HRP20231499T1 - Ex-vivo sustav kulture i metode korištenja istog - Google Patents

Ex-vivo sustav kulture i metode korištenja istog Download PDF

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HRP20231499T1
HRP20231499T1 HRP20231499TT HRP20231499T HRP20231499T1 HR P20231499 T1 HRP20231499 T1 HR P20231499T1 HR P20231499T T HRP20231499T T HR P20231499TT HR P20231499 T HRP20231499 T HR P20231499T HR P20231499 T1 HRP20231499 T1 HR P20231499T1
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Croatia
Prior art keywords
tissue
culture
drug
culture system
culture medium
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HRP20231499TT
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Ravid STRAUSSMAN
Nancy GAVERT
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Yeda Research And Development Co. Ltd. At The Weizmann Institute Of Science
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Publication of HRP20231499T1 publication Critical patent/HRP20231499T1/hr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5026—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on cell morphology
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • A—HUMAN NECESSITIES
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/13—Amines
    • A61K31/135—Amines having aromatic rings, e.g. ketamine, nortriptyline
    • A61K31/138—Aryloxyalkylamines, e.g. propranolol, tamoxifen, phenoxybenzamine
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    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/337—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having four-membered rings, e.g. taxol
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/439—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom the ring forming part of a bridged ring system, e.g. quinuclidine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
    • A61K31/445—Non condensed piperidines, e.g. piperocaine
    • A61K31/4523—Non condensed piperidines, e.g. piperocaine containing further heterocyclic ring systems
    • A61K31/4545—Non condensed piperidines, e.g. piperocaine containing further heterocyclic ring systems containing a six-membered ring with nitrogen as a ring hetero atom, e.g. pipamperone, anabasine
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    • A61K31/00—Medicinal preparations containing organic active ingredients
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    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/47—Quinolines; Isoquinolines
    • A61K31/4748—Quinolines; Isoquinolines forming part of bridged ring systems
    • A—HUMAN NECESSITIES
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    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/50—Pyridazines; Hydrogenated pyridazines
    • A61K31/502—Pyridazines; Hydrogenated pyridazines ortho- or peri-condensed with carbocyclic ring systems, e.g. cinnoline, phthalazine
    • A—HUMAN NECESSITIES
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    • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
    • A61K31/513—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim having oxo groups directly attached to the heterocyclic ring, e.g. cytosine
    • A—HUMAN NECESSITIES
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    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
    • A61K31/519—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim ortho- or peri-condensed with heterocyclic rings
    • A—HUMAN NECESSITIES
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/535—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with at least one nitrogen and one oxygen as the ring hetero atoms, e.g. 1,2-oxazines
    • A61K31/5375—1,4-Oxazines, e.g. morpholine
    • A61K31/5377—1,4-Oxazines, e.g. morpholine not condensed and containing further heterocyclic rings, e.g. timolol
    • A—HUMAN NECESSITIES
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    • A61K31/66—Phosphorus compounds
    • A61K31/675—Phosphorus compounds having nitrogen as a ring hetero atom, e.g. pyridoxal phosphate
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    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7028—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages
    • A61K31/7034—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin
    • A61K31/704—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin attached to a condensed carbocyclic ring system, e.g. sennosides, thiocolchicosides, escin, daunorubicin
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    • A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
    • A61K31/7052—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides
    • A61K31/706—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom
    • A61K31/7064—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines
    • A61K31/7068—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines having oxo groups directly attached to the pyrimidine ring, e.g. cytidine, cytidylic acid
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Claims (15)

1. Sustav kulture koji se sastoji od medija kulture i precizno izrezanog režnja tkiva postavljenog na umetak kulture tkiva, pri čemu se navedeni precizno izrezani režanj tkiva održava u visoko oksigeniranoj atmosferi koja sadrži najmanje 60 % i manje od 95 % kisika i pri čemu se navedena kultura rotacijski trese olakšavajući povremeno uranjanje navedenog režnja tkiva u navedeni medij kulture.
2. Sustav kulture prema patentnom zahtjevu 1, pri čemu je navedena kultura u ploči s kulturom tkiva.
3. Sustav kulture prema bilo kojem od patentnih zahtjeva 1-2, koji sadrži lijek.
4. Metoda kultiviranja tkiva, metoda koja uključuje kultiviranje precizno izrezanog režnja tkiva na umetku za kulturu tkiva u mediju kulture u visoko oksigeniranij atmosferi koja sadrži najmanje 60 % i manje od 95 % kisika; i trešnja kulture u rotaciji olakšavajući povremeno uranjanje navedenog režnja tkiva u navedeni medij kulture.
5. Metoda prema patentnom zahtjevu 4, koja nadalje sadrži dodavanje lijeka navedenom mediju kulture.
6. Metoda određivanja učinkovitosti lijeka ili serije lijeka, metoda koja obuhvaća: (i) kulturu tkiva u skladu s metodom prema patentnom zahtjevu 4; (i) dodavanje lijeka ili serije lijeka navedenom mediju kulture; i (iii) određivanje učinka navedenog lijeka ili navedene serije lijeka na navedeno tkivo, pri čemu osjetljivost navedenog tkiva navedenom lijeku ukazuje na učinkovitost navedenog lijeka.
7. Sustav kulture prema bilo kojem od patentnih zahtjeva 1-3 ili metoda prema bilo kojem od patentnih zahtjeva 4-6, pri čemu je navedeno tkivo patološko tkivo.
8. Metoda odabira lijeka za liječenje bolesti kod subjekta kojem je to potrebno, metoda koja obuhvaća: (i) kulturu patološkog tkiva dobivenog od subjekta prema metodi prema patentnom zahtjevu 4; (ii) dodavanje lijeka navedenom mediju kulture; i (iii) određivanje učinka navedenog lijeka na navedeno tkivo, pri čemu osjetljivost navedenog tkiva na navedeni lijek ukazuje učinkovitost navedenog lijeka za liječenje navedene bolesti kod navedenog subjekta.
9. Sustav kulture ili metoda prema bilo kojem od patentnih zahtjeva 7-8, pri čemu je navedeno patološko tkivo kancerogeno tkivo.
10. Sustav kulture ili metoda prema bilo kojem od patentnih zahtjeva 1-8, pri čemu je navedeno tkivo odabrano iz skupine koja se sastoji od jajnika, debelog crijeva, pluća, gušterače, želuca, gastroezofagusa, dojke, jetre, hrskavice i kostiju.
11. Sustav kulture ili metoda prema bilo kojem od patentnih zahtjeva 3 i 5-10, pri čemu je navedeni lijek odabran iz skupine koja se sastoji od 5-Flurouricila (5FU), Oksaliplatina, Irinotekana, Cisplatina, 4-hidroperoksi ciklofosfamida, Docetaksela, Doksorubicina, Navalbina, Gemcitabina, Gefitiniba, Tamoksifena, Olapariba, Trametiniba, Everolimusa i Palbocikliba.
12. Metoda prema bilo kojem od patentnih zahtjeva 4-11, pri čemu se navedena kultura provodi najmanje 4 dana.
13. Sustav kulture ili metoda prema bilo kojem od patentnih zahtjeva 1-12, pri čemu je navedeni režanj u izravnom kontaktu s navedenim umetkom kulture tkiva.
14. Sustav kulture ili metoda prema bilo kojem od patentnih zahtjeva 1-13, pri čemu je navedeni umetak kulture tkiva umetak od titanske rešetke.
15. Sustav kulture ili metoda prema bilo kojem od patentnih zahtjeva 1-14, pri čemu navedena visoko oksigenirana atmosfera sadrži najmanje 70 % kisika.
HRP20231499TT 2017-04-05 2018-04-03 Ex-vivo sustav kulture i metode korištenja istog HRP20231499T1 (hr)

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