HRP940938A2 - Pyrone derivatives as protease inhibitors and antiviral agents - Google Patents

Description

Field of invention

The present invention relates to pyrone derivatives that inhibit aspartic proteases, especially aspartic protease found in retroviruses, including the human immunodeficiency virus (HIV). It is expected that the pyrones can be used as antiviral agents for the treatment of infections caused by HIV or other retroviruses that use aspartate protease, and to be useful in the treatment of diseases caused by retroviruses, including AIDS.

So far known later

The name for acquired immunodeficiency syndrome (AIDS) was proposed in 1982 with the aim of describing the clinical manifestations of immunodeficiency. The etiological agent for AIDS was only later associated with retroviruses, the human immunodeficiency virus (HIV), or the lentivirus subfamily. At least two infectious strains of HIV have been identified, namely HIV-1 and HIV-2. In this proposal, the term HIV will be used as a general term that refers to all strains and mutants of the human immunodeficiency virus. A detailed study of the properties of HIV has enabled various approaches to the development of antiviral drugs, including the inhibition of the viral aspartate protease. (D. Richman, Control of Viral Diseases, 45th Symposium of the Society for General Microbiology, 261-313, (1990).

Aspartate proteases can be found in many retroviruses, including feline immunodeficiency virus (HV), myeloblastosis-associated virus (MAV), and Rous sarcoma virus (RSV) [H. Toh etal. Nature, 315: 691 (1985); J. Kay, and B.M. Dunn, Biochim. Biophys. Acta, 1: 1048 (1990); C. Cameron, J. Biological. Chem., 168: 11711-720 (1993)]. Since there are structural similarities between known retroviral proteases, compounds that inhibit proteases from HIV may also inhibit proteases from other retroviruses.

Aspartate protease from HIV is responsible for the post-translational shaping of viral polyprotein precursors such as pol and gag. [M. Graves, Structure and Function of Aspartic Proteases, 395-405 (1991)]. Degradation of these polyproteins is fundamental for the maturation of the virus, and the proteolytic activity necessary for the formation of polyproteins cannot be carried out by the action of the host's cellular enzymes. It was found that viruses that do not contain these proteases, as well as mutants in which the protease is inactive, are not infectious [C.Ping et al., J. Virol, 63: 2550-556 (1989) and N. Kohl et al, Proc. Nali. Acad. Sci. USA, 85: 4686-90 (1987)]. Thus, it was established that selective inhibitors of HIV proteases inhibit the spread of the virus and the formation of cytopathological consequences in the culture of acutely infected cells. [(J. C. Craig, Antiviral Research, 16: 295-305 (1991)] For these reasons, inhibition of HIV proteases is considered a promising approach to antiviral therapy.

HIV protease inhibitors have been extensively reviewed in [e.g. A: Tomasselli et al, Chimica Oggi, 6-27 (1991) and T. Meek, J. Enzyme Inhibition. 6: 65-98 (1992)]. However, most of the mentioned inhibitors are peptides and therefore unsuitable as drugs, due to the well-known pharmacological disadvantages that occur with most peptide drugs (excretion via bile, low bioavailability and instability in the physiological environment, etc.). Non-peptide inhibitors of HIV proteases are therefore very important as they can be used as useful therapeutic agents.

Patent Hei 3-227923 claims that coumarins have anti-HIV activity. However, only 4-hydroxycoumarin was specifically described, but without discussion of the mechanism of action.

World Patent 89/07939 lists eight coumarin derivatives as HlV reverse transcriptase inhibitors with potential antiviral activity. These derivatives are hexachlorocoumarin, 7-acetoxycoumarin, whose structures are shown in the following figure.

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Warfarin, (3-(α-acetonylbenzyl)-4-hydroxycoumarin), shown below was presented by R. Nagorny et al. uAIDS 7: 129-130 (1993) as an inhibitor of cell-mediated and non-cell-mediated HIV infections. However, warfarin was the only pyrone analog investigated, and the mechanism of its action in inhibiting HIV was not specified.

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It has been shown by Fairli et al., (Biochem. Biophjs. Res. Comm., 188: 631-637, (1992) that selected flavones, which are structurally different from the pyrones of the present invention, inhibit HIV-1 protease. These compounds are shown below.

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United States Patent No. 3,206,476 describes several pyrones in particular 3-substituted-4-hydroxy-6-aryl-2-pyrones as pressure lowering agents. However, the type of these substituents in position 3 in these heterocycles is limited only to halogens and amino groups, and alkanoylamino derivatives.

United States Patent No. 3,818,046 describes several pyrone derivatives, particularly 4-hydroxypyrones with carbon chains containing sulfur in the 3-position as growth inhibitors and antimicrobial agents. Substituents in the 5-position of these heterocyclic compounds are limited to methyl groups. The pyrones shown below are substituted as follows: R=Me; M=H or alkali metal; R'=H, alkyl, phenyl, halophenyl, nitrophenyl, lower alkylphenyl, benzyl, phenethyl, naphthylmethyl, halobenzyl, lower alkylbenzyl, nitrobenzyl, propargyl, allyl, cyclohexyl, lower alkyl, lower thioalkyl or adamantyl; and n=0 to 2.

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The pyrone preparation process is shown in United States Patent No. 3,931,235.

Summary of the invention

The present invention is based in large part on the inventor's special discoveries that novel tri- and tetrasubstituted pyrone derivatives, as well as corresponding compounds selected from a wide range of "tailored" molecular structures, potentially inhibit HIV aspartyl protease thereby blocking infection caused by HIV V. Presented the invention is also based on the knowledge of the mechanism of action of antiviral drugs, especially as shown in the structure-activity relationship characteristic of ani-HIV compounds that include pyrone derivatives Zla The discovered pyrones are expected to be extremely useful in the development of treatment of infections caused by viruses, especially retroviruses whose replication and activation are based on the activity of aspartate protease. One such retrovirus is HIV. For these reasons, it is expected that pyrones with antiviral activity may be useful in the treatment of diseases and syndromes associated with viral pathogenesis. One such syndrome is AIDS.

The proposed efficient synthesis of biologically active pyrones involves either de novo constitution of pyrone cores or modification of pyrones with suitable functionality. Furthermore, many of the given working examples show the preparation of specific pyrones whose structures contain the desired functional groups with the appropriate geometry.

The testing of specific pyrones as inhibitors of aspartate protease from HIV, based on testing the hydrolysis of undecapeptide enzyme substrates, and the testing of pyrones as inhibitors of virus growth and infectivity, based on testing the infection of the H9 cell line with the HIV-1 virus, are also covered by this invention. Surprisingly strong inhibition of the enzyme, at nanomolar concentrations, with corresponding anti-HIV activity was observed.

The present inventors contemplate the preparation of pharmaceutically useful antiviral compositions comprising one or more of the disclosed pyrones and corresponding compounds with pharmaceutically compatible excipients. They are also considering the use of these preparations alone or in combination with other antiviral treatments, in the treatment of infections and diseases caused by retroviruses, including AIDS.

The present invention relates to the compounds or their pharmaceutically compatible salts of formula 1 shown below.

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where

X represents OR 1 , NHR 1 , SR 1 , CO 2 R 4 , CH 2 OR 1 , wherein R 1 represents R 4 or COR 4 and R 4 is as defined below;

Y represents oxygen or sulfur;

Z represents rain or sulphur;

A and A1 are independently chemical bonds, unsubstituted or substituted phenyl, naphthyl, five- or six-membered heterocyclic ring, cycloalkyl or fused ring system of 8 to 10 atoms or their substituted derivative, whereby the substituents can be one or more of the following groups: F , Cl, Br, OR4, N(R4)2, CO2N(R4)2, CO2R4, COR4, R4, OCH2O, OCH2CH2O or CHN, where R4 independently represents hydrogen, substituted or unsubstituted alkyl, cycloalkyl, alkylcycloalkyl or phenyl, wherein the substituents can be one or more of the following groups: CO2R2, CON(R2)2, F, OR2, SR2, N(R2)2, CN, phenyl, naphthyl, heterocyclyl or CF3, and R2 independently represents alkyl, cycloalkyl or hydrogen;

R5 represents hydrogen, alkyl, cycloalkyl, alkylcycloalkyl, phenyl or their substituted derivatives, and the substituents can be one or more of the following groups: CO2R2, CON(R2)2, F, OR2, phenyl, naphthyl, CF3, OR1, NHR1, SR1 , or CH2OR2, wherein R1 is as defined above;

R3 is independently hydrogen, (CH2)pR4 or (CH2)pA where p is an integer from 0 to 2 and R4 and A are as defined above;

W, W1, and W4 each independently represent a chemical bond, oxygen, NR3, C(R3)2, CO, CR3=CR3, C≡C, OR3OR3, C(=NR3)NR3, S(O)p, CR3N(R3 )2, SO2NR3, CO2, NR3COVgA, and NCOVgR3, where g is 0 or 1, and V can be oxygen, sulfur, NR3 or CHR3;

W2 represents oxygen, NR3, S(O)p, SO2NR3, -OCO, NR3COVgA, and NCOVgR3, where g is 0 or 1, and V can be O, S, NR3 or CHR3;

m and n each represent an integer from 0 to 4 with the restriction that if W and W1 are heteroatoms, or if W2 and W3 are heteroatoms, m is an integer from 2 to 4; and with the further restriction that R3W1'(CH2)mW(CH2)nA cannot be methyl or ethyl.

Preferred compounds of formula 1 from the present invention are those in which X represents hydroxyl, amino or hydroxylmethyl; Y represents oxygen; Z represents oxygen or sulfur;

W, W1, and W4 each independently represent oxygen, NR3, NCOVgR3, CR3=CR3, SO2NR3, sulfur or C(R3)2, and W2 is one of the following groups: O, NR3, S, and NCOVgR3, where V can be oxygen , sulfur, NR3 or CHR3, and R3 is independently hydrogen, (CH2)pR4 or (CH2)pA, where p is an integer from 0 to 2, g is an integer from 0 to 1, and A independently represents phenyl, naphthyl, a five- or six-membered heterocyclic ring having one or two heteroatoms, a fused ring system of 8 to 10 atoms, cyclopentyl, cyclohexyl or their substituted derivatives, where the substituents can be one or more of the following groups: F, Cl, Br, OR4, N (R4)2, CO2R4, CON(R4)2, R4, OCH2O or OCH2CH2O, where R4 independently represents hydrogen, straight-chain or branched alkyl of 1 to 5 atoms, cycloalkyl group of 3 to 6 carbon atoms, CH2-cycloalkyl group of 4 to 8 carbon atoms, phenyl or its substituted derivative in which the substituents can be: CO2R2, F, OR2, phenyl or CF3, where R2 represents hydrogen, methyl, ethyl, isobutyl, t-butyl or cycloalkyl containing 3 to 6 carbon atoms, wherein A1 is as previously defined; you

R5 represents hydrogen, methyl, ethyl, propyl, cyclopropyl, hydroxyl, carboxyl or hydroxymethyl.;

Even more preferred compounds of formula 1 from the present invention are those in which:

X represents hydroxyl;

Z represents oxygen;

Y represents oxygen;

W, W1, and W4 each independently represent oxygen, sulfur, SO2NR3, NR3, or C(R3)2, CR3=CR3, and W2 represents O, NR3, S, wherein R3 independently represents hydrogen, (CH2)pR4, or (CH2 )pA, and p is an integer from 0 to 2, g is an integer from 0 to 1, and R4 represents hydrogen, methyl, ethyl, isopropyl, isobutyl, cyclopropyl, cyclohexyl, cyclopropylmethyl, cyclohexylmethyl, CH2CO2R2, phenyl or benzyl; R 2 is H, methyl, ethyl, isobutyl or t-butyl; A represents phenyl, 2,3- or 4-pyridyl, 2,4- or 5-thiazolyl, morpholinyl, 2- or 3-furyl, cyclopentyl, cyclohexyl, indalyl or substituted derivatives thereof, cyclopentyl, cyclohexyl, indalyl or substituted derivatives thereof , the substituents can be the following groups: F, Cl, Br, OR4, R4, CO2R4 or OCH3=, where A1 is as previously defined; you

R 5 represents hydrogen, methyl, ethyl, or hydroxymethyl.

Some of the most preferred compounds of the present invention include:

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Detailed description of the invention

The term "alkyl" usually denoted by "R" denotes a straight or branched chain hydrocarbon radical which, unless otherwise specified, contains from 1 to 12 carbon atoms, and includes, for example, methyl, ethyl, butyl, n-propyl, isopropyl, n-butyl, s-butyl, isobutyl, tert-butyl, n-pentyl, n-hexyl, n-heptyl, n-octyl, n-nonyl, n-decyl, udecyl and dodecyl. Alkyl groups can have one or more sites of unsaturation, such as double and triple carbon-carbon bonds. The alkyl group is substituted or unsubstituted, and it can be with 1 to 3 of the following substituents: alkyl, alkoxy, thioalkoxy, all as defined here, hydroxy, thiol, nitro, halogen, amino, formyl, carboxyl, nitrile, -NH-CO -R, -CO-NH-, -CO2R, -COR, aryl or heteroaryl wherein alkyl (R), aryl and heteroaryl are also as defined herein.

The term "cycloalkyl" also represented by "R" means, unless otherwise specified, a hydrocarbon ring containing 3 to 12 carbon atoms, for example cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl and adamantyl. When possible, the cycloalkyl group may contain a double bond. The cycloalkyl ring may be unsubstituted or substituted with 1 to 3 of the following groups: alkyl, alkoxy, thioalkoxy, all of which are as defined herein, hydroxy, thiol, nitro, halogen, amino, formyl, carboxyl, nitrile, -NH-CO -R, -CO-NHR-, -CO2R, -COR, aryl or heteroaryl, wherein alkyl (R), aryl and heteroaryl are as defined herein.

The terms "Alkoxy" and "ThioAlkoxy" refer to O-alkyl or S-alkyl, and alkyl is as defined above.

The term spirocyclyl refers to a carbocyclic or heterocyclic ring whose ends meet at a single carbon atom of the chain or other ring. Examples of such spirocyclyls are rings A in the following structures:

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The term "aryl" refers to an aromatic radical that is a phenyl group, a benzyl group, a naphthyl group, a biphenyl group, a pyrenyl group, an anthracylic group, a fluarenyl group, or fused rings derived from two phenyl, naphthyl, and five- or four-membered rings containing 0 to 3 heteroatoms, and may be selected from quinoline, isoquinolone, indole, indane, benzofuran, benzothiophene, benzoxazole, benzimidazole and the like, and may be unsubstituted or substituted with 1 to 3 of the following substituents: alkyl as defined above, alkoxy as is defined above, thioalcos as defined above, hydroxy, thiol, nitro, halogen, amino, formyl, carboxy, nitrile, -NHCOR, -CONHR, -CO2R, -COR, aryl or heteroaryl, wherein alkyl (R) is , aryl and heteroaryl as defined above.

The terms "heteroaryl" and "heterocyclyl" usually represented by "Ar" refer to a cyclic radical containing a heteroatom which is 2- or 3-thienyl, 2- or 3-furanyl, 2- or 3-pyrrolyl, 2-, 4- or 5-imidazolyl, 3-, 4- or 5-pyrazolyl, 2-, 4- or 5-thiazolyl, 3-, 4- or 5-isotoazolyl, 2-, 4- or 5-oxazolyl, 3-, 4- or 5-isoxazolyl, 3- or 5- 1,2,4-triazolyl, 4- or 5- 1,2,3-triazolyl, tetrazolyl, 2-, 3- or 4-pyridinyl, 3-, 4- or 5 -pyridazinyl, 2-pyrazinyl, 2-,4- or 5-pyrimidinyl, 2-, 3-, 4-, 5-, 6-, 7- or 8-quinolinyl, 1-, 3-, 4-, 5- , 6-, 7- or 8-isoquinolinyl, 2-, 3-, 4-, 5-, 6- or 7-indolyl, 2-, 3-, 4-, 5-, 6- or 7-benzo[b ]thienyl, 2-, 4-, 5-, 6- or 7-benzoxazolyl, 2-, 4-, 5-, 6- or 7-benzimidazolyl, 2-, 4-, 5, 6- or 7-benzothiazolyl, 1- or 2-piperazinyl, 2-, 3- or 4-morpholinyl, 2-, 3- or 4-thiomorpholinyl, 1-, 2- or 3-pyrrolidinyl, 2- or 3-tetrahydrofuranyl, 1-, 2-, 3 -, 4-, 5-, 6-, 7- or 8-tetrahydroquinolinyl and the like, and may be unsubstituted or substituted with 1 or 2 substituents which and may be alkyl as defined above, aryl as defined above, alkoxy as defined above, thioalkoxy as defined above, hydroxy, thiol, nitro, halogen, formyl, amino, carboxyl, nitrile, -NHCOR, - CO2R, -COR wherein alkyl is as defined above.

"Halogen" means fluorine, chlorine, bromine or iodine.

Some compounds of formula 1 can further form pharmaceutically compatible salts formed by acid and/or base addition. All these forms are covered by this invention.

Pharmaceutically compatible acid addition salts of formula 1 are derived from non-toxic inorganic acids such as hydrochloric, nitric, phosphoric, sulfuric, hydrobromic, hydroiodic, hydrofluoric, phosphoric acid and the like, as well as salts derived from non-toxic organic acids such as aliphatic mono- and dicarboxylic acids, phenyl-substituted alkane acids, hydroxy alkane acids, alkane diacids, aromatic acids, aliphatic and aromatic sulfonic acids, etc. Such salts include sulfates, nitrates, phosphates, monohydrogenphosphates, dihydrogenphosphates, metaphosphates, pyrophosphatec, chlorides, bromides, iodides , acetates, trifluoroacetates, propionates, caprylates, isobutyrates, oxalates, malonates, succinates, suberates, sebacates, fumarates, maleates, mandelates, benzoates, chlorobenzoates, methylbenzoates, dinitrobenzoates, phthalates, benzenesulfonates, toluenesulfonates, phenylacetates, citrates, lactates, maleate tartrates, methanesulfonates and the like. Also contemplated are amino acid salts such as arginates and the like, gluconates galacturonates (see, for example, Berge, S. M. et al., "Pharmaceutical Salts", Journal of Pharmaceutical Science, 66: 1-19 (1977).

Salts formed by the addition of acid from the mentioned basic compounds are prepared by combining the free base with the required amount of acid in a conventional way.

Pharmaceutically compatible base addition salts are formed with a metal or amine, such as alkali metals, alkaline earth metals or organic amines. Examples of metals that have been used as cations are: sodium, potassium, magnesium, calcium and the like. Examples of suitable amines are: N,N'-dibenzylethylenediamine, chlorprocaine, choline, diethanolamine, dicyclohexylamine, ethylenediamine, N-methylglucamine and procaine, see for example 8rge, S. M. et al., "Pharmaceutical Salts", Journal of Pharmaceutical Science, 66: 1 -19 (1977).

Salts formed by the addition of a base from the mentioned acidic compounds are prepared by combining the free acid group with the required amount of base in a conventional way.

Some compounds of the present invention can exist in non-solvated form as well as in solvated form, including hydrate forms. In general, solvated forms including hydrates are equivalent to unsolvated forms and are intended to be encompassed by the present invention.

Some compounds of the present invention may have one or more chiral centers, and each center may have an R(D) or S(L) configuration. The present invention includes all enantiomeric and epimeric forms, as well as their mixtures.

The compounds of the present invention can be prepared and administered in a wide variety of oral and parenteral dosage forms. Thus, the compounds of the present invention can be administered by injection, which can be intravenous, intramuscular, intracutaneous, subcutaneous, intraduodenal or intraperitoneal. The compounds of the present invention can also be administered by inhalation, for example intranasally. Furthermore, the compounds of the present invention can be administered transdermally. It will be apparent to those skilled in the art that the following dosage forms may contain as an active ingredient either a compound of formula 1 or a corresponding pharmaceutically compatible salt of formula 1.

For the preparation of pharmaceutical preparations from the compounds of the present invention, pharmaceutically compatible excipients can be either solid or liquid. Solid forms of preparations include powders, tablets, pills, capsules, sachets, suppositories and dispersible granules. A solid excipient may be one or more substances that may also act as diluents, flavor enhancers, binders, preservatives, tablet disintegrants, or encapsulating agents.

The powder contains finely divided 400 excipients in the form of a finely divided active component.

In tablets, the active component is mixed with an auxiliary agent that has the necessary binding properties in a suitable ratio and the mixture is pressed into the desired shape and size.

Powders and tablets preferably contain from about five or ten to about seventy percent of the active substance. Suitable excipients are: magnesium carbonate, magnesium stearate, talc, sugar, lactose, pectin, dextrin, corn starch, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose, low-melting wax, cocoa butter, and the like. The term "preparation" includes the formulation of an active compound with an encapsulating agent as an auxiliary agent, forming a capsule with or without other auxiliary agents, which is surrounded by the auxiliary agent, so that the agent is an integral part of the capsule. Similarly, a bag is included in oriblets. Tablets, powders, capsules, pills, sachets and tablets can be used as solid dosage forms suitable for oral administration.

A low-melting wax, such as a mixture of glycerides of fatty acids or cocoa butter, is used for the preparation of suppositories, which are melted first, and the active component is homogeneously dispersed in it. Then the melted homogeneous mixture is transferred into suitable molds, left to cool until solidification.

Liquid form preparations include solutions, suspensions and emulsions, for example an aqueous solution or an aqueous propylene glycol solution. For parenteral injection, liquid preparations can be formulated in an aqueous polyethylene glycol solution.

Aqueous solutions suitable for oral use can be prepared by dissolving the active component in water and optionally adding suitable coloring and flavoring agents, stabilizers and thickeners.

Aqueous suspensions suitable for oral use can be prepared by dispersing the finely divided active component in water with a viscous material such as natural or artificial gums, resins, methylcellulose, sodium carboxymethylcellulose, and such good suspending agents.

Also included in the invention are solid preparations that are converted shortly before use into liquid forms for oral administration. Such liquid forms include solutions, suspensions and emulsions. In addition to the active component, these preparations may also contain coloring and flavoring agents, stabilizers, buffers, artificial and natural sweeteners, disintegrants, thickeners, solubilizers and the like.

The pharmaceutical preparations are preferably in dosage units. In such forms, the preparation is divided into dosage units, each of which contains an appropriate amount of active substance. A dosage unit may be a packaged preparation or a package that may contain multiple unit preparations, such as packaged tablets, capsules, and powders in vials or ampoules. The unit dosage form may also be a capsule, sachet, or capsule by itself, or a corresponding number of any packaging form may be present.

The amount of the active component in a unit dose of the preparation can range from 0.1 mg to 100 mg, preferably 0.5 mg to 100 mg, depending on the needs of the specific application and the availability of the active substance. The preparation may optionally contain other compatible therapeutic substances.

For therapeutic purposes as protease antagonists of retroviruses including HIV, or as agents for the treatment of AIDS, the compounds used in the pharmaceutical method of this invention are administered daily in an initial dose of about 0.01 mg to about 100 mg/kg. A daily dose range of about 0.2 mg to about 10 mg/kg is preferred. The dose, however, can be changed, depending on the patient's requirements, the severity of the condition being treated and the compound used. Determining the appropriate dose for a particular case is left to the expert. In general, treatment is initiated with lower doses that are less than the optimal dose of the compound. Then the dose is gradually increased until the optimal effect under the given conditions. If desired, the daily dose can be divided and given in portions during the day.

4.1. General synthesis of pyrone derivatives

Scheme I shown below illustrates the preparation of 6-substituted-3-substituted pyrones.

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A suitable base, such as lithium diisopropylamide or lithium bis(trimethylsilyl)amide, is added to the ketone (I) at -78 °C to -45 °C in ether or THF, and when deprotonation is complete, chlorotrimethylsilane (TMS- C1) at -78 °C to 0 °C, whereby silylenol ether II is formed. Alternatively, trimethylsilyltrifluoromethanesulfonate (TMS-OTf) and trimethylamine are added to compound I at 0 °C in dichloromethane solution, whereby intermediate II is formed. Compound II is then reacted with the corresponding substituted malonate by heating them solvent-free or in xylene at 130-160 °C, yielding product III.

For the purposes of the above and other syntheses of compounds from the presented invention, reactive functional groups present in the starting compounds, reaction intermediates or reaction products can be protected during chemical reactions by using protective groups that convert the reactive functional group into sufficiently inert for the reaction conditions. (See for example Protective Groups in Organic Synthesis, 2nd ed., T.W. Green and P.G. Wuts, John Wiley & Sons, New York, NY 1991) Thus, for example, the following protective groups suitable for reducing the reactivity of amino, hydroxyl and other groups of similar reactivity can be used : carboxylic acyl groups, such as fromyl, acetyl, trifluoroacetyl; alkoxycarbonyl groups, such as ethoxycarbonyl, t-butoxycarbonyl (BOC), β,β,β-trichloroethoxycarbonyl (TCEC), β-iodoethoxycarbonyl; aryloxycarbonyl groups such as benzyloxycarbonyl, β-methoxybenzyloxycarbonyl, phenoxycarbonyl; trialkylsilyl groups such as trimethylsilyl and t-butyldimethylsilyl (TBDMS); and groups such as trityl, tetrahydropyranyl, vinyloxycarbonyl. onetriphenylsulfenyl diphenylphosphinyl, β -toluenesulfonyl and benzyl. After completion of the reaction, protective groups can be removed by known procedures. For example, the BOC group can be removed by acidolysis, the trityl group by hydrogenolysis, TBDMS by the action of fluoride ions, and TCEC by the action of zinc.

Scheme II describes an alternative synthesis of pyrones with functional groups.

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Trimethylsilylenol ether II is reacted with malonyl dichloride in a dry solvent, such as ether or THF, at low temperature, preferably -78 °C to -35 °C. whereby the pyrone IV is formed, which is converted to the sulfur derivative V with the appropriate substituted p-toluenesulfonate, as described in U.S. Pat. Polenta 3,931,235 (1976). Alternatively, the thiotosylate reagent is prepared as described by M.G. Ranasinghe and P.L. Fuchs in Syn. Cotnin. 18(3): 227 (1988). The required thiols can be prepared from the corresponding phenols via the Newman-Kwart rearrangement (see, for example, H. Kwart and H. Omura, J. Am. Chem. Soc. 93: 7250 (1971); M. S. Newman and F. W. Hetzel, Org. Syn. Coll. . Voli. VI: 825 (1988); M. S. Newman and H. A. Karnes, J. Org. Chem. 31: 3980 (1966)) or from the corresponding iodobenzene via nucleophilic substitution with thiourea in the presence of nickel as a catalyst (K. Takagi , Chem. Letters, 1307 (1985)).

The synthesis of pyrones such as VIII is shown below in Scheme III. Substituent R6 in structure VII can be aryl, alkyl or substituted alkyl.

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The substituted β-ketoester VI is deprotonated with one equivalent of a suitable base, eg a metal hydride such as sodium hydride, in a suitable solvent eg ether or THF. A second equivalent of a stronger base is added to the malonate solution, eg an alkyllithium such as n-butyllithium or lithium diisopropylamide, forming a dianiline, which is then reacted with a suitable acylating agent, e.g. with amide at 0 to 25 °C and dione ester VII is formed. Compound VII can be cyclized to pyrone VIII in various ways, for example using a strong acid such as H2SO4, or CH3SO3H while heating the reaction mixture in a high boiling solvent such as xylene or using a small amount of base, preferably a sterically hindered base such as 1, 8-diazabicyclo[5.4.0]undeca-7-ene. If R3 = H, then pyrone VIII can be converted into derivatives as shown in Scheme II above.

Scheme IV describes the preparation of o-acyl analogues of pyrones

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A suitable base is added to the suffix, for example VIII, e.g. metal hydride or alkoxide in a suitable solvent, e.g. dioxane or ether, and the resulting anion reacts with acyl chloride or another agent for acylation, whereby the acyl derivative IX is formed.

Scheme V below illustrates the preparation of several 3-alkylpyrone derivatives.

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The pyrone intermediate VIII is coupled in an alcoholic medium, such as ethanol, with a suitable aldehyde and a suitable nucleophile such as HSR 9 or NH 2 R 9 in the presence of a mixture of an acid, such as acetic acid, and a base, such as piperidine. The resulting mixture is heated at 60 °C to 90 °C and pyrone X is formed, which is already among the preferred compounds of this invention. In case Y=S, X can alternatively be reduced by metal reaction by reduction in a solvent, for example using sodium in liquid ammonia or reduction with Raney-nickel in a solvent such as acetone, whereby the desired 3-alkylated pyrones XI are formed.

Scheme VI summarizes the synthesis of some specific 3-alkylpyrone derivatives.

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Nitration of pyrone VIII is carried out with nitric acid, preferably with fuming nitric acid in acidic solution, for example as described in U.S. Pat. Patent 3,206,476 (1965). Reduction of nitropyrone XII with zinc produces aminopyrone XIII. Intermediate XIII can be converted into a large number of derivatives. For example, a suitably substituted aldehyde can be added to compound XIII in the presence of a reducing agent such as sodium borohydride or, preferably, sodium cyanoborohydride, whereby the N-alkylated analog of compound XIV is formed. Acylation of compound XIII can be achieved through several synthetic procedures: 1. by treatment with sodium hydride followed by addition of a mixture of a suitable carbocyclic acid, N-methylmorpholine and a suitable condensing agent, such as 1-(3-di-methylaminopropyl)-3- ethylcarbodiimide, at a suitable temperature, e.g. -35 °C to 0 °C; 2. by reaction with a suitable acid chloride or other acylating agent in the presence of a base such as triethylamine and 4-dimethylaminopyridine; or 3. deprotonation with sodium hydride, followed by reaction with a suitable acid chloride in the presence of an excess of an amine base, usually triethylamine, at an elevated temperature, eg 40-60 °C. Ureas, such as compound XVI, can be prepared from aminopyrone XIII by reaction with a suitable isocyanate and a base, for example N-methylmorpholine in an inert solvent such as ethyl acetate.

Scheme VI shows an alternative approach for the preparation of C-6 substituted analogs.

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2 equivalents of a strong base are added to 3-Methylpyrone XVIII, e.g. sodium amide in liquid ammonia or lithium diisopropylamide in THF solution, followed by reaction with one of a large number of different electrophiles, e.g. alkyl halides, acylating agents, etc., to form pyrone XVIII (see M.P. Wachter and T.M. Harris, Tetrahedrom 26: 1685 (1970)). Alternatively, bromination of XVIII under free radical-generating conditions, for example using N-bromosuccinimide (NBS) in the presence of radical-inducing light, produces intermediate XIX, which is converted to amine XX as described by Jones et al. Tetrahedron Letters, 30: 3217 (1989), further to alcohol XXI, as described by R. Bacardit et al, J. Heterocyclic Chem. 19: 157 (1982), and was finally converted to sulfide XXII as described by R. Bacardit et al, J. Heterocyclic Chem. 26: 1205 (1989). Amino and hydroxyl groups in structures XX and XXI can be further converted into derivatives by known methods, for example by alkylation, acylation, etc.

The synthesis of several 4-substituted pyrone derivatives is shown in Scheme VIII.

[image]

Pyrone VIII is activated, for example, by tosylation to compound XXIII using p-toluenesulfonyl chloride (TsCl) in pyridine. A suitable sulfur nucleophile is then added to the tosylate (see A. M. Bittencourt et al, Tetrahedron, 27': 1043 (1971), to form sulfide XXIV. Similarly, using a brominating agent such as phosphorus tribromide/dimethylformamide (DMF), pyrone VIII converted to the 4-bromo analogue XXV.

Substitution of bromine in XXV with azide, followed by reduction (eg preferred hydrogenation with palladium/triaryl-phosphine as a catalyst and a suitable solvent) results in the 4-amino derivative XXIX. Further conversion of the amine moiety of compound XXIX was accomplished as shown in Scheme VI.

Alternatively, 4-bromopyrone XXV can be reacted with a palladium/triarylphosphine catalyst and methanol in a carbon monoxide atmosphere, whereby ester XXVI is formed. The ester can be hydrolyzed, for example with an acid solution at 0-25 °C to the corresponding carboxylic acid, or reduced for example with a hydride reagent, such as lithium aluminum hydride, in THF or ether at 0-25 °C, whereby an alcohol is formed ( XXVII).

Scheme IX illustrates the preparation of the 2H-thiopyron-2-one derivative.

[image]

A suitably substituted β-mercapro-acrylate, example XXX, is condensed with the desired malonyl dichloride in an inert solvent, e.g. toluene at a temperature between 0 °C and the boiling point of the reaction solvent, whereby thiopyron-2-one XXXI is formed. Thiopyronone XXXI can be converted to derivative XXXII under suitable conditions, for example by hydrolysis followed by decarboxylation (eg, see F.K. Splinter and H. Arold, J. Prakt. Cheni., 38: 3-4 142-6). Thiopyrones XXXII (R3 = H) can be converted to their substituted derivatives using the procedures for the preparation of pyrone derivatives of Schemes II, X and VI.

Suitable pyrone protecting groups, e.g. XVII as well as their analogs having S instead of O in the 1-position of the pyrone ring can be thilated, i.e. The carbonyl group in the 2-position of the heterocyclic ring can be replaced by a thiocarbonyl (C=O → C=S) using standard group modification techniques, for example using a thiation reagent such as Lavvessen's reagent under suitable conditions (see Montash. Chem., 115: 769 ( 1984) and Chem, Rev. 84: 17 (1984)).

4.2. General procedures Preparation of pyrones with functional groups

Method A: Synthesis by reaction of silylenol-ether and 2-substituted esters of propane-diacid

i) Preparation of trimethylsilylenol ether

To a solution of the appropriate ketone (10 mmol, 1 equivalent) in dry tetrahydrofuran (100 mL) was added lithium hexamethyldisilazide (11 mmol, 1.1 equivalent) at -78 °C. The reaction mixture was stirred for 1 hour at -78 °C, and for 0.5 hours at -35 °C. Thimethylchlorosilane was then added dropwise at -78 °C, and the reaction mixture was stirred for 1 hour at -78 °C, and then for 0.5 hours at 0 °C. The reaction was quenched by the addition of saturated sodium bicarbonate solution and the reaction mixture was extracted with ethyl acetate (300 mL). The ethyl acetate layer was washed with saturated sodium bicarbonate solution, sodium chloride solution and dried over anhydrous sodium sulfate. The ethyl acetate solution was concentrated under reduced pressure and the isolated material was dried under vacuum for 1 hour and used without further purification.

ii) Condensation of trimethylsilylenol ether and dialkyl ester of 2-substituted propane diacid

Crude trimethylsilylenol ether (11 mmol, 1.1 equiv), which was prepared as described above, was combined with 2-substituted propane diacid (10 mmol, 1.0 equiv), and the resulting reaction mixture was heated at 150 °C overnight. , with a constant flow of nitrogen through the reaction mixture. The reaction mixture was cooled to room temperature and the product was purified by silica gel chromatography. Elution with 10-15% ethyl acetate/hexane removed the unreacted starting material and other impurities, and elution with 30-50% ethyl acetate/5% methylene chloride/hexane yielded the desired pyrones in a yield of 20-75%.

Method B: Sulphenylation of 6-aryl-4-hydroxy-2H-pyran-2-one

i) Preparation of 6-aryl-4-hydroxy-2-pyran

Trimethylsilylenol ether (20 mmol, 1 equivalent), which was prepared as described in Method A (or obtained commercially), was added to anhydrous ether and cooled to -78 °C to -40 °C. To this was added malonyl dichloride (30-40 mmol, 1.5-2 equivalents). The reaction mixture was gradually warmed to room temperature and stirred overnight. The resulting solid was filtered and washed with anhydrous ether.

ii) Sulphenylation of 6-aryl-4-hydroxy-2H-pyran-2-one

6-Aryl-4-hydroxy-2-pyran (1.62 mmol, 1 equivalent), which was prepared as described above, was dissolved in ethanol. To this solution was added 1 M sodium hydroxide (1.72 mL, 1.04 equivalents) or 2 equivalents of triethylamine, followed by addition of the appropriate thiolsulfonate (1.72 mL, 1.04 equivalents). The reaction mixture was heated to reflux overnight. The solvents were removed, acidified with 1 M HCl and the product was extracted with ethyl acetate. After evaporation of the solvent, the crude product was purified by chromatography (silica gel 200-400 mesh) using 30-50% ethyl acetate in hexane to give the desired product. Utilization: 40-80%.

Method C: Preparation of (6-aryl-4-hydroxy-2-oxo-2H-pyran-3-yl)-arylthiomethane

To 6-Aryl-4-hydroxy-2H-pyran-2-one (2.16 mmol, 1 equiv.) in 10 mL of ethylanol was added the corresponding aldehyde (2.37 mmol, 1.1 equiv.), piperidine (0.50 mL) and acetic acid (0.50 mL ). The reaction mixture was maintained at 80 °C for 24 hours. Ethanol was evaporated, acidified with 1 M HCl, and the residue was purified by chromatography (silica gel 200-400 mesh), whereby the desired product was obtained in 30-60% yield.

Method D: Preparation of 6-aryl-3-alkylamino-4-hydroxy-2H-pn 2-one

i) 6-Aryl-4-hydroxy-3-nitro-2H-pyran-2-one

The method used is a modified procedure described in U.S. Pat. Patent 3,206,476 (1965) for nitration and reduction. Fuming nitric acid (0.222 mL) was added to a suspension of 6-aryl-4-hydroxy-2H-pyran-2-one (2.65 mmol) in acetic acid (2.77 mL) at room temperature. After stirring for 5 minutes, the reaction mixture was cooled to 0°C and the product was filtered. The product was purified by recrystallization from boiling acetic acid. 1H NMR (250 Hz, d-TFA) δ 7.02 (s, 1H), 7.65 (s, 3H), 7.99 (m, 2H).

ii) 3-Amino-6-aryl-4-hydroxy-2H-pyran-2-one

To a suspension of 6-Aryl-4-hydroxy-3-nitro-2H-pyran-2-one (10.5 mmol, 1 equivalent) in acetic acid (15 mL) in concentrated HCl (7.34 mL) was added mossy tin (20.6 mmol, 1.96 equivalents). The mixture was then heated to reflux, resulting in a homogeneous mixture. The reaction mixture was refluxed for 7 minutes and cooled in an ice bath. Concentrated HCl was added, precipitating 3-amino-4-hydroxy-2H-pyran-2-one hydrochloride, which was collected and dried. 1H NMR (250 Hz, D2O) δ 6.74 (s, 1H), 7.53 (m, 3H), 7.84 (m, 2H).

iii) 3-Alkylamino-6-aryl-4-hydroxy-2H-pyran-2-one

To a solution of 3-amino-6-aryl-4-hydroxy-2H-pyran-2-one hydrochloride (2 mmol, 1 equivalent) in dimethylformamide containing 1% acetic acid (20 mL) in concentrated HCl (7.34 mL) was added the aldehyde (2.1-4.2 mmol, 1.05-2.1 equivalents), followed by sodium cyanoborohydride (2.1-4.2 mmol, 1.05-2.1 equivalents). The reaction was stirred for 5 minutes, "quenched" with water and concentrated in vacuo. The oily residue was dissolved in 100 mL of ethyl acetate, washed with water, then with saturated sodium chloride solution, and dried over anhydrous magnesium sulfate. After evaporating the solvent in a vacuum, the crude product was purified by column chromatography (silica gel 230-400 mesh) or recrystallized, whereby the desired product was obtained.

Method E: 3-Acylamino-6-aryl-4-hydroxy-2H-pyrari-2-one

The following procedures were used for the amidation of 3-amino-6-aryl-4-hydroxy-2H-pyran-2-one.

a) 60% sodium hydride (0.92 mmol, 1.1 equivalent) was added to a solution of 3-amino-6-aryl-4-hydroxy-2H-pyran-2-one hydrochloride (0.84 mmol, 1.0 equivalent) in THF (10 mL). after which it was stirred for 30 minutes at room temperature. In a separate flask corresponding to carboxylic acid (1.67 mmol, 2 equivalents) in THF (20 mL) at -20 °C was added N-methylmorpholine (0.92 mmol, 1.1 equivalents), followed by 1-(3-dimethylaminopropyl)-3-ethyl -carbodiimide hydrochloride (0.92 mmol, 1.1 equivalents). The reaction mixture was stirred for 1 hour at -20 °C.

This solution was added to the above solution of 3-amino-6-aryl-4-hydroxy-2H-pyran-2-one hydrochloride, and then more N-methylmorpholine (0.918 mmol, 1.1 equivalent) was added. The reaction mixture was stirred at room temperature overnight. The reaction was "quenched" by the addition of sodium chloride solution, and was diluted with ethyl acetate. The organic layer was washed with 1M HCl, then with water, and saturated sodium chloride solution, and dried over anhydrous magnesium sulfate. After evaporation of the solvent in vacuo, the crude product was purified by column chromatography (silica gel 230-400 mesh), whereby the desired product was obtained.

b) Triethylamine (3.3 mmol, 4.0 equivalents) was added to a suspension of 3-amino-6-aryl-4-hydroxy-2H-pyran-2-one monohydrochloride (0.83 mmol, 1.0 equivalent) in methylene chloride (8 mL), and then a catalytic amount of 4-dimethylaminopyridine (0.08 mmol, 0.1 equivalent) and the corresponding acid chloride (0.92 mmol, 1.1 equivalent). The reaction was stirred for 6 hours at room temperature. The reaction was "quenched" with 1 M hydrochloric acid and diluted with methylene chloride. The organic layer was washed with water and saturated sodium chloride solution and dried over anhydrous magnesium sulfate. After evaporation of the solvent in vacuo, the crude product was recrystallized from boiling acetic acid.

c) 60% sodium hydride (0.69 mmol, 1.1 equivalent) was added to a solution of 3-amino-6-aryl-4-hydroxy-2H-pyran-2-one monohydrochloride (0.63 mmol, 1.0 equivalent) in tetrahydrofuran (6 mL). at 0 °C. The resulting mixture was stirred at room temperature for 15 minutes.

The corresponding acid chloride (0.69 mmol, 1.1 equivalent) was added to the reaction mixture. The reaction mixture was heated at 50 °C for 1 hour, and overnight at room temperature. The reaction was "quenched" with 1M hydrochloric acid and diluted with ethyl acetate. The organic layer was washed with saturated sodium chloride solution and dried over anhydrous magnesium sulfate.

After evaporation of the solvent in vacuo, the crude product was recrystallized from boiling nitromethane, whereby the pure product was obtained.

Method F: Preparation of 3-Alkyl-6-aryl-4-hydroxy-2H-pyran-2-one

(6-Aryl-4-hydroxy-2-oxo-2H-pyran-3-yl)arylthiomethanes were prepared as described in Method C. Raney-nickel (Grave 3100) was heated in acetone to reflux for 45 min and acetone was replaced by ethanol (20 mL). (4-Hydroxy-6-substituted-2-oxo-2H-pyran-3-yl)arylthiomethane (1.0 mmol, 1 equiv) was added and the resulting mixture was heated to reflux overnight. The mixture was filtered through celite and washed with hot ethanol. The filtrate is concentrated in vacuo to give pure products.

Method G: Preparation of 4-acyloyl ester of 4-hydroxy-6-aryl (or arylalkyl)-thio-6-aryl-2H-pyran-2-one

4-Hydroxy-3-aryl(or arylalkyl)thio-6-aryl-2H-pyran-2-one (3 mmol, 1 equivalent) was dissolved in 20 mL of tetrahydrofuran and cooled to 0 °C. Sodium hydride (3.3 mmol, 1.1 equivalent) was slowly added to this mixture and the resulting mixture was heated for 15 minutes at room temperature. The appropriate acid chloride (6 mmol, 2 equivalents) was added dropwise and the reaction was stirred at room temperature overnight. The reaction was "quenched" with saturated sodium chloride solution and was diluted with 100 mL of ethyl acetate. The combined organic layers were washed with sodium bicarbonate solution, sodium chloride solution and dried over anhydrous sodium sulfate. After evaporation of the solvent, the syrupy product was purified by column chromatography (silica gel 230-400 mesh) using 10% ethyl acetate in hexane as eluent, whereby the phenol ester was obtained in a yield of 70-85%.

4.3. Preparation of starting materials

Examples A-E: Preparation of propane-diacid

The following key intermediates were synthesized according to the procedure described in Complus rendus 255:2611 (1962).

Example A

Diethyl [(phenylmethyl)thio]propanedioate: boiling point 160-162 °C/6 mmHg; 1H NMR (250 MHz, DMSO-d6) δ 1.18 (t, 6H), 3.93 (s, 2H), 4.13 (q, 1H), 4.44 (s, 1H), 7.31 (m, 7H).

Example B

Diethyl [(2-naphthalenylmethyl)thio]propane diacids

Crude product purification by chromatography on silica gel (silica gel 230-400 mesh). 1H NMR (250 MHz, DMSO-d6) δ 3.65 (s, 6H), 4.10 (s, 2H), 4.55 (s, 1H), 7.15 (m, 3H), 7.51 (m, 4H).

Example C

Diethyl ester [(3-phenylpropyl)thio]propanedioate

Boiling point 185-190 °C/1 mmHg; 1H NMR (400 MHz, DMSO-d6) δ 1.17 (t, 6H), 1.82 (m, 2H), 2.65 (q, 4H), 4.14 (q, 4H), 4.62 (s, IH), 7.23 (m, 5H).

Example D

[(2-Naphthalenylniethyl)thio]propanedioate diethyl ester

Crude product purification by chromatography on silica gel (silica gel 230-400 mesh). 1H NMR (250 MHz, DMSO-d6) δ 1.09 (t, 6H), 4.12 (q, 4H), 5.27 (s, 1H), 7.58 (m, 3H), 7.90 (m, 3H), 8.80 (s, THEM).

Example E

Diethyl ester [(2-phenylethyl)thiopropanedioate

Boiling point 160-165 °C/1 mmHg; *H NMR (400 MHz, DMSO-d6) δ 1.19 (t, 6H), 2.89 (m, 2H), 4.16 (q, 4H), 4.68 (s, 1H), 7.25 (m, 5H). ;Examples F-M: ;Preparation of p-toluenethiosulfonate ;The following key intermediates were synthesized according to the procedure described in U.S. Pat. Patent 3,931,235(1976). Example F 2-Phenoxyethyl-p-toluenethiosulfonate 1H NMR (400 MHz, DMSO-d6) δ 2.41 (s, 3H), 3.41 (t, 2H), 4.13 (t, 2H), 6.83 (d, 2H) , 6.94 (t, 1H), 7.27 (t, 2H), 7.48 (d, 2H), 7.85 (d, 2H). Example G 3-Phenyl-2-propaneyl-p-toluenethiosulphonate 1H NMR (400 MHz, DMSO-d6) δ 2.32 (s, 3H), 3.93 (d, 2H), 6.00 (dt, IH), 6.58 ( d, 1H), 7.29 (m, 5H), 7.38 (d, 2H), 7.81 (d, 2H). ;Example H ;2-[2-Methoxyphenyl]ethyl-p-toluenethiosulphonate ;1H NMR (400 MHz, DMSO-d6) δ 2.43 (s, 3H), 2.80 (t, 2H), 3.19 (t, 2H), 3.75 (s, 3H), 6.83 (t, 1H), 6.93 (d, 1H), 7.02 (d, 1H), 7.21 (t, 1H), 7.49 (d, 2H), 7.81 (d, 2H). Example I 4-Phenyl-p-toluenethiosulfonate: 1H NMR (400 MHz, DMSO-d6) δ 1.53 (m, 4H), 2.43 (s, 3H), 2.50 (t, 2H), 3.03 (t, 2H) ), 7.12 (d, 1H), 7.18 (d, 2H), 7.25 (t, 2H), 7.45 (d, 2H), 7.80 (d, 2H). Example J 2-[3-Methoxyphenyl]ethyl-p-toluenethiosulfonate 1H NMR (400 MHz, DMSO-d6) δ 2.43 (s, 3H), 2.79 (t, 2H), 3.25 (t, 2H), 3.73 (s, 3H). 6.73 (m, 3H), 7.19 (m, 1H), 7.49 (d, 2H), 7.83 (d, 2H). Example K 2-[4-Methoxyphenyl]ethyl-p-toluenethiosulfonate 1H NMR (400 MHz, DMSO-d6) δ 2.50 (s, 3H), 2.76 (t, 2H), 3.21 (t, 2H), 3.71 (s, 3H), 6.83 (t, 1H), 7.03 (d, 2H), 7.50 (t, 2H), 7.82 (d, 2H). Example L 2-(2-chlorophenyl)ethyl-p-toluenethiosulfonate 1H NMR (400 MHz, DMSO-d6) δ 2.41 (s, 3H), 2.86 (t, 2H), 3.28 (t, 2H), 7.22 (m, 4H), 7.49 (d, 2H), 7.83 (d, 2H). ;Example M ;[4-(Phenylmethoxy)phenyl]methyl-p-toluenethiosulfonate ;1H NMR (400 MHz, DMSO-d6) δ 2.41 (s, 3H), 4.28 (s, 2H), 5.06 (s, 2H), 6.87 (d, 2H), 7.13 (d, 2H), 7.37 (m, 7H), 7.72 (d, 2H). ;Example N ;6-(3-chlorophenyl)-4-hydroxy-2H-pyran-2-one ;A mixture of 60% NaH (0.790 g, 19.7 mmol) in THF (50 mL) under a nitrogen atmosphere was cooled to 0 °C and ethyl acetoacetate (2.52 mL, 19.7 mmol) was added. n-butyllithium (12.3 mL, 19.7 mmol) was added to the resulting solution and stirred for 20 minutes at 0 °C, during which an orange solution was formed to which a solution of 3-chloro-N-methoxy-N-methylbezamide (2.50 g, 15.15 mmol) in THF (5.0 mL). The mixture was allowed to warm to room temperature and stirred for 14 hours before 2.0 M HCl was added. The product was extracted with ethyl acetate (3 x 50 mL), the layers were combined, dried over Na2SO4, and the solvent was evaporated under vacuum. To the residue was added conc. H2SO4, the resulting mixture was stirred for 18 h at room temperature and then diluted with water (200 mL). The product was then extracted with ethyl acetate (3 x 100 mL) which ensured complete isolation of the solids. The layers were combined and diluted with acetone, resulting in a homogeneous solution that was dried over Na2SO4. The solid was recrystallized from acetone/hexane to give the title compound (1.33 g, mp 254-256 °C) 1H NMR (400 MHz, DMSO-d6) δ 11.957 (broad s, IH), 7.889 (t, JH, J=1.5 Hz), 7.839-7.813 (m, IH), 7.598-7.524 (m, 2H), 7.876 (d, IH, J=2 Hz), 5.450 (d, IH, J=2 Hz). ;Example O ;6-(4-Chlorophenyl)-4-hydroxy-2H-pyran-2-one ;The title compound (1.56 g, melting point 247-249 °C) was prepared in a similar manner as shown in the preparation of Example N using: 60% NaH (0.904 g, 22.6 mmol), THF (50 mL), ethyl acetoacetate (3.00 g, 22.6 mmol), lithium diisopropylamine in THF (39.8 mL, 24 mmol), 4-chloro-N- methoxy-N-methylbenzamide (3.73 g, 22.6 mmol) and 90% H2SO4 (20 mL). ;1H NMR (300 MHz, DMSO-d6) δ 11.950 (broad with ,1H), 7.878 (d, IH, J=9 Hz), 7.584 (d, IH, J=9 Hz), 6.812 (d, IH, J=2 Hz), 5.409 (d, IH, J=2 Hz). ;Example P ;(Cyclopropylmethyl)-p-toluenethiosulfonate ;To a solution of methylcyclopropyl bromide (4.00 g, 29.6 mmol) in ethanol (22.0 mL) was added sodium thiotosylate (10.0 g, 44.4 mmol) and the mixture was heated for 10 hours at 90 ° C. The mixture was then poured into a 1:1 mixture of H2O (50.0 mL) and diethyl ether (50.0 mL). The layers were separated, and the organic layer was washed with sodium chloride solution (50.0 mL). The organic layer was dried over MgSO2 and concentrated in vacuo to give the title compound as a solid (5.2 g, mp 46-48 °C). 1H NMR (400 MHz, CDCl3) δ 7.816 (d, 2H, J=8.8 Hz), 7.308 (d, 2H, J=8.8 Hz), 2.945 (d, 2H, J=7.6 Hz), 2.451 (s, 3H ), 1.010-0.933 (m, 1H), 0.592-0.545 (m, 2H), 0.236-0.197 (m, 2H). ;Example Q ;Methyl-[4-(1-oxoethyl)phenoxy] acetate ;Methylbromoacetate (7.26 mL) was added to a mixture of 4-hydroxypropiophenone (10.0 g, 60.24 mmol), CsCO3 (21.6 g, 66.3 mmol) and acetone (150.0 mL). , 78.3 mmol) in a nitrogen atmosphere, and the mixture was heated to reflux for 4 h. The mixture was then allowed to warm to room temperature, diluted with water (150 mL), and extracted with CH2Cl2 (2 x 300 mL). The organic layers were combined, dried over Na2SO4, and the solvent was removed in vacuo to give the title compound (12.75 g, mp 64-66 °C). 1H NMR (400MHz, DMSO-d6) δ 9.35 (d. 2H, J=8.9 Hz), 7.040 (d, 2H, J=8.9 Hz), 4.920 (s, 2H), 3.715 (s, 3H), 2.981 ( q, 2H, J=7.2 Hz), 1.071 (t, 3H, J=7.2 Hz). ;4.4. Preparation of specific pyrone derivatives; Example 1 6-(3-Chlorophenyl)-4-hydroxy-3-[(phenylmethyl)thio]-2H-pyran-2-one; Following Method A, a solution of 3'-coracetophenone (1.50 g, 11.6 mmol) and THF (10.0 mL) was cooled to -78 °C (N2 atmosphere) and a 1.0 M solution of lithium hexamethyldisilazide (12.5 mL, 12.5 mmol) in THF was added. The solution was heated to 0 °C and stirred for 15 minutes, then trimethylsilyl chloride (1.47 mL, 11.6 mmol) was added. The reaction mixture was left with stirring for 0.5 hours (room temperature), and then it was poured into a mixture of diethyl ether (50 mL) and saturated aqueous NaHCO3 solution (20 mL). The layers were separated, and the organic layer was washed with a 1:1 mixture of sodium chloride solution and NaHCO3 (20 mL). The ethereal solution was then dried over Na 2 SO 4 , and the solvent was removed in vacuo. The resulting silylenol ether was transferred to a flask containing diethyl 2-(thiobenzyl)propane-1,3-dioate (1.63 g, 5.80 mmol), and the resulting mixture was heated for 16 h at 160 °C and allowed to cool. to room temperature, where it was diluted with diethyl ether (20 mL) and extracted with saturated Na2CO3 solution (3 x 20 mL). The aqueous layer is acidified conc. HCl to pH 0, and extracted with ethyl acetate (3 x 100 mL). The organic layers were combined, dried over Na2SO4, and the solvent was removed in vacuo. The resulting residue was purified by chromatography (SiO2 230-400 mesh, 100% CH2Cl2 to 1% MeOH/CH2Cl2), whereby a solid was obtained which was recrystallized from acetone/hexane, yielding 0.436 g of product (m.p. 136-137 °C). 1H NMR (400 MHz, DMSO-d6) δ 11.950 (broad, IH), 7.814 (s, IH), 7.761 (d, IH, J=7.5 Hz), 7.616-7.534 (m, 2H), 7.271-7.185 (m, 5H), 6.811 (s, 1H), 4.023 (s, 2H). Example 2: 6-(2-chlorophenyl)-4-hydroxy-3-[(phenylmethyl)thio]-2H-pyran-2-one; The title compound (0.210 g, mp 99-101 °C) was prepared according to method A, using 2'-chloroacetophenone (1.50 mL, 11.6 mmol), 1.87 M potassium hexamethylsilazide (6.80 mL, 12.7 mmol) trimethylsilyl chloride (1.47 mL , 11.6 mmol), THF (10.0 mL), and diethyl 2-(thiobenzyl)propane-1,3-dioate (1.3 g, 4.63 mmol). 1H NMR (400 MHz, DMSO-d6) δ 12.153 (broad s, IH ), 7.639 (t, 2H, J=9 Hz), 7.572-7.747 (m, 2H), 7.276-7.206 (m, 5H), 6.558 (s, IH), 4.029 (s, 2H). ; Example 3 ; 6-(3,4-Dichlorophenyl)-4-hydroxy-3-[(phenylmethyl)thio]-2H-pyran-2-one; Title compound (0.201 g, m.p. 185-186 °C) was prepared according to method A, using 3',4'-dichloroacetophenone (1.5 g, 7.9 mmol), 1.0 M lithium hexamethylsilazide (8.7 mL, 8.69 mmol) trimethylsilyl chloride (1.0 mL, 7.9 mmol) , THF (10.0 mL), and diethyl 2-(thiobenzyl)propane-1,3-dioate (0.89 g, 3.2 mmol). 1H NMR (400 MHz, DMSO-d6) δ 12.000 (broad s, IH), 8.018 (s, IH), 7.784 (s, 2H), 7.265-7.179 (m, 5H), 6.839 (s, IH), 4.017 (s, 2H). ;Example 4 ;4-Hydroxy-6-(3-methoxyphenyl)-3-[(phenylmethyl)thio]-2H-pyran-2-one ;The title compound (0.400 g, melting point 146-147 °C) was prepared according to the method A, using 3'-methoxyacetophenone (1.5 mL, 10.9 mmol), 1.0 M potassium hexamethylsilazide (6.41 mL, 12.0 mmol), trimethylsilyl chloride (1.38 mL, 10.9 mmol), THF (10.0 mL), and diethyl 2-(thiobenzyl )propane-1,3-dioate (1.23 g, 4.36 mmol). 1H NMR (400 MHz, DMSO-d6) δ 11.880 (broad s, IH), 7.445 (t, IH, J=8 Hz), 7.370 (d, j=8 Hz, 7.286-7.094 (m, 6H), 7.109 (m, 1H), 6.770 (s, 1H), 4.020 (s, 2H), 3.83l (s, 3H). ; Example 5 ; 4-Hydroxy-3-[(phenyleneethyl)thio]-6-(3, 4,5-trimethoxyphenyl)-2H-pyran-2-one; The title compound (0.385 g, mp 156-157 °C) was prepared according to method A, using 3',4',5'-trimethoxyacetophenone (2.0 g, 9.5 mmol), potassium hexamethylsilazid (5.6 mL, 10.45 mmol), trimethylsilyl chloride (1.2 mL, 9.5 mmol), THF (15 mL), and diethyl 2-(thiobenzyl)propane-1,3-dioate (1.07 g, 3.80 mmol) ).1H NMR (400 MHz, DMSO-d6) δ 11.778 (broad s, IH), 7.265-7.181 (m, 5H), 7.054 (s, 2H), 6.792 (s, IH), 3.997 (s, 2H) , 3.861 (s, 6H), 3.727 (s, 3H).; Example 6; 6-(3-Chlorophenyl)-4-hydroxy-3-[(2-phenylethyl)thio]-2H-pyran-2-one; The title compound (0.138 g, mp 125-127 °C) was prepared according to method B, using 6-(3-chlorophenyl)-4-hydroxy-2H-pyran-2-one (0.250 mg, 1.10 mmol), phenethyl-p -toluene-thiosulfonate (0.43 g, 1.46 mmol), triethylamine (0.35 mL), 2.5 mmol), and ethanol (5.0 mL). 1H NMR (400 MHz, CDCl3) δ 7.838 (t, IH, J=1.5 Hz), 7.710 (d, IH, J=8 Hz), 7.530 (broad s, IH), 7.475-7.392 (m, 2H), 7.308-7.260 (m, 2H), 7.207-7.171 (m, 3H), 6.604 (s, 1H), 3.125 (t, 2H, J=7 Hz), 2.897 (t, 2H, J=7 Hz). Example 7 6-(4-Chlorophenyl)-4-Hydroxy-3-[(2-phenylethyl)thio]-2H-pyran-2-one The title compound (0.242 g, melting point 161-163 °C) was prepared according to method B, using 6-(4-chlorophenyl)-4-hydroxy-2H-pyran-2-one (0.250 mg, 1.12 mmol), phenethyl p-toluenethiosulfonate (0.390 g, 1.35 mmol), triethylamine (0.31 mL) , 2.24 mmol), and ethanol (10.0 mL). 1H NMR (400 MHz, CDCl3) δ 12.085 (broad s, IH), 7.827 (d, 2H, J=9 Hz), 7.605 (d, 2H, J=9 Hz), 7.259-7.142 (m, 5H), 6.830 (s, IH), 3.017 (t, 2H, J=7.5 Hz), 2.785 (t, 2H, J=7.5 Hz). ;Example 8 ;4-Hydroxy-6-phenyl-3-[(phenylmethyl)thio]-2H-pyran-2-one ;The title compound was prepared according to method A, using 1-phenyl-1-(trimethylsilyloxy)ethylene (1.0 g, 5.19 mmol) of diethyl [(phenylmethyl)thio]propanedioate (0.977 g, 3.46 mmol). Melting point 155-160 °C; 1H NMR (250 MHz, DMSO-d6) δ 4.00 (s, 2H), 6.74 (s, 1H), 7.23 (m, 5H), 7.53 (m, 3H), 7.78 (m, 2H). ;Example 9 ;4-Hydroxy-6-phenyl-3-[(phenylmethyl)amino]-2H-pyran-2-one ;The title compound was prepared according to method D, using 3-amino-4-hydroxy-6-phenyl- 2H-pyran-2-one hydrochloride (0.500 g, 2.08 mmol), 1% acetic acid in dimethylformamide (20 mL), benzaldehyde (0.233 mL, 2.29 mmol) and sodium cyanoborohydride (0.144 g, 2.29 mmol). Melting point 205 °C with decomposition; 1H NMR (250 MHz, DMSO-d6) δ 4.37 (s, 2H), 6.56 (s, 1H), 7.27 (m, 5H), 7.45 (m, 3H), 7.67 (m, 2H). Example 10 N-(1,1-Dimethylethyl)-N'-(4-hydroxy-2-oxo-6-phenyl-2H-pyran-3-yl-N'-(phenylmethyl)urea: In suspension 4 -hydroxy-6-phenyl-3-(phenylmethyl)amino-2H-pyran-2-one monohydrochloride (1.53 mL) in ethyl acetate was added N-methylmorpholine (2.0 mL) and tert-butyl isocyanate (2.0 mL). The reaction mixture was left for 2.5 hours with stirring, and was diluted with ethyl 1-acetate. The organic layer was washed with 5% citric acid and saturated sodium chloride solution, and was dried over anhydrous magnesium sulfate. After evaporation of the solvent in vacuo, the crude product was purified by column chromatography (silica gel 240-400 mesh) using 5% methanol in dichloromethane as eluent 1H NMR (250 MHz, DMSO-d6) δ 1.24 (s, 9H), 4.47 (dd, 2H), 5.45 (broad s, IH ), 7.23 (m, 5H), 7.51 (m, 3H), 7.75 (m, 2H). ; Example 11 ; 4-Hydroxy-3-[(2-naphthalenylmethyl)thio]-6-phenyl-2H-pyran- 2-one ; The title compound was prepared according to method A, using 1-phenyl-1-(trimethylsilyloxy)ethylene (0.475 g, 2.46 mmol) and dimethyl [(2-naphthalenylmethyl)thio]propanedioate a (0.500 g, 1.64 mmol). When melting >250 °C, the compound breaks down; 1H NMR (250 MHz, DMSO-d6) δ 4.06 (s, 2H), 6.47 (s, 1H), 7.46 (m, 6H), 7.78 (m, 6H). ;Example 12 ;4-Hydroxy-3-[(2-naphthalenyl)thio]-6-phenyl-2H-pyran-2-one ;The title compound was prepared according to method A, using 1-phenyl-1-(trimethylsilyloxy)ethylene (1.33 g, 6.90 mmol) of diethyl [(2-naphthalenyl)thio]propanedioate (2.00 g, 6.29 mmol). Melting point 246 °C with decomposition; 1H NMR (250 MHz, DMSO-d6) δ 6.95 (s, 1H), 7.38 (m, 3H), 7.56 (m, 4H), 7.85 (m, 5H). Example 13 4-Hydroxy-3-[(phenylmethyl)thio]-6-(2,4,6-trimethylphenyl)-2H-pyran-2-one The title compound was prepared according to method A, using 2',4 ',6'-trimethylacetophenone (1.86 g, 11.5 mmol) lithium bis(trimethylsilyl)amide (2.11 g, 12.65 mmol) chlorotrimethylsilane (1.60 mL, 12.65 mmol), THF (127 mL) and diethyl [(phenylmethyl)thio]propanedioate (2.95 g, 10.4 mmol). Melting point 134-136 °C; 1H NMR (250 MHz, DMSO-d6) δ 2.11 (s, 6H), 2.26 (s, 3H), 3.98 (s, 2H), 6.03 (s, 1H), 6.96 (s, 2H), 7.25 (m, 5H), 11.85 (wide s, IH). ; Example 14 ; 4-Hydroxy-6-[4-[2-(4-morphoIinyl)ethoxy]phenyl]-3-[(phenylmethyl)thiol-2H--pyran-2-one ; The title compound was prepared according to method A , using 4'-[2-(4-morpholin-yl)ethoxy]acetophenone (1.31 g, 5.29 mmol) lithium bis(trimethylsilyl)amide (0.972 g, 5.81 mmol) chlorotrimethylsilane (0.738 mL, 5.81 mmol), THF ( 58 mL) and diethyl [(phenylmethyl)thio]propanedioate (1.35 g, 4.80 mmol). Melting point 207 °C with decomposition; 1H NMR (250 MHz, DMSO-d6) δ 2.54 (s, 2H), 6.89 (m, 4H), 2.83 (t, 3H), 3.55 (m, 4H), 3.96 (s, 2H), 4.22 (t, 2H), 6.58 (s, 1H), 7.08 (d, 2H), 7.23 (m, 5H), 7.73 (d, 2H). Example 15 4-Hydroxy-6-(2-naphthalenyl)-3-[(phenylmethyl)thio]-2H-pyran-2-one The title compound was prepared according to method A, using 2-acetylnaphthalene (1.97 g, 11.6 mmol) lithium bis(trimethylsilyl)amide (2.13 g, 12.76 mmol) chlorotrimethylsilane (1.61 mL, 12.76 mmol), THF (127 mL) and diethyl [(phenylmethyl)thio]propanedioate (2.90 g, 10.5 mmol). Melting point 203 °C with decomposition; 1H NMR (250 MHz, DMSO-d6) δ 4.04 (s, 2H), 6.89 (s, 1H), 7.23 (m, 5H), 7.61 (m, 2H), 7.84 (d, 2H), 8.05 (m, 3H), 8.43 (s, IH), 11.95 (wide s, IH). ;Example 16 ;4-Hydroxy-6-phenyl-3-[(phenylthio)methyl]-2H-pyran-2-one ;The title compound was prepared according to method C, using 4-hydroxy-6-phenyl-2H-pyran- 2-one (1.00 g, 5.31 mmol), ethanol (10 mL), paraformaldehyde (0.175 g, 5.80 mmol), thiophenol (1.40 mL, 13.8 mmol), piperidine (0.5 mL), and acetic acid (0.5 mL). Melting point 203 °C with decomposition; 1H NMR (250 MHz, DMSO-d6) δ 3.98 (s, 2H), 6.73 (s, IH), 7.17 (m, IH), 7.31 (m, 2H), 7.37 (m, 2H), 7.54 (m, 3H), 7.77 (m, 2H), 12.05 (broad s, IH). Example 17 4-Hydroxy-6-(4-hydroxyphenyl)-3-[(phenylthio)methyl]-2H-pyran-2-one The title compound was prepared according to method A, using 4'-hydroxyacetophenone (0.722 g, 5.31 mmol) lithium bis(trimethylsilyl)amide (1.95 g, 11.6 mmol) chlorotrimethylsilane (1.48 mL, 11.6 mmol), THF (116 mL) and diethyl [(phenylmethyl)thio]propanedioate (1.0 g, 3.54 mmol). Melting point 204 °C with decomposition; 1H NMR (250 MHz, DMSO-d6) δ 3.96 (s, 2H), 6.55 (s, 1H), 6.88 (d, 2H), 7.39 (m, 5H), 7.63 (d, 2H), 10.28 (s, IH), 11.75 (wide with, IH). Example 18 4-Hydroxy-6-(4-hydroxyphenyl)-3-[(phenylthio)inethyl]-2H-pyran-2-one The title compound was prepared according to method A, using 4'-methoxyacetophenone (0.797 g, 5.31 mmol) lithium bis(trimethylsilyl)amide (0.977 g, 5.84 mmol) chlorotrimethylsilane (0.741 mL, 5.84 mmol), THF (58 mL) and diethyl [(phenylmethyl)thio]propanedioate (1.0 g, 3.54 mmol). Melting point 187 °C with decomposition; 1H NMR (250 MHz, DMSO-d6) δ 3.83 (s, 3H), 3.98 (s, 2H), 6.62 (s, 1H), 7.06 (m, 2H), 7.22 (m, 5H), 7.73 (m, 2H), 11.76 (wide s, IH). Example 19 4-Hydroxy-6-(4-methylphenyl)-3-[(phenylthio)methyl]-2H-pyran-2-one The title compound was prepared according to method A, using 4'-methylacetophenone (0.712 g, 5.31 mmol) lithium bis(trimethylsilyl)amide (0.977 g, 5.84 mmol) chlorotrimethylsilane (0.741 mL, 5.84 mmol), THF (58 mL) and diethyl [(phenylmethyl)thiopropanedioate (1.0 g, 3.54 mmol). Melting point 205 °C with decomposition; 1H NMR (400 MHz, DMSO-d6) δ 2.37 (s, 3H), 3.99 (s, 2H), 6.69 (s, IH), 7.26 (m, 7H), 7.68 (m, 2H), 11.83 (broad s , IH). ;Example 20 ;3-[Bis(phenylmethyl)amino]-4-hydroxy-6-phenyl-2H-pyran-2-one ;The title compound was prepared according to method D, using 3-amino-4-hydroxy-6-phenyl -2H--pyran-2-one hydrochloride (0.150 g, 0.626 mmol), 1% acetic acid in dimethylibrmamide (7 mL), benzaldehyde (0.133 mL, 1.13 mmol) and sodium cyanoborohydride (0.083 g, 1.31 mmol). Melting point 130-135 °C; 1H NMR (400 MHz, DMSO-d6) δ 4.26 (s, 4H), 6.44 (s, 1H), 7.24 (m, 6H), 7.44 (m, 7H), 7.69 (m, 2H). ;Example 21 ;4-Hydroxy-6-phenyl-3-[2-(phenylethyl)thio]-2H-pyran-2-one ;The title compound was prepared according to method B, using 4-hydroxy-6-phenyl-2H- pyran-2-one (0.500 g, 2.65 mmol), ethanol (7 mL), 1M sodium hydroxide (2.65 mL) and 2-phenylethyl-p-toluenethiosulfonate (0.770 g, 2.65 mmol). Melting point 121-124 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.78 (t, 2H), 2.99 (t, 2H), 6.80 (s, 1H), 7.24 (m, 5H), 7.54 (m, 3H), 7.80 (m, 2H). ;Example 22 ;4-Hydroxy-6-phenyl-3-[(3-phenylpropyl)thio]-2H-pyran-2-one ;The title compound was prepared according to method A, using 1-phenyl-1-(trimethylsilyloxo)ethylene (0.922 g, 4.83 mmol) and diethyl [(3-phenylpropyl)thio]propanedioate (1.0 g, 3.22 mmol). Melting point 114-116 °C; 1H NMR (400 MHz, DMSO-d6) δ 1.74 (m, 2H), 2.71 (m, 4H), 6.82 (m, 1H), 7.16 (m, 3H), 7.25 (m, 2H), 7.54 (m, 3H), 7.81 (m, 2H), 11.95 (broad s, IH). ;Example 23 ;4-Hydroxy-3-[2-(phenyloxyethyl)thio]-6-phenyl-2H-pyran-2-one ;The title compound was prepared according to method B, using 4-hydroxy-6-phenyl-2H- pyran-2-one (0.500 g, 2.65 mmol), ethanol (7 mL), 1M sodium hydroxide (2.65 mL, 2.65 mmol) and 2-phenoxyethyl-p-toluenethiosulfonate (0.816 g, 2.65 mmol). Melting point 146-149°C; 1H NMR (400 MHz, DMSO-d6) δ 3.12 (t, 2H), 4.11 (t, 2H), 6.81 (s, 1H), 6.88 (m, 3H), 7.24 (m, 2H), 7.54 (m, 3H), 7.81 (m, 2H), 12.04 (broad s, IH). Example 24 4-Hydroxy-6-(2-methylphenyl)-3-[(phenylmethyl)thio]-2H-pyran-2-one The title compound was prepared according to method A, using 2'-methylacetophenone (0.712 g, 5.31 mmol) lithium bis(trimethylsilyl)amide (0.977 g, 5.84 mmol) chlorotrimethylsilane (0.741 mL, 5.84 mmol), THF (58 mL) and diethyl [(phenylmethyl)thiopropanedioate (1.0 g, 3.54 mmol). 1H NMR (400 MHz, DMSO-d6) δ 2.34 (s, 3H), 4.01 (s, 2H), 6.32 (s, 1H), 7.32 (m, 9H). ;Example 25 ;4-Hydroxy-6-(2-phenylethyl)-3-[(phenylmethyl)thio]-2H-pyran-2-one ;The title compound was prepared according to method A, using 4-phenethylacetophenone (0.786 g, 5.31 mmol) lithium bis(trimethylsilyl)amide (0.977 g, 5.84 mmol) chlorotrimethylsilane (0.741 mL, 5.84 mmol), THF (58 mL) and diethyl [(phenylmethyl)thiopropanedioate (1.0 g, 3.54 mmol). Melting point 164-166 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.75 (t, 3H), 2.85 (t, 2H), 3.92 (s, 2H), 5.92 (s, IH), 7.23 (m, 9H), 11.69 (broad s , IH). ;Example 26 ;4-Hydroxy-6-(3-hydroxyphenyl)-3-[(phenylmethyl)thio]-2H-pyran-2-one ;The title compound was prepared according to method A, using 3'-hydroxyketophenone (0.722 g, 5.31 mmol) lithium bis(trimethylsilyl)amide (1.95 g, 11.6 mmol) chlorotrimethylsilane (1.48 mL, 11.6 mmol), THF (116 mL) and diethyl [(phenylmethyl)thio]propanedioate (1.0 g, 3.54 mmol). Melting point 185 °C with decomposition. 1H NMR (400 MHz, DMSO-d6) δ 4.00 (s, 2H), 6.66 (s, 1H), 6.92 (m, 1H), 7.21 (m, 7H), 7.32 (m, 1H). Example 27 4-Hydroxy-6-(4-hydroxyphenyl)-3-[(2-phenylethyl)thio]-2H-pyran-2-one The title compound was prepared according to method A, using 4'-hydroxyacetophenone (0.688 g, 5.06 mmol) lithium bis(trimethylsilyl)amide (1.84 g, 11.1 mmol) chlorotrimethylsilane (1.41 mL, 11.1 mmol), THF (111 mL) and diethyl [(2-phenylethyl)thio]propanedioate (1.0 g, 3.37 mmol) ). 1H NMR (400 MHz, DMSO-d6) δ 2.78 (t, 2H), 2.95 (t, 2H), 6.62 (s, IH), 6.89 (dd, IH), 7.21 (m, 5H), 7.65 (d, 2H), 10.22 (s, IH), 11.05 (wide s, IH). ;Example 28 ;4-Hydroxy-6-phenyl-3-[3-(phenyl-2-propenyl)thio]-2H-pyran-2-one ;The title compound was prepared according to method B, using 4-hydroxy-6- phenyl-2H-pyran-2-one (0.500 g, 2.65 mmol), ethanol (7 mL), 1 M sodium hydroxide (2.65 mL) and 3-phenyl-2-propenyl-p-toluenethiosulfonate (0.808 g, 2.65 mmol) ). Melting point 133-136°C; 1H NMR (400 MHz, DMSO-d6) δ 3.57 (d, 2H), 6.24 (dt, 2H), 6.76 (s, 1H), 7.24 (m, 5H), 7.51 (m, 3H), 7.78 (m, 2H). Example 29 4-Hydroxy-3-[(2-phenylethyl)thio]-6-[4-(phenylmethoxy)phenyl]-2H-pyran-2-one The title compound was prepared according to method A, using 4'- benzoyloxyacetophenone (1.14 g, 5.06 mmol) lithium bis(trimethylsilyl)amide (0.930 g, 5.56 mmol) chlorotrimethylsilane (0.705 mL, 5.56 mmol), THF (57 mL) and diethyl [(2-phenylethyl)thio]propanedioate (1.0 g) , 3.37 mmol). Melting point 139-142 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.77 (t, 2H), 2.98 (t, 2H), 5.19 (s, 2H), 6.68 (s, 1H), 7.26 (m, 7H), 7.43 (m, 5H), 7.76 (d, 2H). Example 30 4-Hydroxy-6-[4-(2-phenylethoxy)phenyl]-3-[(2-phenylethyl)thio]-2H-pyran-2-one The title compound was prepared according to method A, using 4 of '-(2-phenylethoxy)acetophenone (1.21 g, 5.06 mmol) lithium bis(trimethylsilyl)amide (0.930 g, 5.56 mmol) chlorotrimethylsilane (0.705 mL, 5.56 mmol), THF (57 mL) and diethyl [(2-phenylethoxy) )thio]propanedioate (1.0 g, 3.37 mmol). Melting point 103-106 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.76 (t, 2H), 2.97 (t, 2H), 3.06 (t, 2H), 4.27 (t, 2H), 6.67 (s, IH), 7.21 (m, 12H), 7.73 (d, 2H). Example 31 4-Hydroxy-3-[(2-phenylethyl)thio]-6-[4-(4-phenylpropoxy)phenyl]-2H-pyran-2-one The title compound was prepared according to method A, using 4 of '-(3-phenylpropoxy)acetophenone (1.28 g, 5.06 mmol) lithium bis(trimethylsilyl)amide (0.930 g, 5.56 mmol) chlorotrimethylsilane (0.705 mL, 5.56 mmol), THF (57 mL) and diethyl [(2-phenylethyl )thio]propanedioate (1.0 g, 3.37 mmol). Melting point 139-142 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.04 (m, 2H), 2.84 (m, 4H), 2.98 (t, 2H), 4.40 (t, 2H), 6.68 (s, IH), 7.18 (m, 12H), 7.75 (d, 2H), 11.86 (broad s, IH). Example 32 4-Hydroxy-6-(2-hydroxyphenyl)-3-[(phenylmethyl)thio]-2H-pyran-2-one The title compound was prepared according to method A, using 2'-hydroxyacetophenone (0.722 g, 5.31 mmol) lithium bis(trimethylsilyl)amide (1.95 g, 11.6 mmol) chlorotrimethylsilane (1.48 mL, 11.6 mmol), THF (116 mL) and diethyl [(phenylmethyl)thiopropanedioate (1.0 g, 3.54 mmol). Melting point 189 °C with decomposition; 1H NMR (400 MHz, DMSO-d6) δ 4.01 (s, 2H), 6.97 (s, IH), 7.25 (m, 7H), 7.71 (d, IH), 10.75 (s, IH), 11.85 (broad s , IH). ;Example 33 ;4-Hydroxy-6-[3-(2-phenylethoxy)phenyl]-3-[(2-phenylethyl)thio]-2H-pyran-2-one ;The title compound was prepared according to method A, using 3 of '-(2-phenylethoalkyl)acetophenone (0.336 g, 1.40 mmol) lithium bis(trimethylsilyl)amide (10.257 g, 1.54 mmol) chlorotrimethylsilane (0.195 mL, 1.54 mmol), THF (15 mL) and diethyl [(2-phenylethyl) )thio]propanedioate (0.417 g, 1.40 mmol). Melting point 104-106 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.75 (t, 2H), 2.97 (t, 2H), 3.04 (t, 2H), 4.25 (t, 2H), 6.79 (s, IH), 7.25 (m, 14H), 11.95 (wide with, IH). ;Example 34 ;4-Hydroxy-6-phenyl-3-[phenyl(phenylthio)methyl]-2H-pyran-2-one (+/-) ;The title compound was prepared according to method E, using 4-hydroxy-6- phenyl-2H-pyran-2-one (1.00 g, 5.31 mmol), ethanol (10 mL). benzaldehyde (0.593 mL, 5.84 mmol), thiophenol (1.40 mL, 13.8 mmol), piperidine (0.5 mL) and acetic acid (0.5 mL). Decomposition >220 °C; 1H NMR (400 MHz, DMSO-d6) δ 5.80 (s, 1H), 6.70 (s, 1H), 7.23 (m, 8H), 7.54 (m, 4H), 7.74 (m, 2H). ;Example 35 ;4-Hydroxy-3-[[2-(2-methoxyphenyl)ethyl]thio]-6-phenyl-2H-pyran-2-one ;The title compound was prepared according to method B, using 4-hydroxy-6 -phenyl-2H-pyran-2-one (0.500 g, 2.65 mmol), ethanol (7 mL), 1 M sodium hydroxide (2.65 mL) and 2-(2-methoxy-phenyl)ethyl -p-toluenethiosulfonate (0.856 g, 2.65 mmol). Melting point 114-115 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.74 (t, 2H), 2.94 (t, 2H), 3.73 (s, 1H), 6.85 (m, 3H), 7.15 (m, 2H), 7.54 (m, 3H), 7.82 (m, 2H). ;Example 36 ;4-Hydroxy-6-phenyl-3-[(4-phenylbutyl)thio]-2H-pyran-2-one ;The title compound was prepared according to method B, using 4-hydroxy-6-phenyl-2H- pyran-2-one (0.500 g, 2.65 mmol), ethanol (7 mL), 1 M sodium hydroxide (2.65 mL) and 4-phenylbutyl-p-toluenethiosulfonate (0.851 g, 2.65 mmol). Melting point 103-105 °C; 1H NMR (400 MHz, DMSO-d6) δ 1.47 (m, 2H), 1.66 (m, 2H), 2.54 (t, 2H), 2.77 (t, 2H), 6.80 (s, IH), 7.17 (m, 5H), 7.53 (m, 3H), 7.81 (m, 2H). ;Example 37 ;4-Hydroxy-3-[[2-(3-methoxyphenyl)ethyl]thio]-6-phenyl-2H-pyran-2-one ;The title compound was prepared according to method B, using 4-hydroxy-6 -phenyl-2H-pyran-2-one (0.500 g, 2.65 mmol), ethanol (7 mL), 1 M sodium hydroxide (2.65 mL) and 2-(3-methoxy-phenyl)ethyl-?-toluenethiosulfonate ( 0.856 g, 2.65 mmol). Melting point 1112-113 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.75 (t, 2H), 3.01 (t, 2H), 3.34 (s, 3H), 6.75 (s, IH), 7.16 (t, IH), 7.54 (m, 3H), 7.80 (m, 2H). ;Example 38 ;4-Hydroxy-3-[[2-(4-methoxyphenyl)ethyl]thio]-6-phenyl-2H-pyran-2-one ;The title compound was prepared according to method B, using 4-hydroxy-6 -phenyl-2H-pyran-2-one (0.500 g, 2.65 mmol), ethanol (7 mL), 1 M sodium hydroxide (2.65 mL) and 2-(4-methoxy-phenyl)ethyl-p-toluenethiosulfonate (0.856 g, 2.65 mmol). Melting point 144-145 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.71 (t, 2H), 2.96 (t, 2H), 3.66 (s, 3H), 6.77 (s, 1H), 6.80 (d, 2H), 7.12 (d, 2H), 7.54 (m, 3H), 7.80 (m, 2H). ;Example 39 ;3-[[2-(3-Chlorophenyl)ethyl]thio]-4-hydroxy-6-phenyl-2H-pyran-2-one ;The title compound was prepared according to method B, using 4-hydroxy-6 -phenyl-2H-pyran-2-one (0.500 g, 2.65 mmol), ethanol (7 mL), 1M sodium hydroxide (2.65 mL) and 2-(2-chloro-phenyl)ethyl-p-toluenethiosulfonate (0.868 g , 2.65 mmol). Melting point 133-134°C; 1H NMR (400 MHz, DMSO-d6) δ 2.79 (t, 2H), 3.02 (t, 2H), 6.77 (s, 1H), 7.25 (m, 4H), 7.55 (m, 3H), 7.81 (m, 2H). Example 40 4-Hydroxy-6-phenyl-3-(2-phenylethyl)-2H-pyran-2-one The title compound was prepared according to method F, using Raney nickel (Grace 3100), ethanol (20 mL), 4-hydroxy-6-phenyl-3-[2-phenyl-1-(phenylthio)ethyl]-2H-pyran-2-one (0.425 g, 1.06 mmol). Decomposition >255 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.65 (dd, 2H), 2.71 (dd, 2H), 6.68 (s, 1H), 7.23 (m, 3H), 7.52 (m, 3H), 7.76 (m, 2H), 11.85 (wide s, IH). ;Example 41 ;4-Hydroxy-6-phenyl-3-(3-phenylpropyl)-2H-pyran-2-one ;The title compound was prepared according to method F, using Raney nickel (Grace 3100), ethanol (15 mL), 4-hydroxy-6-phenyl-3-[3-phenyl-1-(phenylthio)propyl]-2H-pyran-2-one (0.150 g, 0.362 mmol). Melting point 195-196 °C; 1H NMR (400 MHz, DMSO-d6) δ 1.73 (m, 2H), 2.40 (t, 2H), 2.60 (t, 2H), 6.68 (s, 1H), 7.23 (m, 5H), 7.52 (m, 3H), 7.74 (m, 2H). Example 42 6-(2,6-Dimethylphenyl)-4-hydroxy-3-[(phenylethyl)thio]-2H-pyran-2-one The title compound was prepared according to method A, using 2',6'- dimethylacetophenone (0.785 g, 5.31 mmol) lithium bis(trimethylsilyl)amide (0.977 g, 5.84 mmol) chlorotrimethylsilane (0.741 mL, 5.84 mmol), THF (58 mL) and diethyl [(phenylmethyl)thio]propanedioate (1.00 g, 3.54 mmol). Melting point 140-143 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.15 (m, 6H), 3.99 (s, 2H), 6.12 (s, 1H), 7.22 (m, 8H). Example 43 4-Hydroxy-6-[2-hydroxy-3-methyl-4-(phenylmethoxy)phenyl]-3-[(2-phenylethyl)-thio]-2H-pyran-2-one The title compound is prepared according to method A, using 4'-benzyloxy-2'-hydroxy-3'-methyl-acetophenone (1.29 g, 5.06 mmol) lithium bis(trimethylsilyl)amide (2.11 g, 12.6 mmol) chlorotrimethylsilane (1.60 mL, 12.6 mmol) ), THF (58 mL) and diethyl [(2-phenylethyl)thio]-propanedioate (1.00 g, 3.37 mmol). Melting point 147-148 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.14 (s, 3H), 2.77 (t, 2H), 2.98 (t, 2H), 5.17 (s, 2H), 5.29 (s, 1H), 6.79 (d, IH), 7.30 (m, 13H), 9.36 (s, IH), 11.85 (wide s, IH). ;Example 44 ;4-Hydroxy-3-[(2-phenylethyl)thio]-6-[3-(phenylmethoxy)phenyl]-2H-pyran-2-one ;The title compound was prepared according to method A, using 3'- benzyloxyacetophenone (1.14 g, 5.06 mmol) lithium bis(trimethylsilyl)amide (0.930 g, 5.56 mmol) chlorotrimethylsilane (0.705 mL, 5.56 mmol), THF (57 mL) and diethyl [(2-phenylethyl)thio]propanedioate (1.00 g) , 3.37 mmol). Melting point 126-127 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.87 (t, 2H), 3.01 (t, 2H), 5.20 (s, 2H), 6.81 (s, 1H), 7.22 (m, 6H), 7.41 (m, 7H). ;Example 45 ;4-Hydroxy-6-[4-(2-naphthalenylmethoxy)phenyl]-3-[(2-phenylethyl)thio]-2H-pyran--2-one ;The title compound was prepared according to method A, using 4'-(2-Naphthalenylmethoxy)acetophenone (1.39 g, 5.06 mmol) lithium bis(trimethylsilyl)amide (0.930 g, 5.56 mmol) chlorotrimethylsilane (0.705 mL, 5.56 mmol), THF (57 mL) and diethyl [(2- phenylethyl)thio]propanedioate (1.00 g, 3.37 mmol). Melting point 152-154 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.77 (t, 2H), 2.98 (t, 2H), 5.38 (s, 2H), 6.68 (s, IH), 7.21 (m, 7H), 7.54 (m, 2H), 7.60 (d, 1H), 7.96 (m, 4H). Example 46 6-(3-chloro-4-methoxyphenyl)-4-hydroxy-3-[(phenylmethyl)thio]-2H-pyran-2-one The title compound was prepared according to method A, using 3'-chloro -4'-methoxyacetophenone (0.979 g, 5.84 mmol) lithium bis(trimethylsilyl)amide (0.977 g, 5.84 mmol) chlorotrimethylsilane (0.741 mL, 5.84 mmol), THF (58 mL) and diethyl [(phenylmethyl)thio]propanedioate ( 1.00 g, 3.54 mmol). Melting point 171 °C with decomposition; 1H NMR (400 MHz, DMSO-d6) δ 3.93 (s, 3H), 3.99 (s, 2H), 6.68 (s, IH), 7.32 (m, 6H), 7.77 (s, IH), 7.83 (d, THEM). ;Example 47 ;4-Hydroxy-6-phenyl-3-[(phenylmethyl)sulfonyl]-2H-pyran-2-one ;This compound was prepared by oxidation of 4-hydroxy-6-phenyl-3-[(phenylmethyl)thio] -2H-pyran-2-one (1 mmol, 310 mg) with oxone (3 mmol, 1.99 g) at room temperature in 10 mL of methanol and 10 mL of water. After the reaction mixture was stirred for 4 hours at room temperature, it was diluted with water and extracted with 50 mL of dichloromethane. The organic layer was dried over magnesium sulfate. Solvents were evaporated. The rest was a pure compound according to tcl. Yield of the isolated compound: 90%, melting point 152-153 °C. 1H NMR (400 MHz, CDCl3) δ 11.34 (s, 1H), 7.8 (m, 2H), 7.5 (m, 3H), 7.37 (m, 3H), 7.27 (m, 2H), 6.37 (s, 1H) , 6.23 (s, 1H), 4.75 (s, 1H), 4.34 (q, 2H); IR (KBr) 3421, 3059, 1726, 1698, 1628, 1559, 1497, 1230, 957, 770, 689 cm-1; MS (CI) m/e 343 (6.8), 327 (15.54, 287 (15.99). 219 (40.99, 91 (100). ; Example 48 ; 4-Hydroxy-6-(3-methylphenyl)-3-[( phenylmethyl)thio]-2H-pyran-2-one; This compound was prepared by condensation of diethyl [(phenylmethyl)thio]propanedioate (1 g, 3.54 mmol) and the corresponding trimethylsilylenoi-ether of 3'-methoxyacetophenone (7.09 mmol, 1.46 g) , as described in general method A. Yield of isolated product: 65%, mp 137-138 °C. 1H NMR (400 MHz, DMSO-d6) δ 11.9 (broad s, 1H), 7.6 (m, 2H), 7.39 (t, IH), 7.35 (d, IH), 7.25 (d, 4H), 7.2 (m, IH), 6.7 (s, IH), 4.0 (s, 2H), 2.38 (s, 3H); IR (KBr) 3030, 2585, 1617, 1536, 1402, 1100, 787, 698 cm-1; MS (CI) m/e 325 (65), 291 (2), 233 (4), 119 (9), 91 (100). ;Example 49 ;2-Oxo-6-phenyl-3-[(phenylmethyl)thio]-2H-pyran-4-ylpropionate ;This compound was prepared by the reaction of the sodium salt of 4-hydroxy-6-phenyl-3- [(phenylmethyl)]thio]-2H-pyran-2-one (310 g, 1 mmol) and propionyl chloride (2.4 mmol, 222 mg), as described in general procedure G. Use of the isolated product: 72%. 1H NMR (400 MHz, DMSO-d6) δ 7.77 (m, 2H), 7.51 (m, 3H), 7.22 (m, 4H), 7.17 (m, IH), 6.7 (s, IH), 3.98 (s, 2H), 2.19 (q,2H), 0.96 (t,3H); IR (KBr) 3438, 3027, 2923, 1772, 1731, 1617, 1528, 1494, 1453, 1323, 1153, 1087, 1045, 979, 873 , 767, 702 cm-1; MS (CI) m/e 366 (4), 311 (79), 189(26), 105(20.91 (100). ; Example 50 ; 4-Hydroxy-6-[3-methyl-4-(phenylmethyloxy) )phenyl]-3-[(2-phenylmethyl)thiol-2H-pyran-2-one; Condensation of diethyl [(2-phenylethyl)thio]propanedioate (1.06 g, 3.6 mmol) and trimethylsilylenol--ether 3'-methoxy- 4'- benzyloxyacetophenone (2.24 g, 7.2 mmol)), was performed as described in general procedure A. Yield of the isolated product: 78%, melting point 147-148 °C. 1H NMR (400 MHz, DMSO-d6) δ 7.63 (m, 2H), 7.11-7.53 (m, UH), 6.68 (s, IH), 5.22 (s, IH), 2.98 (t, 2H), 2.77 ( t, 2H), 2.27 (s, 3H); IR (KBr) 3432, 3030, 2922, 1717, 1626, 1503, 1408, 1262, 1140, 1024, 696 cm-1; MS (CI) m/e 445 (2.12), 353.34, 309 (3.81), 189 (8.33), 156 (14.78), 137 (16.19), 105 (94.34) 91 (100); Elemental analysis, calculated: C, 72.95; H, 5.44; found: C, 72.25; H, 5.43. ;Example 51 ;4-Hydroxy-6-(4-hydroxy-2-methylphenyl)-3-[(2-phenylethyl)thio]-2H-pyran-2-one ;This compound was prepared by condensation of diethyl [(2-phenylethyl )thio]propanedioate (1 g, 3.38 mmol) and the corresponding trimethylsilylenol ether 4'-hydroxy-2l-methylacetophenone (2.94 g, 10 mmol), as described in general procedure A. Yield of the isolated product: 52%, melting point 85- 87 °C. 1H NMR (400 MHz, DMSO-d6) δ 11.89 (broad s, IH), 9.97 (s, IH), 7.35 (d, IH), 7.23 (m, 5H), 6.72 (s, 2H), 6.33 (s , 1H), 3.0 (t, 2H), 2.78 (t, 2H), 2.34 (s, 3H); IR (KBr) 3300, 2926, 1672, 1604, 1541, 1244, 1194, 1120, 698 cm-1; MS (CI) m/e 355 (36), 250 (27), 105 (93), 91 (30), 85 (100). Elemental analysis, calculated: C, 67.78; H, 5.12; found: C, 67.53; H, 5.40. ;Example 52 ;4-Hydroxy-6-(4-hydroxy-3-methylphenyl)-3-[(phenylmethyl)thio]-2H-pyran-2-one ;This compound was prepared by condensation of diethyl [(phenylmethyl)thio]propanedioate (1 g) and the corresponding trimethylsilylenol ether 4'-hydroxy-3'-methylacetophenone as described in general procedure A. Recovery of the isolated product: 68%, melting point 159-166 °C. 1H NMR (400 MHz, DMSO-d6) δ 7.67 (dd, IH), 7.61 (s, IH), 7.2 (m, 5H), 6.8 (d, IH), 6.38 (s, IH), 3.96 (s, 2H) 3.89 (s, 3H), 2.25 (s, 3H); IR (KBr) 3432, 2945, 1613, 1507, 1402, 1262, 1142, 1030, 812, 704 cm-1; MS (CI) m/e 355 (78.3), 263 (19.6), 235 (11.8). 149 (12.7), 91 (100). Elemental analysis, calculated: C, 67.78; H, 5.12; found: C, 67.35; H, 5.17. Example 53: 2-Oxo-6-phenyl-3-[(phenylmethyl)thio]-2H-pyran-4-ylacetate; This compound was prepared by the reaction of the sodium salt of 4-hydroxy-6-phenyl-3-[(phenylmethyl) ]thio]-2H-pyran-2-one (310 g, 1.00 mmol) and acetyl chloride (188 mg, 2.4 mmol), as described in general procedure G. Yield of the isolated product: 72%. 1H NMR (400 MHz, DMSO-d6) δ 7.81 (m, 2H), 7.53 (m, 3H), 7.22 (m, 4H), 7.16 (m, 1H), 3.99 (s, 2H), 1.92 (s, 2H). Example 54 2-Oxo-6-phenyl-2H-pyran-4-yl-1-naphthalenecarboxylate This compound was prepared according to method G, using 4-hydroxy-6-phenyl-2H-pyran-2-one (0.250 g, 1.32 mmol), THF (15 mL), 60% sodium hydride (0.585 g, 1.46 mmol) and 1-naphthoyl chloride (0.278 g, 1.46 mmol). Melting point 123.5-125 °C; 1H NMR (400 MHz, DMSO-d6) δ 6.54 (m, IH), 7.49 (s, IH), 7.65 (m, 6H), 7.95 (m, 2H), 8.13 (d, IH), 8.34 (d, IH), 8.50 (d, IH). ;Example 55 ;3,3'-Thiobis[4-hydroxy-6-phenyl-2H-pyran-2-one] ;This compound was prepared by the following method: 4-Hydroxy-6-phenyl-2H-pyran-2-one (0.250 mg, 1.33 mmol) was gradually added thionyl chloride (0.585 mL). The reaction was allowed to stir overnight at room temperature. Unreacted thionyl chloride was removed in vacuo, and the residue was recrystallized from boiling methanol, melting point >240 °C; 1H NMR (400 MHz, d-TFA) δ 7.03 (s, 2H), 7.56 (m, 6H), 7.89 (m, 4H). Example 56 3,3'-Dithiobis[4-hydroxy-6-phenyl-2H-pyran-2-one] Sulfur monochloride (0.105 mL, 1.32 mmol) was dissolved in benzene (1 mL), and the solution a suspension of 4-hydroxy-6-phenyl-2H-pyran-2-one (0.500 g, 2.65 mmol) in benzene (7 mL) was added dropwise, whereby the suspension was refluxed. Refluxing continued for 1.5 hours. The reaction was "quenched" with a few drops of water, and the light product was collected by filtration. The solid was recrystallized from boiling acetic acid. Seedlings >280 °C; 1H NMR (400 MHz, DMSO-d6) δ 6.77 (s, 2H), 7.52 (m, 6H), 7.81 (m, 4H). ;Example 57 ;3-Benzoyl-4-hydroxy-6-phenyl-2H-pyran-2-one ;A trace amount of sodium bicarbonate was added to a solution of ethyl benzoylacetate (150 g, 0.88 mmol) in 1,2-dichlorobenzene (150 mL) . The reaction mixture was heated to reflux. Ethanol was collected as a distillate (about 20 mL). The reaction mixture was cooled to 0 °C. Ether (100 mL) was added to induce crystallization. The reaction mixture was kept in a refrigerator overnight. The resulting crystals were collected and washed with ether. Melting point 171-173 °C; (250 MHz, DMSO-d6) δ 6.91 (s, 1H), 7.59 (m, 6H), 7.87 (m, 4H). ;Example 58 ;N-(4-Hydroxy-2-oxo-6-phenyl-2H-pyran-3-yl)benzeneacetamide ;The title compound was prepared according to method E, using 3-amino-4-hydroxy-6-phenyl- 2H-pyran-2-one hydrochloride (1.50 g, 0.626 mmol), methylene chloride (6 mL), triethylamine (0.348 mL, 2.50 mmol) and catalyst 4-dimethylaminopyridine phenacetyl chloride (0.106 g, 0.626 mmol). Melting point 213 °C with decomposition; 1H NMR (250 MHz, DMSO-d6) δ 3.69 (s, 2H), 6.85 (s, IH), 7.29 (m, 4H), 7.53 (m, 3H), 7.83 (m, 2H), 9.40 (broad s , IH). Example 59 2-Oxo-6-phenyl-2H-pyran-4-yl-2-naphthalenecarboxylate This compound was prepared according to method G, using 4-hydroxy-6-phenyl-2H-pyran-2-one (0.200 g, 0.835 mmol), methylene chloride (8 mL), triethylamine (0.348 mL, 2.50 mmol) and the catalyst 4-dimethylaminopyridine 2-naphthoyl chloride (0.175 g, 0.918 mmol). Melting point 143.5-144 °C; 1H NMR (250 MHz, DMSO-d6) δ 6.51 (s, IH), 7.51 (m, 3H), 7.72 (m, 3H), 8.80 (m, 7H), 8.89 (broad s, IH). ;Example 60 ;3-[Bis(2-naphthalenylmethyl)amino]-4-hydroxy-6-phenyl-2H-pyran-2-one ;This compound was prepared according to method D, using 3-amino-4-hydroxy-2H -pyran-2-one hydrochloride (0.250 g, 1.04 mmol), 1% acetic acid in dimethylfoemamide (10 mL), 2-naphthalaldehyde (0.407 g, 2.60 mmol) and sodium cyanoborohydrin (0.164 g, 2.60 mmol). Melting point 209 °C with decomposition; 1H NMR (250 MHz, DMSO-d6) δ 4.46 (s, 4H), 6.38 (s, 1H), 7.44 (m, 8H), 7.77 (m, 13H). ;Example 61 ;N-(4-Hydroxy-2-oxo-6-phenyl-2H-pyran-3-yl)2-naphthaleneacetamide ;The title compound was prepared according to method E, using 3-amino-4-hydroxy-6- phenyl-2H-pyran-2-one hydrochloride (0.200 g, 0.835 mmol), THF (9 mL). 60% sodium hydride (0.037 mL, 0.918 mmol) oxalyl chloride (0.080 mL, 0.918 mmol) and 2-naphthalylacetic acid (0.170 g, 0.928 mmol). Melting point 227 °C with decomposition; 1H NMR (250 MHz, DMSO-d6) δ 4.17 (s, 2H), 6.84 (s, IH), 7.50 (m, 6H), 7.83 (m, 4H), 7.93 (d, IH), 8.17 (d, IH), 9.58 (s, IH). ;Example 62 ;N-(4-Hydroxy-2-oxo-6-phenyl-2H-pyran-3-H)-2-naphthalenecarboxamide ;The title compound was prepared according to method E, using 3-amino-4-hydroxy-6 -phenyl-2H-pyran-2-one hydrochloride (0.150 g, 0.626 mmol), THF (6 mL). 60% sodium hydride (0.028 mL, 0.688 mmol) and 2-naphthoyl chloride (0.131 mL, 0.688 mmol). Melting point 219 °C with decomposition; 1H NMR (250 MHz, DMSO-d6) δ 6.92 (s, 1H), 7.61 (m, 5H), 7.97 (m, 6H), 8.62 (s, 1H), 9.61 (s, 1H). Melting point 87-90 °C; 1H NMR (400 MHz, DMSO-d6) δ 1.46 (m, 5H), 1.61 (m, 4H), 2.15 (m, IH), 2.31 (d, IH), 4.26 (d, IH), 6.65 (s, 1H), 7.16 (t, 1H), 7.27 (t, 2H), 7.37 (d, 2H), 7.52 (m, 3H), 7.74 (m, 2H), 11.80 (broad s, 1H). ;Example 63 ;N-(4-Hydroxy-2-oxo-6-phenyl-2H-pyran-3-yl)benzenepropionamide ;The title compound was prepared according to method E, using 3-amino-4-hydroxy-6-phenyl- 2H-pyran-2-one hydrochloride (0.150 g, 0.626 mmol), THF (6 mL). 60% sodium hydride (0.028 mL, 0.688 mmol) and hydrocinnamoyl chloride (0.131 mL, 0.688 mmol). Melting point 191-193 °C; 1H NMR (250 MHz, DMSO-d6) δ 2.65 (t, 2H), 2.89 (t, 2H), 6.86 (s, 1H), 7.26 (m, 5H), 7.53 (m, 3H), 7.84 (m, 2H), 9.28 (s, 1H). ;Example 64 ;6-(1,3-BenzodioxoI-5-yl)-4-hydroxy-3-[(phenylmethyl)thio]-2H-pyran-2-one ;The title compound was prepared according to method A, using 3' ,4'-(methylenedioxy)acetophenone (0.871 g, 5.31 mmol) lithium bis(trimethylsilyl)amide (0.977 g, 5.84 mmol) chlorotrimethylsilane (0.741 mL, 5.84 mmol), THF (58 mL) and diethyl [(phenylmethyl)thio ]propanedioate (1.00 g, 3.54 mmol). Melting point 170 °C with decomposition; 1H NMR (400 MHz, DMSO-d6) δ 3.98 (s, 2H), 6.13 (s, 2H), 6.61 (s, 1H), 7.05 (d, 2H), 7.27 (m, 7H). Example 65 6-[4-(Benzoyloxy)phenyl]-4-hydroxy-3-[(phenylmethyl)thio]-2H-pyran-2-one The title compound was prepared according to method A, using 4'-benzoyloxyacetophenone ( 1.27 g, 5.31 mmol) lithium bis(trimethylsilyl)amide (0.977 g, 5.84 mmol) chlorotrimethylsilane (0.741 mL, 5.84 mmol), THF (58 mL) and diethyl [(phenylmethyl)thio]propanedioate (1.00 g, 3.54 mmol) . Melting point 205 °C with decomposition; 1H NMR (400 MHz, DMSO-d6) δ 4.01 (s, 2H), 6.75 (s, IH), 7.21 (m, IH), 7.25 (d, 4H), 7.47 (d, 2H), 7.63 (t, 2H), 7.77 (t, 1H), 7.90 (d, 2H), 8.16 (d, 2H). ;Example 66 ;3-[Cyclohexyl(phenylthio)methyl]-4-hydroxy-6-phenyl-2H-pyran-2-one ;The title compound was prepared according to method C, using 4-hydroxy-6-phenyl-2H-pyran -2-one (1.00 g, 5.31 mmol), ethanol (10 mL), cyclohexanecarboxaldehyde (0.707 mL, 5.84 mmol), thiophenol (1.40 mL, 13.8 mmol), piperidine (0.5 mL), and acetic acid (0.5 mL). ;Example 67 ;4-Hydroxy-3-[(2-phenylethyl)thio]-6-[4-(phenylthio)phenyl]-2H-pyran-2-one ;The title compound was prepared according to method A, using 4'- (phenylthio)acetophenone (1.15 g, 5.06 mmol) lithium bis(trimethylsilyl)amide (0.930 g, 5.56 mmol) chlorotrimethylsilane (0.705 mL, 5.56 mmol), THF (56 mL) and diethyl [(2-phenylethyl)thio]propanedioate (1.00 g, 3.37 mmol). Melting point 120-121 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.76 (t, 2H), 2.98 (t, 2H), 6.72 (s, 1H), 7.24 (m, 7H), 7.45 (m, 5H), 7.74 (d, 2H). ;Example 68 ;4-Hydroxy-6-[4-[(2-methoxyphenyl)methoxy]phenyl]-3-[(2-phenylethyl)thio]-2H--pyran-2-one ;The title compound was prepared according to the method A, using 4'-[(2-methoxyphenyl)methoxy]-phenylacetophenone (1.29 g, 5.06 mmol) lithium bis(trimethylsilyl)amide (0.930 g, 5.56 mmol) chlorotrimethylsilane (0.705 mL, 5.56 mmol), THF (56 mL ) and diethyl [(2-phenylethyl)thio]propanedioate (1.00 g, 3.37 mmol). Melting point 138-139 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.77 (t, 2H), 2.98 (t, 2H), 3.83 (s, 3H), 5.14 (s, 2H), 6.68 (s, 1H), 6.97 (t, IH), 7.08 (s, IH), 7.20 (m, 7H), 7.53 (t, IH), 7.40 (d, IH), 7.76 (d, 2H), 11.85 (broad s, IH). Example 69 4-Hydroxy-6-[4-[(2-methoxyphenyl)methoxy]-3-methylphenyl]-3-[(2-phenylethyl)-thio]-2H-pyran-2-one Title compound was prepared according to method A, using 4'-[(2-methoxyphenyl)methoxy]-3'-methylacetophenone (1.36 g, 5.06 mmol) lithium bis(trimethylsilyl)amide (0.930 g, 5.56 mmol) chlorotrimethylsilane (0.705 mL, 5.56 mmol), THF (56 mL) and diethyl f(2-phenylethyl)thio]-propanedioate (1.00 g, 3.37 mmol). Melting point 170 °C with decomposition; 1H NMR (400 MHz, DMSO-d6) δ 2.25 (s, 3H), 2.77 (t, 2H), 2.97 (t, 2H), 3.84 (s, 3H), 5.17 (s, 2H), 6.67 (s, IH), 6.98 (i, IH), 7.70 (d, IH,-, 7.27 (m, 6H), 7.41 (t, IH), 7.43 (d, IH), 7.65 (m, 2H), 11.81 (broad s , IH).; Example 70; 6-(3,5-Dimethylphenyl)-4-hydroxy-3-[(phenylmethyl)thio]-2H-pyran-2-one; The title compound was prepared according to method A, using 3! ,5'-dimethylacetophenone (0.785 g, 5.31 mmol) lithium-bis(trimethylsilyl)amide (0.977 g, 5.84 mmol) chlorotrimethylsilane (0.741 mL, 5.84 mmol), THF (58 mL) and diethyl [(phenyl-ethyl)thio] propanedioate (1.00 g, 3.54 mmol). Melting point 170 °C with decomposition; 1H NMR (400 MHz, DMSO-d6) δ 2.33 (s, 6H), 3.99 (s, 2H), 6.67 (s, IH), 7.21 ( m, 6H), 7.39 (s, 2H). ;Example 71 ;4-Hydroxy-6-[4-[(2-methoxyphenyl)methoxy]]-3-[(2-phenylethyl)thio]-2H-pyran- -2-one; The title compound was prepared according to method A, using 4'-phenoxyacetophenone (1.07 g, 5.06 mmol) lithium bis(trimethylsilyl)amide (0.930 g, 5.56 mmol) chlorotrimethylsilane (0.705 mL, 5.56 mmol), THF ( 56 mL) and diethyl [(2-phen ylethyl)thio]propanedioate (1.00 g, 3.37 mmol). Melting point 127-128 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.77 (t, 2H), 2.99 (t, 2H), 6.72 (s, 1H), 7.18 (m, 10H), 7.46 (t, 2H), 7.82 (d, 2H). ;Example 72 ;4-Hydroxy-6-phenyl-3-[[[4-(phenylmethoxy)phenyl]methyI]thio]-2H-pyran-2-one ;The title compound was prepared according to method B, using 4-hydroxy- 6-phenyl-2H-pyran-2-one (0.500 g, 2.65 mmol), ethanol (7 mL), 1M sodium hydroxide (2.65 mL) and [4-(phenyl-methoxy)phenyl]methyl-p-toluenethiosulfonate ( 1.01 g, 2.65 mmol). Melting point 185-186 °C; 1H NMR (400 MHz, DMSO-d6) δ 3.94 (s, 2H), 5.03 (s, 2H), 6.72 (s, 1H), 6.89 (d, 2H), 7.18 (d, 2H), 7.34 (m, 5H), 7.46 (m, 3H), 7.80 (m, 3H). ;Example 73 ;4-Hydroxy-3-[(2-phenylethyl)thio]-6-[4-[(2-pyridinylmethoxy)phenyl]-2H-pyran--2-one ;The title compound was prepared according to method A, using 4'-(2-pyridinylmethoxy)acetophenone (1.14 g, 5.06 mmol) lithium bis(trimethylsilyl)amide (0.930 g, 5.56 mmol) chlorotrimethylsilane (0.705 mL, 5.56 mmol), THF (56 mL) and diethyl [(2 -phenylethyl)thio]propanedioate (1.00 g, 3.37 mmol). Melting point 179 °C with decomposition; 1H NMR (400 MHz, DMSO-d6) δ 2.77 (t, 2H), 2.98 (t, 2H), 5.27 (s, 2H), 6.68 (s, IH), 7.22 (m, 7H), 7.36 (m, IH), 7.53 (d, IH), 7.77 (d, 2H), 7.85 (t, IH), 8.60 (d, 2H), 11.88 (broad s, IH). ;Example 74 ;Ethyl 4-[4-hydroxy-2-oxo-3-[(2-phenylethyl)thio]-2H-pyran-6-yl]phenoxyacetate ;To a methanol solution (3 mL) of 4-hydroxy-6-( To 4-hydroxyphenyl)-3-[(2-phenylethyl)thio-2H-pyran-2-one (0.500 g, 1.47 mmol) was added cesium carbonate (0.955 g, 2.94 mmol). The reaction was stirred for 3 hours and concentrated in vacuo. After that, dimethylformamide (15 mL) was added, and the residue was evaporated to dryness under vacuum. The solid was dissolved in dimethylformamide (3 mL) and bromomethylacetate (0.491 mL, 2.94 mmol) was added. The mixture was stirred for 3 hours. The reaction was "quenched" by diluting with ethyl acetate (100 mL). The organic layer was washed with 1M HCl, then with water, and with saturated sodium chloride solution, after which it was dried over anhydrous magnesium sulfate. After evaporation of the solvent in vacuo, the crude product was purified by column chromatography (SiO2 230-400 mesh) using a gradient from 15% ethyl acetate/hexane to 50% ethyl acetate/hexane to 30% ethyl acetate/30% hexane/40% methylene chloride. Melting point 169-171 °C; 1H NMR (400 MHz, DMSO-d6) δ 1.20 (t, 3H), 2.75 (t, 2H), 2.96 (t, 2H), 4.16 (q, 2H), 4.87 (s, 2H), 6.69 (s, IH), 7.06 (d, 2H), 7.19 (m, 5H), 7.73 (d, 2H), 11.85 (broad s, IH). ;Example 75 ;4-Hydroxy-3-[2-naphthalenyl(phenylthio)methyl]-6-phenyl-2H-pyran-2-one ;The title compound was prepared according to method C, using 4-hydroxy-6-phenyl-2H -pyran-2-one (1.00 g, 5.31 mmol), ethanol (10 mL), 2-naphthalaldehyde (0.912 g, 5.84 mmol), thiophenol (1.40 mL, 13.8 mmol), piperidine (0.5 mL), and acetic acid ( 0.5 mL). Melting point 98-101 °C; 1H NMR (400 MHz, DMSO-d6) δ 5.96 (s, IH), 6.73 (s, IH), 7.18 (t, IH), 7.36 (m, 4H), 7.52 (m, 5H), 7.88 (m, 3H), 8.07 (s, 1H). ;Example 76 ;4-Hydroxy-3-[2-naphthalenylthio)phenylenethyI]-6-phenyl-2H-pyran-2-one ;The title compound was prepared according to method C, using 4-hydroxy-6-phenyl-2H-pyran -2-one (1.00 g, 5.31 mmol), ethanol (10 mL), benzaldehyde (0.593 g, 5.84 mmol), 2-naphthalenethiol (2.21 mL, 13.8 mmol), piperidine (0.5 mL), and acetic acid (0.5 mL ). Melting point 200 °C with decomposition; 1H NMR (400 MHz, DMSO-d6) δ 5.9 (s, IH), 6.71 (s, IH), 7.20 (m, 5H), 7.44 (m, 7H), 7.75 (m, 3H), 7.82 (m, 2H). ;Example 77 ;4-[4-hydroxy-2-oxo-3-[(2-phenylethyl)thio]-2H-pyran-6-yl]phenoxyacetic acid ;Solutions of ethyl 4-[4-hydroxy-2-oxo- 1M sodium hydroxide (2.34 mmol) was added to 3-[(2-phenylethyl)thio]-2H-pyran-6-yl]phenoxyacetate (0.939 mmol) in tetrahydrofuran (10 mL). The reaction was stirred for 5 hours, and was "quenched" with an additional amount of water (10 mL), followed by acidification of conc. HCl to pH 2. The aqueous layer was then extracted 2x with ethyl acetate (100 mL). The combined organic extracts were washed with saturated sodium chloride solution and dried over anhydrous magnesium sulfate. After evaporation of the solvent in vacuo, the crude product was purified by column chromatography (silica gel 230-400 mesh) using 94/5/1 methylene chloride/methanol/acetic acid as eluent. Melting point 182-183 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.76 (t, 2H), 2.97 (t, 2H), 4.78 (s, 2H), 6.67 (s, 1H), 7.06 (d, 2H), 7.21 (m, 5H), 7.75 (d, 2H). ;Example 78 ;4-Hydroxy-3-[(2-phenylethyl)thio]-6-[4-[(3-pyridinylmethoxy)phenyl]-2H-pyran--2-one ;The title compound was prepared according to method A, using 4'-(3-pyridinylmethoxy)acetophenone (1.14 g, 5.06 mmol) lithium bis(trimethylsilyl)amide (0.930 g, 5.56 mmol) chlorotrimethylsilane (0.705 mL, 5.56 mmol), THF (56 mL) and diethyl [(2 -phenylethyl)thio]propanedioate (1.00 g, 3.37 mmol). Melting point 178-179 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.76 (t, 2H), 2.98 (t, 2H), 5.25 (s, 2H), 6.69 (s, IH), 7.21 (m, 7H), 7.45 (q, IH), 7.77 (d, 2H), 7.91 (d, IH), 8.57 (broad s, IH), 8.70 (broad s, IH). ;Example 79 ;6-[4-[(Cyclohexylmethoxy)phenyl]-4-hydroxy-3-[(2-phenylethyl)thio]-2H-pyran-2-one ;The title compound was prepared according to method A, using 4' -(cyclohexylmethoxy)acetophenone (2.50 g, 10.77 mmol) lithium bis(trimethylsilyl)amide (2.70 g, 16.16 mmol) chlorotrimethylsilane (2.05 mL, 16.16 mmol), THF (107 mL) and diethyl [(2-phenylethyl)thio] propanedioate (1.00 g, 3.37 mmol). Melting point 130-132 °C; 1H NMR (400 MHz, DMSO-d6) δ 1.15 (m, 5H), 1.81 (m, 6H), 2.77 (t, 2H), 2.97 (t, 2H), 3.85 (d, 2H), 6.67 (s, 1H), 7.21 (m, 5H), 7.45 (q, 1H), 7.74 (d, 2H). ;Example 80 ;4-Hydroxy-3-[(2-phenylethyl)thio]-6-[4-(phenylsulfonyl)phenyl]-2H-pyran-2-one ;The title compound was prepared according to method A, using 4'- (phenylsulfonyl)acetophenone (2.50 g, 9.61 mmol) lithium bis(trimethylsilyl)amide (2.41 g, 14.42 mmol) chlorotrimethylsilane (1.82 mL, 14.42 mmol), THF (96 mL) and diethyl [(2-phenylethyl)thio]propanedioate (1.00 g, 3.37 mmol). Melting point 194-195 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.76 (t, 2H), 3.01 (t, 2H), 6.87 (s, 1H), 7.19 (m, 5H), 7.68 (m, 3H), 8.04 (m, 6H), 12.05 (wide s, IH). ;Example 81 ;4-Hydroxy-3-[(2-phenylethyl)thio]-6-[4-(benzoyloxy)phenyl]-2H-pyran-2-one ;The title compound was prepared according to method A, using 4'- benzoyloxyacetophenone (2.50 g, 10.41 mmol) lithium bis(trimethylsilyl)amide (2.61 g, 15.62 mmol) chlorotrimethylsilane (1.98 mL, 15.62 mmol), THF (100 mL) and diethyl [(2-phenylethyl)thio]propanedioate (1.00 g) , 3.37 mmol). Melting point 164-166 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.78 (t, 2H), 3.01 (t, 2H), 6.81 (s, 1H), 7.21 (m, 5H), 7.49 (d, 2H), 7.63 (t, 2H), 7.77 (t, IH), 7.92 (d, 2H), 12.00 (broad s, IH). ;Example 82 ;4-Hydroxy-3-[(2-phenylethyl)thio]-6-[4-[(phenylsulfinyl)phenyl]-2H-pyran-2-one ;The title compound was prepared according to method A, using 4' -(phenylsulfinyl)acetophenone (2.50 g, 10.24 mmol) lithium bis(trimethylsilyl)amide (2.57 g, 15.36 mmol) chlorotrimethylsilane (1.94 mL, 15.36 mmol), THF (100 mL) and diethyl [(2-phenylethyl)thio] propanedioate (1.00 g, 3.37 mmol). Melting point 171-173 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.76 (t, 2H), 3.01 (t, 2H), 6.83 (s, 1H), 7.19 (m, 5H), 7.54 (m, 3H), 7.75 (d, 2H), 7.86 (d, 2H), 7.95 (d, 2H), 12.05 (broad s, 1H). Example 83 4-Hydroxy-3-[(2-phenylethyl)thio]-6-(4-pyridinyl)-2H-pyran-2-one The title compound was prepared according to method A, using 4-acetylpyridine (2.50 g , 20.63 mmol) lithium bis(trimethylsilyl)amide (5.17 g, 30.94 mmol) chlorotrimethylsilane (3.92 mL, 30.94 mmol), THF (200 mL) and diethyl [(2-phenylethyl)thio]propanedioate (1.00 g, 3.37 mmol) . Melting point 149-152 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.78 (t, 2H), 3.04 (t, 2H), 6.98 (s, 1H), 7.20 (m, 5H), 7.74 (d, 2H), 8.74 (d, 2H). ;Example 84 ;3-[1,4-Bis(phenylthio)butyl]-4-hydroxy-6-phenyl-2H-pyran-2-one (+/-) ;The title compound was prepared according to method C, using 4- hydroxy-6-phenyl-2H-pyran-2-one (1.00 g, 5.31 mmol), ethanol (10 mL), cyclopropylcarboxaldehyde (0.436 g, 5.84 mmol), thiophenol (1.40 mL, 13.8 mmol), piperidine (0.5 mL) , and acetic acid (0.5 mL). Melting point 75-77 °C; 1H NMR (400 MHz, DMSO-d6) δ 5.9 (s, 1H), 6.71 (s, 1H), 7.20 (m, 5H), 7.44 (m, 7H), 7.75 (m, 3H), 7.82 (m, 2H). ;Example 85 ;4-Hydroxy-6-phenyl-3-[phenyl[phenylmethyl)thio]methyl]-2H-pyran-2-one (+/-) ;The title compound was prepared according to method C, using 4-hydroxy- 6-phenyl-2H-pyran-2-one (1.00 g, 5.31 mmol), ethanol (10 mL), benzaldehyde (0.593 mL, 5.84 mmol), benzyl mercaptan (1.62 mL, 13.8 mmol), piperidine (0.5 mL), and acetic acid (0.5 mL). Melting point 189-191 °C; 1H NMR (400 MHz, DMSO-d6) δ 3.70 (dd, 2H), 5.29 (s, IH), 6.65 (s, IH), 7.23 (m, 8H), 7.50 (m, 5H), 7.73 (m, 2H), 11.96 (wide s, IH). ;Example 86 ;4-Hydroxy-3-[[(2-methoxyphenyl)thio]phenylmethyl]-6-phenyl-2H-pyran-2-one (+/-) ;The title compound was prepared according to method C, using 4- hydroxy-6-phenyl-2H-pyran-2-one (1.00 g, 5.31 mmol), ethanol (10 mL), benzaldehyde (0.593 mL, 5.84 mmol), methoxythiophenol (1.93 mL, 13.8 mmol), piperidine (0.5 mL) , and acetic acid (0.5 mL). Melting point 165-170 °C; 1H NMR (400 MHz, DMSO-d6) δ 3.87 (s, 3H), 5.81 (s, IH), 6.70 (s, IH), 6.84 (t, IH), 7.19 (m, 3H), 7.28 (t, 2H), 7.53 (m, 3H), 7.75 (m, 2H), 12.13 (broad s, 1H). ;Example 87 ;4-Hydroxy-3-[3-methyl-1-(phenylthio)butyl]-6-phenyl-2H-pyran-2-one (+/-) ;The title compound was prepared according to method C, using 4 -hydroxy-6-phenyl-2H-pyran-2-one (1.00 g, 5.31 mmol), ethanol (10 mL), isovaleraldehyde (0.626 mL, 5.84 mmol), thiophenol (1.40 mL, 13.8 mmol), piperidine (0.5 mL ), and acetic acid (0.5 mL). Melting point 154-156 °C; 1H NMR (400 MHz, DMSO-d6) δ 0.89 (d, 3H), 0.93 (d, 3H), 1.63 (m, IH), 1.80 (m, IH), 2.32 (m, 2H), 4.82 (dd, 2H), 6.70 (s, IH), 7.24 (m, 3H), 7.82 (m, 2H), 10.49 (broad s, IH). ;Example 88 ;3-[2-Cyclohexyl-1-(phenylthio)ethyl]-4-hydroxy-6-phenyl-2H-pyran-2-one (+/-) ;The title compound was prepared according to method C, using 4 -hydroxy-6-phenyl-2H-pyran-2-one (1.00 g, 5.31 mmol), ethanol (10 mL), cyclohexylmethyl-carboxaldehyde (0.735 mL, 5.84 mmol), thiophenol (1.40 mL, 13.8 mmol), piperidine ( 0.5 mL), and acetic acid (0.5 mL). Melting point 205 °C with decomposition; 1H NMR (400 MHz, DMSO-d6) δ 0.91 (d, 3H), 1.25 (m, 5H), 1.73 (m, 5H), 2.58 (m, IH), 4.83 (dd, IH), 6.69 (s, 1H), 7.22 (m, 3H), 7.48 (m, 5H), 7.82 (ni, 2H). ;Example 89 ;4-Hydroxy-6-(3-phenoxyphenyl)-3-[(2-phenylethyl)thio]-2H-pyran-2-one ;The title compound was prepared according to method A, using 3'-phenoxyacetophenone (2.00 g, 9.43 mmol) lithium bis(trimethylsilyl)amide (2.36 g, 14.15 mmol) chlorotrimethylsilane (1.79 mL, 14.15 mmol), THF (100 mL) and diethyl [(2-phenylethyl)thio]propanedioate (1.00 g, 3.37 mmol) ). Melting point 114-115 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.76 (t, 2H), 2.99 (t, 2H), 6.76 (s, IH), 7.09 (m, 7H), 7.34 (s, IH), 7.44 (t, 2H), 7.56 (m, 2H). ;Example 90 ;4-Hydroxy-6-[3-methoxy-4-(phenylmethoxy)phenyl]-3-[(2-phenylethyl)thio]-2H-pyran-2-one ;The title compound was prepared according to method A, using 4'-benzoyloxy-3'-methoxy-acetophenone (2.00 g, 7.81 mmol) lithium bis(trimethylsilyl)amide (1.96 g, 11.71 mmol) chlorotrimethylsilane (1.48 mL, 11.71 mmol), THF (80 mL) and diethyl [ (2-phenylethyl)thio]propanedioate (1.00 g, 3.37 mmol). Melting point 114-115 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.77 (t, 2H), 2.98 (t, 2H), 3.86 (s, IH), 6.75 (s, IH), 7.21 (m, 7H), 7.40 (m, 6H). ;Example 91 ;6-(3,5-Dimethylphenyl)-4-hydroxy-3-[(2-phenylethyl)thio]-2H-pyran-2-one ;The title compound was prepared according to method A, using 3',5 '-dimethylacetophenone (1.75 g, 11.82 mmol) lithium bis(trimethylsilyl)amide (1.89 g, 17.73 mmol) chlorotrimethylsilane (2.25 mL, 17.73 mmol), THF (120 mL) and diethyl [(2-phenylethyl)thio]propanedioate ( 1.00 g, 3.37 mmol). Melting point 155-157 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.34 (s, 6H), 2.77 (t, 2H), 2.99 (t, 2H), 6.72 (s, 1H), 7.21 (m, 6H), 7.41 (s, 2H), 8.74 (d, 2H). ;Example 92 ;4-Hydroxy-3-[[(3-methoxyphenyl)methyl]thio]-6-phenyl-2H-pyran-2-one ;The title compound was prepared according to method B, using 4-hydroxy-6-phenyl -2H-pyran-2-one (1.00 g, 5.31 mmol), ethanol (15 mL), 1M sodium hydroxide (5.31 mL) and [3-(methoxy)-phenyl]methyl-p-toluenethiosulfonate (2.12 g, 6.90 mmol). Melting point 134-136 °C; 1H NMR (400MHz, DMSO-d6) δ 3.69 (s, 3H), 3.99 (s, 2H), 6.75 (m, 2H), 6.83 (m, 2H), 7.16 (t, 1H), 7.53 (m, 3H ), 7.79 (m, 2H). ;Example 93 ;4-Hydroxy-3-[4-methyl-1-(phenylthio)pentyl]-6-phenyl-2H-pyran-2-one (+/-) ;The title compound was prepared according to method C, using 4 -hydroxy-6-phenyl-2H-pyran-2-one (1.00 g, 5.31 mmol), ethanol (10 mL), 4-methylpentanal (0.584 mL, 5.84 mmol), thiophenol (1.40 mL, 13.8 mmol), piperidine ( 0.5 mL), and acetic acid (0.5 mL). Melting point 144-145 °C; 1H NMR (400 MHz, DMSO-d6) δ 0.80 (d, 3H), 0.81 (d, 3H), 1.07 (m, IH), 1.18 (m, IH), 1.49 (m, IH), 1.89 (m, IH), 2.19 (m, IH), 4.51 (dd, IH), 6.68 (s, IH), 7.19 (t, IH), 7.29 (t, 2H), 7.35 (d, 2H), 7.53 (m, 3H ), 7.76 (m, 2H). ;Example 94 ;4-Hydroxy-6-phenyl-3-[[[3-(phenylmethoxy)phenyl]methyl]thio]-2H-pyran-2-one ;The title compound was prepared according to method B, using 4-hydroxy- 6-phenyl-2H-pyran-2-one (1.00 g, 5.31 mmol), ethanol (15 mL), 1M sodium hydroxide (5.31 mL) and [3-benzoyl)-phenyl]methyl-p-toluenethiosulfonate (2.65 g , 6.90 mmol). Melting point 140-141 °C; 1H NMR (400 MHz, DMSO-d6) δ 3.98 (s, 2H), 5.01 (s, 2H), 6.75 (s, IH), 6.83 (m, 2H), 6.91 (m, IH), 7.28 (t, 1H), 7.34 (m, 4H), 7.52 (m, 3H), 7.80 (m, 2H). ;Example 95 ;3-[(1,3-Benzodioxol-5-ylmethyl)thio]-4-hydroxy-6-phenyl-2H-pyran-2-one ;The title compound was prepared according to method B, using 4-hydroxy- 6-phenyl-2H-pyran-2-one (1.00 g, 5.31 mmol), ethanol (15 mL), 1 M sodium hydroxide (5.31 mL) and [1,3-benzo-dioxol-5-ylmethyl-p- toluenethiosulfonate (2.22 g, 6.90 mmol). Melting point 162-164 °C; 1H NMR (400 MHz, DMSO-d6) δ 3.92 (s, 2H), 5.95 (s, 2H), 6.75 (m, 4H), 7.53 (m, 3H), 7.79 (m, 2H). ;Example 96 ;4-Hydroxy-3-[[(2-methoxyphenyl)methyl]]-6-phenyl-2H-pyran-2-one ;The title compound was prepared according to method B, using 4-hydroxy-6-phenyl- 2H-pyran-2-one (0.500 g, 2.65 mmol), ethanol (7 mL), 1 M sodium hydroxide (2.65 mL) and [(2-methoxy-phenyl)methyl]-p-toluenethiosulfonate (0.816 g, 2.65 mmol). Melting point 152-153 °C; 1H NMR (400 MHz, DMSO-d6) δ 3.73 (s, 3H), 3.95 (s, 2H), 6.71 (s, IH), 6.81 (t, IH), 6.91 (d, IH), 7.13 (d, 1H), 7.17 (t, 1H), 7.53 (m, 3H), 7.79 (m, 2H). ;Example 97 ;4-Hydroxy-3-[[(2-methylphenyl)methyl]]-6-phenyl-2H-pyran-2-one ;The title compound was prepared according to method B, using 4-hydroxy-6-phenyl- 2H-pyran-2-one (1.00 g, 5.31 mmol), ethanol (15 mL), 1M sodium hydroxide (5.31 mL) and [(2-methylphenyl)-methyl]-p-toluenethiosulfonate (1.55 g, 5.31 mmol) . Melting point 176-178 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.42 (s, 3H), 3.99 (s, 2H), 6.74 (s, 1H), 7.09 (m, 4H), 7.53 (m, 3H), 7.79 (m, 2H). ;Example 98 ;4-Hydroxy-3-[[(3-methylphenyl)methyl]thio]-6-phenyl-2H-pyran-2-one ;The title compound was prepared according to method B, using 4-hydroxy-6-phenyl -2H-pyran-2-one (1.00 g, 5.31 mmol), ethanol (15 mL), 1M sodium hydroxide (5.31 mL) and [(3-methylphenyl)-methyl]-p-toluenethiosulfonate (1.55 g, 5.31 mmol ). Melting point 139-140 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.23 (s, 3H), 3.96 (s, 2H), 6.74 (s, 1H), 7.07 (m, 4H), 7.53 (m, 3H), 7.79 (m, 2H). ;Example 99 ;4-Hydroxy-3-[[(4-methylphenyl)methyl]thio]-6-phenyl-2H-pyran-2-one ;The title compound was prepared according to method B, using 4-hydroxy-6-phenyl -2H-pyran-2-one (1.00 g, 5.31 mmol), ethanol (15 mL), 1M sodium hydroxide (5.31 mL) and [(4-methylphenyl)-methyl]-p-toluenethiosulfonate (1.55 g, 5.31 mmol ). Melting point 164-165 °C; 1H NMR (400MHz, DMSO-d6) δ 2.23 (s, 3H), 3.96 (s, 2H), 6.74 (s, 1H), 7.06 (d, 2H), 7.14 (d, 2H), 7.53 (m, 3H ), 7.79 (m, 2H). Example 100 6-[1,1'-Biphenyl]-3-yl-4-hydroxy-3-[(2-phenylethyl)thio]-2H-pyran-2-one The title compound was prepared according to method A, using 3'-phenylacetophenone (2.00 g, 10.21 mmol) trimethylsilyl triphthalate (2.36 mL, 12.24 mmol), triethylamine (2.84 mL, 20.40 mmol), methylene chloride (26 mL) and diethyl [(2-phenylethyl)thio]propanedioate (1.00 g, 3.37 mmol). Melting point 93-94 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.79 (t, 2H), 3.01 (t, 2H), 6.92 (s, IH), 7.21 (m, 5H), 7.42 (t, IH), 7.52 (t, 2H), 7.64 (t, 1H), 7.75 (d, 2H), 7.82 (t, 2H), 8.02 (s, 1H). ;Example 101 ;4-Hydroxy-3-[[(4-methoxyphenyl)methyl]thio]-6-phenyl-2H-pyran-2-one ;The title compound was prepared according to method B, using 4-hydroxy-6-phenyl -2H-pyran-2-one (1.00 g, 5.31 mmol), ethanol (15 mL), 1M sodium hydroxide (5.31 mL) and [(4-methoxy-phenyl)methyl]-p-toluenethiosulfonate (2.21 g, 6.90 mmol). Melting point 168-170 °C; 1H NMR (400 MHz, DMSO-d6) δ 3.96 (s, 3H), 3.95 (s, 2H), 6.73 (s, 1H), 6.81 (d, 2H), 7.17 (d, 2H), 7.53 (m, 3H), 7.79 (m, 2H). ;Example 102 ;3-[2-Cyclohexyl-1-(cyclohexylthio)ethyl]-4-hydroxy-6-phenyl-2H-pyran-2-one (+/-) ;The title compound was prepared according to method C, using 4 -hydroxy-6-phenyl-2H-pyran-2-one (1.00 g, 5.31 mmol), ethanol (10 mL), cyclohexylmethylcarboxaldehyde (0.735 mL, 5.84 mmol), cyclohexyl mercaptan (1.60 g, 13.8 mmol), piperidine (0.5 mL ), and acetic acid (0.5 mL). Melting point 220 °C with decomposition; 1H NMR (400 MHz, DMSO-d6) δ 0.86 (m, 2H), 1.18 (m, 9H), 1.66 (m, 10H), 2.03 (m, 2H), 2.58 (m, 2H), 4.25 (m, 1H), 6.68 (s, 1H), 7.53 (m, 3H), 7.75 (m, 2H). ;Example 103 ;3-[1-[(2,6-Dimethylphenyl)thio-3-methylbutyl]-4-hydroxy-6-phenyl-2H-pyran-2-one (+/-) ;The title compound was prepared by method C, using 4-hydroxy-6-phenyl-2H-pyran-2-one (1.00 g, 5.31 mmol), ethanol (10 mL), isovaleraldehyde (0.63 mL, 5.84 mmol), 2,6-di-methylthiophenol ( 1.90 g, 13.8 mmol), piperidine (0.5 mL), and acetic acid (0.5 mL). Melting point 166-167 °C; 1H NMR (400 MHz, DMSO-d6) δ 0.78 (d, 3H), 0.83 (d, 3H), 1.42 (m, IH), 1.47 (m, IH), 2.46 (m, IH), 2.51 (s, 6H), 4.37 (m, 1H), 6.51 (s, 1H), 7.70 (m, 3H), 7.52 (m, 3H), 7.74 (m, 2H). ;Example 104 ;3-[1-(Cyclohexylthio)-2-cyclopropylethyl]-4-hydroxy-6-phenyl-2H-pyran-2-one ;The title compound was prepared according to method C, using 4-hydroxy-6-phenyl -2H-pyran-2-one (1.00 g, 5.31 mmol), ethanol (10 mL), cyclopropylmethylcarboxaldehyde (0.67 mL, 5.84 mmol), cyclohexyl mercaptan (1.68 g, 13.8 mmol), piperidine (0.5 mL), and acetic acid ( 0.5 mL). Melting point 69-71 °C; 1H NMR (400 MHz, DMSO-d6) δ -0.02 (m, IH), 0.05 (m, IH), 0.34 (m, 2H), 0.64 (m, 2H), 1.22 (m, 5H), 1.52 (m , IH), 1.67 (m, 3H), 1.84 (m, IH), 1.97 (m, 2H), 2.64 (m, IH), 4.21 (t, IH), 6.69 (s, IH), 7.52 (m, 3H), 7.75 (m, 2H). ;Example 105 ;3-[1-[(2,6-Dichlorophenyl)thio]-3-methylbutyl]-4-hydroxy-6-phenyl-2H-pyran-2-one (+/-) ;The title compound was prepared according to method C, using 4-hydroxy-6-phenyl-2H-pyran-2-one (1.00 g, 5.31 mmol), ethanol (10 mL), isovaleraldehyde (0.63 mL, 5.84 mmol), 2,6-dichloro-phenol (2.74 g, 13.8 mmol), piperidine (0.5 mL), and acetic acid (0.5 mL). Melting point 158-162 °C; 1H NMR (400 MHz, DMSO-d6) δ 0.83 (d, 3H), 0.87 (d, 3H), 1.49 (m, IH), 1.74 (m, IH), 2.39 (m, IH), 4.68 (m, 1H), 6.77 (s, 1H), 7.49 (m, 5H), 7.74 (m, 3H). ;Example 106 ;3-[1-(Cyclohexylthio)-3,3-dimethylbutyl]-4-hydroxy-6-phenyl-2H-pyran-2-one (+/-) ;The title compound was prepared according to method C, using 4-hydroxy-6-phenyl-2H-pyran-2-one (1.00 g, 5.31 mmol), ethanol (10 mL), 3,3-dimethylbutanal (0.73 mL, 5.84 mmol), cyclohexyl mercaptan (1.86 g, 13.8 mmol) , piperidine (0.5 mL), and acetic acid (0.5 mL). Melting point >225 °C; 1H NMR (400 MHz, DMSO-d6) δ 0.85 (s, 9H), 1.25 (m, 5H), 1.65 (m, 7H), 4.30 (m, IH), 6.69 (s, IH), 7.54 (m, 3H), 7.75 (m, 2H). ;Example 107 ;Ethyl [4-[4-Hydroxy-2-oxo-3-[(2-phenylethyl)thio]-2H-pyran-6-yl]-2-methoxy-phenoxy]acetate ;The title compound was prepared by method A, using (4-acetyl-2-methylphenoxy) acetate (2.00 g, 8.47 mmol), trimethylsilyl triphthalate (3.92 mL, 20.33 mmol), triethylamine (4.72 mL, 33.88 mmol), methylene chloride (22 mL ) and diethyl [(2-phenylethyl)thio]propanedioate (1.00 g, 3.37 mmol). Melting point 154-156 °C; 1H NMR (400 MHz, DMSO-d6) δ 1.22 (t, 3H), 2.26 (s, 3H), 2.77 (t, 2H), 2.97 (t, 2H), 4.17 (t, 2H), 4.91 (s, 2H), 6.66 (s, 1H), 6.99 (d, 1H), 7.21 (m, 5H), 7.61 (m, 2H). Example 108 6-[3,5-Dimethyl-4-[[dimethyl(1,1-dimethylethyl)silyl]oxy]phenyl]-4-hydroxy-3-[(phenylmethyl)thio]-2H-pyran-2 -one ; The title compound was prepared according to method A, using 3',5'-dimethyl-4'-[[dimethyl(1,1-di-methylethyl)silyl]oxy]acetophenone (1.50 g, 5.39 mmol) trimethylsilyl triphthalate (1.24 mL, 6.47 mmol), triethylamine (1.50 mL, 10.78 mmol), methylene chloride (13 mL) and diethyl [(phenylmethyl)thio]propanedioate (1.00 g, 3.54 mmol). Melting point 137-139 °C; 1H NMR (400 MHz, DMSO-d6) δ 0.21 (s, 6H), 0.99 (s, 9H), 2.22 (s, 6H), 3.96 (s, 2H), 6.54 (s, IH), 6.99 (d, 1H), 7.21 (m, 5H), 7.44 (m, 2H). ;Example 109 ;4-Hydroxy-3-[(2-phenylethyl)thio]-6-[4-(4-pyridiniImethoxy)phenyl]-2H-pyran-2--one ;The title compound was prepared according to method A, using 4'-(4-pyridinylmethoxy)acetophenone (2.00 g, 8.81 mmol) trimethylsilyl triphthalate (2.04 mL, 10.57 mmol), triethylamine (2.45 mL, 17.62 mmol), methylene chloride (22 mL) and diethyl [(2-phenylethyl )thio]propanedioate (1.00 g, 3.37 mmol). Melting point 212 °C with decomposition; 1H NMR (400 MHz, DMSO-d6) δ 2.73 (t, 2H), 2.88 (t, 2H), 5.29 (s, 2H), 6.48 (s, IH), 7.18 (m, 5H), 7.45 (d, 2H), 7.74 (d, 2H), 8.90 (d, 2H). ;Example 110 ;3-[1-(Cyclopentylthio)-3-methylbutyl]-4-hydroxy-6-phenyl-2H-pyran-2-one (+/-) ;The title compound was prepared according to method C, using 4- hydroxy-6-phenyl-2H-pyran-2-one (1.00 g, 5.31 mmol), ethanol (10 mL), isovaleraldehyde (0.62 mL, 5.84 mmol), cyclopentyl mercaptan (1.43 g, 13.8 mmol), piperidine (0.5 mL) , and acetic acid (0.5 mL). Melting point 146-149 °C; 1H NMR (400 MHz, DMSO-d6) δ 0.85 (d, 2H), 0.87 (d, 2H), 1.32 (m, IH), 1.54 (m, 7H), 1.85 (m, IH), 2.00 (m, 2H), 3.04 (m, IH), 4.20 (dd, IH), 6.69 (s, IH), 7.53 (m, 3H), 7.76 (m, 2H), 11.69 (broad s, IH). ;Example 111 ;[4-[4-Hydroxy-2-oxo-3-[(2-phenylethyl)thio]-2H-pyran-6-yl]-2-methylphenoxy]-acetic acid ;Solutions of ethyl [4-[ 4-hydroxy-2-oxo-3-[(2-phenylethyl)thio]-2H-pyran-6-yl]-2-methylphenoxy]acetate (0.20 g, 0.45 mmol) in tetrahydrofuran (10 mL) was added 1 M sodium -hydroxy (1.13 mL, 1.13 mmol). The reaction was stirred for 5 hours, and was "quenched" with an additional amount of water (10 mL), and then the conc. HCl to pH 2. The aqueous layer was then extracted 2x with ethyl acetate (100 mL). The combined organic extracts were washed with saturated sodium chloride solution and dried over anhydrous magnesium sulfate. After evaporation of the solvent in vacuo, the crude product was purified by column chromatography (silica gel 230-400 mesh) using 94/5/1 methylene chloride/methanol/acetic acid as eluent. Melting point 210 °C with decomposition; 1H NMR (400 MHz, DMSO-d6) δ 2.26 (s, 3i I), 2.78 (t, 2H), 2.98 (t, 2H), 4.81 (s, 2H), 6.67 (s, IH), 6.97 (d , 2H), 7.21 (m, 5H), 7.61 (d, 2H). ;Example 112 ;3-[1-(Cyclohexylthio)-3-cyclopentylethyl]-4-hydroxy-6-phenyl-2H-pyran-2-one ;The title compound was prepared according to method C, using 4-hydroxy-6-phenyl -2H-pyran-2-one (1.00 g, 531 mmol), ethanol (10 mL), cyclopentylmethylcarboxaldehyde (0.65 mL, 5.84 mmol), cyclohexyl mercaptan (1.68 g, 13.8 mmol), piperidine (0.5 mL), and acetic acid ( 0.5 mL). Melting point 157-160 °C; 1H NMR (400 MHz, DMSO-d6) δ 1.44 (m, 18H), 2.01 (m, IH), 2.19 (m, IH), 2.60 (m, IH), 4.16 (m, IH), 6.68 (s, IH), 7.53 (m, 3H), 7.75 (m, 2H), 11.66 (broad s, IH). ;Example 113 ;4-Hydroxy-6-(4-hydroxy-3,5-dimethylphenyl)-3-[(phenylmethyl)thio]-2H-pyran-2--one ;Solutions of 6-[3,5-dimethyl] -4-[[dimethyl(1,1-dimethylethyl)silyl]osco]phenyl]-4-hydroxy-3-[(phenyl-methyl)thio]-2H-pyran-2-one in THF (10 mL) was added 3 M HCl (9.0 mL) at 0 °C. The reaction was stirred for 48 hours at room temperature. The reaction was "quenched" by pouring onto ethyl acetate and was washed with water, then saturated sodium chloride solution, and dried over anhydrous magnesium sulfate. After evaporation of the solvent in vacuo, the crude product was purified by column chromatography (SiO2 230-400 mesh) using 50% ethyl acetate/hexane. Melting point 174-176 °C; 1H NMR (250 MHz, DMSO-d6) δ 2.21 (s, 6H), 2.60 (m, IH), 3.96 (s, 2H), 6.52 (s, IH), 7.23 (s, 5H), 7.38 (s, 2H), 9.06 (s, 1H). ;Example 114 ;4-Hydroxy-6-phenyl-3-[[[3-(2-phenylethoxy)phenyl]methyl]thio]-2H-pyran-2-one ;The title compound was prepared according to method B, using 4- hydroxy-6-phenyl-2H-pyran-2-one (1.00 g, 5.31 mmol), ethanol (15 mL), 1M sodium hydroxide (5.31 mL) and [3-(2-(phenyl-ethoxy)phenyl]methyl -p-toluenethiosulfonate (2.11 g, 5.31 mmol). Melting point 85-90°C; 1H NMR (400 MHz, DMSO-d6) δ 2.96 (t, 2H), 3.96 (s, 2H), 4.09 (t, 2H) , 6.77 (m, 4H), 7.19 (m, 5H), 7.53 (m, 3H), 7.77 (m, 2H). ; Example 115 ; 4-Hydroxy-6-(4-(2-phenylethynyl)phenyl]- 3-[(2-phenylethyl)thio]-2H-pyran-2-one; The title compound was prepared according to method A, using 4'-(2-phenylethynyl)acetophenone (1.50 g, 6.81 mmol) trimethylsilyl triphthalate (1.57 mL , 8.17 mmol), triethylamine (1.89 mL, 13.62 mmol), methylene chloride (17 mL) and diethyl [(2-phenylethyl)thio]propanedioate (1.00 g, 3.37 mmol). Melting point 181-182 °C; 1H NMR ( 400 MHz, DMSO-d6) δ 2.78 (t, 2H), 3.02 (t, 2H), 6.85 (s, IH), 7.21 (m, 5H), 7.45 (m, 3H), 7.59 (d, 2H), 7.86 (d, 2H). ;Example 116 ;4-Hydroxy-6-[4-( 2-phenylethyl)phenyl]-3-[(2-phenylethyl)thio]-2H-pyran-2-one; The title compound was prepared according to method A, using 4'-(2-phenylethyl)acetophenone (1.50 g, 6.68 mmol ) trimethylsilyl triphthalate (1.55 mL, 8.02 mmol), triethylamine (1.86 mL, 13.36 mmol), methylene chloride (17 mL) and diethyl [(2-phenylethyl)thio]propanedioate (1.00 g, 3.37 mmol). Melting point 122-123 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.77 (t, 2H), 2.93 (m, 4H), 2.99 (t, 2H), 6.75 (s, 1H), 7.26 (m, 5H), 7.38 (d, 2H), 7.71 (d, 2H). ;Example 117 ;3-[1-(Cyclohexylthio)phenylmethyl]-4-hydroxy-6-phenyl-2H-pyran-2-one (+/-) ;The title compound was prepared according to method C, using 4-hydroxy-6 -phenyl-2H-pyran-2-one (1.00 g, 5.31 mmol), ethanol (10 mL), benzaldehyde (0.593 mL, 5.84 mmol), cyclohexyl mercaptan (1.68 g, 13.8 mmol), piperidine (0.5 mL), and acetic acid (0.5 mL). Melting point 189-191 °C; 1H NMR (400 MHz, DMSO-d6) δ 1.21 (m, 5H), 1.52 (m, IH), 1.91 (m, 2H), 2.58 (m, 2H), 5.37 (s, IH), 6.70 (s, IH), 7.17 (t, IH), 7.53 (m, 5H), 7.74 (m, 2H), 11.96 (broad s, IH). ;Example 118 ;4-Hydroxy-3-[(phenylmethyl)thio]-6-[3-(trifluoromethoxy)phenyl]-2H-pyran-2-one ;The title compound was prepared according to method A, using 3"-trifluoromethoxyacetophenone ( 3 g, 14.7 mmol) lithium bis(trimethylsilyl)amide (2.45 g, 14.7 mmol) chlorotrimethylsilane (2.47 mL, 14.7 mmol) and diethyl [(phenylmethyl)thio]propanedioate (1.00 g, 3.54 mmol). Melting point 128-132 ° C; 1H NMR (400 MHz, DMSO-d6) δ 4.03 (s, 2H), 6.81 (s, IH), 7.2 (m, 2H), 7.28 (m, 3H), 7.56 (dd, IH), 7.69 ( t, IH), 7.75 (s, IH), 7.86 (d, IH); IR (KBr) 2963, 1651, 1550, 1394, 1369, 1395, 1263, 1098, 1024, 800 cm 1; MS (CI) m /e 395 (M+H, 37), 309 (8), 273 (7), 205 (3), 119 (10); elemental analysis, calcd for C19H13O4SF3-H2O: C, 55.34; H, 3.67; found: C, 54.94; H, 4.03.; Example 119; 3-[(Cyclohexylmethyl)thio]-4-hydroxy-6-phenyl-2H-pyran-2-one; The title compound was prepared according to method B, using 4-hydroxy- 6-phenyl-2H-pyran-2-one (0.5 g, 2.66 mmol), ethanol (7 mL), 1 M sodium hydroxide (2.66 mL) and cyclohexylmethyl--p-toluenethiosulfo Nata (0.756 g, 2.66 mmol). Melting point 141-143°C; 1H NMR (400 MHz, DMSO-d6) δ 0.92 (m, 2H), 1.14 (m, 3H), 1.19 (m, 1H), 1.61 (m, 3H), L83 (m, 2H), 2.64 (d, 2H), 6.87 (s, 1H), 7.53 (m, 3H), 7.81 (m, 2H); IR (KBr) 3106, 2922, 1651, 1547, 1396, 1099, 766 cm 1 ; MS (CI) m/e 317 (M+H, 16), 279 (83), 242 (77), 201 (27), 177 (19), 134 (54), 105 (65), 98 (100) . ;Example 120 ;4-Hydroxy-3-[(2-phenylethyl)thio]-6-[3-methyl-4-(3-pyridinylmethoxy)phenyl]-2H--pyran-2-one ;The title compound was prepared by method A, using 3'-methyl-4'-(3-pyridinylmethoxy)-acetophenone (2.0 g, 8.29 mmol) lithium bis(trimethylsilyl)amide (1.53 g, 9.13 mmol) chlorotrimethylsilane (1.54 mL, 9.13 mmol) and diethyl [(2-phenylethyl)thio]propanedioate (1.00 g, 3.37 mmol). Melting point 149-151 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.25 (s, 3H), 2.78 (t, 2H), 2.97 (t, 2H), 5.28 (s, 2H), 6.69 (s, IH), 7.22 (m, 6H), 7.44 (dd, IH), 7.67 (s + d, 2H), 7.92 (d, IH), 8.58 (broad s, IH), 8.72 (broad s, IH); IR (KBr) 3430, 2926, 1713, 1626, 1505, 1263, 1136, 1028, 808, 705 cm 1; MS (CI) m/e 446 (M+H), 341 (15), 200 (6), 105 (100); Elemental analysis, calculated for C26H23O4SN: C, 70.09; H, 5.20; N, 3.14; found: C, 70.31; H, 5.27; N, 2.95. Example 121 6-(2,3-Dihydro-1,4-benzodioxin-6-yl)-4-hydroxy-3-[(phenylmethyl)thio]-2H-pyran-2-one The title compound was prepared by method A, using 1,4-benzodioxin-6-yl-methyl ketone (2.5 g, 14.25 mmol) lithium bis(trimethylsilyl)amide (2.35 g, 14.25 mmol) chlorotrimethylsilane (2.47 mL, 14.25 mmol) and diethyl [( phenylmethyl)thio]propanedioate (1.00 g, 3.55 mmol). Melting point 192-193 °C; 1H NMR (400 MHz, DMSO-d6) δ 3.99 (s, 2H), 4.17 (m, 4H), 6.8 (s, IH), 7.0 (d, IH), 7.2 (m, IH), 2.28 (m, 7H); IR (KBr) 3435, 2924, 1649, 1624, 1508, 1288, 1066, 698 cm 1 ; MS (CI) m/e 369 (M+H), 277 (12), 233 (12), 163 (9), 107 (10), 91 (76); Elemental analysis, calculated for C20H16O5S: C, 65.21; H, 4.38; found: C, 64.80; H, 4.17. ;Example 122 4-Hydroxy-3-[(2-phenylethyl)thio]-6-[3-(trifluoromethyl)phenyl]-2H-pyran-2-one ;The title compound was prepared according to method A, using the appropriate trimethylsilylenol ether (4.5 mmol) and diethyl [(2-phenylethyl)thio]propanedioate (1.33 g, 4.55 mmol). Melting point 117-118 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.8 (t, 2H), 3.03 (t, 2H), 6.94 (s, IH), 7.2 (m, 5H), 7.8 (t, IH), 7.94 (t, IH), 8.08 (s, IH), 8.14 (d, IH); IR (KBr) 3435, 3026, 2924, 1720, 1635, 1543, 1327, 1171, 1130, 696 cm 1 ; MS (Cl) m/e 393 (M+H, 100), 373 (9), 288 (38), 256 (20), 224 (11), 105 (62); Elemental analysis, calculated for C20H15SO3F3-H2O: C, 59.53; H, 4.18; found: C, 59.28; H, 3.81. ;Example 123 4-Hydroxy-3-[(phenylmethyl)thio]-6-[3-(trifluoromethyl)phenyl]-2H-pyran-2-one ;The title compound was prepared according to method A, using the appropriate trimethylsilylenol ether (9.8 mmol) and diethyl [(phenylmethyl)thio]propanedioate (2.76 g, 9.88 mmol). Melting point 152-153 °C; 1H NMR (400 MHz, DMSO-d6) δ 3.97 (s, 2H), 6.53 (s, IH), 7.25 (m, 5H), 7.61 (t, IH), 7.75 (d, IH), 8.03 (d, IH), 8.08 (s, IH); IR (KBr) 3434, 3244, 1678, 1628, 1535, 1522, 1435, 1341, 1316, 1192, 1132, 936, 706 cm l; MS (CI) m/e 379 (M+H), 257 (1), 91 (100); Elemental analysis, calculated for C29H13O3SF3: C, 60.31; H, 3.46; found: C, 60.53; H, 3.57. ;Example 124 ;4-Hydroxy-3-[(phenylmethyl)thio]-6-(2,3,4-trimethoxyphenyl)-2H-pyran-2-one ;The title compound was prepared according to method A, using 2'3l, 4'-trimethoxyacetophenone (1.5 g, 7.13 mmol) lithium bis(trimethylsilyl)amide (1.43 g, 8.56 mmol) chloromethylsilane (1.8 mL, 10.67 mmol) and diethyl [(phenylmethyl)thio]propanedioate (1.00 g, 3.54 mmol). ;Example 125 ;N-[4-[4-Hydroxy-2-oxo-3-[(2-phenylethyl)thio]-2H-pyran-6-yl]phenyl]-benzenesulfonamide ;The title compound was prepared by the method A, using the appropriate benzenesulfonamide (3.0 g, 10.91 mmol), lithium bis(trimethylsilyl)amide (3.65 g, 21.82 mmol) chlorotrimethylsilane (3.68 mL, 21.82 mmol) and diethyl [(2-phenylethyl)thio]propanedioate (1.00 g, 3.37 mmol). Melting point 89-91 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.78 (t, 2H), 3.03 (t, 2H), 6.86 (s, 1H), 7.25 (m, 6H), 7.72 (t, 3H), 7.86 (m, 5H); IR (KBr) 3443, 3335, 1725, 1632, 1543, 1383, 1171, 912, 729, 581, 552 cm 1 ; Elemental analysis, calculated for C25H21S2O5-H2O: C, 60.35; H, 4.66; N, 2.81; found: C, 60.13; H, 4.47; N, 3.23. ;Example 126 ;6-[4-(3,5-Dimethyl-4-isoxazolyl)methoxy]phenyl]-4-hydroxy-3-[(2-phenylethyl)-thio]-2H-pyran-2-one ;Title the compound was prepared according to method A, using 4'-(3,5-dimethyl-4-isoxazolyl)-acetophenone (1.65 g, 6.74 mmol) lithium bis(trimethylsilyl)amide (1.13 g, 6.74 mmol) chlorotrimethylsilane (1.14 mL, 6.74 mmol) and diethyl [(2-phenylethyl)thio]propanedioate (1.00 g, 3.37 mmol). Melting point 152-154 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.22 (s, 3H), 2.31 (s, 3H), 2.78 (t, 2H), 2.99 (t, 2H), 5.03 (s, 2H), 6.69 (s, 1H), 7.17 (d, 3H), 7.25 (m, 4H), 7.78 (d, 2H); IR (KBr) 2936, 2979, 1640, 1510, 1406, 1182, 988, 820, 764 cm 1; MS (CI) m/e 450 (M+H), 341 (10), 236 ), 112 (76), 105 (100); Elemental analysis, calculated for C25H23NO5S: C, 66.80; H, 5.16; N, 3.12; found: C, 66.42; H, 5.20; N, 2.74. ;Example 127 ;3-[(Cyclohexylthio)phenylmethyl]-4-hydroxy-6-[3-methyl-4-(3-pyridinyl-methoxy)phenyl]-2H-pyran-2-one (+/-) ;Title the compound was prepared according to method C, using 4-hydroxy-6-[3-methyl-4-(3-pyridin-ylmethoxy)phenyl]-2H-pyran-2-one (0.5 g, 1.62 mmol), benzaldehyde (0.189 g , 1.78 mmol), cyclohexyl mercaptan (0.489 g, 4.212 mmol), piperidine (0.5 mL), and acetic acid (0.5 mL). Melting point 84-85 °C; IR (KBr) 3059, 2930, 2853, 1676, 1601, 1449, 1260, 1134, 700 cm 1; MS (CI) m/e 446 (2), 331 (9), 226 (61), 205 (24), 135 (44). ;Example 128 ;Methyl 2-[[(4-hydroxy-2-oxo-6-phenyl-2H-pyran-3-yl]thio]methyl]benzoate ;The title compound was prepared according to method B, using 4-hydroxy-6 -phenyl-2H-pyran-2-one (2.0 g, 10.63 mmol), [2-(carbomethoxy)phenyl]methyl-p-toluenethiosulfonate (3.57 g, 10.63 mmol), 1M NaOH (10.63 mL) and ethanol (20 mL ).M.P. 122-123 °C; 1H NMR (400 MHz, DMSO-d6) δ 3.81 (s, 3H), 4.31 (s, 2H), 6.67 (s, IH), 7.25 (d, IH), 7.31 ( t, IH), 7.44 (t, IH), 7.44 (m, 3H), 7.53 (d, IH), 7.99 (m, 3H); IR (KBr) 3005, 2951, 1721, 1653, 1543, 1400, 1267 , 1078, 966, 766, 711, 520 cm 1; MS (CI) m/e 397 (M+29, 4), 369 ((M+H), 40), 337 (34), 191 (26), 149 (100), 105 (14). ; Example 129 ; 3-[1-(Cyclohexylylthio)-3-methylbutyl]-6-(2,3-dihydro-1,4-benzodioxin-6-yl)-4- hydroxy-2H-pyran-2-one (+/-) ; The title compound was prepared according to method C, using 4-hydroxy-6-[1,4-benzodioxin-6--yl]-2H-pyran-2-one (1.0 g, 4.06 mmol), isovaletaldehyde (0.35 g, 4.06 mmol), cyclohexyl mercaptan (0.944 g, 8.12 mmol), piperidine (0.5 mL), and o acetic acid (0.5 mL). Melting point 161-162 °C; 1H NMR (400 MHz, DMSO-d6) δ 0.85 (d, 3H), 0.88 (d, 3H), 1.2 (m, 5H), 1.39 (m, 1H), 1.53 (m, 2H), 1.65 (m, 2H), 1.81 (broad m, IH), 2.04 (m, 2H), 4.2 (q, IH), 4.32 (broad q, 4H), 6.53 (s, IH), 6.99 (d, IH), 7.2 (d , IH), 7.25 (dd, IH). IR (KBr) 3099, 2930, 2853, 1649, 1564, 1510, 1397, 1314, 1289, 1260, 1140, 1069, 891,771,608 cm-1. ;Example 130 ;Methyl 2-[[4-[4-hydroxy-2-oxo-3-[(2-phenylethyl)thio]-2H-pyran-6-yl]-phenoxy]-methyl]benzoate ;The title compound is prepared according to method A, using methyl 2-[[(4-acetyl)phenoxy]-methylbenzoate (2.0 g, 7.04 mmol) trimethylsilylfluoromethyl-sulfonate (1.57 g, 7.04 mmol), triethylamine (1.42 g, 14.08 mmol) and diethyl [( 2-phenylethyl)thio]propanedioate (1.04 g, 3.52 mmol). Melting point 161-162 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.78 (t, 2H), 2.97 (t, 2H), 3.81 (s, 3H), 5.5 (s, 2H), 6.69 (s, IH), 7.14 (m, 3H), 7.25 (m, 4H), 7.5 (m, 2H), 7.78 (m, 2H), 7.78 (d, 2H), 7.94 (d, 1H); IR (KBr) 3028, 2949, 2909, 2675, 1715, 1638, 1510, 1402, 1291, 1267, 1181, 1030, 828, 747 cm 1; MS (Cl) m/e 489 (M+H, 51), 384 (3), 353.(1), 149(100), 135(47), 105(33). ;Example 131 ;4-Hydroxy-3-[(2-phenylethyl)thio]-6-[4-(1H-tetrazol-5-ylmethoxy)phenyl]-2H--pyran-2-one ;The title compound was prepared using of the product of Example 143 (0.5 g, 1.32 mmol), trimethyltin azide (0.543 g, 2.64 mmol), toluene (10 mL) and ethanol (10 mL), and this mixture was refluxed for 24 hours. The solvents are steam. 1M HCl was added to the residue, and it was stirred for 2 hours at room temperature. The residue was treated with methanol, the solvents were evaporated, and the resulting solid was washed with ethyl acetate to give the pure compound. Melting point 195-196 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.78 (t, 2H), 2.99 (t, 3H), 5.6 (s, 2H), 6.72 (s, IH), 7.22 (m, 7H), 7.81 (d, 2H); IR (KBr) 3121, 3028, 1657, 1549, 1512, 1410, 1256, 1186, 1059 813, 696 cm 1 ; MS (CI) m/e 423 (M+H, 8), 341 (3), 137 (11), 105 (100). Example 132 4-Hydroxy-6-[3-methyl-4-[(2-pyridinyl)methoxy]phenyl]-3-[(2-phenylethyl)thio]-2H-pyran-2-one Title compound was prepared according to method A, using 4-(2-pyridinylmethoxy)-3-aceto-phenone (2.0 g, 8.29 mmol) trimethylsilylfluoromethyl-sulfonate (1.84 g, 8.29 mmol), triethylamine (1.68 g, 16.58 mmol) and diethyl [( 2-phenylethyl)thio]propanedioate (1.22 g, 4.15 mmol). Melting point 75-77 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.32 (s, 3H), 2.78 (t, 3H), 2.97 (t, 2H), 5.29 (s, 2H), 6.67 (s, 1H), 7.14-7.29 ( m, 4H), 7.38 (m, 1H), 7.56 (m, 2H), 7.67 (m, 2H), 7.86 (t, 2H), 8.61 (d, 1H); IR (KBr) 3063, 2924, 1719, 1603, 1505, 1267, 1138, 1039, 760 cm 1; MS (CI) m/e 446 (M+H, 90), 341 (16), 279 (17), 242 (21), 151 (25), 105 (100); Elemental analysis, calculated for C26H23O4NS: C, 70.09; H, 5.2; N, 3.4; found: C, 70.68; H, 5.28; N, 3.14. ;Example 133 ;3-[2-Cyclopropyl-1-[phenylmethyl)thio]-4-hydroxy-6-phenyl-2H-pyran-2-one (4-/-) ;The title compound was prepared according to method C, using 4-hydroxy-6-phenyl-6-yl]-2H-pyran-2-one (1.5 g, 7.98 mmol), 2-cyclopropylmethylcarboxaldehyde (0.67 g, 7.98 mmol), benzyl mercaptan (1.98 g, 15.96 mmol), piperidine (0.5 mL), and acetic acid (0.5 mL). Melting point 59-61 °C; 1H NMR (400 MHz, DMSO-d6) δ -0.97 (m, 2H), 0.28 (m, 2H), 0.58 (m, IH), 1.61 (m, IH), 2.01 (m, IH), 3.72 (ABXq , 2H), 4.22 (q, IH), 6.67 (s, IH), 7.18 (t, IH), 7.25 (d, 2H, 7.31 (t, 2H), 7.53 (m, 3H), 7.75 (m, 2H ); IR (KBr) 3061, 2919, 2631, 1649, 1564, 1404, 1267, 766, 691 cm-1; MS (CI) m/e 255 (M-SBzl, 19), 201 (5), 147 ( 2); Elemental analysis, calculated for C23H22O3S: C, 72.99; H, 5.86; found: C, 72.31; H, 6.08; Example 134; 4-Hydroxy-3-[1-[(2-methoxyphenyl)thio]- 3-methoxybutyl]-6-phenyl-2H-pyran-2--one (+/-) ; The title compound was prepared according to method C, using 4-hydroxy-6-phenyl-6-yl]-2H-pyran-2 -one (1.5 g, 7.98 mmol), isovaleraldehyde (0.69 g, 7.98 mmol), 3-methoxythiophenol (2.24 g, 15.96 mmol), piperidine (1.0 mL), and acetic acid (1.0 mL). Melting point 7.5-78 ° C; 1H NMR (400 MHz, DMSO-d6) δ 0.86 (d, 3H), 0.89 (d, 3H), 1.53 (m, IH), 1.69 (m, IH), 2.19 (m, IH), 3.64 ( s, 3H), 4.69 (q, IH), 6.64 (s, IH), 6.89 (t, IH), 6.94 (d, IH), 7.17 (t, IH), 7.33 (d, IH), 7.53 (m , 3H), 7.78 (m, 2 H); IR (KBr) 3063, 2955, 2635, 1649, 1594, 1406, 1242, 1026, 768, 750, 691 cm-1; MS (Cl) m/e 257 (M-SPh(OMe), 11), 201 (3), 141 (88); Elemental analysis, calculated for C23H24O4S: C, 69-66; H, 6.10; found: C 69.63; H, 5.92. ;Example 135 ;4-Hydroxy-3-[1-(phenylmethyl)thio]-3-methylbutyl]-6-phenyl-2H-pyran-2-one (+/-) ;The title compound was prepared according to method C, using 4-hydroxy-6-phenyl-6-yl]-2H-pyran-2-one (1.5 g, 7.98 mmol), isovaleraldehyde (0.69 g, 7.98 mmol), benzyl mercaptan (1.98 g, 15.96 mmol), piperidine (1.0 mL ), and acetic acid (1.0 mL). Melting point 153-155 °C; 1H NMR (400 MHz, DMSO-d6) δ 0.64 (d, 3H), 0.81 (d, 3H), 1.25 (m, IH), 1.53 (m, IH), 2.04 (m, IH), 3.69 (ABXq, 2H), 4.22 (q, 1H); IR (KBr) 3086, 2955, 1651, 1566, 1497, 1404, 1311, 1127,912,766(8). ;Example 136 ;Methyl 4-[[4-[4-hydroxy-2-oxo-3-[(2-phenylethyl)thio]-2H-pyran-6-yl]-phenoxy]-methylbenzoate ;The title compound was prepared by method A, using methyl 4-[[(4-acetyl)phenoxy]-methylbenzoate (2.0 g, 7.04 mmol) lithium hexamethyldisilazide (2.36 g, 14.08 mmol) chlorotrimethylsilane (2.38 g, 14.08 mmol) and diethyl [(2-phenylethyl ) thio]propanedioate (1.00 g, 3.05 mmol). Melting point 157-158 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.78 (t, 2H), 2.97 (t, 2H), 3.86 (s, 2H), 5.31 (s, 2H), 6.67 (s, IH), 7.17 (q, 4H), 7.25 (m, 3H), 7.61 (d, 2H), 7.78 (d, 2H), 8.0 (d, 2H); IR (KBr) 3023, 2936, 2581, 1632, 1510, 1404, 1258, 1184, 1098, 1009, 818, 718 cm 1 ; MS (CI) m/e 517 (M+29, 7), 489 (M+H), 55) 384 (19), 149 (40), 105 (100). ;Example 137 ;Methyl 3-[[4-[4-hydroxy-2-oxo-3-[(2-phenylethyl)thio]-2H-pyran-6-yl]-phenoxy]-methyl]benzoate ;The title compound is prepared according to method A, using methyl 3-[[(4-acetyl)phenoxy]-methyl]benzoate (2.0 g, 7.04 mmol) lithium hexamethyldisilazide (2.36 g, 14.08 mmol) chlorotrimethylsilane (2.38 g, 14.08 mmol) and diethyl [ (2-phenylethyl)thio]propanedioate (1.00 g, 3.05 mmol). Melting point 147-149 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.78 (t, 2H), 2.97 (t, 2H), 3.86 (s, 2H), 5.31 (s, 2H), 6.69 (s, IH), 7.2 (m, 7H), 7.58 (t, IH), 7.75 (m, 3H), 7.78 (d, IH), 7.94 (d, IH), 8.08 (s, IH); IR (KBr) 3081, 2950, 1726, 1632, 1609, 1512, 1406, 1345, 1290, 1209, 1098, 1004, 820, 748, 696 cm 1; MS (CI) m/e 489 (M+H), 48) 384 (16), 341 (7), 236 (6), 149 (39), 119 (11), 105 (100). ;Example 138 ;6-[4-(3,4-Dichlorophenylmethoxy]phenyl]-4-hydroxy-3-[(2-phenylethyl)thio]-2H--pyran-2-one ;The title compound was prepared according to method A , using 4-[(3,4-dichlorophenyl)-methoxyacetophenone (2.0 g, 6.80 mmol) lithium hexamethyldisilazide (2.28 g, 13.61 mmol) chlorotrimethylsilane (2.3 g, 13.61 mmol) and diethyl [(2-phenylethyl)thio]propanedioate (1.00 g, 3.40 mmol). Melting point 168-169 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.78 (t, 2H), 2.97 (t, 2H), 5.22 (s, 2H), 6.69 (s , IH), 7.17 (m, 8H), 7.47 (dd, IH), 7.69 (d, IH), 7.78 (d, IH); IR (KBr) 3054, 2602, 1713, 1611, 1512, 1399, 1291, 1179, 1109, 1042, 818, 754 cm-1; MS (CI) m/e 501 (17), 499 (24), 394 (12), 353 (1), 161 (20), 159 (27), 105 (100). ;Example 139 ;Methyl 3-[[(4-hydroxy-2-oxo-6-phenyl-2H-pyran-3-yl]thio]methyl]benzoate ;The title compound was prepared according to method B, using 4-hydroxy-6-phenyl-2H-pyran-2-one (2.0 g, 10.63 mmol), [3-(carbomethoxy)phenyl]methyl-p-toluenethiosulfonate (3.57 g, 10.63 mmol), 1M NaOH (10.63 mL) and ethanol (20 mL).Melting point 17 0-171 °C; 1H NMR (400 MHz, DMSO-d6) δ 3.78 (s, 3H), 4.06 (s, 2H), 6.72 (s, IH), 7.42 (t, IH), 7.53 (m, 4H), 7.78 (m, 3H), 7.83 (s, 1H); IR (KBr) 3108, 2947, 1716, 1644, 1549, 1400, 1302, 1100, 770, 713, 523 cm-1; MS (CI) m/e 369 ((M+H), 7), 337 (8), 235 (6), 189 (4), 149 (11), 85 (100). ;Example 140 ;Methyl 4-[[(4-hydroxy-2-oxo-6-phenyl-2H-pyran-3-yl]thio]methyl]benzoate ;The title compound was prepared according to method B, using 4-hydroxy-6 -phenyl-2H-pyran-2-one (2.0 g, 10.63 mmol), [4-(carbomethoxy)phenyl]methyl-p-toluenethiosulfonate (3.57 g, 10.63 mmol), 1M NaOH (10.63 mL) and ethanol (20 mL ).M.P. 215-216°C; 1H NMR (400 MHz, DMSO-d6) δ 3.81 (s, 3H), 4.06 (s, 2H), 6.69 (IH), 7.39 (d, 2H), 7.67 (m, 3H), 7.81 (m, 2H), 7.86 (d, 2H); IR (KBr) 3110, 3038, 1717, 1644, 1547, 1402, 1279, 1103, 720, 526 cm 1; MS (CI) m/e 369 ((M+H), 22), 335 (100), 207 (18), 189 (37), 151 (55), 119(20), 105 (21), 85 (28). ;Example 141 ; 6-[3,5-Bis(trifluoromethyl]phenyl]-4-hydroxy-3-[(phenylmethyl)thio]-2H-pyran-2-one; The title compound was prepared according to method A, using trimethylsilyl ether of 3\5 '-trifluoromethylacetophenone (2.16 g, 71 mmol) [prepared using 3,5-trifluoromethylacetophenone (15 g, 58.55 mmol), trimethylsilyl-trimethylsulfonate (13.01 g, 58.55 mmol) and triethylamine (11.84 g, 117.10 mmol)] and diethyl [(phenylmethyl)thio]propanedioate (1.00 g, 3.55 mmol), distilled]. Melting point 80-82 °C; 1H NMR (400 MHz, DMSO-d6) δ 4.0 (s, 2H), 6.61 (s, IH), 7.22 (m, 2H), 7.28 (m, 3H), 7.97 (s, IH), 8.25 (s, 2H); IR (KBr) 3090, 1726, 1682, 1638, 1549, 1530, 1385, 1281, 1182, 1138, 902, 700 cm-1; MS (Cl) m/e 475 (M+29, 3), 447 (M+H, 21), 213 (1), 149 (2), 91 (100). ;Example 142 ;3-[1-(Cyclohexylthio)-3-methylbutyl]-4-hydroxy-6-phenyl-2H-pyran-2-one (+/-) ;The title compound was prepared according to method C, using 4- hydroxy-6-phenyl-6-yl]-2H-pyran--2-one (1.5 g, 7.98 mmol), isovaleraldehyde (0.76 g, 8.78 mmol), cyclohexyl mercaptan (2.04 g, 17.56 mmol), piperidine (1.0 mL) , acetic acid (1.0 mL), and ethanol (20 mL). Melting point 210-212 °C; 1H NMR (400 MHz, DMSO-d6) δ 0.89 (t, 6H), 1.36 (m, 6H), 1.44 (m, 1H), 1.56 (m, 2H), 1.69 (m, 2H), 1.81 (m, IH), 2.08 (m, 2H), 2.61 (broad s, IH), 4.22 (m, IH), 6.67 (s, IH), 7.53 (m, 3H), 7.78 (m, 2H); IR (KBr) 3106, 2928, 2851, 1659, 1568, 1404, 1125, 766, 569; MS (CI) m/e 259 (50), 257 (49), 201 (46), 189 (16), 147(8), 105(28), 83(100). ;Example 144 ;4-Hydroxy-3-[(2-isopropylphenyl)thio]-6-phenyl-2H-pyran-2-one ;The title compound was prepared according to method A, using 1-phenyl-1-(trimethylsilyloxy)ethylene (1.24 g, 6.45 mmol) and diethyl [(2-isopropylphenyl)thio]propanedioate (1.0 g, 3.23 mmol). Melting point 157-159 °C; 1H NMR (400 MHz, DMSO-d6) δ 1.25 (d, 6H), 3.42 (m, IH), 6.89 (s, IH), 6.92 (dd, IH), 7.06 (t, IH), 7.13 (t, 1H), 7.28 (d, 1H), 7.56 (m, 3H), 7.85 (m, 2H); IR (KBr) 3117, 2962, 1661, 1551, 1406, 1365, 1101, 760 cm-1; MS (CI) m/e 380 (100), 275 (60), 205 (8), 105 (94); Elemental analysis, calculated for C 20 H 18 O 3 S: C, 70.98; H, 5.36; found: C, 70.82; H, 5.24. ;Example 143 ;[4-[4-Hydroxy-2-oxo-3-[(2-phenylethyl)thio]-2H-pyran-6-yl]phenoxy]-acetonitrile ;The title compound was prepared according to method A, using the appropriate acetophenone (3.0 g, 17.12 mmol), trimethylsilylfluoromethyl-sulfonate (3.8 g, 17.12 mmol), triethylamine (3.46 g, 34.24 mmol) and diethyl [(2-phenylethyl)thio]propanedioate (2.53 g, 8.56 mmol). Melting point 157-159 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.92 (t, 2H), 3.11 (t, 2H), 4.86 (s, 2H), 6.56 (s, 2H), 7.08 (d, 2H), 7.19 (t, 3H), 7.3 (m, 3H), 7.86 (d, 2H); IR (KBr) 2993, 2577, 1634, 1510, 1404, 1342, 1302, 1226, 1188, 1051, 833, 717, 505 cm-1; MS (CI) m/e 380 (100), 275 (60), 205 (8), 105 (94). ;Example 144 ;4-Hydroxy-3-[(2-isopropylphenyl)thio]-6-phenyl-2H-pyran-2-one ;The title compound was prepared according to method A, using 1-phenyl-1-(trimethylsilyloxy)ethylene (1.24 g, 6.45 mmol) and diethyl [(2-isopropylphenyl)thio]propanedioate (1.0 g, 3.23 mmol). 1H NMR (400 MHz, DMSO-d6) δ 1.25 (d, 6H), 3.42 (m, IH), 6.89 (s, IH), 6.92 (dd, IH), 7.06 (t, IH), 7.13 (t, 1H), 7.28 (d, 1H), 7.56 (m, 3H), 7.85 (m, 2H); IR (KBr) 3117, 2962, 1661, 1551, 1406, 1365, 1101, 760 cm-1; MS (CI) m/e 339 (100), 305 (4), 219 (25), 189 (11), 147 (9), 105 (9); Elemental analysis, calculated for C20H18O3S: C, 70.98; H, 5.36; found: C, 70.82; H, 5.24. ;Example 145 ;3-[(Cyclopropylmethyl)thio]-4-hydroxy-6-phenyl-2H-pyran-2-one ;The title compound (0.053 g, melting point 136-137 °C) was prepared according to method B, using 4 -hydroxy-6-phenyl-2H-pyran-2-one (0.250 mg, 1.33 mmol), cyclopropimethyl-p-toluene-thiosulfonate (0.585 g, 2.261 mmol), triethylamine (0.158 mL, 1.46 mmol), sodium bicarbonate ( 0.110 g, 1.33 mmol), and ethanol (10.0 mL). 1H NMR (250 MHz, CDCl3) δ 7.994-7.726 (m, 2H), 7.683-7.406 (m, 3H), 6.665 (s, IH), 2.724-2.694 (d, 2H, J=7.3 Hz), 1.063- 0.903 (m, 1H), 0.608-0.533 (m, 2H), 0.270-0.208 (m, 2H). ;Example 146 ;6-(3-Chlorophenyl)-4-hydroxy-3-[(4-phenylbutyl)thio]-2H-pyran-2-one ;The title compound (0.024 g, melting point 123-124 °C) was prepared according to method B, using 6-(3-chlorophenyl)-4-hydroxy-2H-pyran-2-one (0.250 mg, 1.13 mmol), 4-phenylbutyl-p-toluene-thiosulfonate (0.45 g, 1.93 mmol), triethylamine (0.115 g, 1.13 mmol), sodium bicarbonate (0.094 g, 1.13 mmol), and ethanol (5.0 mL). 1H NMR (400 MHz, CDCl3) δ 7.848-7.839 (m, 1H), 7.729 (m, 1H), 7.479-7.392 (m, 2H), 7.276-7.239 (m, 2H), 7.174-7.137 (m, 3H) ), 6.631 (s, 1H), 2.831-2.794 (t, 2H), 2.633-2.596 (t, 2H), 1.747-1.689 (m, 2H), 1.649-1.591 (m, 2H). ;Example 147 ;4-Hydroxy-3-[(2-oxo-2-phenylethyl)thio]-6-phenyl-2H-pyran-2-one ;Solutions of 4-hydroxy-3-mercapto-6-phenyl-2- of pyrone (0.175 g, 0.840 mmol, prepared as described in R.F. Harris, J.E. Dunbar, U.S. Patent 3,181,046) in CH2Cl2 (3 mL) was added under N2 first triethylamine (0.12 mL, 0.84 mmol) and then bromoacetophenone (0.167 g , 0.840 mmol). The mixture was left to stir for 30 minutes at room temperature, and then the solvent was removed in vacuo. The residue was diluted with diethyl ether and extracted with saturated Na2CO3 solution (3 x 50 mL). Aqueous layers are combined, acidified conc. HCl, and were extracted with CH2Cl2 (3 x 100 mL). The organic layers were combined, dried over Na2SO4, and the solvent was removed in vacuo to give the title compound (0.066 g, m.p. 164-166 °C) which was dried in vacuo. 1H NMR (300 MHz, CDCl3) δ 9.900 (broad s, IH), 7.970 )d, 2H, J=7.1 Hz), 7.810 (d, 2H, J=8 Hz), 7.615 (t, IH, J=4 Hz), 7.505-7.443 (m, 5H), 6.619 (s, 1H), 4.334 (s, 2H). ; Example 148 ; 4-Hydroxy-3-[(2-phenylethan-2-ol)thio]-6-phenyl-2H-pyran-2-one ; Solution of 4-hydroxy-3-[(2-oxo-2- phenylethyl)thio]-6-phenyl-2-pyrone (0.021 g, 0.060 mmol) in THF (10 mL) was cooled to 0 °C with stirring, and a 1.0 M solution of BH3·DMS (0.05 mL) was added by injection in an N2 atmosphere , 0.05 mmol) in THF. The mixture was left with stirring for 1 hour, and the reaction was "quenched" by the addition of a 1:1 mixture of 4 M HCl : MeOH. The mixture was then extracted with diethyl ether. The layers were combined, dried over Na2SO4, and the solvent was evaporated in vacuo to give the title compound (0.015 g) as an oil.*1H NMR (200 MHz, CDCl3) δ 7.873-7.777 (m, 4H), 7.516- 7-153 (m, 6H), 6.667 (s, IH), 4.820-4.755 (dd, IH, J=9.8 Hz, 3.2 Hz), 3.212-3.127 (dd, IH, J=13.8 Hz, 3.2 Hz), 2.920 (dd, IH, J=9.8 Hz, 13.8 Hz).

Example 149

4-Hydroxy-5-methyl-6-phenyl-3-[phenylthio]-2H-pyran-2-one

A solution of propiophenone (1.50 mL, 11.3 mmol) in CH2Cl2 (4 mL) was cooled to 0 °C (N2 atmosphere), and triethylamine (3.14 mL, 22.6 mmol) was added, followed by trimethylsilyl triphthalate (2.60 mL, 13.5 mmol). . The solution was warmed to room temperature, left with stirring for 15 minutes, and the reaction was "quenched" in diethyl ether (50 mL) and then in saturated NaHCO3 solution (20 mL). The layers were separated, and the organic layer was washed with a 1:1 mixture of sodium chloride solution/saturated NaHCO3 solution (20 mL). The ethereal solution was dried over Na2SO4, and the solvent was removed in vacuo. The resulting silylenol ether was transferred to a flask containing diethyl 2-(thiophenyl)propane-1,3-dioate (1.00 g, 3.76 mmol) and the mixture was heated for 16 h at 140 °C and allowed to cool to room temperature. temperature, after which it was diluted with diethyl ether and extracted with saturated Na2CO3 (3 x 20 mL). The aqueous layers were combined, washed with diethyl ether (3 x 75 mL, i. and carefully acidified with conc. HCl. The mixture was then extracted with CH2Cl2 (3 x 200 mL), the organic layers were combined, dried over Na2SO4, and the solvent removed in vacuo to give the title compound (0.350 g, mp 166-167 °C).1H NMR (400 MHz, DMSO-d6) δ 6.309-6.285 (m, 2H), 6.227-6.211 (m, 3H) , 5.983 (t, 2H, J=8 Hz), 5.862 (d, 2H, J=8 Hz), 0.705 (s, 3H).

Example 150

[4-[4-Hydroxy-2-oxo-3-(phenylthio)-2H-pyran-6-yl]phenoxy]acetic acid

To a solution of methyl-[4-(1-oxoethyl)phenoxy]-acetate (2.50 g, 10.86 mmol) in CH2Cl2 (25.0 mL) was added triethylamine (3.03 mL, 21.7 mmol) at 0 °C (N2 atmosphere), followed by trimethylsilyl --triphthalate (2.52 mL, 13.0 mmol). The solution was warmed to room temperature, and was left with stirring for 15 minutes, and then "quenched" in diethyl ether (50 mL) and in saturated NaHCO3 solution (20 mL). The layers were separated, and the organic layer was washed with a 1:1 mixture of sodium chloride solution/saturated NaHCO3 solution (20 mL). The ethereal solution was dried over Na2SO4, and the solvent was removed in vacuo. The resulting silylenol ether was transferred to a flask containing diethyl 2-(thiophenyl)propane-1,3-dioate (0.97 g, 3.6 mmol) and the mixture was heated at 140°C for 16 hours and allowed to cool to room temperature. temperature, after which it was subjected to chromatography (SiO2 230-400 mesh, 100% CH2C12 to 2% MeOH/CH2Cl2), whereby an impure solid was obtained, which was diluted with diethyl ether (20 mL) and extracted with saturated Na2CO3 solution 83 x 20 mL ). The combined aqueous layers were washed with diethyl ether (3 x 100 mL), and the conc. HCl to pH 0. The mixture was then extracted with ethyl acetate (3 x 100 mL), the organic layers were combined, dried over Na2SO4, and the solvent was removed in vacuo to give the title compound (0.695 g, mp 186-188 ° C). 1H NMR (300 MHz, DMSO-d6) δ 13.175 (broad s, 1H), 12.425 'broad s, 1H), 7.809 (d, 2H, J=9 Hz), 7.298-7.247 (m, 2H), 7.149- 7.004 (m, 5H), 6.785 (s, 1H), 4.804 (s, 2H).

Example 151

[4-[4-Hydroxy-5-methyl-2-oxo-3-(phenylthio)-2H-pyran-6-yl]phenoxy]acetic acid

The title compound (0.691 g, mp 194-197 °C) was prepared in a similar manner as shown for the preparation of [4-[4-hydroxy-2-oxo-3-(phenylthio)-2H-pyran-6-yl] phenoxy]acetic acid, using methyl-[4-(l-oxoethyl)phenoxy]-acetate (2.00 g, 8.81 mmol), triethylamine (3.68 mL, 26.5 mmol) trimethylsilyl triphthalate (2.38 mL, 12.3 mmol), dichloromethane (20.0 mL), diethyl 2-(thiophenyl)propane-1,3-dioate (1.34 g, 5.00 mmol). 1H NMR (400 MHz, DMSO-d6) δ 7.585 (d, 2H, J=9 Hz), 7.325-7.286 (m, 2H), 7.178 (d, 3H, J=7.5 Hz), 7.063 (d, 2H, J=9 Hz), 4.784 (s, 2H), 2.042 (s, 3H).

Example 152

Hydroxy-3-phenoxy-6-phenyl-2H-pyran-2-one

Diethyl 2-phenoxypropanedioate (8.11 g (0.032 mmol) and 1-phenyl-1-(trimethylsilyloxo)ethylene 12.35 g (0.064 mmol) were added to the high pressure reactor. The vessel was pressurized to 600 psi with N2. The mixture was heated 8 hours at 100 °C and an additional 63.5 hours at 147-154 °C. The vessel was cooled to room temperature, washed with ethyl acetate. The crude product was subjected to "flash" chromatography (hexane/ethyl acetate 1/1), and thus the partially purified material was subjected again to "flash" chromatography on silica gel using hexane/ethyl acetate 95/5 - 40/60 as eluent. The resulting solid was recrystallized from diethyl ether and ethyl acetate to give 1.54 (18% ) of the title compound (mp 215-219 °C). 1H NMR (400 MHz, DMSO-d6) δ 6.90 (s, IH), 6.95 (dd, 2H), 7.02 (t, IH), 7.28-7.33 (m, 2H), 7.52-7.56 (m, 3H), 7.80-7.856 (m, 2H), 12.0 (broad s, 1H).

Example 153

4-Hydroxy-3-[(phenylmethyl)thio]-6-(3-pyridinyl)-2H-pyran-2-one

The title compound was prepared by condensation of trimethylsilylenol ether from 3-acetylpyridine and diethyl [(phenylethyl)thio]propanedioate following the procedure described in general method A. Melting point 183-184 °C. 1H NMR (DMSO-d6) δ 4.02 (s, 2H), 6.83 (s, IH), 7.20 (m, IH), 7.26 (d, 4H), 7.55 (m, IH), 8.16 (m, IH), 8.69 (m, IH), 8.98 (d, IH).

Example 154

6-(2,6-Dieneethyl-4-pyridinyl)-4-hydroxy-3-[(phenylmethyl)thio]-2H-pyran-2-one

The title compound was prepared by condensation of trimethylsilylenol ether from 4-acetyl-2,6-dimethyl-pyridine and diethyl [(phenylethyl)thio]propanedioate following the procedure described in general method A. Melting point 88-90 °C. 1H NMR (DMSO-d6) δ 2.55 (s, 6H), 4.02 (s, 2H), 6.85 (s, 1H), 7.16-7.28 (m, 5H), 7.40 (s, 2H).

Example 155

4-Hydroxy-3-[(phenylmethyl)thio]-6-(3-thienyl)-2H-pyran-2-one

The title compound was prepared by condensation of trimethylsilylenol ether from 3-acetylthiophene and diethyl [(phenylethyl)thio]propanedioate following the procedure described in general method A. Melting point 150-151 °C. 1H NMR (DMSO-d6) δ 3.98 (s, 2H), 6.58 (s, 1H), 7.24 (m, 5H), 7.48 (m, 1H), 7.72 (m, 1H), 8.13 (d, 1H).

Example 156

3-[[(2,6-Dimethylphenylmethyl]thio]-4-hydroxy-6-phenyl-2H-pyran-2-one

The title compound was prepared according to method B, using 4-hydroxy-6-phenyl-2H-pyran-2-one (1.0 g, 5.31 mmol), ethanol (15 mL), 1M NaOH (5.31 mL) and (2,6-dimethylphenyl )methyl-(Moluentiosulfonate) (1.62 g, 5.31 mmol), i. Melting point 231-233 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.34 (s, 6H), 4.01 (s, 2H), 6.81 (m, 2H), 7.03 (m, 3H), 7.53 (m, 3H), 7.82 (m, 2H).

Example 157

4-Hydroxy-6-phenyl-3-[[(3-phenoxyphenyl)methyl]thio]-2H-pyran-2-one

The title compound was prepared according to method B, using 4-hydroxy-6-phenyl-2H-pyran-2-one (1.0 g, 5.31 mmol), ethanol (15 mL), 1M NaOH (5.31 mL), and (3-phenoxy)methyl -p-toluenethiosulfonate (1.96 g, 5.31 mmol). Melting point 131-133 °C; 1H NMR (400 MHz, DMSO-d6) δ 3.97 (s, 2H), 6.73 (s, 1H), 6.87 (m, 4H), 7.03 (m, 2H), 7.27 (m, 3H), 7.53 (m, 3H9, 7.78 (m, 2H).

Example 158

3-[1-[(Cyclohexylmethyl)thio]-3-methylbutyl]-4-hydroxy-6-phenyl-2H-pyran-2-one (+/-)

The title compound was prepared according to method C, using 4-hydroxy-6-phenyl-2H-pyran-2-one (1.0 g, 5.31 mmol), ethanol (10 mL), isovaletaldehyde (0.462 g, 5.84 mmol), cyclohexylmethylthiol (1.79 g, 13.8 mmol), piperidine (0.5 mL), and acetic acid (0.5 mL). Melting point 146-148 °C; 1H NMR (400 MHz, DMSO-d6) δ 0.58 (d, 6H), 1.11 (m, 5H), 1.57 (m, 8H), 2.07 (m, IH), 2.28 (dd, IH), 2.38 (dd, IH), 4.17 (dd, IH), 6.69 (s, IH), 7.54 (m, 3H), 7.75 (m, 2H), 11.71 (broad s, IH).

Example 159

3-[1-[(Cyclohexylmethyl)thio]phenylniethyl]-4-hydroxy-6-phenyl-2H-pyran-2-one (+/-)

The title compound was prepared according to method C, using 4-hydroxy-6-phenyl-2H-pyran-2-one (1.0 g, 5.31 mmol), ethanol (10 mL), benzaldehyde (0.593 g, 5.84 mmol), cyclohexylmethylthiol (1.79 g, 13.8 mmol), piperidine (0.5 mL), and acetic acid (0.5 mL). Melting point 138-141 °C; 1H NMR (400 MHz, DMSO-d6) δ 0.89 (m, 2H), 1.21 (m, 3H), 1.42 (m, 1H), 1.61 (m, 3H), 1.75 (m, 2H), 2.39 (m, 2H), 5.30 (s, 1H), 6.71 (s, 1H), 7.26 (t, 1H), 7.28 (t, 2H), 7.53 (m, 5H), 7.74 (m, 2H).

Example 160

4-Hydroxy-6-[4-(2-hydroxyethoxy)phenyl]-3-[(2-phenylethyl)thio]-2H-pyran-2--one

Lithium borohydride (0.5 mL, 1.00 mmol). The reaction was stirred overnight. The reaction was then "quenched" with additional 1M hydrochloric acid (2.0 mL). The organic layer was separated and washed with saturated sodium chloride solution and dried over magnesium sulfate. After evaporation of the solvent in vacuo, the crude product was purified by column chromatography (silica gel 230-400 mesh) using 50% ethyl acetate/hexane to 100% ethyl acetate as eluent. Melting point 123-125 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.77 (t, 2H), 2.97 (t, 2H), 3.37 (m, 2H), 4.07 (t, 2H), 4.92 (broad s, IH), 6.68 (s , 1H), 7.09 (d, 2H), 7.19 (m, 5H), 7.75 (d, 2H).

Example 161

Ethyl 3-[4-hydroxy-2-oxo-3-[(2-phenylethyl)thio]-2H-pyran-6-yl]phenoxy]acetate

The title compound was prepared according to method A, using ethyl (3-acetylphenoxy)acetate (2.00 g, 9.00 mmol), trimethylsilyl triphthalate (4.18 mL, 21.62 mmol), triethylamine (5.01 mL, 36.00 mmol) methylene chloride (23 mL) and diethyl [(2-phenylethyl)thio]propanedioate (1.0 g, 3.37 mmol). Melting point 116-119 °C; 1H NMR (400 MHz, DMSO-d6) δ 1.22 (t, 3H), 2.77 (t, 2H), 3.05 (t, 2H), 4.89 (d, 2H), 6.8 (s, IH), 7.20 (m, 7H), 7.44 (m, 2H).

Example 162

4-Hydroxy-6-[4-[(5-methyl-3-phenyl-4-isoxazolyl)methoxy]phenyl]-3-[(2-phenyl-ethyl)thio]-2H-pyran-2-one

The title compound was prepared according to method A, using 4!-[(5-methyl-3-phenyl-4-isoxazoyl)-methoxy]acetophenone (2.00 g, 6.51 mmol), trimethylsilyl triphthalate (1.51 mL, 7.81 mmol), triethylamine (1.81 mL, 13.02 mmol) methylene chloride (16 mL) and diethyl [(2-phenylethyl)thio]-propanedioate (1.0 g, 3.37 mmol). Melting point 126-128 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.54 (s, 3H), 2.77 (t, 2H), 2.98 (t, 2H), 5.08 (s, 2H), 6.69 (s, IH), 7.21 (m, 7H), 7.49 (m, 3H), 7.71 (m, 2H), 7.77 (d, 2H).

Example 163

6-(3,5-Dimethylphenyl)-4-hydroxy-3-(phenylthio)-2H-pyran-2-one

The title compound was prepared according to method A, using 3',5'-dimethylacetophenone (1.43 g, 9.70 mmol), trimethylsilyl triphthalate (2.24 mL, 11.64 mmol), triethylamine (2.70 mL, 19.40 mmol) methylene chloride (24 mL) and diethyl (phenylthio)propanedioate (1.0 g, 7.46 mmol). Melting point 210-211 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.35 (s, 6H), 6.83 (s, IH), 7.12 (m, 3H), 7.21 (s, IH), 7.27 (t, 2H), 7.46 (s, 2H).

Example 164

3-[1-[Cyclopentylthio]-2-cyclopropylethyl]-4-hydroxy-6-phenyl-2H-pyran-2-one-(+/-)

The title compound was prepared according to method C, using 4-hydroxy-6-phenyl-2H-pyran-2-one (1.0 g, 5.31 mmol), ethanol (10 mL), cyclopropylmethylcarboxaldehyde (0.892 g, 10.62 mmol), cyclopentylthiol (1.43 mL, 13.8 mmol), piperidine (0.5 mL), and acetic acid (0.5 mL). Melting point 75-80 °C; 1H NMR (400 MHz, DMSO-d6) δ 0.04 (m, 2H), 0.07 (m, 2H), 0.66 (m, 1H), 1.53 (m, 7H), 1.94 (m, 3H), 3.19 (m, IH), 4.21 (dd, IH), 6.71 (s, IH), 7.54 (m, 3H), 7.76 (m, 2H).

Example 165

N-[3-[4-Hydroxy-2-oxo-3-[(2-phenylethyl)thio]-2H-pyran-6-yl]phenyl]-4-methyl-benzenesulfonamide

The title compound was prepared according to method A, using 3'-(p-toluenesulfonamide)-acetophenone (1.38 g, 5.06 mmol), trimethylsilyl triphthalate (2.34 mL, 12.41 mmol), triethylamine (2.82 mL, 20.24 mmol) methylene chloride ( 18 mL) and diethyl [(2-phenylethyl)thio]-propanedioate (1.0 g, 3.37 mmol). Melting point 133-135 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.32 (s, 3H), 2.77 (t, 2H), 3.09 (t, 2H), 6.68 (s, 1H), 7.19 (m, 6H), 7.40 (m, 4H), 7.53 (s, IH), 7.67 (d, 2H), 10.50 (s, IH), 12.03 (broad s, IH).

Example 166

3-[Cyclopentyl(cyclopentylthio)methyl]-4-hydroxy-6-phenyl-2H-pyran-2-one (+/-)

The title compound was prepared according to method C, using 4-hydroxy-6-phenyl-2H-pyran-2-one (1.0 g, 5.31 mmol), ethanol (10 mL), cyclopentanecarboxaldehyde (0.780 g, 7.96 mmol), cyclopentylthiol (1.43 mL, 13.8 mmol), piperidine (0.5 mL), and acetic acid (0.5 mL). Melting point 139-142 °C; 1H NMR (400 MHz, DMSO-d6) δ 1.03 (m, IH), 1.64 (m, 15H), 2.64 (m, IH), 3.00 (m, IH), 3.84 (d, IH), 6.69 (s, IH), 7.52 (m, 3H), 7.76 (m, 2H), 11.55 (broad s, IH).

Example 167

6-(1,1-Biphen-3-yl)-4-hydroxy-3-[(2-isopropylphenyl)thio]-2H-pyran-2-one-(+/-)

The title compound was prepared according to method A, using 3'-phenylacetophenone (0.946 g, 4.83 mmol), trimethylsilyl triphthalate (1.12 mL, 5.79 mmol), triethylamine (1.34 mL, 9.66 mmol), methylene chloride (17 mL), and diethyl [ (2-isopropylphenyl)thio]propanedioate (1.0 g, 3.22 mmol). Melting point 193-195 °C; 1H NMR (400 MHz, DMSO-d6) δ 1.25 (d, 6H), 3.43 (m, IH), 6.95 (d, IH), 7.02 (s, IH), 7.06 (t, IH), 7.13 (t, IH), 7.28 (d, IH), 7.42 (t, IH), 7.51 (t, 2H), 7.66 (t, IH), 7.75 (d, 2H), 7.85 (t, 2H), 8.07 (s, IH ).

Example 168

4-Hydroxy-6-phenyl-3-[(2-propylphenyl)thio]-2H-pyran-2-one

The title compound was prepared according to method A, using 1-phenyl-1-(trimethylsilyloxy)ethylene (0.990 g, 4.83 mmol) and diethyl [(2-propylphenyl)thio]propanedioate (1.0 g, 3.22 mmol). Melting point 158-160 °C; 1H NMR (400 MHz, DMSO-d6) δ 0.972 (t, 3H), 1.64 (m, 2H), 2.71 (t, 2H), 6.87 (s, IH), 6.92 (m, IH), 7.06 (m, 2H), 7.16 (m, 1H), 7.55 (m, 3H), 7.85 (m, 2H).

Example 169

6-(3,5-Dimethylphenyl)-4-hydroxy-3-[(2-isopropylphenyl)thio]-2H-pyran-2-one

The title compound was prepared according to method A, using 3',5'-dimethylacetophenone (0.714 g, 4.83 mmol), trimethylsilyl triphthalate (1.12 mL, 5.79 mmol), triethylamine (1.34 mL, 9.66 mmol) methylene chloride (17 mL) and diethyl [(2-isopropylphenyl)thio]propanedioate (1.00 g, 3.22 mmol). Melting point 154-155 °C; 1H NMR (400 MHz, DMSO-d6) δ 1.24 (d, 6H), 2.35 (s, 6H), 3.40 (m, IH), 6.90 (d, IH), 7.05 (t, IH), 7.11 (dt, 2H), 7.20 (s, 1H), 7.27 (d, 1H), 7.45 (s, 2H).

Example 170

4-Hydroxy-6-(4-hydroxyphenyl)-3-[(2-isopropylphenyl)thio]-2H-pyran-2-one

The title compound was prepared according to method A, using 4'-hydroxyacetophenone (0.657 g, 4.83 mmol), trimethylsilyl triphthalate (2.05 mL, 10.62 mmol), triethylamine (2.69 mL, 19.32 mmol), methylene chloride (20 mL) and diethyl [ (2-isopropyl phenyl)thio]propanedioate (1.00 g, 3.22 mmol). Melting point 250 °C with decomposition; 1H NMR (400 MHz, DMSO-d6) δ 1.24 (d, 6H), 3.40 (m, IH), 6.70 (s, IH), 6.90 (t, 3H), 7.05 (t, IH), 7.10 (t, 1H), 7.26 (d, 1H), 7.70 (d, 2H).

Example 171

3-[[2-(Cyclopropylmethyl)phenyl]thio]-4-hydroxy-6-phenyl-2H-pyran-2-one

The title compound was prepared according to method A, using l-phenyl-l-(trimethylsilyloxy)ethylene (0.990 mL, 4.83 mmol) and diethyl [[2-(cyclopropylmethyl)phenyl]thio]propanedioate (1.00 g, 3.10 mmol). Melting point 165-167 °C; 1H NMR (400 MHz, DMSO-d6) δ 0.25 (dd, 2H), 0.52 (dd, 2H), 1.21 (m, IH), 2.50 (d, 2H), 6.87 (s, IH), 6.92 (m, 1H), 7.05 (m, 2H), 7.32 (m, 1H), 7.56 (m, 3H), 7.86 (m, 2H).

Example 172

4-Hydroxy-3-[(2-isopropylphenyl)thio]-6-[4-(pyridin-3-ylmethoxy)phenyl]-2H--pyran-2-one

The title compound was prepared according to method A, using 4'-(pyridin-3-ylmethoxy)acetophenone (1.09 g, 4.83 mmol), trimethylsilyl triphthalate (1.12 mL, 5.97 mmol), triethylamine (1.34 mL, 9.66 mmol) methylene chloride (17 mL) and diethyl [(2-isopropylphenyl)thio]propanedioate (1.00 g, 3.22 mmol). Melting point 225 °C; 1H NMR (400 MHz, DMSO-d6) δ 1.23 (d, 6H), 3.41 (m, IH), 5.26 (s, 2H), 6.78 (s, IH), 6.90 (d, IH), 7.08 (dt, 2H), 7.21 (d, 2H), 7.29 (d, IH), 7.45 (dd, IH), 7.82 (d, 2H), 7.91 (d, IH), 8.56 (d, IH), 8.71 (s, IH ).

Example 173

Ethyl 4-[4-Hydroxy-5-[(2-isopropylphenyl)thio]-6-oxo-6H-pyran-2-yl]phenoxy-acetate

The title compound was prepared according to method A, using (4-acetylphenoxy)acetate (2.14 g, 9.67 mmol), trimethylsilyl triphthalate (4.48 mL, 23.20 mmol), triethylamine (13.93 mL, 38.6 mmol), methylene chloride (20 mL) and diethyl [(2-isopropylphenyl)thio]propanedioate (2.00 g, 6.45 mmol). Melting point 194-196 °C; 1H NMR (400 MHz, DMSO-d6) δ 1.57 (m, 9H), 3.41 (m, IH), 4.81 (q, 2H), 4.89 (s, 2H), 6.75 (s, IH), 6.90 (d, 1H), 7.05 (m, 4H), 7.26 (d, 1H), 7.79 (d, 2H).

Example 174

4-[4-Hydroxy-5-[(2-isopropylphenyl)thio]-6-oxo-6H-pyran-2-yl]phenoxy-acetic acid

To a solution of ethyl 4-[4-Hydroxy-5-[(2-isopropylphenyl)thio]-6-oxo-6H-pyran-2-yl]phenoxyacetate (0.319 g, 0.75 mmol) in tetrahydrofuran (10 mL) was added 1M sodium hydroxide (1.80 mL, 1.81 mmol). The reaction was stirred for 1.5 hours, after which water (10 mL) was added, followed by acidification of conc. with hydrochloric acid to pH 2. The aqueous layer was then extracted 2 x with ethyl acetate (100 mL), the combined organic extracts were washed with saturated sodium chloride solution and dried over anhydrous magnesium sulfate. After evaporation of the solvent in vacuo, the crude product was purified by column chromatography (silica gel 230-400 mesh) using 94/5/1 methylene chloride/methanol/acetic acid as eluent. Melting point 217 °C with decomposition; 1H NMR (400 MHz, DMSO-d6) δ 1.25 (d, 6H), 3.42 (m, IH), 4.79 (s, 2H), 6.75 (s, IH), 6.90 (d, IH), 7.06 (m, 4H), 7.26 (d, 1H), 7.79 (d, 2H).

Example 175

4-Hydroxy-3-[(2-isopropylphenyl)thio]-6-(4-methoxyphenyl)-2H-pyran-2-one

The title compound was prepared according to method A, using 4!-methoxyacetophenone (2.26 g, 15.1 mmol), trimethylsilyl triphthalate (3.5 mL, 18.1 mmol), triethylamine (4.26 mL, 30.6 mmol), methylene chloride (30 mL) and diethyl [ (2-isopropylphenyl)thio]propanedioate (3.11 g, 10.0 mmol). Melting point 221-223 °C; 1H NMR (400 MHz, DMSO-d6) δ 1.25 (d, 6H), 3.40 (m, IH), 3.85 (s, 3H), 6.78 (s, IH), 6.92 (m, IH), 7.10 (m, 4H 7.27 (m, IH), 7.81 (d, 2H), 12.38 (broad s, IH).

Example 176

4-Hydroxy-3-[(2-isopropylphenyl)thio]-6-(4-methylphenyl)-2H-pyran-2-one

The title compound was prepared according to method A, using 4!-methylacetophenone (2.02 mL, 15.1 mmol), trimethylsilyl triphthalate (3.5 mL, 18.1 mmol), triethylamine (4.26 mL, 30.6 mmol), methylene chloride (30 mL), and diethyl [ (2-isopropylphenyl)thio]propanedioate (3.11 g, 10.0 mmol). Melting point 191-193 °C; 1H NMR (400 MHz, DMSO-d6) δ 1.25 (d, 6H), 2.39 (s, 3H), 3.41 (m, IH), 6.84 (s, IH), 6.92 (m, IH), 7.10 (m, 2H), 7.27 (m, 1H), 7.37 (m, 2H), 7.75 (d, 2H).

Example 177

6-(3,4-Dichlorophenyl)-4-hydroxy-3-[(2-isopropylphenyl)thio]-2H-pyran-2-one

The title compound was prepared according to method A, using 3',4'-dichloroacetophenone (2.46 g, 12.8 mmol), trimethylsilyl triphthalate (3.0 mL, 15.4 mmol), triethylamine (3.6 mL, 26.0 mmol) methylene chloride (30 mL) and diethyl [(2-isopropylphenyl)thio]propanedioate (4.0 g, 12.8 mmol). Melting point 204-207 °C; 1H NMR (400 MHz, CDCl3) δ 1.33 (d, 6H), 3.55 (m, IH), 6.71 (s, IH), 7.00 (m, IH), 7.08 (m, IH), 7.20 (m, IH) , 7.30 (m, IH), 7.56 (d, IH), 7.69 (m, IH), 7.74 (broad s, IH), 7.98 (s, IH).

Example 178

6-(4-Chlorophenyl)-4-hydroxy-3-[(2-isopropylphenyl)thio]-2H-pyran-2-one

The title compound was prepared according to method A, using 4'-chloroacetophenone (2.33 g, 15.1 mmol), trimethylsilyl triphthalate (3.5 mL, 18.1 mmol), triethylamine (4.26 mL, 30.6 mmol), methylene chloride (30 mL), and diethyl [ (2-isopropylphenyl)thio]propanedioate (3.11 g, 10.0 mmol). Melting point 148-151 °C; 1H NMR (400 MHz, DMSO-d6) δ 1.25 (d, 6H), 3.41 (m, IH), 6.86 (s, IH), 6.92 (m, IH), 7.08 (m, 2H), 7.27 (m, 1H), 7.62 (m, 2H), 7.86 (m, 2H).

Example 179

Ethyl 4-[4-Hydroxy-5-[(2-isopropylphenyl)thio]-6-oxo-6H-pyran-2-yl]benzoate

The title compound was prepared according to method A, using 4-acetylbenzoate (2.93 g, 15.1 mmol), trimethylsilyl triphthalate (3.5 mL, 18.1 mmol), triethylamine (4.26 mL, 30.6 mmol), methylene chloride (30 mL), and diethyl [( 2-isopropylphenyl)thio]propanedioate (3.11 g, 10.0 mmol). Melting point 201-203 °C; 1H NMR (400 MHz, DMSO-d6) δ 1.25 (d, 6H), 1.35 (t, 3H), 3.42 (m, IH), 4.35 (q, 2H), 6.94 (m, IH), 7.00 (m, 2H), 7.10 (s, 1H), 7.28 (m, 1H), 7.99 (m, 2H), 8.11 (m, 2H).

Example 180

4-Hydroxy-6-(3-hydroxyphenyl)-3-[(2-isopropylphenyl)thio]-2H-pyran-2-one

The title compound was prepared according to method A, using 3'-hydroxyacetophenone (2.06 g, 15.1 mmol), trimethylsilyl triphthalate (7.0 mL, 36.2 mmol), triethylamine (8.52 mL, 61.1 mmol), methylene chloride (30 mL), and diethyl [ (2-isopropylphenyl)thio]propanedioate (3.11 g, 10.0 mmol). Melting point 201-204 °C; 1H NMR (400 MHz, DMSO-d6) δ 1.25 (d, 6H), 3.41 (m, IH), 6.82 (2, IH), 6.93 (m, 2H), 7.09 (m, 2H), 7.30 (m, 4H), 9.91 (wide s, IH).

Example 181

4-Hydroxy-3-[(2-isopropylphenyl)thio]-2H-6-(2-phenylethyl1-ene)-pyran-2-one

The title compound was prepared according to method A, using trans-4-phenyl-3-buten-2-one (2.33 g, 15.1 mmol), trimethylsilyl triphthalate (3.5 mL, 18.1 mmol), triethylamine (4.26 mL, 30.6 mmol) methylene -chloride (30 mL) and diethyl [(2-isopropylphenyl)thio]propanedioate (3.11 g, 10.0 mmol). Melting point 190-192 °C; 1H NMR (400 MHz, DMSO-d6) δ 1.25 (d, 6H), 3.40 (m, IH), 6.44 (s, IH), 6.89 (m, IH), 7.10 (m, 3H), 7.27 (m, 1H), 7.40 (m, 4H), 7.71 (d, 2H).

Example 182

6-(1,1'-Biphen-4-yl)-4-hydroxy-3-[(2-isopropylphenyl)thio]-2H-pyran-2-one

The title compound was prepared according to method A, using 4-acetibiphenyl (3.06 g, 15.1 mmol), trimethylsilyl triphthalate (3.5 mL, 18.1 mmol), triethylamine (4.26 mL, 30.6 mmol), methylene chloride (30 mL), and diethyl [( 2-isopropylphenyl)thio]propanedioate (3.11 g, 10.0 mmol). Melting point 203-206 °C; 1H NMR (400 MHz, DMSO-d6) δ 1.26 (d, 6H). 3.40 (m, 1H), 6A4 (m, 2H), 7.10 (m, 2H), 7.28 (m, 1H), 7.51 (m, 3H), 7.77 (m, 21H), 7.91 (q, 2H).

Example 183

6-(1,1'-Biphen-3-yl)-4-hydroxy-3-[(naphthalen-2-yl)thio]-2H-pyran-2-one

The title compound was prepared according to method A, using 3'-phenylacetophenone (2 g, 10.20 mmol), trimethylsilyl triphthalate (2.27 mL, 10.20 mmol), triethylamine (2.06 g, 20.40 mmol), methylene chloride (20 mL) and diethyl [(2-naphthalen-2-yl)thio]propanedioate (1.62 g, 5.1 mmol). Melting point 183-185 °C; 1H NMR (400 MHz, DMSO-d6) δ 7.07 (s, IH), 7.33 (dd, IH), 7.39-7.58 (m, 5H), 7.66 (s, IH), 7.69 (d, IH), 7.78 ( d, 1H), 7.81-7.92 (m, 6H), 8.11 (s, 1H).

Example 184

4-Hydroxy-3-[(naphthalen-1-yl)thio]-6-phenyl-2H-pyran-2-one

The title compound was prepared according to method A, using 1-phenyl-1-(trimethylsilyloxy)ethylene (1.95 g, 10.14 mmol) and diethyl [(naphthyl)thio]propanedioate (1.61 g, 5.07 mmol). Melting point 242-243 °C; 1H NMR (400 MHz, DMSO-d6) δ 6.83 (s, IH), 7.19 (d, IH), 7.39 (t, IH), 7.64-7.42 (m, 5H), 7.69 (d, IH), 7.83 ( m, 2H), 7.94 (d, 1H), 8.28 (d, 1H).

Example 185

6-(1,1'-Biphen-3-yl)-3-[[2-(cyclopropylmethyl)phenylthio]-4-hydroxy-2H-pyran-2-one

The title compound was prepared according to method A, using 3'-phenylacetophenone (2 g, 10.20 mmol), trimethylsilyl triphthalate (2.27 mL, 10.20 mmol), triethylamine (2.06 g, 20.40 mmol), methylene chloride (20 mL) and diethyl [ [2-(cyclopropyl methyl)phenyl]thio]propanedioate (1.14 g, 5.1 mmol). Melting point 88 °C; 1H NMR (400 MHz, DMSO-d6) δ 0.25 (d, 2H), 0.53 (dd, 2H). 1.14(m, IH), 2.67 (d, 2H), 6.94 (m, IH), 7.03 (s, IH), 7.11 (m, 2H), 7.33 (m, IH), 7.44 (d, IH), 7.53 (t, 2H), 7.67 (t, 1H), 7.75 (d, 2H), 7.86 (m, 2H), 8.08 (s, 1H).

Example 186

3-[[2-(Cyclopropylmethyl)phenylthio]-6-(3,5-dimethylphenyl)-4-hydroxy-2H-pyran--2-one

The title compound was prepared according to method A, using 3,3'-dimethylacetophenone (2 g, 13.51 mmol), trimethylsilyl triphthalate (3 g, 13.1 mmol), triethylamine (2.73 g, 27.02 mmol) methylene chloride (20 mL) and diethyl [[2-(cyclopropyl methyl)phenyl]thio]propanedioate (2.18 g, 6.76 mmol). Melting point 168 °C; 1H NMR (400 MHz, DMSO-d6) δ 0.28 (d, 2H), 0.39 (dd, 2H), 1.13 (m, IH), 2.36 (s, 6H), 2.67 (d, 2H), 6.85 (s, IH), 6.92 (m, IH), 7.11 (m, 2H), 7.22 (s, IH), 7.33 (m, IH), 7.47 (s, 2H).

Example 187

6-(1,1'-Biphen-3-yl)-4-hydroxy-3-[(2-isobutylphenyl)thio]-2H-pyran-2-one

The title compound was prepared according to method A, using 3'-phenylacetophenone (2 g, 10.20 mmol), trimethylsilyl triphthalate (2.27 mL, 10.20 mmol), triethylamine (2.06 g, 20.40 mmol), methylene chloride (20 mL) and diethyl [ (2-isobutylphenyl)thio]propanedioate (1.14 g, 5.1 mmol). Melting point 187-188 °C; 1H NMR (400 MHz, DMSO-d6) δ 0.83 (d, 6H), 1.78 (m, IH), 2.4 (d, 2H), 7.03 (s, IH), 7.08 (s, 4H), 7.44 (t, 1H), 7.53 (t, 2H), 7.67 (t, 1H), 7.75 (s, 1H), 7.78 (s, 1H), 7.86 (m, 2H), 8.08 (s, 1H).

Example 188

4-Hydroxy-3-[(2-isobutylphenyl)thio]-6-phenyl-2H-pyran-2-one

The title compound was prepared according to method A, using 1-phenyl-1-(trimethylsilyloxy)ethylene (1.96 g, 10.20 mmol) and diethyl [(2-isobutylphenyl)thio]propanedioate (1.64 g, 5.1 mmol). Melting point 195 °C; 1H NMR (400 MHz, DMSO-d6) δ 0.83 (d, 6H), 1.64 (m, IH), 2.39 (d, 2H), 6.89 (s, IH), 7.06 (s, 4H), 7.56 (m, 3H), 7.86 (m, 2H).

Example 189

4-Hydroxy-3-[(2-isopropylphenyl)thio]-6-[4-(pyridin-3-yl)phenyl]-2H-pyran-2--one

The title compound was prepared according to method A, using diethyl [2-(isopropylphenyl)-thio]propanedioate (1 g, 3.22 mmol), trimethylsilyl triphthalate (1.18 g, 5.31 mmol), triethylamine (0.98 g, 4.83 mmol) and 3- (pyridin-3-yl)acetophenone (0.95 g, 4.83 mmol), as described in method A. Melting point 145-47 °C; 1H NMR (400 MHz, DMSO-d6) δ 1.25 (d, 6H), 3.4 (m, IH), 6.89 (s, IH), 6.92 (d, IH), 7.06 (m, 2H), 7.25 (d, IH), 7.53 (m, IH), 7.69 (t, IH), 7.89 (d, 2H), 8.14 (s, IH), 8.22 (d, IH), 8.61 (broad s, IH), 8.97 (broad s , IH).

Example 190

4-Hydroxy-3-[(2-isopropylphenyl)thio]-6-(3-methylphenyl)-2H-pyran-2-one

The title compound was prepared according to method A, using 3'-methylacetophenone (0.87 g, 6.46 mmol), trimethylsilyl triphthalate (1.44 g, 6.46 mmol), triethylamine (0.653 g, 6.46 mmol), methylene chloride (20 mL) and diethyl [ (2-isopropylphenyl)thio]propanedioate (1.0 g, 3.23 mmol). Melting point 161-162 °C; 1H NMR (400 MHz, DMSO-d6) δ 1.25 (d, 6H), 2.39 (s, 3H), 3.42 (m, IH), 6.86 (s, IH), 6.92 (d, IH), 7.07 (t, IH), 7.13 (t, IH), 7.28 (d, IH), 7.39 (d, IH), 7.4 (t, IH), 7.64 (d, IH), 7.67 (s, IH).

Example 191

4-Hydroxy-3-(2-isopropylphenoxy)-6-phenyl-2H-pyran-2-one

The title compound was prepared according to method A, using 1-phenyl-1-(trimethylsilyloxy)ethylene (2.62 g, 13.6 mmol) and diethyl (2-isopropyl)phenoxypropanedioate (2.0 g, 6.8 mmol). 1H NMR (250 MHz, DMSO-d6) δ 1.25 (d, 6H), 3.44 (m, IH), 6.67 (d, IH), 6.89 (s, IH), 7.0 (t, IH), 7.09 (t, 1H), 7.29 (d, 1H), 7.53 (m, 3H), 7.83 (m, 2H).

Example 192

6-(3-Chlorophenyl)-4-hydroxy-3-[(2-isopropylphenyl)thio]-2H-pyran-2-one

The title compound was prepared according to method A, using 3'-chloroacetophenone (3 g, 19.41 mmol), trimethylsilyl triphthalate (4.31 g, 19.41 mmol), triethylamine (3.92 g, 38.82 mmol), methylene chloride (20 mL) and diethyl [ (2-isopropylphenyl)thio]propanedioate (3.0 g, 9.71 mmol). Recovery of the isolated product: 70%, melting point 177-178 °C; 1H NMR (400 MHZ, DMSO-d6) δ 0.28 (d, 6H), 3.42 (m, IH), 6.92 (d, IH), 6.94 (s, IH), 7.06 (t, IH), 7.13 (t, IH), 7.28 (d, IH), 7.58 (t, IH), 7.63 (t, IH), 7.83 (d, IH), 7.89 (s, IH).

Example 193

6-(3,5-Dichlorophenyl)-4-hydroxy-3-[(2-isopropylphenyl)thio]-2H-pyran-2-one

The title compound was prepared according to method A, using 3,5-dichloroacetophenone (2 g, 10.58 mmol), trimethylsilyl triphthalate (2.35 g, 10.58 mmol), triethylamine (2.14 g, 21.16 mmol), methylene chloride (20 mL) and diethyl [(2-isopropylphenyl)thio]propanedioate (1.64 g, 5.29 mmol). Recovery of the isolated product: 70%, melting point 168-169 °C; 1H NMR (400 MHz, DMSO-d6) δ 1.25 (d, 6H), 3.42 (m, IH), 6.92 (d, IH), 7.01 (s, IH), 7.06 (t, IH), 7.13 (t, 1H), 7.28 (d, 1H), 7.83 (m, 1H), 7.88 (s, 2H).

Example 194

3-[(2,6-Dimethylphenyl)thio]-4-hydroxy-6-phenyl-2H-pyran-2-one

The title compound was prepared according to method A, using 1-phenyl-1-(trimethylsilyloxy)ethylene (1.95 g, 10.14 mmol) and diethyl [(2,6-dimethylphenyl)thio]propanedioate (2.0 g, 6.8 mmol). Melting point 248-249 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.47 (s, 6H), 6.75 (s, 1H), 7.08 (m, 3H), 7.39 (m, 3H), 7.78 (m, 2H).

Example 195

4-Hydroxy-3-[(2-methylphenyl)thio]-6-phenyl-2H-pyran-2-one

The title compound was prepared according to method A, using 1-phenyl-1-(trimethylsilyloxy)ethylene (1.95 g, 10.14 mmol) and diethyl [(2-methylphenyl)thio]propanedioate (1.43 g, 5.07 mmol). Melting point 210-211 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.47 (s, 3H), 6.82 (s, IH), 6.86 (d, IH), 7.04 (m, 2H), 7.17 (d, IH), 7.56 (m, 3H), 7.86 (m, 2H).

Example 196

3-[(2,6-Dichlorophenyl)thio]-4-hydroxy-6-phenyl-2H-pyran-2-one

The title compound was prepared according to method A, using 1-phenyl-1-(trimethylsilyloxy)ethylene (1.72 g, 8.93 mmol) and diethyl [(2,6-dichlorophenyl)thio]propanedioate (1.5 g, 4.46 mmol). Melting point 264-265 °C; 1H NMR (400 MHz, DMSO-d6) δ 6.75 (s, 1H), 7.31 (t, 1H), 7.49 (d, 2H), 7.56 (m, 3H), 7.78 (m, 2H).

Example 197

Ethyl 4-[5-(1-Cyclopentylthio-3-methylbutyl)-4-hydroxy-6-oxo-6H-pyran-2-yl]-benzoate (+/-)

The title compound was prepared according to method C, using 4-hydroxy-6-(4'-carbethoxyphenyl)-2H-pyran-2-one (1.5 g, 5.77 mmol), ethanol (15 mL), isovaletaldehyde (0.497 g, 5.77 mmol ), cyclopentylthiol (1.18 g, 11.54 mmol), piperidine (1.0 mL), and acetic acid (1.0 mL). Melting point 174-176 °C; 1H NMR (400 MHz, DMSO-d6) δ 1.05-0.72 (m, 10H), 1.82-1.14 (m, 7H), 2.13-1.81 (m, 3H), 3.04 (t, IH), 4.22 (m, IH ), 4.36 (q, 2H), 6.8 (s, IH), 7.90 (d, IH), 7.97 (q, IH), 8.15 (m, 2H).

Example 198

3-[[(Benzylthio)pyridin-3-yl]methyl]-4-hydroxy]-6-phenyl-2H-pyran-2-one (+/-)

The title compound was prepared according to method C, using 4-hydroxy-6-phenyl-2H-pyran-2-one (1.50 g, 7.98 mmol), ethanol (10 mL), pyridine-3-carboxaldehyde (0.86 g, 7.98 mmol) , benzyl mercaptan (1.98 g, 15.96 mmol), piperidine (0.5 mL), and acetic acid (0.5 mL). Melting point 103-106 °C; 1H NMR (400 MHz, DMSO-d6) δ 3.75 (q, 2H), 5.31 (s, IH), 6.33 (s, IH), 7.2 (m, IH), 7.28 (m, 5H), 7.36 (m, 3H), 7.72 (m, 2H), 7.89 (d, IH), 8.38 (dd, IH), 8.57 (s, IH).

Example 199

3-(1-Cyclopentylthio-2-cyclopropylethyl)-6-(2,3-dihydrobenzo[1,4]dioxin-6-yl)-4-hydroxy-2H-pyran-2-one (+/-)

The title compound was prepared according to method C, using 4-hydroxy-6-(2,3-dihydro-benzo[1,4]dioxin-6-yl)-2H-pyran-2-one (1.00 g, 4.06 mmol), ethanol (15 mL), cyclopropylmethylcarboxaldehyde (0.34 g, 4.06 mmol), cyclopentylthiol (0.83 g, 8.12 mmol), piperidine (1.0 mL), and acetic acid (1.0 mL). Melting point 80-82 °C; 1H NMR (400 MHz, DMSO-d6) δ 0.03 (m, 2H), 0.33 (m, 2H), 0.64 (m, 1H), 1.28-1.74 (m, 7H), -1.83-2.06 (m, 3H) , 3.06 (m, IH), 4.2 (m, IH), 4.31 (m, 4H), 6.53 (s, IH), 7.0 (d, IH), 7.24 (d, IH), 7.24 (dd, 2H).

Example 200

4-[[(4-Hydroxy-6-oxo-5-[(phenylethyl)thio]-6H-pyran-2-yl]phenoxy]methyl]-benzoic acid

In a solution of methyl 4-[[(4-hydroxy-2-oxo-3-[(2-phenylethyl)thio]-2H-pyran-2-yl]phenoxy]-methyl]benzoate (0.25 g) in dioxane (20 mL ) 2 M sodium hydroxide was added, followed by enough methanol to keep the reaction homogeneous. The reaction was stirred for 24 hours at room temperature. The solvents were evaporated. The residue was acidified with 3 M hydrochloric acid. The resulting precipitate was filtered off and washed with ether and dried in vacuum. Melting point 227 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.78 (t, 2H), 2.97 (t, 2H), 5.29 (s, 2H), 6.72 (s, 1H), 7.14-7.32 ( m, 7H), 7.58 (d, 2H), 7.78 (d, 2H), 7.97 (d, 2H).

Example 201

Ethyl 4-(4-hydroxy-6-oxo-5-[(2-phenylethyl)thio]-6H-pyran-2-yl)benzoate

The title compound was prepared according to method A, using 4-carbethoxyacetophenone (3 g, 15.61 mmol), trimethylsilyl triphthalate (3.47 g, 15.61 mmol), triethylamine (3.16 g, 31.22 mmol), methylene chloride (20 mL) and diethyl [( 2-phenylethyl)thio]propanedioate (2.31 g, 7.81 mmol). Melting point 156-158 °C; 1H NMR (400 MHz, DMSO-d6) δ 1.36 (t, 3H), 2.78 (t, 2H), 3.01 (t, 2H), 4.35 (q, 2H), 6.86 (s, 1H), 7.11-7.28 ( m, 5H), 7.92 (d, 2H), 8.08 (d, 2H).

Example 202

4-(4-Hydroxy-6-oxo-5-[(2-phenylethyl)thio]-6H-pyran-2-yl)benzoic acid

The compound ethyl 4-(4-hydroxy-6-oxo-5-[(2-phenylethyl)thio]-6H-pyran-2-yl)benzoate (0.2 g) was saponified as described in Example 200. Melting point 231 ° C; 1H NMR (400 MH/., DMSO-d6) δ 2.94 (t, 2H), 3.03 (t, 2H), 6.64 (s, 1H), 7.11-7.33 (m, 5H), 7.92 (d, 1H), 7.99 (d, IH), 8.05 (d, IH), 8.08 (d, IH).

Example 203

6-(2,3-Dihydrobenzo[1,4]dioxin-6-yl)-4-hydroxy-3-[(2-isopropylphenyl)-thio]-2H-pyran-2-one

The title compound was prepared according to method A, using 1,4-benzo[1,4]dioxin-6-yl-methyl ketone (2 g, 11.22 mmol), trimethylsilyl triphthalate (2.5 g, 11.22 mmol), triethylamine ( 2.77 g, 22.44 mmol) of methylene chloride (20 mL) and diethyl [(2-isopropylphenyl)thio]propanedioate (1.73 g, 5.61 mmol). Melting point 246-248 °C; 1H NMR (400 MHz, DMSO-d6) δ 1.25 (d, 6H), 3.4 (m, IH), 4.32 (m, 4H), 6.72 (s, IH), 6.89 (d, IH), 7.01 (d, IH), 7.06 (t, IH), 7.11 (t, IH), 7.26 (d, IH), 7.31 (d, IH), 7.35 (dd, IH).

Example 204

3-(1-Benzylthio-3-methylbutyl)-6-(2,3-dihydrobenzo[1,4]dioxin-6-yl)-4-hydroxy-2H-pyran-2-one (+/-)

The title compound was prepared according to method C, using 4-hydroxy-6-(2,3-dihydrobenzo[1,4]dioxin-6-yl)-2H-pyran-2-one (1.00 g, 4.06 mmol), ethanol ( 15 mL), isovaleraldehyde (0.35 g, 4.06 mmol), benzyl mercaptan (1.0 g, 8.12 mmol), piperidine (0.5 mL), and acetic acid (0.5 mL). 1H NMR (400 MHz, DMSO-d6) δ 0.78 (t, 6H), 1.36 (m, IH), 1.5 (m, IH), 2.06 (m, IH), 4.2 (m, IH), 4.31 (broad s , 6H), 6.56 (s, 1H), 7.03 (d, 2H), 7.36-7.25 (m, 6H).

Example 205 3-[[(Cyclohexylthio)pyridin-4-yl]methyl]-4-hydroxy-6-phenyl-2H-pyran-2-one-(+/-)

The title compound was prepared according to method C, using 4-hydroxy-6-phenyl-2H-pyran-2-one (1.5 g, 7.98 mmol), ethanol (10 mL), pyridine-4-carboxaldehyde (0.86 g, 7.98 mmol) , cyclohexylthiol (1.86 g, 7.98 mmol), piperidine (0.5 mL), and acetic acid (0.5 mL). 1H NMR (400 MHz, DMSO-d6) δ 1.25 (m, 5H), 1.53 (m, IH), 1.67 (m, 2H), 1.92 (m, 2H), 2.71 (m, IH), 5.33 (s, 1H), 6.69 (s, 1H), 7.5 (m, 5H), 7.75 (m, 2H), 8.47 (d, 2H).

Example 206 3-[[(Cyclohexylthio)pyridin-3-yl]methyl]-4-hydroxy-6-phenyl-2H-pyran-2-one-(+/-)

The title compound was prepared according to method C, using 4-hydroxy-6-phenyl-2H-pyran-2-one (1.5 g, 7.98 mmol), ethanol (10 mL), pyridine-3-carboxaldehyde (0.86 g, 7.98 mmol) , cyclohexylthiol (1.86 g, 7.98 mmol), piperidine (0.5 mL), and acetic acid (0.5 mL). 1H NMR (400 MHz, DMSO-d6) δ 1.25 (m, 5H), 1.53 (m, IH), 1.67 (m, 2H), 1.92 (m, 2H), 2.69 (m, IH), 5.39 (s, IH), 6.72 (s, IH), 7.33 (m, IH), 7.53 (m, 3H), 7.73 (m, 2H), 7.97 (d, IH), 8.39 (d, IH), 8.67 (d, IH ).

Example 207

4-[[(4-Hydroxy-2-oxo-6-phenyl-2H-pyran-3-yl)thio]methyl]benzoic acid

The title compound was prepared by saponification of methyl 4-[[(4-hydroxy-2-oxo-6-phenyl-2H--pyran-3-yl)thio]methyl]benzoate (0.1 g) as described in Example 200. Melting point 231 °C; 1H NMR (400 MHz, DMSO-d6) δ 4.06 (s, 2H), 6.72 (s, 1H), 7.36 (d, 2H), 7.58 (m, 3H), 7.86 (m, 4H).

Example 208

3-[[(4-Hydroxy-2-oxo-6-phenyl-2H-pyran-3-yl)thio]methyl]benzoic acid

The title compound was prepared by saponification of methyl 3-[[(4-hydroxy-2-oxo-6-phenyl-2H--pyran-3-yl)thio]methyl]benzoate (0.1 g) as described in Example 200. 1H NMR (400 MHz, DMSO-d6) δ 4.4 (d, IH), 4.72 (d, IH), 6.72 (s, IH), 7.4 (t, IH), 7.44 7.61 (m, 5H), 7.74-7.92 (m , 4H).

Example 209

2-[[(4-Hydroxy-2-oxo-6-phenyl-2H-pyran-3-yl)thio]methylbenzoic acid

The title compound was prepared by saponification of methyl 2-[[(4-hydroxy-2-oxo-6-phenyl-2H--pyran-3-yl)thio]methyl]benzoate (0.2 g) as described in Example 200. Melting point 231 °C; 1H NMR (400 MHz, DMSO-d6) δ 4.36 (s, 2H), 6.69 (s, IH), 7.18 (d, IH), 7.29 (t, IH), 7.39 (t, IH), 7.53 (m, 3H), 7.79 (m, 3H).

Example 210

3-[(2-Chlorophenyl)thio]-4-hydroxy-6-phenyl-2H-pyran-2-one

The title compound was prepared according to method A, using 1-phenyl-1-(trimethylsilyloxy)ethylene (1.95 g, 10.14 mmol) and diethyl [(2-chlorophenyl)thio]propanedioate (1.53 g, 5.07 mmol). Melting point 275-280 °C; 1H NMR (400 MHz, DMSO-d6) δ 6.78 (s, IH), 6.89 (dd, IH), 7.08 (tt, IH), 7.19 (tt, IH), 7.42 (dd, IH), 7.56 (m, 3H), 8.06 (m, 2H).

Example 211

4-Hydroxy-3-[(2-methoxyphenyl)thio]-6-phenyl-2H-pyran-2-one

The title compound was prepared according to method A, using 1-phenyl-1-(trimethylsilyloxy)ethylene (1.95 g, 10.14 mmol) and diethyl [(2-methoxyphenyl)thio]propanedioate (1.51 g, 5.07 mmol). Melting point 208-209 °C; 1H NMR (250 MHz, DMSO-d6) δ 3.83 (s, 3H), 6.74 (dd, IH), 6.82 (d, IH), 6.86 (s, IH), 6.97 (d, IH), 7.08 (t, 1H), 7.56 (m, 3H), 7.86 (m, 2H).

Example 212

6-(4-Benzyloxyphenyl)-4-hydroxy-3-[(2-isopropylphenyl)thio]-2H-pyran-2-one

The title compound was prepared according to method A, using 4-benzyloxyacetophenone (0.3 g, 0.675 mmol), trimethylsilyl triphthalate (0.15 g, 0.675 mmol), triethylamine (0.14 g, 1.35 mmol), methylene chloride (20 mL) and diethyl [( 2-isopropylphenyl)thio]propanedioate (0.210 g, 0.675 mmol). Melting point 163-165 °C; 1H NMR (400 MHz, DMSO-d6) δ 1.28 (d, 6H), 3.4 (m, IH), 5.22 (s, 2H), 6.64 (s, IH), 6.92 (d, IH), 7.06 (t, IH), 7.11 (t, IH), 7.19 (d, IH), 7.28 (d, IH), 736 (q, 2H), 7.42 (d, 2H), 7.49 (d, 2H), 7.83 (d, 2H ).

Example 213

3-[(3-Chlorophenyl)thio]-4-hydroxy-6-phenyl-2H-pyran-2-one

The title compound was prepared according to method A, using 1-phenyl-1-(trimethylsilyloxy)ethylbenzene (1.95 g, 10.14 mmol) and diethyl [(3-chlorophenyl)thio]propanedioate (1.53 g, 5.07 mmol). Melting point 181-182 °C; 1H NMR (400 MHz, DMSO-d6) δ 6.88 (s, IH), 7.13 (dt, 2H), 7.19 (dt, IH), 7.29 (t, IH), 7.56 (m, 3H), 7.86 (m, 2H).

Example 214

4-Hydroxy-3-[(3-methoxyphenyl)thio]-6-phenyl-2H-pyran-2-one

The title compound was prepared according to method A, using 1-phenyl-1-(trimethylsilyloxy)ethylene (1.95 g, 10.14 mmol) and diethyl [(3-methoxyphenyl)thio]propanedioate (1.51 g, 5.07 mmol). Melting point 130-131 °C; 1H NMR (400 MHz, DMSO-d6) δ 3.69 (s, 3H), 6.69 (dd, IH), 6.72 (dd, IH), 6.89 (s, IH), 7.2 (dt, 2H), 7.58 (m, 3H), 7.88 (m, 2H).

Example 215

4-Hydroxy-3-[(3-methylphenyl)thio]-6-phenyl-2H-pyran-2-one

The title compound was prepared according to method A, using 1-phenyl-1-(trimethylsilyloxy)ethylene (1.95 g, 10.14 mmol) and diethyl [(3-methylphenyl)thio]propanedioate (1.43 g, 5.07 mmol). Melting point 197-198 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.24 (s, 3H), 6.89 (s, IH), 6.93 (t, IH), 6.97 (s, IH), 7.14 (t, 2H), 7.56 (m, 3H), 7.86 (m, 2H).

Example 216

3-[(2-Ethylphenyl)thio]-4-hydroxy-6-phenyl-2H-pyran-2-one

The title compound was prepared according to method A, using 1-phenyl-1-(trimethylsilyloxy)ethylbenzene (4.17 g, 21.72 mmol) and diethyl [(2-ethylphenyl)thio]propanedioate (1.5 g, 10.86 mmol). Melting point 190-192 °C; 1H NMR (400 MHz, DMSO-d6) δ 1.25 (t, 3H), 2.78 (q, 2H), 6.89 (s, IH), 6.92 (m, IH), 7.08 (m, 2H), 7.2 (m, 1H), 7.58 (m, 3H), 7.86 (m, 2H).

Example 217

3-[(2-Isopropylphenyl)thio]-2-oxo-6-phenyl-2H-pyran-4-yl acetate

This compound was prepared by the reaction of the sodium salt of 4-hydroxy-3-[(2-isopropylphenyl)]thio]-6-phenyl-2H-pyran-2-one (0.2 g, 0.59 mmol) and acetyl chloride (0.09 g, 1.18 mmol), as described in general procedure G. Melting point 113-115 °C; 1H NMR (400 MHz, DMSO-d6) δ 1.26 (d, 6H), 1.89 (s, 3H), 3.42 (m, IH), 6.89 (s, IH), 6.94 (dd, IH), 7.04 (dt, IH), 7.13 (dt, IH), 7.28 (dd, IH), 7.58 (m, 3H), 7.86 (m, 2H).

Example 218

4-Hydroxy-6-phenyl-3-[(3-trifluoromethylphenyl)thio]-2H-pyran-2-one

The title compound was prepared according to method A, using 1-phenyl-1-(trimethylsilyloxy)ethylene (1.72 g, 8.92 mmol) and diethyl [[3-(trifluoromethyl)phenyl]thio]propanedioate (1.5 g, 4.46 mmol). Melting point 228-229 °C; 1H NMR (400 MHz, DMSO-d6) δ 6.89 (s, 1H), 7.4-7.61 (m, 7H), 7.89 (m, 2H).

Example 219

3-[(3,5-Dimethylphenyl)thio]-4-hydroxy-6-phenyl-2H-pyran-2-one

The title compound was prepared according to method A, using 1-phenyl-1-(trimethylsilyloxy)ethylene (1.3 g, 6.76 mmol) and diethyl [(3,5-dimethyl)phenyl]thio]propanedioate (1.0 g, 3.38 mmol). Melting point 214-216 °C; 1H NMR (400 MHz, DMSO-d6) δ 2.2 (s, 6H), 6.75 (broad s, 3H), 6.89 (s, 1H), 7.56 (m, 3H), 7.86 (m, 2H).

Example 220

6-[4-(Cyclohexylmethoxy)phenyl]-4-hydroxy-3-[(2-isopropylphenyl)thio]-2H-pyran-2-one

The title compound was prepared according to method A, using 4-cyclohexylmethoxyacetophenone (2.0 g, 8.61 mmol), trimethylsilyl triphthalate (1.91 g, 8.61 mmol), triethylamine (1.74 g, 17.22 mmol), methylene chloride (20 mL) and diethyl [ (2-isopropylphenyl)thio]propanedioate (4.0 g, 12.92 mmol). Melting point 187-188 °C; 1H NMR (400 MHz, DMSO-d6) δ 0.96-1.33 (m+1.24 d, UH), 1.61-1.87 (m, 6H), 3.4 (m, IH), 3.86 (d, 2H), 6.76 (s, IH), 6.92 (dd, IH), 7.11 (m, 4H), 7.29 (dd, IH), 7.81 (d, 2H).

Example 221

6-(3-Benzyloxyphenyl)-4-hydroxy-3-[(2-isopropylphenyl)thio]-2H-pyran-2-one

The title compound was prepared according to method A, using 3-benzyloxyacetophenone (2.0 g, 8.84 mmol), trimethylsilyl triphthalate (1.96 g, 8.84 mmol), triethylamine (1.79 g, 17.68 mmol) and diethyl [(2-isopropylphenyl)thio]propanedioate. (0.210 g, 0.675 mmol). Melting point 162-164 °C; 1H NMR (400 MHz, DMSO-d6) δ 1.25 (d, 6H), 3.42 (m, IH), 5.35 (s, 2H), 6.89 (s, IH), 6.92 (d, IH), 7.06 (dt, IH), 7.11 (dt, IH), 7.22 (dd, IH), 7.28 (dd, IH), 7.39-7.51 (m, 8H).

Example 222

4-Hydroxy-3-[(2-isopropylphenyl)thio]-6-[4-(3-phenylpropoxy)phenyl]-2H--pyran-2-one

The title compound was prepared according to method A, using 4-phenylpropyloxyacetophenone (2.0 g, 7.86 mmol), trimethylsilyl triphthalate (1.75 g, 7.86 mmol), triethylamine (1.59 g, 15.72 mmol) and diethyl [(2-isopropylphenyl)thio]propanedioate. (3.66 g, 11.79 mmol). Melting point 132-133 °C; 1H NMR (400 MHz, DMSO-d6) δ 1.25 (d, 6H), 2.06 (m, 2H), 2.75 (t, 2H), 3.39 (m, 1H), 4.08 (t, 2H), 6.78 (s, 1H), 6.90 (d, 1H), 7.05 (dt, 2H), 7.08 (q, 2H), 7.11-7.31 (m, 6H), 7.81 (d, 2H).

Example 223

3-[(2-sec-Butylphenyl)thio]-4-hydroxy-6-phenyl-2H-pyran-2-one (+/-)

The title compound was prepared according to method A, using 1-phenyl-1-(trimethylsilyloxy)ethylene (1.0 g, 6.17 mmol) and diethyl [(2-sec-butylphenyl)thio]propanedioate (1.0 g, 3.09 mmol). Melting point 170-171 °C; 1H NMR (400 MHz, DMSO-d6) δ 0.86 (t, 3H), 1.22 (d, 3H), 1.57 (m, IH), 1.67 (m, IH), 3.22 (m, IH), 6.89 (s, 1H), 6.97 (d, 1H), 7.06 (t, 1H), 7.13 (t, 1H), 7.22 (d, 1H), 7.56 (m, 3H), 7.86 (m, 2H).

4.5. Determination of HIV protease inhibition

4.5.1. Starting substances

DTT buffer: 1.0 mM dithiothreitol (DTT) was prepared fresh every day in 0.1% polyethylene glycol (Mr 8000), 80 mM NaOAc, 160 mM NaCl, 1.0 mM EDTA and titrated to pH 4.7 with HCl.

HIV-1 Protease: Enzyme was obtained from Bachem Bioscience Inc. Undiluted enzyme was thawed at -80 °C and diluted 50-fold with DTT buffer. The solution was always kept at 0 °C in ice water and used in the experiment within 20 min after thawing.

Enzyme substrate: Substrate III obtained from Bachem Bioscience Inc. is an undecapeptide H-His-Lys-Ala-Arg-Val-Leu-p-Nitrophenylalanine-Glu-Ala-Norleucine-Ser-NH2 (purity >97%). A stock solution of 200 μM in DTT buffer is prepared and stored on ice. A fresh substrate solution is prepared daily.

Test compound: 10 mM inhibitor (I) in dimethyl sulfoxide (DMSO) is diluted to a concentration of 200 µM in DTT buffer. A 10 μM stock solution with 2% DMSO in DTT buffer was made from a 200 μM stock solution. Two inhibitor solutions are used to prepare final solutions of [I] = 100, 50, 20, 10, 5, 2, 1, 0.5 and 0 μM with 2% DMSO in DTT buffer in each reaction well. (total inhibitor volume 50 μL)

4.5.2. Determination procedure

20 μL of substrate (final concentration 40 μM), 50 μL of inhibitor (in such a concentration that the final dilution leads to the desired tested concentration) and 20 μL of DTT buffer are added to each reaction well. Reaction plates (96 wells) are incubated at 37 °C for at least 5 minutes.

10 μL of the diluted protease solution is added to the well of the reaction plate with constant shaking. After shaking for 10 seconds, the plates are transferred to a cell heating block at 37°C. (the final reaction volume is 100 μL.)

The reaction is incubated for 5 minutes at 37 °C. The reaction is stopped by placing the reaction plate on a shaker and adding 20 μL of 10% trifluoroacetic acid (TFA) and shaking for 10 seconds. The intensity of proteolysis is determined by separating the undegraded substrate and the two degradation products using reverse-phase high-pressure liquid chromatography, and by measuring the absorbance at 220 nm, so that the relative areas of the signals corresponding to individual components are determined. The relative signal areas are used to calculate the % conversion of substrates to products as a function of the concentration of the applied inhibitor. Data are presented as % of the control (ratio of % conversion in the presence and absence of the inhibitor x 100) in relation to the concentration of the inhibitor, and were tested according to the equation: y= 100/ l+(X/IC50)A where IC50 is the concentration of the inhibitor at 50% inhibition, and A slope of the inhibition curve.

The results are shown in Table I.

[image]

[image]

4. 6. Anti-HIV-1 activity

By applying general methods according to Pauwels et al., (J. Virol. Methods, 16, 171-185, 1987) and according to Mann et al. (AIDS Research and Human Retroviruses, 253-255, 1989 (anti-viral assays for acute infections by HIV-1 were performed on the H9 cell line) Cultures were pooled in 1 mL RPM1 1640 medium/10% fetal calf serum containing 105 infectious doses of HlVl^b for an effective multiplicity of infection of 0.01. Two hours after virus absorption, the cells were washed once and plated on 96-well microtiter plates at a density of 104 cells per well. The compounds to be tested were added to obtain the desired concentration drug and 0.1% DMSO in a final volume of 200 μL. Comparative uninfected cultures were used for XTT cytotoxicity assays 7 days after infection. Cultures were tested for the degree of viral replication by reverse transcriptase at 4 and 7 days after infection.

[image]

Combinations of protease inhibitors with other anti-AIDS treatments, such as (but not limited to) reverse transcriptase inhibitors AZT or ddC, can achieve synergistic effects. J.C. Craig et al., Antiviral Chem. Chemother., 38: 348-352 (1994); DM Lambert et al., Antiviral Res., 21: 327-342 (1993); AM Caliendo et al., Clin. Infect. Dis 18/4: 516-524 (1994).

The compounds of this invention show antibacterial activity when tested by the microtitration dilution method, as described in Heifetz et al., Antimicr. Agents. & Chemoth. 6: 124 (1974) which is incorporated herein by reference.

Using the above comparative method, the following minimum inhibitory concentrations (MICs in μg/mL) were obtained for representative compounds from the invention against clinically relevant gram-positive pathogens that have become highly resistant to conventional therapy in recent years.

[image]

It is obvious to an expert that other combinations, which are not specifically mentioned in this report, were also considered in comparison. Such other combinations are considered to be within the scope and spirit of the invention. Therefore, it should not be understood that the invention is limited by the description of the specific parts described so far, but is limited only by the stated patent claim.

Claims (30)

1. A compound or a pharmaceutically acceptable salt of the formula [image] indicated that X represents OR 1 , NHR 1 , SR 1 , CO 2 R 4 , CH 2 OR 1 , wherein R 1 represents R 4 or COR 4 , and R 4 is as defined below; Y represents oxygen or sulfur; Z represents rain or sulphur; A and A1 are independently chemical bonds, unsubstituted or substituted phenyl, naphthyl, five- or six-membered heterocyclic ring, cycloalkyl or fused ring system of 8 to 10 atoms or their substituted derivative, indicated that the substituents can be one or more of the following groups: F, Cl, Br, OR4, N(R4)2, CO2R4, CO2N(R4)2, COR4, R4, OCH2O, OCH2CH2O or C≡N, where R4 is independently hydrogen, substituted or unsubstituted alkyl, cycloalkyl, alkylcycloalkyl or phenyl , where the substituents can be one or more of the following groups: CO2R2, CON(R2)2, F, OR2, SR2, N(R2)2, CN, phenyl, naphthyl, heterocyclyl or CF3, and R2 independently represents alkyl, cycloalkyl or hydrogen; R5 represents hydrogen, alkyl, cycloalkyl, alkylcycloalkyl, phenyl or their substituted derivative, and the substituents can be one or more of the following groups: CO2R2, CON(R2)2, F, OR2, phenyl, naphthyl, CF3, OR1, NHR1, SR1 , or CH 2 OR 1 , wherein R 1 is as defined above; R3 is independently hydrogen, (CH2)pR4 or (CH2)pA where p is an integer from 0 to 2 and R4 and A are as defined above; W, W1, and W4 each independently represent a chemical bond, oxygen, NR3, C(R3)2, CO, CR3=CR3, C≡C, OR3OR3, C(=NR3)NR3, S(O)p, CR3N(R3 )2, SO2NR3, CO2, NR3COVgA, and NCOVgR3, indicated that g is 0 or 1, and V can be oxygen, sulfur, NR3 or CHR3; W2 represents oxygen, NR3, S(O)p, SO2NR3, -OCO, NR3COVgA, and NCOVgR3, where g is 0 or 1, and V can be oxygen, sulfur, NR3 or CHR3; m and n are each independently integers from 0 to 4 with the restriction that if both W and W1 are heteroatoms, or if both W2 and W3 are heteroatoms, m is an integer from 2 to 4; and with the further restriction that R3W1(CH2)mW(CH2)nA cannot be methyl or ethyl. 2. Compound of the formula from claim 1, indicated that W2 represents SO2NR3 or S(O)p, and p is an integer from 0 to 2. 3. A compound of the formula from claim 2, characterized in that A1 does not represent a bond. 4. Compound of the formula from patent claim 3, characterized in that it is selected from the following group of compounds: [image] [image] [image] [image] [image] [image] 5. Compound of the formula from claim 2, characterized in that A1 represents a bond, and the group (CH2)W3 viewed together does not represent a bond. 6. Compound of the formula from patent claim 5, characterized in that it is selected from the following group of compounds: [image] [image] [image] [image] [image] 7. A compound of the formula from claim 1, characterized in that W2 represents oxygen. 8. A compound of the formula from claim 7, characterized in that A1 does not represent a bond. 9. Compound of the formula from patent claim 8, characterized in that it is selected from the following group of compounds: [image] [image] 10. Compound of the formula from claim 7, characterized in that A1 represents a bond, and the group (CH2)W3 viewed together does not represent a bond. 11. A compound of the formula from patent claim 10, characterized in that it is selected from the following group of compounds: [image] 12. Compound of the formula from claim 1, characterized in that W2 is selected from the following groups: NR3; NR3COVgA; NCOVgR3 or SO2NR, with the nitrogen attached in the 3-position to the pyrone ring at any choice of W2. 13. Compound of the formula from claim 12, characterized in that W2 represents NR3. 14. Compound of the formula from claim 13, characterized in that it is selected from the following group of compounds: [image] [image] 15. Compound of the formula from claim 12, characterized in that W2 is selected from the following groups: NR3COVgA; NCOVgR3 or SO2NR, with the nitrogen attached in the 3-position to the pyrone ring at any choice for W2. 16. A compound of the formula from patent claim 15, characterized in that it is selected from the following group of compounds: [image] 17. Name compounds: [image] 18. Name compounds: [image] 19. A pharmaceutical preparation for the treatment of an infection or disease caused by a certain strain of bacteria, characterized by the fact that it contains a sufficient amount of the compound from claim 1 necessary to ensure the antibacterial effect of the dose of the compound, which ranges from about 1 to about 50 mg/kg per day, together with a pharmaceutically acceptable excipient. 20. A pharmaceutical composition for the treatment of an infection or disease caused by a retrovirus, characterized in that it contains a sufficient amount of the compound of claim 1 necessary to ensure the antiviral effect of a dose of the compound, ranging from about 1 to about 50 mg/kg per day, together with a pharmaceutically effective auxiliary agent. 21. A pharmaceutical composition for the treatment of an infection or disease caused by a retrovirus, characterized in that it contains a sufficient amount of the compound of claim 2 necessary to ensure the antiviral effect of a dose of the compound, ranging from about 1 to about 50 mg/kg per day, together with a pharmaceutically effective auxiliary agent. 22. A pharmaceutical preparation for the treatment of an infection or disease caused by a retrovirus, characterized in that it contains a sufficient amount of the compound of claim 4 necessary to ensure the antiviral effect of a dose of the compound, which ranges from about 1 to about 50 mg/kg per day, together with a pharmaceutically effective auxiliary agent. 23. A method of treating an infection or disease caused by a retrovirus, indicated by the fact that it comprises administering the preparation from patent claim 1 to a person in need of such treatment. 24. A method of treating an infection or a disease caused by a retrovirus, characterized in that it comprises administering the preparation from claim 1 in combination with an inhibitor of HlV-virus reverse transcriptase to a person in need of such treatment. 25. A method of treating an infection or disease caused by a retrovirus, characterized in that it comprises administering the preparation from claim 1 in combination with AZT to a person in need of such treatment. 26. A method of treating an infection or disease caused by a retrovirus, characterized by the fact that it comprises the administration of the preparation from patent claim 1 in combination with ddC to a person in need of such treatment. 27. A method of treating an infection or disease caused by a retrovirus, indicated by the fact that it comprises administering the preparation from patent claim 2 to a person in need of such treatment. 28. A method of treating an infection or disease caused by a retrovirus, characterized in that it comprises administering the preparation from claim 2 in combination with an inhibitor of reverse transcriptase of the HIV virus, to a person in need of such treatment. 29. A method of treating an infection or disease caused by a retrovirus, characterized by the fact that it comprises administering the preparation from patent claim 4 to a person in need of such treatment. 30. A method of treating an infection or disease caused by a retrovirus, characterized by the fact that it comprises the administration of the preparation from claim 4 in combination with an inhibitor of reverse transcriptase of the HIV virus, to a person in need of such treatment.