HRP20210696T1 - Poboljšana sredstva za pročišćavanje vode - Google Patents
Poboljšana sredstva za pročišćavanje vode Download PDFInfo
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- HRP20210696T1 HRP20210696T1 HRP20210696TT HRP20210696T HRP20210696T1 HR P20210696 T1 HRP20210696 T1 HR P20210696T1 HR P20210696T T HRP20210696T T HR P20210696TT HR P20210696 T HRP20210696 T HR P20210696T HR P20210696 T1 HRP20210696 T1 HR P20210696T1
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- bacterial cells
- expression vector
- tyr1
- seq
- tyrosinase
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims 5
- 238000000746 purification Methods 0.000 title 1
- 230000001580 bacterial effect Effects 0.000 claims 20
- 239000013604 expression vector Substances 0.000 claims 11
- 239000000126 substance Substances 0.000 claims 10
- 238000000034 method Methods 0.000 claims 9
- 101100315624 Caenorhabditis elegans tyr-1 gene Proteins 0.000 claims 8
- 102000003425 Tyrosinase Human genes 0.000 claims 8
- 108060008724 Tyrosinase Proteins 0.000 claims 8
- 239000000203 mixture Substances 0.000 claims 6
- 239000007787 solid Substances 0.000 claims 4
- 241000588724 Escherichia coli Species 0.000 claims 3
- 230000006698 induction Effects 0.000 claims 3
- 102000004169 proteins and genes Human genes 0.000 claims 3
- 108090000623 proteins and genes Proteins 0.000 claims 3
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 2
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 claims 2
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 claims 2
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 2
- 238000004113 cell culture Methods 0.000 claims 2
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 claims 2
- 150000007523 nucleic acids Chemical group 0.000 claims 2
- 239000000243 solution Substances 0.000 claims 2
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 claims 2
- 239000002351 wastewater Substances 0.000 claims 2
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 claims 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims 1
- 230000002378 acidificating effect Effects 0.000 claims 1
- 150000001413 amino acids Chemical group 0.000 claims 1
- 239000003637 basic solution Substances 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 239000007857 degradation product Substances 0.000 claims 1
- 239000003814 drug Substances 0.000 claims 1
- 229910001385 heavy metal Inorganic materials 0.000 claims 1
- 108010037896 heparin-binding hemagglutinin Proteins 0.000 claims 1
- 230000001939 inductive effect Effects 0.000 claims 1
- 239000008101 lactose Substances 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 239000012528 membrane Substances 0.000 claims 1
- 150000002736 metal compounds Chemical class 0.000 claims 1
- 239000000843 powder Substances 0.000 claims 1
- 238000002360 preparation method Methods 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/16—Enzymes or microbial cells immobilised on or in a biological cell
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0055—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
- C12N9/0057—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
- C12N9/0059—Catechol oxidase (1.10.3.1), i.e. tyrosinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y110/00—Oxidoreductases acting on diphenols and related substances as donors (1.10)
- C12Y110/03—Oxidoreductases acting on diphenols and related substances as donors (1.10) with an oxygen as acceptor (1.10.3)
- C12Y110/03001—Catechol oxidase (1.10.3.1), i.e. tyrosinase
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/34—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32
- C02F2103/343—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32 from the pharmaceutical industry, e.g. containing antibiotics
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
- C02F3/08—Aerobic processes using moving contact bodies
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
- C02F3/12—Activated sludge processes
- C02F3/1236—Particular type of activated sludge installations
- C02F3/1268—Membrane bioreactor systems
- C02F3/1273—Submerged membrane bioreactors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Environmental & Geological Engineering (AREA)
- Hydrology & Water Resources (AREA)
- Water Supply & Treatment (AREA)
- Medicinal Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Water Treatment By Sorption (AREA)
Claims (18)
1. Ekspresijski vektor za ekspresiju tirozinaze (Tyr1) koji treba biti izložen na površini bakterijske stanice, pri čemu navedeni ekspresijski vektor sadrži ekspresijsku kasetu koja kodira tirozinazu (Tyr1) kao putnika u sustavu beta-autotransportera tipa V, pri čemu navedena tirozinaza (Tyr1) sadrži aminokiselinski slijed SEQ ID NO: 1 kada se eksprimira.
2. Ekspresijski vektor prema zahtjevu 1, pri čemu je navedena tirozinaza (Tyr1) kodirana slijedom nukleinske kiseline koji sadrži SEQ ID NO: 2 ili slijedom koji ima barem 80 %, kao što je 85, 90 ili 95 % jednakosti sa SEQ ID NO: 2.
3. Ekspresijski vektor prema bilo kojem prethodnom zahtjevu, pri čemu navedena ekspresijska kaseta kodira protein koji je tirozinaza (Tyr1) kao putnik u sustavu beta-autotransportera AIDA (engl. “Adhesin Involved in Diffuse Adherence”, adhezin uključen u difuzno pridržavanje) tipa V, kao što je sustav beta-autotransportera AIDA-I tipa V (SEQ ID NO: 3) ili kodira protein koji ima barem 95 % jednakosti sa SEQ ID NO: 3.
4. Ekspresijski vektor prema zahtjevu 3, pri čemu je protein kodiran slijedom nukleinske kiseline koji sadrži SEQ ID NO: 5 ili slijedom koji ima barem 80 % jednakosti, kao što je 85 %, 90 % ili 95 % jednakosti sa SEQ ID NO: 5.
5. Ekspresijski vektor prema bilo kojem od zahtjeva 1 – 4, pri čemu je navedeni ekspresijski vektor pAIDA1-Tyr1 (SEQ ID NO: 4) ili ekspresijski vektor koji ima barem 80 % jednakosti slijeda s istim, kao što je 85 %, 90 % ili 95 % jednakosti sa slijedom SEQ ID NO: 4.
6. Stanica domaćina koja sadrži ekspresijski vektor prema bilo kojem od zahtjeva 1 – 5, poput stanice E.coli, kao što je soj E.coli K12, najbolje E.coli 0:17K12∆OmpT.
7. Postupak za proizvodnju jedne ili više bakterijskih stanica, barem djelomično presvučenih sastavom koji adsorbira kemijske tvari, a da navedeni postupak sadrži sljedeće korake:
a) odabir i uzgoj jedne ili više bakterijskih stanica koje sadrže ekspresijski vektor prema bilo kojem od zahtjeva 1 – 5 ili stanicu domaćina prema zahtjevu 6.
b) induciranje ekspresije tirozinaze (Tyr1) iz navedenog ekspresijskog vektora kako bi se omogućilo izlaganje navedene tirozinaze (Tyr1) na površini navedene jedne ili više bakterijskih stanica,
c) dodavanje odgovarajuće količine tirozina ili njegovog funkcionalnog ekvivalenta navedenoj jednoj ili više bakterijskih stanica kako bi se omogućilo stvaranje navedenog sastava koji adsorbira kemijske tvari barem djelomično na površini navedene jedne ili više bakterijskih stanica, i nakon toga
d) pribavljanje stanica iz koraka c).
8. Postupak prema zahtjevu 7, pri čemu se uzgoj stanica izvodi pri pH od oko 7 – 8, kao što je pri približno pH 7,5, a po izboru pri čemu se induciranje u koraku b) izvodi dodavanjem IPTG-a ili laktoze u kulturu bakterijskih stanica, na primjer u količini od oko 200 µM IPTG-a po indukciji.
9. Postupak prema bilo kojem od zahtjeva 7 – 8, pri čemu se ekspresija tirozinaze (Tyr1) može nastaviti tijekom barem dvije generacije stanica nakon induciranja u koraku b), kao na primjer 2, 3, 4, 5, ili 6 generacija.
10. Postupak prema bilo kojem od zahtjeva 7 – 9, pri čemu je tirozin u obliku praškaste smjese ili je sadržan u vodenoj bazičnoj otopini koja ima pH oko 9 – 12, na primjer otprilike pH 10.
11. Postupak prema bilo kojem od zahtjeva 7 – 10, pri čemu sastav koji adsorbira kemijske tvari sadrži melanin i/ili njegov funkcionalni ekvivalent.
12. Postupak prema bilo kojem od zahtjeva 7 – 11, pri čemu kemijska tvar sadrži farmaceutsku tvar ili jedan ili više proizvoda razgradnje ili jedan ili više spojeva teških metala.
13. Jedna ili više bakterijskih stanica, barem djelomično presvučenih sastavom koji adsorbira kemijske tvari, a koji se može dobiti postupkom prema bilo kojem od zahtjeva 7 – 12.
14. Upotreba jedne ili više bakterijskih stanica, barem djelomično presvučenih sastavom koji adsorbira kemijske tvari prema zahtjevu 13, za uklanjanje jedne ili više kemijskih tvari iz vode, kao što je otpadna voda.
15. Upotreba prema zahtjevu 14, pri čemu su navedena jedna ili više bakterijskih stanica suspendirane u otopini ili prisutne u biofilmu, po izboru gdje se navedeni biofilm nalazi na čvrstom nosaču, kao što je stacionarni čvrsti nosač ili pokretni čvrsti nosač.
16. Uređaj, kao što je MBR (engl. “Membrane Bioreactor”, membranski bioreaktor), koji sadrži jednu ili više bakterijskih stanica prema zahtjevu 13, suspendirane u otopini.
17. Uređaj, kao što je MBBR (engl. “Moving bed biofilm reactor”, reaktor s biofilmom s pomičnim slojem), koji sadrži jednu ili više bakterijskih stanica prema zahtjevu 13, koje se nalaze u biofilmu na čvrstom nosaču.
18. Postupak za uklanjanje jedne ili više kemijskih tvari iz vode, poput otpadnih voda, koji sadrži korake:
a) Osiguravanje jedne ili više bakterijskih stanica prema zahtjevu 13, ili biofilm koji sadrži navedenu jednu ili više bakterijskih stanica,
b) Omogućavanje prolaska vode kroz jednu ili više bakterijskih stanica ili navedeni biofilm koji sadrži navedenu jednu ili više bakterijskih stanica, adsorbirajući pritom sve kemijske tvari prisutne u toj vodi,
c) Uklanjanje svih adsorbiranih kemijskih tvari iz jedne ili više bakterijskih stanica ili biofilma koji sadrži navedenu jednu ili više bakterijskih stanica, ispiranjem vodenim pripravkom kiselog pH, da bi se nakon toga omogućila ponovna upotreba jedne ili više bakterijskih stanica ili biofilma koji sadrži navedenu jednu ili više bakterijskih stanica i nakon toga
d) Po želji ponavljanje koraka b) i c).
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE1530178 | 2015-11-20 | ||
| SE1530180A SE540415C2 (en) | 2015-11-20 | 2015-11-25 | Bacterial cells expressing tyrosinase (Tyr 1) for use in water purification |
| PCT/EP2016/078065 WO2017085217A1 (en) | 2015-11-20 | 2016-11-17 | Improved means for water purification |
| EP16802001.4A EP3377619B1 (en) | 2015-11-20 | 2016-11-17 | Improved means for water purification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20210696T1 true HRP20210696T1 (hr) | 2021-06-11 |
Family
ID=59057947
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20210696TT HRP20210696T1 (hr) | 2015-11-20 | 2021-05-05 | Poboljšana sredstva za pročišćavanje vode |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP3377619B1 (hr) |
| DK (1) | DK3377619T3 (hr) |
| ES (1) | ES2866075T3 (hr) |
| HR (1) | HRP20210696T1 (hr) |
| SE (1) | SE540415C2 (hr) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113373077B (zh) * | 2021-02-07 | 2023-02-07 | 南宁海关技术中心 | 一种氯霉素高效降解菌、高效降解菌剂及其应用 |
-
2015
- 2015-11-25 SE SE1530180A patent/SE540415C2/en unknown
-
2016
- 2016-11-17 EP EP16802001.4A patent/EP3377619B1/en active Active
- 2016-11-17 DK DK16802001.4T patent/DK3377619T3/da active
- 2016-11-17 ES ES16802001T patent/ES2866075T3/es active Active
-
2021
- 2021-05-05 HR HRP20210696TT patent/HRP20210696T1/hr unknown
Also Published As
| Publication number | Publication date |
|---|---|
| ES2866075T3 (es) | 2021-10-19 |
| EP3377619A1 (en) | 2018-09-26 |
| EP3377619B1 (en) | 2021-02-17 |
| SE540415C2 (en) | 2018-09-11 |
| DK3377619T3 (da) | 2021-04-26 |
| SE1530180A1 (en) | 2017-05-21 |
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