HUE031986T2 - Stressz-rezisztens növények és azok elõállítása - Google Patents

Stressz-rezisztens növények és azok elõállítása Download PDF

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HUE031986T2
HUE031986T2 HUE11781777A HUE11781777A HUE031986T2 HU E031986 T2 HUE031986 T2 HU E031986T2 HU E11781777 A HUE11781777 A HU E11781777A HU E11781777 A HUE11781777 A HU E11781777A HU E031986 T2 HUE031986 T2 HU E031986T2
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plant
gin
glu
gene
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Pascal Gantet
Emmanuel Guiderdoni
Ngangiang Khong
Jean-Benoit Morel
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Inst Nat De La Rech Agronomique (Inra)
Centre De Coop Int En Rech Agronomique (Cirad)
Univ Montpellier
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    • C12N15/8281—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for bacterial resistance
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    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
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    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance

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Claims (7)

  1. Étressz -tplMfiős miimnk is wmk eíőitfiása Izibadalmí: igénypontök
    1, Egyszíkűekhest tartozó, RNS interferencia Üti! okozott. gib-eihaHgattatás kö*' vetkeztében Élteié MADS26 funkciójú növény, mely bíóilkos éf|ya§y abiotdus stpiiszi! stetíban megnövelt razlsztendát riutat. I* Az :!.. igénypont szerinti növény, ahol a növény rizs^btza* árpa, Aok is kpkonca közül választott gabonaféle. 3> A 2- igénypont szerion növény magja,
  2. 4, Az. l~3. igénypontok bármelyike szennti növény, ahol a biotikus scresszszaf szembeni resztének) gomba és/vsgy bakteriális patogénekkel szemben mutatott rezisztencia. 5. A4. Igénypont szerinti növiny, ahol a górni# fptoginei MfÉÉipó/táé* Puca nia, Ustüago, Septoria, Ensyphe, Rhimctépm #1 PP$&rimg tirpák közöl ke-rölnek ki,, előnyösen a ;$i, Ê *k, igénypont szerinti növény, ahol a bakteriális pafcogének Xanthamor\asf: RaMania? Emmám* Pectúlmct&iuni, R&níma, Agrobactedum* Psrndomom^ Burkimidana, Miénmm% Cki/i&mtéi, Stmptomycas, Xyiéiia^ Bpimpiasma és RkympMama species törzsek közöl kerülnek f% előnyösen a pafogén .ka iïëmmùnm oryzae,
    2, Az i, igénypont szennti növény, ateölkaz őblöiitM ifölSszÉí síimieoi elsz--tencia szárazság! stresszel szemben! rezisztencia.
  3. 8.. Éljárás egysziki nöyényekiéö g«li és/vagy bgperills. pgtoféoek áltál okozott stressz és/vagy szárazság! stresszel «mbani rezisztencia Okozására, melynek során; (a) egy növény; sejtben vagy oignon o M.AOS26 gén funkciói inokilvahuk, (b) adott esetben szelektáljuk ok (a) lépés szerinti, toaktiváít HADS26 gén funkvr dóval rendelkező növényi sejteket; ín) az (a) vagy (ö) lépésekből származó sejtekből regeneráljuk a növényeket; és (d) adott esetben a (e) lépésből származó, gomba és/vagy bakteriális patogének által okozott stressz és/vagy szárazság* stresszel széniben* rezisztenciával rendelkező, hsbée MAPS.26 gen Imkcioiú növényt szeektálju^. Ér A s, igénypont szerint; eljárás, aboi á kiAD§26 gén: lankad inaktiválása iéíédóvaí, egy vagy több nukieotid Inszartáiásával és/vá|p #ypœs$fêiiiWù hiiy-'Specifikus mutagenézissei, etilarmtähszukonit motigaoizissel :ge- nomoköan targebnggei »odúkéit hely* iézlékkal (T!LÜ®|y knoők-out: téctoikák-kai, vagy RNS interferenciával indult gén'elhallgattatással éörtihík, 10. A 8. vagy 9. igénypont szerint* eljárás/illbh (a) magokban a d.ADS26 gén funkciót mutagenézíssel ináktiváljuk; fb) ez (a) lépés szerinti magokból hátai növényeket hozunk létre; és (c> a (b) lépés szerint létrehozott, hibás M.ADS2S gén funkcióval rendelkező fiatal nővén yeket sze lek lé i j u k.
  4. 11. A ielft: Iplnypontok bármelyike szerinti eljárás, ahol: a növény egy Ppaceae családba tartozó, előnyösen nzs, búza, árpa, zab, rozs, cirok vagy kukorica közül yÉiasztofligyszlki* 12. £gy RNSi molekula, mely az MAD26 mRNS szekvenciához kötődik, és gátolja a NAD26 gén kifejeződését, mely rnSí adott esetben komplementé?' a 16, számú szekvenciát (GSTl) vagy 17. számú szekvenciát (GST2) tails;mazó szekvencia-hoz,
  5. 13. Egy RNSs molekula 12, igénypont szerinti alkalmazása, egyszikőekhez tartozó növények vagy növényi sejtek biohkus vagy abiofckus stresszel szembeni rezisz-tendájának fokozására.
  6. 14. Eljárás gomba és/vagy bakteriális patogenek által okozott stresszel és/vagy szárazság! stresszel szemben fokozottan ellenálló egyszikű növény eiőáihtására, melynek során: (a) egy növényben vagy magban a HADS26 gén funkciót RNS interferenciával mdukált gén-eioaligattatássai ^aktiváljuk, (b) adott esetben szelektáljuk az (a) lépés szénné, maktivált MAOS26 gén fűnk-dévai rendelkező növényi sejteket; (c) az (a) vagy (b) lépésekből származó sejtekből regeneráljuk a növényeket; és (d) adott esetben a (c) lépésből származó, gomba és/vagv bakteriális patogének által okozott stresszel és/vagy szárazság* stresszel szemt|pi rezisztenciával réfn ÍelRezÉ| RNS iotetférihiiivai indukált géme! lia 11 g a ttatá ssa ! Inaktivált hibás MÄDS26 gén funkciójú növényt szelektáljuk,
  7. 15. Egyszikőekhez tartozó növény, mely egy,, a MÄD26 mRNS szekvenciához kö tődő, és adott esetben a 16. számú szekvenciát (GSTl) vagy 17. számú szekvenciát (GST2) tartalmazó szekvenciához kompiimenter, a HálŐSif géb kifejem zödését gátié rnhí. vekmrM
HUE11781777A 2010-11-04 2011-11-03 Stressz-rezisztens növények és azok elõállítása HUE031986T2 (hu)

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CA2970138A1 (en) * 2014-12-17 2016-06-23 Pioneer Hi-Bred International, Inc. Modulation of yep6 gene expression to increase yield and other related traits in plants
KR101775789B1 (ko) * 2015-10-27 2017-09-12 중앙대학교 산학협력단 고추 유래의 건조 저항성 관련 단백질 ｍａｆ1 및 이의 용도
AU2019255192B2 (en) 2018-04-18 2023-02-02 Pioneer Hi-Bred International, Inc. Genes, constructs and maize event DP-202216-6
KR102156406B1 (ko) * 2018-12-14 2020-09-15 대한민국 스트레스 저항성이 향상된 식물체의 제조방법
CN110437324A (zh) * 2019-07-31 2019-11-12 琼台师范学院 香蕉枯萎病菌转录因子FoRlm1及其应用
CN110804623A (zh) * 2019-11-28 2020-02-18 中国农业科学院作物科学研究所 小麦TaMADS6基因在调控植物穗和籽粒发育以及开花时间中的应用
CN112159464B (zh) * 2020-09-28 2022-07-01 中国农业科学院作物科学研究所 一种小麦TaSEP基因及其在调控生长和发育中的应用
CN112760322B (zh) * 2021-02-07 2023-01-31 福建省农业科学院生物技术研究所 一种水稻组成型强启动子及其应用
CN114176084B (zh) * 2021-12-06 2022-09-20 南京天秾生物技术有限公司 2-氨基-3-羟基-3-甲基丁酸和/或2-氨基-3-(4-羟基苯基)丁酸的应用

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