WO2001009357A3 - Ethanol inducible expression system - Google Patents

Ethanol inducible expression system Download PDF

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Publication number
WO2001009357A3
WO2001009357A3 PCT/GB2000/002750 GB0002750W WO0109357A3 WO 2001009357 A3 WO2001009357 A3 WO 2001009357A3 GB 0002750 W GB0002750 W GB 0002750W WO 0109357 A3 WO0109357 A3 WO 0109357A3
Authority
WO
WIPO (PCT)
Prior art keywords
promoter
alcr
protein
inducible
gene
Prior art date
Application number
PCT/GB2000/002750
Other languages
French (fr)
Other versions
WO2001009357A2 (en
Inventor
Ian Jepson
Andrew James Greenland
Mark Xavier Caddick
Arthur Brian Tomsett
Original Assignee
Syngenta Ltd
Ian Jepson
Andrew James Greenland
Mark Xavier Caddick
Arthur Brian Tomsett
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Ltd, Ian Jepson, Andrew James Greenland, Mark Xavier Caddick, Arthur Brian Tomsett filed Critical Syngenta Ltd
Priority to AU60012/00A priority Critical patent/AU6001200A/en
Publication of WO2001009357A2 publication Critical patent/WO2001009357A2/en
Publication of WO2001009357A3 publication Critical patent/WO2001009357A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline

Abstract

A chemically-inducible plant gene expression cassette is described. The expression cassette comprises a first promoter operatively linked to the alcR regulator protein sequence obtainable from Aspergillus nidulans which encodes the AlcR regulator protein, and an inducible promoter obtainable from the alcA gene promoter of Aspergillus nidulans operatively linked to a target gene, the inducible promoter being activated by the regulator protein in the presence of an effective exogenous inducer whereby application of the inducer causes expression of the target gene and wherein the alcA promoter has three or more binding sites for the AlcR protein. According to a preferred embodiment of the invention, the promoter comprises 4 direct repeat sequences for the AlcR protein.
PCT/GB2000/002750 1999-08-02 2000-07-18 Ethanol inducible expression system WO2001009357A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU60012/00A AU6001200A (en) 1999-08-02 2000-07-18 Expression system

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9918154.7A GB9918154D0 (en) 1999-08-02 1999-08-02 Expression system
GB9918154.7 1999-08-02

Publications (2)

Publication Number Publication Date
WO2001009357A2 WO2001009357A2 (en) 2001-02-08
WO2001009357A3 true WO2001009357A3 (en) 2001-10-11

Family

ID=10858410

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/002750 WO2001009357A2 (en) 1999-08-02 2000-07-18 Ethanol inducible expression system

Country Status (3)

Country Link
AU (1) AU6001200A (en)
GB (1) GB9918154D0 (en)
WO (1) WO2001009357A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE602004026742D1 (en) * 2003-09-02 2010-06-02 Martin Fussenegger REGULATORY GENE EXPRESSION IN MAMMALIC CELLS AND MAMMALITIES
US8237013B2 (en) 2004-07-08 2012-08-07 Dlf-Trifolium A/S Means and methods for controlling flowering in plants
EP2100961A1 (en) 2008-03-04 2009-09-16 Icon Genetics GmbH Method of protease production in plants
US9434953B2 (en) * 2011-01-18 2016-09-06 Syngenta Participations Ag Methods and compositions for modified ethanol inducible promoter systems
WO2018053006A1 (en) 2016-09-13 2018-03-22 Dana-Farber Cancer Institute, Inc. Methods and compositions for the positive selection of protein destabilizers

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993021334A1 (en) * 1992-04-13 1993-10-28 Zeneca Limited Dna constructs and plants incorporating them
WO1995022625A1 (en) * 1994-02-17 1995-08-24 Affymax Technologies N.V. Dna mutagenesis by random fragmentation and reassembly
WO1997006268A2 (en) * 1995-08-08 1997-02-20 Zeneca Limited Dna constructs
WO2000044917A1 (en) * 1999-02-01 2000-08-03 Syngenta Limited Inducible expression system for use in plants

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993021334A1 (en) * 1992-04-13 1993-10-28 Zeneca Limited Dna constructs and plants incorporating them
WO1995022625A1 (en) * 1994-02-17 1995-08-24 Affymax Technologies N.V. Dna mutagenesis by random fragmentation and reassembly
WO1997006268A2 (en) * 1995-08-08 1997-02-20 Zeneca Limited Dna constructs
WO2000044917A1 (en) * 1999-02-01 2000-08-03 Syngenta Limited Inducible expression system for use in plants

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KULMBURG PETER ET AL: "Specific binding sites for the activator protein, ALCR, in the alcA promoter of the ethanol regulon of Aspergillus nidulans.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 267, no. 29, 1992, pages 21146 - 21153, XP002159674, ISSN: 0021-9258 *
PANOZZO CRISTINA ET AL: "The zinc binuclear cluster activator AlcR is able to bind to single sites but requires multiple repeated sites for synergistic activation of the alcA gene in Aspergillus nidulans.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 272, no. 36, 1997, pages 22859 - 22865, XP002159675, ISSN: 0021-9258 *

Also Published As

Publication number Publication date
WO2001009357A2 (en) 2001-02-08
GB9918154D0 (en) 1999-10-06
AU6001200A (en) 2001-02-19

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