HUP0104675A2 - Eljárás sejtek automatikus mintavételére - Google Patents

Eljárás sejtek automatikus mintavételére

Info

Publication number
HUP0104675A2
HUP0104675A2 HU0104675A HUP0104675A HUP0104675A2 HU P0104675 A2 HUP0104675 A2 HU P0104675A2 HU 0104675 A HU0104675 A HU 0104675A HU P0104675 A HUP0104675 A HU P0104675A HU P0104675 A2 HUP0104675 A2 HU P0104675A2
Authority
HU
Hungary
Prior art keywords
pipette
cell
interface
suspension
cells
Prior art date
Application number
HU0104675A
Other languages
English (en)
Inventor
Nicholas Gerard Byrne
David Geraint Owen
Original Assignee
Cenes Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9826742.0A external-priority patent/GB9826742D0/en
Priority claimed from GBGB9905998.2A external-priority patent/GB9905998D0/en
Priority claimed from GBGB9906053.5A external-priority patent/GB9906053D0/en
Application filed by Cenes Limited filed Critical Cenes Limited
Publication of HUP0104675A2 publication Critical patent/HUP0104675A2/hu
Publication of HUP0104675A3 publication Critical patent/HUP0104675A3/hu

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48728—Investigating individual cells, e.g. by patch clamp, voltage clamp
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Sustainable Development (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Stored Programmes (AREA)
  • Advance Control (AREA)
  • Input Circuits Of Receivers And Coupling Of Receivers And Audio Equipment (AREA)
  • Measurement Of Current Or Voltage (AREA)
  • Road Signs Or Road Markings (AREA)
  • Discharging, Photosensitive Material Shape In Electrophotography (AREA)
  • Medicinal Preparation (AREA)

Abstract

A tal lm ny t rgya elj r s sejtek automatikus mintavételezésére,valamint az ehhez szükséges berendezés és a berendezés műk"dtetéséreszolg ló szab lyozó program. Az elj r sban a sejtek szuszpenziój ttartalmazó kapill ris cső és a mintavételezésre szolg ló foltpipettafüggőleges helyzetben, koncentrikusan vannak a tartószerkezetükrefelszerelve. A sejtek a szuszpenzióban kiülepednek, ez ltalfeldúsulnak folyadék/levegő hat rfelületen. A pipett t érintkezésbehozva a hat rfelülettel a pipetta hegyéhez egy sejtet r"gzíthetnek,úgy, hogy az gigaOhmos elektromos ellen ll st biztosítson. A sejtetk"rülvevő oldat cseréjével a gyógyszerek ioncsatorn kra gyakorolt hatsa vizsg lható. Ó
HU0104675A 1998-12-05 1999-12-06 Interface patch clamping HUP0104675A3 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GBGB9826742.0A GB9826742D0 (en) 1998-12-05 1998-12-05 Interface patch clamping
GBGB9905998.2A GB9905998D0 (en) 1999-03-17 1999-03-17 Interface patch clamping
GBGB9906053.5A GB9906053D0 (en) 1999-03-17 1999-03-17 Interface patch clamping
PCT/GB1999/004073 WO2000034776A1 (en) 1998-12-05 1999-12-06 Interface patch clamping

Publications (2)

Publication Number Publication Date
HUP0104675A2 true HUP0104675A2 (hu) 2002-04-29
HUP0104675A3 HUP0104675A3 (en) 2005-10-28

Family

ID=27269575

Family Applications (1)

Application Number Title Priority Date Filing Date
HU0104675A HUP0104675A3 (en) 1998-12-05 1999-12-06 Interface patch clamping

Country Status (13)

Country Link
US (1) US7384733B1 (hu)
EP (1) EP1141704B1 (hu)
JP (1) JP4335460B2 (hu)
KR (1) KR100736059B1 (hu)
AT (1) ATE513209T1 (hu)
AU (1) AU775985B2 (hu)
CA (1) CA2352877A1 (hu)
HU (1) HUP0104675A3 (hu)
IL (2) IL143213A0 (hu)
MX (1) MXPA01005582A (hu)
NO (1) NO20012766L (hu)
PL (1) PL348035A1 (hu)
WO (1) WO2000034776A1 (hu)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9812783D0 (en) 1998-06-12 1998-08-12 Cenes Ltd High throuoghput screen
CA2352877A1 (en) 1998-12-05 2000-06-15 Cenes Limited Interface patch clamping
GB0006748D0 (en) * 2000-03-21 2000-05-10 Cenes Ltd Improved interface patch clamping
GB0013584D0 (en) * 2000-06-06 2000-07-26 Cenes Ltd Automated flow patch-clamp system
DE10032568A1 (de) * 2000-07-05 2002-01-24 Nmi Univ Tuebingen Vorrichtung und Verfahren zum elektrischen Kontaktieren von in einer Flüssigkeit in Suspension befindlichen biologischen Zellen
US6815197B2 (en) * 2001-02-16 2004-11-09 Multi Channel System Mcs Gmbh Apparatus for conducting electrophysiological measurements on cells
GB0107231D0 (en) 2001-03-22 2001-05-16 Imp College Innovations Ltd Patch clamp
DE10130218A1 (de) * 2001-06-22 2003-01-09 Infineon Technologies Ag Vorrichtung für ein Patch-Clamping von Vesikeln und Verfahren zu deren Herstellung
AU2002352989A1 (en) * 2001-11-30 2003-06-17 Bristol-Myers Squibb Company Liquid interface configurations for automated patch clamp recording
WO2003048786A2 (en) 2001-11-30 2003-06-12 Bristol-Myers Squibb Company Pipette configurations and arrays thereof for measuring cellular electrical properties
DE10202887B4 (de) * 2002-01-25 2004-05-06 Advalytix Ag Zellanalyseverfahren
ES2208082B1 (es) * 2002-05-29 2005-09-16 Jose Luis Bardasano Rubio Dispositivo para la medida del potencial de la accion transmembrana en preparaciones celulares bajo la accion de campos electromagneticos de frecuencia e intensidad variables.
US8202439B2 (en) 2002-06-05 2012-06-19 Panasonic Corporation Diaphragm and device for measuring cellular potential using the same, manufacturing method of the diaphragm
JP4552423B2 (ja) 2003-11-21 2010-09-29 パナソニック株式会社 細胞外電位測定デバイスおよびこれを用いた細胞外電位の測定方法
US8232074B2 (en) 2002-10-16 2012-07-31 Cellectricon Ab Nanoelectrodes and nanotips for recording transmembrane currents in a plurality of cells
FR2866958B1 (fr) * 2004-02-26 2006-08-04 Commissariat Energie Atomique Procede et dispositif de controle du positionnement d'un element biologique sur un support
KR100749908B1 (ko) * 2005-07-28 2007-08-21 한국과학기술연구원 패치 클램프 시스템 및 이를 이용한 세포 밀봉 방법
US20120019270A1 (en) * 2010-07-21 2012-01-26 Amodei Dario G Microfabricated pipette and method of manufacture
EP2710109B1 (de) * 2011-05-19 2017-10-25 NMI Naturwissenschaftliches und Medizinisches Institut an der Universität Tübingen Verfahren und verwendung einer vorrichtung zur automatisierten positionsbestimmung eines mikrosystems zur manipulation eines sphärischen mikro-objekts
CN102866247A (zh) * 2012-09-26 2013-01-09 东南大学 一种全自动膜片钳电生理纪录系统及记录方法
CN113430105A (zh) * 2015-04-08 2021-09-24 贝克顿·迪金森公司 用于从半固体表面收集微生物生长的设备和装置
CN108387400A (zh) * 2018-05-28 2018-08-10 南京信息工程大学 一种沉积物孔隙水原位高分辨率采样器
CN109374875A (zh) * 2018-11-20 2019-02-22 中国科学院生物物理研究所 低噪恒温重力灌流膜片钳装置
CN112014429B (zh) * 2019-05-30 2024-01-30 华东理工大学 一种基于超微电渗流调控的细胞膜振动检测方法

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4897426A (en) 1986-03-06 1990-01-30 New York University Method for blocking calcium channels
WO1989001159A1 (en) 1987-07-27 1989-02-09 Commonwealth Scientific And Industrial Research Or Receptor membranes
US5225374A (en) 1988-05-13 1993-07-06 The United States Of America As Represented By The Secretary Of The Navy Method of fabricating a receptor-based sensor
DE68926118T2 (de) 1988-08-18 1996-08-22 Australian Membrane And Biotechnology Research Institute Ltd. Commonwealth Scientific And Industrial Research Organization, North Ryde, Neusuedwales Verbesserungen an der empfindlichkeit und der selektivität von ionenkanalmembranbiosensoren
US5643796A (en) 1994-10-14 1997-07-01 University Of Washington System for sensing droplet formation time delay in a flow cytometer
CA2203886C (en) 1994-10-28 2003-09-02 Soren-Peter Olesen Patch clamp apparatus and technique having high throughput and low fluid volume requirements
ATE254965T1 (de) 1995-04-25 2003-12-15 Discovery Partners Internation Fernprogrammierbare matrizen mit speichern und verwendungen davon
US5874668A (en) * 1995-10-24 1999-02-23 Arch Development Corporation Atomic force microscope for biological specimens
WO1997020203A1 (de) 1995-11-28 1997-06-05 Thomas Schalkhammer Neuartige membranen und membran-dna/rna-sensoren
WO1997025616A1 (en) 1996-01-11 1997-07-17 Australian Membrane And Biotechnology Research Institute Ion channel sensor typing
JPH09211010A (ja) 1996-02-06 1997-08-15 Bunshi Bio Photonics Kenkyusho:Kk 電気生理特性測定装置
WO1998022819A1 (de) * 1996-11-16 1998-05-28 Nmi Naturwissenschaftliches Und Medizinisches Institut An Der Universität Tübingen In Reutlingen Stiftung Bürgerlichen Rechts Mikroelementenanordnung, verfahren zum kontaktieren von in einer flüssigen umgebung befindlichen zellen und verfahren zum herstellen einer mikroelementenanordnung
DE19712309A1 (de) 1996-11-16 1998-05-20 Nmi Univ Tuebingen Mikroelementenanordnung, Verfahren zum Kontaktieren von in einer flüssigen Umgebung befindlichen Zellen und Verfahren zum Herstellen einer Mikroelementenanordnung
ATE322010T1 (de) 1996-11-29 2006-04-15 Anordnungen von unabhängig voneinander ansteuerbaren, gestützten flüssigbilayer- membranen und ihre anwendungsverfahren
ATE349694T1 (de) * 1997-05-01 2007-01-15 Sophion Bioscience As Vorrichtung zur automatischen elektrodenpositionierung
US5981268A (en) 1997-05-30 1999-11-09 Board Of Trustees, Leland Stanford, Jr. University Hybrid biosensors
SE9702112D0 (sv) * 1997-06-04 1997-06-04 Holdingbolaget Vid Goeteborgs Method and apparatus for detection of a receptor antagonist
DE19744649C2 (de) 1997-10-09 2003-03-27 Fraunhofer Ges Forschung Verfahren zur Messung bioelektrischer Signale von Zellen nach der Patch-Clamp-Methode sowie Verwendung einer Vorrichtung hierzu
DE19841337C1 (de) 1998-05-27 1999-09-23 Micronas Intermetall Gmbh Verfahren und Vorrichtung zur intrazellulären Manipulation einer biologischen Zelle
GB9812783D0 (en) 1998-06-12 1998-08-12 Cenes Ltd High throuoghput screen
CA2352877A1 (en) 1998-12-05 2000-06-15 Cenes Limited Interface patch clamping
GB0006748D0 (en) 2000-03-21 2000-05-10 Cenes Ltd Improved interface patch clamping
GB0013584D0 (en) 2000-06-06 2000-07-26 Cenes Ltd Automated flow patch-clamp system

Also Published As

Publication number Publication date
IL143213A0 (en) 2002-04-21
NO20012766L (no) 2001-08-06
JP2002532684A (ja) 2002-10-02
CA2352877A1 (en) 2000-06-15
JP4335460B2 (ja) 2009-09-30
EP1141704B1 (en) 2011-06-15
WO2000034776A1 (en) 2000-06-15
KR100736059B1 (ko) 2007-07-06
NO20012766D0 (no) 2001-06-05
IL143213A (en) 2010-11-30
KR20010090865A (ko) 2001-10-19
AU775985B2 (en) 2004-08-19
AU1575400A (en) 2000-06-26
ATE513209T1 (de) 2011-07-15
MXPA01005582A (es) 2002-07-02
HUP0104675A3 (en) 2005-10-28
PL348035A1 (en) 2002-05-06
EP1141704A1 (en) 2001-10-10
US7384733B1 (en) 2008-06-10

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Legal Events

Date Code Title Description
HC9A Change of name, address

Owner name: XENTION LIMITED, GB

Free format text: FORMER OWNER(S): CENES LIMITED, GB; XENTION DISCOVERY LIMITED, GB

FD9A Lapse of provisional protection due to non-payment of fees