (en) * 1974-04-29 1976-02-17 Board Of Trustees Of The Leland Stanford Junior University Fluorescent immunoassay employing total reflection for activation
DE2437984B2 (en) * 1974-08-07 1976-06-10 Wilhelm Will Kg, Optisches Werk, 6331 Nauborn METHOD FOR IMPROVING THE CONTRAST OF AN OPTICAL MICROSCOPE
US4055768A (en) * 1976-09-07 1977-10-25 Bromberg Nathan S Light measuring apparatus
US4343782A (en) * 1978-04-20 1982-08-10 Shapiro Howard M Cytological assay procedure
US4284412A (en) * 1979-07-13 1981-08-18 Ortho Diagnostics, Inc. Method and apparatus for automated identification and enumeration of specified blood cell subclasses
US4354114A (en) * 1979-10-09 1982-10-12 Karnaukhov Valery N Apparatus for investigation of fluorescence characteristics of microscopic objects
US4336029A (en) * 1980-08-15 1982-06-22 Ortho Diagnostic Systems Inc. Method and reagents for quantitative determination of reticulocytes and platelets in whole blood
US4390787A (en) * 1980-10-29 1983-06-28 The United States Of America As Represented By The Secretary Of Agriculture Method and apparatus for automatic egg mass counting
US4500509A (en) * 1981-03-11 1985-02-19 Lawrence Kass Metachromatic dye sorption and fluorescent light emmisive means for differential determination of developmental stages of neutrophilic granulocytic cells and other leukocytes
US4668618A (en) * 1981-09-14 1987-05-26 Thornthwaite Jerry T Nuclear isolation medium and procedure for separating cell nuclei
US4906561A (en) * 1981-09-14 1990-03-06 Thornthwaite Jerry T Nuclear isolation medium and procedure for separating cell nuclei
CA1197186A (en) * 1982-03-19 1985-11-26 Robert A. Hoffman Method of enumerating serologically selected cell populations
US4499052A (en) * 1982-08-30 1985-02-12 Becton, Dickinson And Company Apparatus for distinguishing multiple subpopulations of cells
US4559309A (en) * 1982-09-01 1985-12-17 Memorial Sloan Kettering Cancer Center Flow cytometry-fluorescence measurements for characterizing sperm
US4492752A (en) * 1982-09-03 1985-01-08 Ortho Diagnostics Systems Inc. Method for discriminating between unstained and absorbing dye stained cells
DE3238353A1 (en) * 1982-10-15 1984-04-19 Max Planck Gesellschaft zur Förderung der Wissenschaften e.V., 3400 Göttingen METHOD FOR SIMULTANEOUSLY QUANTITATIVE DETERMINATION OF BLOOD CELLS AND REAGENT THEREFOR
US4555396A (en) * 1982-12-22 1985-11-26 Eastman Kodak Company Use of pyrylium and thiapyrylium compounds as biological stains
US4840784A (en) * 1982-12-22 1989-06-20 Eastman Kodak Company Use of pyrylium and thiapyrylium compounds as biological stains
US4571388A (en) * 1983-01-24 1986-02-18 Becton Dickinson And Company Detection of reticulocytes
JPS59179073A (en) * 1983-03-29 1984-10-11 Fuji Photo Film Co Ltd Bacteria counter
GB2136953B (en) * 1983-08-03 1985-03-20 Wang Wei Kung Method and apparatus detecting biological particles by induced signal
US5175109A (en) * 1986-09-10 1992-12-29 Toa Medical Electronics Co., Ltd. Reagent for classifying leukocytes by flow cytometry
JPH06100596B2 (en) * 1986-09-10 1994-12-12 東亜医用電子株式会社 Method for classifying leukocytes by flow cytometry
CA1309327C (en) * 1986-09-10 1992-10-27 Tomoyuki Kuroda Reagent and method for classifying leukocytes by flow cytometry
US5179026A (en) * 1986-11-27 1993-01-12 Toa Medical Electronics Co., Ltd. Method of classifying leukocytes by flow cytometry and reagents used in the method
US5039613A (en) * 1986-11-27 1991-08-13 Toa Medical Electronics Co., Ltd. Reagents used in a method of classifying leukocytes by flow cytometry
JP2674704B2 (en) * 1988-06-07 1997-11-12 東亜医用電子株式会社 Two-dimensional distribution fractionation method
US5089384A (en) * 1988-11-04 1992-02-18 Amoco Corporation Method and apparatus for selective cell destruction using amplified immunofluorescence
US5075556A (en) * 1989-12-01 1991-12-24 Technicon Instruments Corporation Acridine orange derivatives and their use in the quantitation of reticulocytes in whole blood
JP2941041B2 (en) * 1990-11-16 1999-08-25 シスメックス株式会社 Classification of leukocytes by flow cytometry
US5284771A (en) * 1991-12-05 1994-02-08 Miles Inc. Reagent compositions and their use in sphering cells
US5350695A (en) * 1991-12-05 1994-09-27 Miles Inc. Methods for the identification and characterization of reticulocytes in whole blood
US5360739A (en) * 1991-12-05 1994-11-01 Miles Inc. Methods for the identification and characterization of reticulocytes in whole blood
WO1995030767A1 (en) * 1992-10-22 1995-11-16 The Center For The Improvement Of Human Functioning International, Inc. METHOD FOR DETECTING INTESTINAL PATHOGEN $i(DIENTAMOEBA FRAGILIS)
US5691204A (en) * 1995-04-21 1997-11-25 Abbott Laboratories Compositions and methods for the rapid analysis of reticulocytes
US6114173A (en) * 1997-04-03 2000-09-05 Bayer Corporation Fully automated method and reagent composition therefor for rapid identification and characterization of reticulocytes erythrocytes and platelets in whole blood
CN102109430B (en) * 2009-12-25 2013-11-06 深圳迈瑞生物医疗电子股份有限公司 Nucleated red blood cell simulation particle and blood quality control substance and preparation methods and application thereof
US20130011928A1 (en) * 2010-03-29 2013-01-10 Analogic Corporation Optical detection system and/or method
DE102013108659B3 (en) 2013-08-09 2014-07-03 Presens - Precision Sensing Gmbh Sensor for quantitative detection of analyte in sample e.g. blood, has restriction unit which mechanically restricts change in volume of medium, so that osmolality in medium is greater than preset maximum osmolality
DE102014107837B4 (en) 2014-06-04 2021-09-02 Presens Precision Sensing Gmbh Optical sensor for the quantitative detection of an analyte in a sample and method for manufacturing the sensor
DE102021134368B4 (en) 2021-12-22 2023-09-21 Hochschule Reutlingen Körperschaft des öffentlichen Rechts Device and method for detecting marked tumor cells of a tissue in a flowing liquid
WO2024194666A1 (en) 2023-03-21 2024-09-26 Bit Group France Method of fluorescent dye variation compensation by free state fluorescence measurement

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2875666A (en) * 1953-07-13 1959-03-03 Ohio Commw Eng Co Method of simultaneously counting red and white blood cells
US3497690A (en) * 1967-09-21 1970-02-24 Bausch & Lomb Method and apparatus for classifying biological cells by measuring the size and fluorescent response thereof

Also Published As

Publication number Publication date
DE2134910A1 (en) 1972-01-27
US3684377A (en) 1972-08-15
IE35449B1 (en) 1976-02-18
DE2134910C2 (en) 1982-05-13
GB1319710A (en) 1973-06-06

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