HUP9900057A2 - Dna constructs - Google Patents

Dna constructs

Info

Publication number
HUP9900057A2
HUP9900057A2 HU9900057A HUP9900057A HUP9900057A2 HU P9900057 A2 HUP9900057 A2 HU P9900057A2 HU 9900057 A HU9900057 A HU 9900057A HU P9900057 A HUP9900057 A HU P9900057A HU P9900057 A2 HUP9900057 A2 HU P9900057A2
Authority
HU
Hungary
Prior art keywords
protein
inducible
expression
spores
gene
Prior art date
Application number
HU9900057A
Other languages
Hungarian (hu)
Inventor
Ian Jepson
Jacqueline Ann Mary Paine
Original Assignee
Zeneca Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zeneca Limited filed Critical Zeneca Limited
Publication of HUP9900057A2 publication Critical patent/HUP9900057A2/en
Publication of HUP9900057A3 publication Critical patent/HUP9900057A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Insects & Arthropods (AREA)
  • Pest Control & Pesticides (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Peptides Or Proteins (AREA)

Abstract

A találmány tárgya kémiailag indűkálható növényi génkifejező kazetta,amely egy alcR génből származó és regűlátőr fehérjét kódőlóregűlátőrszekvenciáhőz működőképesen kapcsőlt első prőmőtőrttartalmaz, tartalmaz tővább, egy indűkálható prőmőtőrt működőképesenegy őlyan célgénhez kapcsőlva, amely rővarőkat kárősító fehérjét kódőlvagy amelynek kifejeződése rővarőkat kárősító anyagcseretermékképződéséhez vezető anyagcsere-főlyamatőt indít be, amely indűkálhatóprőmőtőrt a regűlátőr fehérje aktiválja egy hatásős külső indűkálószerjelenlétében őly módőn, hőgy az indűkálószer felvitele a célgénkifejeződését eredményezi. ŕThe subject of the invention is a chemically inducible plant gene expression cassette, which contains a first primer from an alcR gene and operably fused to a regulatory sequence encoding a regulatory protein, further containing an inducible primer operatively fused to a target gene that codes for a protein that damages the spores or the expression of which is responsible for the damage to the spores initiates a metabolic supercharger leading to the formation of metabolic products , which is an inducible protein, is activated by the regulatory protein in the presence of an effective external inducer in such a way that the application of the inducer results in the expression of the target gene. ŕ

HU9900057A 1995-08-08 1996-07-29 Dna constructs HUP9900057A3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB9516241.8A GB9516241D0 (en) 1995-08-08 1995-08-08 Dna constructs

Publications (2)

Publication Number Publication Date
HUP9900057A2 true HUP9900057A2 (en) 1999-04-28
HUP9900057A3 HUP9900057A3 (en) 2001-06-28

Family

ID=10778942

Family Applications (1)

Application Number Title Priority Date Filing Date
HU9900057A HUP9900057A3 (en) 1995-08-08 1996-07-29 Dna constructs

Country Status (19)

Country Link
EP (1) EP0846179A2 (en)
JP (1) JPH11510694A (en)
KR (1) KR19990036251A (en)
CN (1) CN1199425A (en)
AP (1) AP863A (en)
AR (1) AR002914A1 (en)
AU (1) AU704172B2 (en)
BR (1) BR9609873A (en)
CA (1) CA2227445A1 (en)
CZ (1) CZ36998A3 (en)
GB (1) GB9516241D0 (en)
HU (1) HUP9900057A3 (en)
IL (1) IL123172A0 (en)
MX (1) MX9801008A (en)
NZ (1) NZ313724A (en)
PL (1) PL324880A1 (en)
SK (1) SK16998A3 (en)
TR (1) TR199800177T1 (en)
WO (1) WO1997006268A2 (en)

Families Citing this family (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9606062D0 (en) 1996-03-22 1996-05-22 Zeneca Ltd Promoters
GB9817704D0 (en) * 1998-08-13 1998-10-07 Zeneca Ltd Gene switch
GB9902234D0 (en) * 1999-02-01 1999-03-24 Zeneca Ltd Expression system
GB9902236D0 (en) * 1999-02-01 1999-03-24 Zeneca Ltd Formulation
GB9918154D0 (en) * 1999-08-02 1999-10-06 Zeneca Ltd Expression system
EP1925672A1 (en) 2001-06-22 2008-05-28 Syngeta Participations AG Abiotic stress responsive polynucleotides and polypeptides
DE10212158A1 (en) 2002-03-19 2003-10-02 Metanomics Gmbh & Co Kgaa Population of transgenic plants, derived biological material, corresponding plasmid collection and population of transformed host organisms, as well as their use and methods for their production
AU2003294924A1 (en) 2002-12-20 2004-07-14 Metanomics Gmbh & Co. Kgaa Method for producing aminoacids
BRPI0407514A (en) 2003-02-17 2006-02-14 Metanomics Gmbh method for preparing a non-human organism, polynucleotide, method for preparing a vector, vector, host cell, polypeptide, antibody, antisense polynucleic acid, method for preparing a plant, plant cell, transgenic plant tissue, useful animal cell , useful animal or a transgenic microorganism non-human organism, transgenic microorganism, yield or a propagating material of a useful plant or animal, biomass of a microorganism, method for preparing fine chemicals, and, use of polypeptide
WO2005014828A2 (en) 2003-08-01 2005-02-17 Basf Plant Science Gmbh Process for the production of fine chemicals in plants
EP2080769A3 (en) 2004-07-02 2010-12-01 Metanomics GmbH Process for the production of fine chemicals
BRPI0514003A (en) 2004-07-31 2008-05-20 Metanomics Gmbh methods for preparing a non-human organism, preparing a vector, a transgenic plant, a plant cell, a plant tissue, a useful animal's cell, a useful animal or a transgenic microorganism, and fine chemicals, and for identifying a gene product, polynucleotide , vector, host cell, antibody, antisense nucleic acid, non-human organism, transgenic microorganism, seed, tuber or propagation material of a plant, biomass of a microorganism, plant cell, plant cell organelle, plant tissue, plant or part thereof , and, uses of a polypeptide, and nucleic acid molecule
EP2096177A3 (en) 2004-12-17 2010-01-13 Metanomics GmbH Process for the production of lutein
CA2598792A1 (en) 2005-03-02 2006-09-08 Metanomics Gmbh Process for the production of fine chemicals
US20140199313A1 (en) 2005-03-02 2014-07-17 Metanomics Gmbh Process for the Production of Fine Chemicals
CN101137752B (en) 2005-03-08 2013-04-03 巴斯福植物科学有限公司 Expression enhancing intron sequences
CN101258246B (en) 2005-07-06 2012-09-05 克罗普迪塞恩股份有限公司 Plant yield improvement by ste20-like gene expression
US8071840B2 (en) 2005-09-15 2011-12-06 Cropdesign N.V. Plants having increase yield and method for making the same
ES2390919T3 (en) 2006-03-31 2012-11-19 Basf Plant Science Gmbh Plants that have improved performance-related traits and a method to make them
EP2090662A3 (en) 2006-04-05 2012-10-31 Metanomics GmbH Process for the production of a fine chemical
EP2497778A3 (en) 2006-05-31 2012-12-12 Metanomics GmbH Manipulation of the nitrogen metabolism
CN101460611B (en) 2006-06-08 2013-04-24 巴斯福植物科学有限公司 Plants having improved growth characteristics and method for making the same
EP2199395A1 (en) 2006-08-02 2010-06-23 CropDesign N.V. Plants transformed with SYT-polypeptide having increased yield under abiotic stress and a method for making the same
ITUD20060280A1 (en) 2006-12-29 2008-06-30 Univ Degli Studi Udine ARTIFICIAL SEQUENCE OF DNA EVENT FUNCTION OF LEADER IN 5 '(5'-UTR) OPTIMIZED FOR THE OVEREXPRESSION OF HETEROLOGICAL PLANT PROTEINS
US8426676B2 (en) 2007-05-04 2013-04-23 Basf Plant Science Gmbh Seed enhancement by combinations of pyruvate kinases
DE112008001453T5 (en) 2007-05-22 2010-04-29 Basf Plant Science Gmbh Plant cells and plants with increased tolerance and / or resistance to environmental stress and increased biomass production CO
CA2704271C (en) 2007-12-03 2016-07-19 Syngenta Participations Ag Engineering enzymatically susceptible phytases
AR069894A1 (en) 2007-12-21 2010-02-24 Basf Plant Science Gmbh PLANTS WITH GREATER PERFORMANCE PRODUCING A KNOCK OUT (SILENCING) IN GENES RELATED TO EFFICIENCY IN THE USE OF NITROGEN (KO NUE) "
WO2011074959A1 (en) 2009-12-15 2011-06-23 Edwin Henricus Antonius Holman Transgenic ozone-resistant plants
AR083029A1 (en) 2010-12-09 2013-01-23 Syngenta Participations Ag METHODS AND COMPOSITIONS THAT USE SMALL INTERFERENT ARN (ARNIP) FOR THE CONTROL OF NEMATODES IN PLANTS
WO2012099664A1 (en) 2011-01-18 2012-07-26 Syngenta Participations Ag Methods and compositions for modified ethanol inducible promoter systems
US8951752B2 (en) 2011-10-20 2015-02-10 University Of Iowa Research Foundation N-demethyase genes and uses thereof
BR112014020164B1 (en) 2012-02-16 2022-09-13 Syngenta Participations Ag MANIPULATED PESTICIDE VIP3 POLYPEPTIDE, NUCLEIC ACID MOLECULE, VECTOR AND COMPOSITION
WO2013187957A1 (en) 2012-06-15 2013-12-19 University Of Iowa Research Foundation Caffeine responsive promoters and regulatory genes
AR091512A1 (en) 2012-06-22 2015-02-11 Syngenta Participations Ag BIOLOGICAL CONTROL OF COLEOPTER PESTS
BR112015005389B1 (en) 2012-09-13 2022-10-18 Indiana University Research And Technology Corporation RECOMBINANT NUCLEIC ACID MOLECULE, SUBSTRATE PROTEIN MODIFIED OF A PLANT PATHOGEN SPECIFIC PROTEASE EXPRESSED THROUGH PLANT PATHOGEN, VECTOR, AND METHOD FOR PROTECTING A PLANT FROM INFECTION BY A PLANT PATHOGEN THAT SECRETS AT LEAST ONE SPECIFIC PROTEASE
BR112016023304A2 (en) 2014-04-17 2017-10-17 Boehringer Ingelheim Rcv Gmbh recombinant host cell engineered to overexpress helper proteins
KR102291978B1 (en) 2014-04-17 2021-08-23 베링거 인겔하임 에르체파우 게엠베하 운트 코 카게 Recombinant host cell for expressing protein of interest
EP2952584A1 (en) 2014-06-04 2015-12-09 Boehringer Ingelheim RCV GmbH & Co KG Improved protein production
US20170327834A1 (en) 2014-12-15 2017-11-16 Syngenta Participations Ag Pesticidal microrna carriers and use thereof
MX2017007904A (en) 2014-12-23 2017-09-05 Syngenta Participations Ag Biological control of coleopteran pests.
RU2762075C2 (en) 2015-04-10 2021-12-15 Зингента Партисипейшнс Аг Feed compositions for animals and methods for application thereof
WO2018076335A1 (en) 2016-10-31 2018-05-03 Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences Compositions and methods for enhancing abiotic stress tolerance
SG11201908079SA (en) 2017-03-29 2019-10-30 Boehringer Ingelheim Rcv Gmbh Recombinant host cell with altered membrane lipid composition
WO2018213771A1 (en) 2017-05-18 2018-11-22 Cargill, Incorporated Genome editing system
BR112020016306A2 (en) 2018-02-12 2020-12-15 Curators Of The University Of Missouri SMALL SUPRARREGULATED GENE (SAUR) FOR THE IMPROVEMENT OF THE PLANT'S RADICULAR SYSTEM ARCHITECTURE, FLOOD TOLERANCE, DROUGHT RESISTANCE, AND PERFORMANCE
KR20230144629A (en) 2021-02-12 2023-10-16 베링거 인겔하임 에르체파우 게엠베하 운트 코 카게 Signal peptide to increase protein secretion

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5447858A (en) * 1984-04-13 1995-09-05 Mycogen Plant Sciences, Inc. Heat shock promoter and gene
UA48104C2 (en) * 1991-10-04 2002-08-15 Новартіс Аг Dna fragment including sequence that codes an insecticide protein with optimization for corn, dna fragment providing directed preferable for the stem core expression of the structural gene of the plant related to it, dna fragment providing specific for the pollen expression of related to it structural gene in the plant, recombinant dna molecule, method for obtaining a coding sequence of the insecticide protein optimized for corn, method of corn plants protection at least against one pest insect
EP0637339B1 (en) * 1992-04-13 2001-10-31 Syngenta Limited Dna constructs and plants incorporating them
AU687961B2 (en) * 1993-11-19 1998-03-05 Biotechnology Research And Development Corporation Chimeric regulatory regions and gene cassettes for expression of genes in plants

Also Published As

Publication number Publication date
PL324880A1 (en) 1998-06-22
AR002914A1 (en) 1998-04-29
HUP9900057A3 (en) 2001-06-28
JPH11510694A (en) 1999-09-21
AU704172B2 (en) 1999-04-15
KR19990036251A (en) 1999-05-25
BR9609873A (en) 1999-03-23
TR199800177T1 (en) 1998-05-21
CA2227445A1 (en) 1997-02-20
CZ36998A3 (en) 1998-06-17
EP0846179A2 (en) 1998-06-10
WO1997006268A2 (en) 1997-02-20
IL123172A0 (en) 1998-09-24
MX9801008A (en) 1998-04-30
AU6625296A (en) 1997-03-05
GB9516241D0 (en) 1995-10-11
AP863A (en) 2000-08-04
SK16998A3 (en) 1998-09-09
WO1997006268A3 (en) 1997-03-13
NZ313724A (en) 1999-04-29
CN1199425A (en) 1998-11-18
AP9801194A0 (en) 1998-03-31

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