HRP20240014T1 - Hplc-bazirana detekcija sredstava za flokulaciju u uzorku proteina - Google Patents
Hplc-bazirana detekcija sredstava za flokulaciju u uzorku proteina Download PDFInfo
- Publication number
- HRP20240014T1 HRP20240014T1 HRP20240014TT HRP20240014T HRP20240014T1 HR P20240014 T1 HRP20240014 T1 HR P20240014T1 HR P20240014T T HRP20240014T T HR P20240014TT HR P20240014 T HRP20240014 T HR P20240014T HR P20240014 T1 HRP20240014 T1 HR P20240014T1
- Authority
- HR
- Croatia
- Prior art keywords
- sample
- pdadmac
- cad
- acid
- hplc
- Prior art date
Links
- 102000004169 proteins and genes Human genes 0.000 title claims 3
- 108090000623 proteins and genes Proteins 0.000 title claims 3
- 238000001514 detection method Methods 0.000 title 1
- 230000016615 flocculation Effects 0.000 title 1
- 238000005189 flocculation Methods 0.000 title 1
- 238000000034 method Methods 0.000 claims 13
- 229920000371 poly(diallyldimethylammonium chloride) polymer Polymers 0.000 claims 6
- 239000002253 acid Substances 0.000 claims 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims 3
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 claims 3
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 claims 3
- 238000004128 high performance liquid chromatography Methods 0.000 claims 3
- 230000002209 hydrophobic effect Effects 0.000 claims 3
- DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 claims 2
- 238000010828 elution Methods 0.000 claims 2
- 230000003993 interaction Effects 0.000 claims 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 claims 2
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims 1
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims 1
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims 1
- 239000000443 aerosol Substances 0.000 claims 1
- 235000019253 formic acid Nutrition 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 238000005259 measurement Methods 0.000 claims 1
- YPJUNDFVDDCYIH-UHFFFAOYSA-N perfluorobutyric acid Chemical compound OC(=O)C(F)(F)C(F)(F)C(F)(F)F YPJUNDFVDDCYIH-UHFFFAOYSA-N 0.000 claims 1
- 238000001742 protein purification Methods 0.000 claims 1
- 238000000746 purification Methods 0.000 claims 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/16—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
- B01D15/166—Fluid composition conditioning, e.g. gradient
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/32—Bonded phase chromatography
- B01D15/325—Reversed phase
- B01D15/327—Reversed phase with hydrophobic interaction
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
- G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
- G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Claims (10)
1. Postupak za mjerenje pDADMAC u uzorku koji sadrži rekombinantni protein od interesa, te postupak obuhvaća sljedeće korake:
a) primjenu uzorka na HPLC hidrofobne interakcije reverzne faze,
b) eluiranje vezanog pDADMAC iz kolone korištenjem hidrofobnog gradijenta eluiranja,
c) mjerenje pDADMAC dobivenog u koraku b) pomoću detektora nabijenog aerosola (CAD),
naznačen time što uzorak primijenjen u a) sadrži 0,2-3% (v/v) CAD-kompatibilne kiseline.
2. Postupak prema zahtjevu 1, naznačen time što uzorak primijenjen u koraku a) sadrži koncentraciju proteina manju od 200 mg/ml, kao što je 20-100 mg/ml, npr. 20-60 mg/ml.
3. Postupak prema zahtjevu 1 ili 2, naznačen time što uzorak primijenjen u koraku a) ima pH od 2 do 4.
4. Postupak prema bilo kojem od prethodnih zahtjeva, naznačen time što uzorak primijenjen u koraku a) sadrži 0,5-1,5% (v/v) CAD-kompatibilne kiseline.
5. Postupak prema bilo kojem od prethodnih zahtjeva, naznačen time što je navedena CAD-kompatibilna kiselina odabrana od trifluoroctene kiseline, octene kiseline, heptafluormaslačne kiseline i mravlje kiseline.
6. Postupak prema bilo kojem od prethodnih zahtjeva, naznačen time što HPLC hidrofobne interakcije reverzne faze je PLRP-S HPLC.
7. Postupak prema bilo kojem od prethodnih zahtjeva, naznačen time što postupak nadalje obuhvaća korak određivanja koncentracije pDADMAC prisutnog u uzorku usporedbom s kontrolom.
8. Postupak prema bilo kojem od prethodnih zahtjeva, naznačen time što je rekombinantni protein od interesa protutijelo, fragment protutijela ili njegov derivat.
9. Postupak prema bilo kojem od prethodnih zahtjeva, naznačen time što je uzorak primijenjen u koraku a) uzorak dobiven izravno iz koraka u pročišćavanju navedenog rekombinantnog proteina kojem je dodana CAD-kompatibilna kiselina.
10. Postupak proizvodnje proteina od interesa koji uključuje dodavanje pDADMAC tijekom pročišćavanja proteina, naznačen time što se naknadno uklanjanje navedenog pDADMAC mjeri postupkom prema bilo kojem od zahtjeva 1 do 9.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP19181523 | 2019-06-20 | ||
| PCT/EP2020/066922 WO2020254483A1 (en) | 2019-06-20 | 2020-06-18 | Hplc-based detection of flocculation agents in a protein sample |
| EP20733284.2A EP3986588B1 (en) | 2019-06-20 | 2020-06-18 | Hplc-based detection of flocculation agents in a protein sample |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20240014T1 true HRP20240014T1 (hr) | 2024-03-29 |
Family
ID=67220626
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20240014TT HRP20240014T1 (hr) | 2019-06-20 | 2020-06-18 | Hplc-bazirana detekcija sredstava za flokulaciju u uzorku proteina |
Country Status (20)
| Country | Link |
|---|---|
| US (1) | US20220241701A1 (hr) |
| EP (1) | EP3986588B1 (hr) |
| JP (1) | JP2022537297A (hr) |
| KR (1) | KR20220024577A (hr) |
| CN (1) | CN113924153B (hr) |
| AR (1) | AR120062A1 (hr) |
| AU (1) | AU2020294922A1 (hr) |
| BR (1) | BR112021021212A2 (hr) |
| CA (1) | CA3138832A1 (hr) |
| DK (1) | DK3986588T3 (hr) |
| FI (1) | FI3986588T3 (hr) |
| HR (1) | HRP20240014T1 (hr) |
| IL (1) | IL289021A (hr) |
| LT (1) | LT3986588T (hr) |
| MX (1) | MX2021015831A (hr) |
| PL (1) | PL3986588T3 (hr) |
| PT (1) | PT3986588T (hr) |
| SG (1) | SG11202112000YA (hr) |
| SI (1) | SI3986588T1 (hr) |
| WO (1) | WO2020254483A1 (hr) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4236099A1 (en) | 2022-02-24 | 2023-08-30 | Samsung Electronics Co., Ltd. | Apparatus and method for channel sounding |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1007906B (zh) * | 1983-11-28 | 1990-05-09 | 武田药品工业株式会社 | 高纯度蛋白质的制备及其应用 |
| US6673629B2 (en) * | 1998-01-15 | 2004-01-06 | Abbott Laboratories | Neutralization of polycations in a chromatographic device for whole blood use |
| ES2361925T3 (es) * | 2000-08-25 | 2011-06-24 | Basf Plant Science Gmbh | Polinucleótidos de plantas que codifican prenil proteasas. |
| EP2358734A1 (en) * | 2008-12-16 | 2011-08-24 | Millipore Corporation | Purification of proteins |
| CN103120864B (zh) * | 2013-03-06 | 2015-04-08 | 苏州环球色谱有限责任公司 | 一种石墨烯修饰毛细管色谱柱的制备方法 |
| CA2924821A1 (en) * | 2013-09-20 | 2015-03-26 | Davuluri, Ramamohan Rao | Purification of organic compounds by surfactant mediated preparative hplc |
| GB201411320D0 (en) | 2014-06-25 | 2014-08-06 | Ucb Biopharma Sprl | Antibody construct |
| KR102450255B1 (ko) * | 2016-05-25 | 2022-10-04 | 론자 휴스턴 아이엔씨. | 폴리디알릴 디알킬암모늄 염을 이용한 아데노-관련 바이러스의 분리방법 |
| US10927145B2 (en) * | 2016-06-15 | 2021-02-23 | Agency For Science, Technology And Research | Method of enhancing the performance of chromatography methods for purification of proteins |
-
2020
- 2020-06-18 DK DK20733284.2T patent/DK3986588T3/da active
- 2020-06-18 BR BR112021021212A patent/BR112021021212A2/pt unknown
- 2020-06-18 AU AU2020294922A patent/AU2020294922A1/en active Pending
- 2020-06-18 HR HRP20240014TT patent/HRP20240014T1/hr unknown
- 2020-06-18 WO PCT/EP2020/066922 patent/WO2020254483A1/en active Application Filing
- 2020-06-18 JP JP2021574906A patent/JP2022537297A/ja active Pending
- 2020-06-18 EP EP20733284.2A patent/EP3986588B1/en active Active
- 2020-06-18 PL PL20733284.2T patent/PL3986588T3/pl unknown
- 2020-06-18 US US17/620,137 patent/US20220241701A1/en active Pending
- 2020-06-18 SG SG11202112000YA patent/SG11202112000YA/en unknown
- 2020-06-18 FI FIEP20733284.2T patent/FI3986588T3/fi active
- 2020-06-18 CA CA3138832A patent/CA3138832A1/en active Pending
- 2020-06-18 LT LTEPPCT/EP2020/066922T patent/LT3986588T/lt unknown
- 2020-06-18 SI SI202030359T patent/SI3986588T1/sl unknown
- 2020-06-18 MX MX2021015831A patent/MX2021015831A/es unknown
- 2020-06-18 CN CN202080038440.XA patent/CN113924153B/zh active Active
- 2020-06-18 KR KR1020227001402A patent/KR20220024577A/ko unknown
- 2020-06-18 PT PT207332842T patent/PT3986588T/pt unknown
- 2020-06-19 AR ARP200101729A patent/AR120062A1/es unknown
-
2021
- 2021-12-15 IL IL289021A patent/IL289021A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| BR112021021212A2 (pt) | 2021-12-28 |
| CN113924153B (zh) | 2023-08-01 |
| SI3986588T1 (sl) | 2024-03-29 |
| EP3986588B1 (en) | 2023-11-08 |
| DK3986588T3 (da) | 2024-01-15 |
| FI3986588T3 (fi) | 2024-01-19 |
| SG11202112000YA (en) | 2021-11-29 |
| US20220241701A1 (en) | 2022-08-04 |
| PL3986588T3 (pl) | 2024-04-08 |
| MX2021015831A (es) | 2022-02-03 |
| CN113924153A (zh) | 2022-01-11 |
| KR20220024577A (ko) | 2022-03-03 |
| LT3986588T (lt) | 2024-02-12 |
| AU2020294922A1 (en) | 2021-11-18 |
| CA3138832A1 (en) | 2020-12-24 |
| IL289021A (en) | 2022-02-01 |
| PT3986588T (pt) | 2024-01-18 |
| AR120062A1 (es) | 2022-02-02 |
| WO2020254483A1 (en) | 2020-12-24 |
| JP2022537297A (ja) | 2022-08-25 |
| EP3986588A1 (en) | 2022-04-27 |
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