GB1377346A - Method of and reagent for testing blood - Google Patents

Method of and reagent for testing blood

Info

Publication number
GB1377346A
GB1377346A GB1444373A GB1444373A GB1377346A GB 1377346 A GB1377346 A GB 1377346A GB 1444373 A GB1444373 A GB 1444373A GB 1444373 A GB1444373 A GB 1444373A GB 1377346 A GB1377346 A GB 1377346A
Authority
GB
United Kingdom
Prior art keywords
solution
hemoglobin
sickle
grams
indicates
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB1444373A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Baxter International Inc
Original Assignee
Baxter Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Baxter Laboratories Inc filed Critical Baxter Laboratories Inc
Publication of GB1377346A publication Critical patent/GB1377346A/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
    • G01N33/721—Haemoglobin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Physiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

1377346 Diagnostic agent for detecting sicklecell disease BAXTER LABORATORIES Inc 26 March 1973 [24 March 1972] 14443/73 Heading G1B A reagent kit for use in testing blood for sicklecell disease and for distinguishing between sicklecell anemia and sickle-cell trait comprises (a) an aqueous buffer solution having a pH of from 7 to 7À5 and containing, per 100 ml of solution, from 21 to 35 grams of (NH 4 ) 2 SO 4 and from 0À5 to 2À0 grams of K 2 HPO 4 , (b) a hemolyzing agent, for example saponin, and (c) a reducing agent, for example sodium dithionite. The hemolyzing agent and the reducing agent may be in powdered form. Reagent solution prepared by mixing the contents of such kit may contain, per 100 ml of solution, from 0À01 to 2 grams of hemolyzing agent and from 0À01 to 2 grams of reducing agent. A sample of unclotted blood is mixed with a predetermined amount of reagent solution, the mixture being observed visually both before and after centrifuging. A translucent solution before centrifuging indicates normal (AA) hemoglobin whereas a turbid solution indicates either sickle-cell trait (AS) hemoglobin or sickle-cell anemia (SS) hemoglobin; a small amount of white precipitate after centrifuging indicates normal hemoglobin whereas a pink solution with red precipitate indicates sickle-cell hemoglobin and a yellow solution with red precipitate indicates sickle-cell anemia hemoglobin. The buffer solution additionally may contain a redox dye, for example methylene blue or basic fuchsin, for indicating decomposition of sodium dithionite reducing agent.
GB1444373A 1972-03-24 1973-03-26 Method of and reagent for testing blood Expired GB1377346A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US23792472A 1972-03-24 1972-03-24

Publications (1)

Publication Number Publication Date
GB1377346A true GB1377346A (en) 1974-12-11

Family

ID=22895800

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1444373A Expired GB1377346A (en) 1972-03-24 1973-03-26 Method of and reagent for testing blood

Country Status (4)

Country Link
US (1) US3761226A (en)
CA (1) CA1009574A (en)
GB (1) GB1377346A (en)
ZA (1) ZA731900B (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3918905A (en) * 1973-07-20 1975-11-11 Biolog Corp Of America Diagnostic test for the determination of sickling hemoglobinopathies
US4236526A (en) * 1978-01-31 1980-12-02 Richard Patricia A Method of screening for sickle cell disease by detection of porphyrins and porphyrin metabolites in human dentition
US5514592A (en) * 1995-02-13 1996-05-07 Medicus Technologies, Inc. Method and composition for testing blood for hemoglobin S that incorporates mineral oil as an insoluble upper phase
US8623659B2 (en) 2010-09-24 2014-01-07 Saint Louis University Sickle confirm modified hemoglobin solubility test
US9448230B2 (en) 2012-11-21 2016-09-20 Saint Louis Univeristy Method of hemoglobin-F determination

Also Published As

Publication number Publication date
CA1009574A (en) 1977-05-03
US3761226A (en) 1973-09-25
ZA731900B (en) 1973-12-19

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Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PCNP Patent ceased through non-payment of renewal fee