GB1319710A - Blood analysis method and composition - Google Patents

Blood analysis method and composition

Info

Publication number
GB1319710A
GB1319710A GB3255671A GB3255671A GB1319710A GB 1319710 A GB1319710 A GB 1319710A GB 3255671 A GB3255671 A GB 3255671A GB 3255671 A GB3255671 A GB 3255671A GB 1319710 A GB1319710 A GB 1319710A
Authority
GB
United Kingdom
Prior art keywords
cells
dye
solution
white
july
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB3255671A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BIO PHYSICS SYSTEMS Inc
Original Assignee
BIO PHYSICS SYSTEMS Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BIO PHYSICS SYSTEMS Inc filed Critical BIO PHYSICS SYSTEMS Inc
Publication of GB1319710A publication Critical patent/GB1319710A/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1456—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G01N2015/016—
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N2015/1486—Counting the particles

Abstract

1319710 Dye composition for distinguishing blood cells BIO/PHYSICS SYSTEMS INC 12 July 1971 [13 July 1970] 32556/71 Heading B1X [Also in Divisions C4 G1 and G4] In the analysis of blood samples in order to distinguish white from other cells, the samples are combined with a fluorescent dye which is absorbed mainly by the white cells. The dye may be acridine orange (3, 6-bisdimethyl-amino-acridinium-choride) in aqueous solution in a concentration between 10<SP>-7</SP> and 10<SP>-5</SP> gms per cc., and having a pH factor and an osmolality within the normal physiological ranges for human blood plasma. The pH factor may be obtained by addition of phosphates e.g. NaH 2 PO 4 and Na 2 HPO 4 and the osmolality adjusted by addition of sodium chloride e.g. to give a 0.85% solution. A method of producing a solution such as the latter is described. Instead of the phosphate buffer, HEPES (N-2-hydroxyethylpiperazine- N'-2-ethanesulfonic acid), or TRIS (2-amino- 2-(hydroxy-methyl)-1, 3-propanediol), may be used. Examples of various concentrations of acridine orange solution were given. The dye is characterised by a green fluorescence for white cells and red for granulocytic cells. Other dyes are mentioned which produce a fluorescent effect with cells:-phosphine (very mild-yellow for white cells) and (even more mild fluorescence) phenosafranine, neutral red, auromine O and acradine yellow.
GB3255671A 1970-07-13 1971-07-12 Blood analysis method and composition Expired GB1319710A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US5437870A 1970-07-13 1970-07-13

Publications (1)

Publication Number Publication Date
GB1319710A true GB1319710A (en) 1973-06-06

Family

ID=21990637

Family Applications (1)

Application Number Title Priority Date Filing Date
GB3255671A Expired GB1319710A (en) 1970-07-13 1971-07-12 Blood analysis method and composition

Country Status (4)

Country Link
US (1) US3684377A (en)
DE (1) DE2134910C2 (en)
GB (1) GB1319710A (en)
IE (1) IE35449B1 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0005032A2 (en) * 1978-04-20 1979-10-31 Howard Maurice Shapiro A bioassay method
GB2116712A (en) * 1982-03-09 1983-09-28 Lawrence Kass Metachromatic dye sorption and fluorescent light emissive means for differential determination of developmental stages of neutrophilic granulocytic cells and other leukocytes
GB2136953A (en) * 1983-08-03 1984-09-26 Wang Wei Kung Method and Apparatus for Detecting Biological Particles by Induced Signal

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US4125828A (en) * 1972-08-04 1978-11-14 Med-El Inc. Method and apparatus for automated classification and analysis of cells
US3999047A (en) * 1972-09-05 1976-12-21 Green James E Method and apparatus utilizing color algebra for analyzing scene regions
US3851156A (en) * 1972-09-05 1974-11-26 Green James E Analysis method and apparatus utilizing color algebra and image processing techniques
US3883247A (en) * 1973-10-30 1975-05-13 Bio Physics Systems Inc Method for fluorescence analysis of white blood cells
JPS5424680B2 (en) * 1973-11-13 1979-08-22
US3939350A (en) * 1974-04-29 1976-02-17 Board Of Trustees Of The Leland Stanford Junior University Fluorescent immunoassay employing total reflection for activation
DE2437984B2 (en) * 1974-08-07 1976-06-10 Wilhelm Will Kg, Optisches Werk, 6331 Nauborn METHOD FOR IMPROVING THE CONTRAST OF AN OPTICAL MICROSCOPE
US4055768A (en) * 1976-09-07 1977-10-25 Bromberg Nathan S Light measuring apparatus
US4284412A (en) * 1979-07-13 1981-08-18 Ortho Diagnostics, Inc. Method and apparatus for automated identification and enumeration of specified blood cell subclasses
US4354114A (en) * 1979-10-09 1982-10-12 Karnaukhov Valery N Apparatus for investigation of fluorescence characteristics of microscopic objects
US4336029A (en) * 1980-08-15 1982-06-22 Ortho Diagnostic Systems Inc. Method and reagents for quantitative determination of reticulocytes and platelets in whole blood
US4390787A (en) * 1980-10-29 1983-06-28 The United States Of America As Represented By The Secretary Of Agriculture Method and apparatus for automatic egg mass counting
US4906561A (en) * 1981-09-14 1990-03-06 Thornthwaite Jerry T Nuclear isolation medium and procedure for separating cell nuclei
US4668618A (en) * 1981-09-14 1987-05-26 Thornthwaite Jerry T Nuclear isolation medium and procedure for separating cell nuclei
CA1197186A (en) * 1982-03-19 1985-11-26 Robert A. Hoffman Method of enumerating serologically selected cell populations
US4499052A (en) * 1982-08-30 1985-02-12 Becton, Dickinson And Company Apparatus for distinguishing multiple subpopulations of cells
US4559309A (en) * 1982-09-01 1985-12-17 Memorial Sloan Kettering Cancer Center Flow cytometry-fluorescence measurements for characterizing sperm
US4492752A (en) * 1982-09-03 1985-01-08 Ortho Diagnostics Systems Inc. Method for discriminating between unstained and absorbing dye stained cells
DE3238353A1 (en) * 1982-10-15 1984-04-19 Max Planck Gesellschaft zur Förderung der Wissenschaften e.V., 3400 Göttingen METHOD FOR SIMULTANEOUSLY QUANTITATIVE DETERMINATION OF BLOOD CELLS AND REAGENT THEREFOR
US4555396A (en) * 1982-12-22 1985-11-26 Eastman Kodak Company Use of pyrylium and thiapyrylium compounds as biological stains
US4840784A (en) * 1982-12-22 1989-06-20 Eastman Kodak Company Use of pyrylium and thiapyrylium compounds as biological stains
US4571388A (en) * 1983-01-24 1986-02-18 Becton Dickinson And Company Detection of reticulocytes
JPS59179073A (en) * 1983-03-29 1984-10-11 Fuji Photo Film Co Ltd Bacteria counter
CA1309327C (en) * 1986-09-10 1992-10-27 Tomoyuki Kuroda Reagent and method for classifying leukocytes by flow cytometry
JPH06100596B2 (en) * 1986-09-10 1994-12-12 東亜医用電子株式会社 Method for classifying leukocytes by flow cytometry
US5175109A (en) * 1986-09-10 1992-12-29 Toa Medical Electronics Co., Ltd. Reagent for classifying leukocytes by flow cytometry
US5039613A (en) * 1986-11-27 1991-08-13 Toa Medical Electronics Co., Ltd. Reagents used in a method of classifying leukocytes by flow cytometry
US5179026A (en) * 1986-11-27 1993-01-12 Toa Medical Electronics Co., Ltd. Method of classifying leukocytes by flow cytometry and reagents used in the method
JP2674704B2 (en) * 1988-06-07 1997-11-12 東亜医用電子株式会社 Two-dimensional distribution fractionation method
US5089384A (en) * 1988-11-04 1992-02-18 Amoco Corporation Method and apparatus for selective cell destruction using amplified immunofluorescence
US5075556A (en) * 1989-12-01 1991-12-24 Technicon Instruments Corporation Acridine orange derivatives and their use in the quantitation of reticulocytes in whole blood
JP2941041B2 (en) * 1990-11-16 1999-08-25 シスメックス株式会社 Classification of leukocytes by flow cytometry
US5360739A (en) * 1991-12-05 1994-11-01 Miles Inc. Methods for the identification and characterization of reticulocytes in whole blood
US5350695A (en) * 1991-12-05 1994-09-27 Miles Inc. Methods for the identification and characterization of reticulocytes in whole blood
US5284771A (en) * 1991-12-05 1994-02-08 Miles Inc. Reagent compositions and their use in sphering cells
WO1995030767A1 (en) * 1992-10-22 1995-11-16 The Center For The Improvement Of Human Functioning International, Inc. METHOD FOR DETECTING INTESTINAL PATHOGEN $i(DIENTAMOEBA FRAGILIS)
US5691204A (en) * 1995-04-21 1997-11-25 Abbott Laboratories Compositions and methods for the rapid analysis of reticulocytes
US6114173A (en) * 1997-04-03 2000-09-05 Bayer Corporation Fully automated method and reagent composition therefor for rapid identification and characterization of reticulocytes erythrocytes and platelets in whole blood
CN102109430B (en) * 2009-12-25 2013-11-06 深圳迈瑞生物医疗电子股份有限公司 Nucleated red blood cell simulation particle and blood quality control substance and preparation methods and application thereof
EP2553454A1 (en) * 2010-03-29 2013-02-06 Analogic Corporation Optical detection system and/or method
DE102013108659B3 (en) 2013-08-09 2014-07-03 Presens - Precision Sensing Gmbh Sensor for quantitative detection of analyte in sample e.g. blood, has restriction unit which mechanically restricts change in volume of medium, so that osmolality in medium is greater than preset maximum osmolality
DE102014107837B4 (en) 2014-06-04 2021-09-02 Presens Precision Sensing Gmbh Optical sensor for the quantitative detection of an analyte in a sample and method for manufacturing the sensor
DE102021134368B4 (en) 2021-12-22 2023-09-21 Hochschule Reutlingen Körperschaft des öffentlichen Rechts Device and method for detecting marked tumor cells of a tissue in a flowing liquid

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2875666A (en) * 1953-07-13 1959-03-03 Ohio Commw Eng Co Method of simultaneously counting red and white blood cells
US3497690A (en) * 1967-09-21 1970-02-24 Bausch & Lomb Method and apparatus for classifying biological cells by measuring the size and fluorescent response thereof

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0005032A2 (en) * 1978-04-20 1979-10-31 Howard Maurice Shapiro A bioassay method
EP0005032A3 (en) * 1978-04-20 1980-04-16 Howard Maurice Shapiro A bioassay method
US4343782A (en) * 1978-04-20 1982-08-10 Shapiro Howard M Cytological assay procedure
GB2116712A (en) * 1982-03-09 1983-09-28 Lawrence Kass Metachromatic dye sorption and fluorescent light emissive means for differential determination of developmental stages of neutrophilic granulocytic cells and other leukocytes
GB2136953A (en) * 1983-08-03 1984-09-26 Wang Wei Kung Method and Apparatus for Detecting Biological Particles by Induced Signal

Also Published As

Publication number Publication date
DE2134910A1 (en) 1972-01-27
DE2134910C2 (en) 1982-05-13
IE35449B1 (en) 1976-02-18
IE35449L (en) 1972-01-13
US3684377A (en) 1972-08-15

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Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PE20 Patent expired after termination of 20 years