HRP20171811T1 - Postupak za kontinuiranu proizvodnju celulaza pomoću filamentoznih gljivica koje koriste karbonatni supstrat dobiven iz predobrade kiselinom - Google Patents
Postupak za kontinuiranu proizvodnju celulaza pomoću filamentoznih gljivica koje koriste karbonatni supstrat dobiven iz predobrade kiselinom Download PDFInfo
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- HRP20171811T1 HRP20171811T1 HRP20171811TT HRP20171811T HRP20171811T1 HR P20171811 T1 HRP20171811 T1 HR P20171811T1 HR P20171811T T HRP20171811T T HR P20171811TT HR P20171811 T HRP20171811 T HR P20171811T HR P20171811 T1 HRP20171811 T1 HR P20171811T1
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- 239000000758 substrate Substances 0.000 title claims 18
- 238000000034 method Methods 0.000 title claims 12
- 102000005575 Cellulases Human genes 0.000 title claims 5
- 108010084185 Cellulases Proteins 0.000 title claims 5
- 239000002253 acid Substances 0.000 title claims 4
- 238000010924 continuous production Methods 0.000 title claims 4
- 241000233866 Fungi Species 0.000 title claims 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title 1
- 229910052799 carbon Inorganic materials 0.000 title 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 claims 12
- 230000001939 inductive effect Effects 0.000 claims 10
- 239000007864 aqueous solution Substances 0.000 claims 5
- 230000012010 growth Effects 0.000 claims 5
- 229920002488 Hemicellulose Polymers 0.000 claims 4
- 238000004519 manufacturing process Methods 0.000 claims 3
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 claims 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims 2
- 230000003698 anagen phase Effects 0.000 claims 2
- 238000006243 chemical reaction Methods 0.000 claims 2
- 239000012895 dilution Substances 0.000 claims 2
- 238000010790 dilution Methods 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 2
- 235000000346 sugar Nutrition 0.000 claims 2
- 150000008163 sugars Chemical class 0.000 claims 2
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 claims 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 1
- 235000010469 Glycine max Nutrition 0.000 claims 1
- 244000068988 Glycine max Species 0.000 claims 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims 1
- 241000499912 Trichoderma reesei Species 0.000 claims 1
- 238000005903 acid hydrolysis reaction Methods 0.000 claims 1
- 238000010306 acid treatment Methods 0.000 claims 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims 1
- 238000010411 cooking Methods 0.000 claims 1
- 230000002255 enzymatic effect Effects 0.000 claims 1
- 238000004880 explosion Methods 0.000 claims 1
- 239000000284 extract Substances 0.000 claims 1
- 238000000855 fermentation Methods 0.000 claims 1
- 230000004151 fermentation Effects 0.000 claims 1
- 239000008103 glucose Substances 0.000 claims 1
- 108010002430 hemicellulase Proteins 0.000 claims 1
- 238000005470 impregnation Methods 0.000 claims 1
- 239000008101 lactose Substances 0.000 claims 1
- 239000000178 monomer Substances 0.000 claims 1
- 230000035772 mutation Effects 0.000 claims 1
- 230000006798 recombination Effects 0.000 claims 1
- 230000001954 sterilising effect Effects 0.000 claims 1
- 238000004659 sterilization and disinfection Methods 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/01—Carboxylic ester hydrolases (3.1.1)
- C12Y301/01073—Feruloyl esterase (3.1.1.73)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Botany (AREA)
- Mycology (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Chemical Or Physical Treatment Of Fibers (AREA)
Claims (11)
1. Postupak za proizvodnju celulaza i hemicelulaza upotrebom soja filamentoznih gljivica u miješanom i aeriranom bioreaktoru, koji sadrži barem dvije faze:
• fazu a) rasta navedenog soja u prisutnosti barem jednog karbonatnog supstrata za rast u zatvorenom reaktoru, navedena faza rasta se provodi s koncentracijom karbonatnog supstrata za rast u rasponu 10 do 90 g/L;
• fazu b) za kontinuiranu proizvodnju celulaza, u kojoj se barem jedan karbonatni inducirajući supstrat dovodi uz brzinu dovođenja koja je konstantna tijekom perioda od barem više od 200 h, navedeni karbonatni inducirajući supstrat je barem jedna vodena otopina hemiceluloznog hidrolizata dobivena iz prethodne obrade s kiselinom lignoceluloznog supstrata, navedena vodena otopina hemiceluloznog hidrolizata nije podvrgnuta prethodnoj sterilizaciji i nije podvrgnuta ispravljanju pH, navedeni pH vodene otopine je u rasponu 0.5 do 3, masa reakcijskog volumena se održava konstantnom povlačenjem djela navedenog reakcijskog volumena, navedena faza b) se provodi pri brzini razrjeđivanja u rasponu 0.001 do 0.008 h-1,
u kojem je karbonatni inducirajući supstrat korišten u fazi b) vodena otopina hemiceluloznog hidrolizata dobivena iz prethodne obrade s kiselinom lignoceluloznog supstrata, kao smjesa s barem jednim drugim karbonatnim supstratom odabranim od inducirajućih ili ne inducirajućih šećera, u kojem je brzina dovođenja navedenog karbonatnog inducirajućeg supstrata u rasponu 35 do 140 mg karbonatnog inducirajućeg supstrata po gramu mase suhe tvari soja na sat.
2. Postupak prema zahtjevu 1, naznačen time da navedeni soj je soj Trichoderma reesei modificiran pomoću mutacije, selekcije ili genetske rekombinacije.
3. Postupak prema zahtjevu 1 ili zahtjevu 2, naznačen time da je karbonatna podloga za rast koja se koristi u navedenoj fazi rasta odabrana od ostataka glukoze, laktoze, ksiloze, dobivenih nakon etanolne fermentacije monomernih šećera enzimskih hidrolizata lignoceluloznog supstrata i ekstrakata hemicelulozne frakcije u obliku monomera dobivenih od prethodno obrađenog lignoceluloznog supstrata, koristi samostalno ili kao smjesa.
4. Postupak prema jednom od zahtjeva 1 do 3, naznačen time da kisela predobrada je kisela hidroliza, kuhanje s kiselinom ili eksplozija pare s prethodnom impregnacijom navedenog lignoceluloznog supstrata s vodenom otopinom sumporne kiseline.
5. Postupak prema jednom od zahtjeva 1 do 4, naznačen time da je trajanje faze b) za neprekinutu proizvodnju celulaza veće od barem 300 h.
6. Postupak prema zahtjevu 5, naznačen time da je trajanje faze b) za neprekinutu proizvodnju celulaza veće od barem 400 h.
7. Postupak prema jednom od zahtjeva 1 do 6, naznačen time da se brzina protoka dovoda postupno povećava u fazi b).
8. Postupak prema jednom od zahtjeva 1 do 7, naznačen time da se navedena faza b) provodi pri brzini razrjeđivanja u rasponu 0.002 do 0.008 h-1.
9. Postupak prema jednom od zahtjeva 1 do 8, naznačen time da se proizvoljna faza a') rasta i proizvodnje u reaktoru s kontinuiranom dobavom barem jednog karbonatnog inducirajućeg supstrata tijekom koje nema kontinuiranog povlačenja iz fermentora, provodi između faze a) i faze b).
10. Postupak prema zahtjevu 9, naznačen time da je navedeni karbonatni inducirajući supstrat koji je korišten u fazi a') identičan karbonatnom inducirajućem supstratu korištenom u fazi proizvodnje b).
11. Postupak prema zahtjevu 9 ili zahtjevu 10, naznačen time da se faza a') provodi kroz razdoblje u rasponu od 50 do 150 h.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR1103149A FR2981364B1 (fr) | 2011-10-14 | 2011-10-14 | Procede de production de cellulases en continu par un champignon filamenteux utilisant un substrat carbone issu d'un pretraitement acide |
| PCT/FR2012/000381 WO2013054005A1 (fr) | 2011-10-14 | 2012-09-26 | Procede de production de cellulases en continu par un champignon filamenteux utilisant un substrat carbone issu d'un pretraitement acide |
| EP12775738.3A EP2766471B1 (fr) | 2011-10-14 | 2012-09-26 | Procede de production de cellulases en continu par un champignon filamenteux utilisant un substrat carbone issu d'un pretraitement acide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20171811T1 true HRP20171811T1 (hr) | 2017-12-29 |
Family
ID=47071324
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20171811TT HRP20171811T1 (hr) | 2011-10-14 | 2017-11-21 | Postupak za kontinuiranu proizvodnju celulaza pomoću filamentoznih gljivica koje koriste karbonatni supstrat dobiven iz predobrade kiselinom |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US9249402B2 (hr) |
| EP (1) | EP2766471B1 (hr) |
| CN (1) | CN104024399B (hr) |
| AU (1) | AU2012322594B2 (hr) |
| BR (1) | BR112014008847B1 (hr) |
| CA (1) | CA2850609C (hr) |
| DK (1) | DK2766471T3 (hr) |
| ES (1) | ES2641254T3 (hr) |
| FR (1) | FR2981364B1 (hr) |
| HR (1) | HRP20171811T1 (hr) |
| HU (1) | HUE037331T2 (hr) |
| IN (1) | IN2014CN03513A (hr) |
| NO (1) | NO2766471T3 (hr) |
| PL (1) | PL2766471T3 (hr) |
| PT (1) | PT2766471T (hr) |
| WO (1) | WO2013054005A1 (hr) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2991997B1 (fr) | 2012-06-18 | 2015-08-21 | IFP Energies Nouvelles | Procede de production d'un cocktail enzymatique utilisant les residus liquides d'un procede de conversion biochimique de materiaux ligno-cellulosiques |
| FR3049957B1 (fr) * | 2016-04-08 | 2020-09-25 | Ifp Energies Now | Procede de production de cellulases avec du marc lignocellulosique pretraite |
| FR3085961B1 (fr) * | 2018-09-14 | 2024-05-10 | Ifp Energies Now | Procede de production de cellulases par un champignon filamenteux |
| FR3088934A1 (fr) * | 2018-11-26 | 2020-05-29 | IFP Energies Nouvelles | Procede de production d’enzymes par une souche appartenant a un champignon filamenteux |
| US11242549B2 (en) | 2019-04-17 | 2022-02-08 | Indian Oil Corporation Limited | Bio-refinery waste utilization for enzyme production using novel penicillium funiculosum MRJ-16 fungal strain |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4275167A (en) | 1980-06-18 | 1981-06-23 | The United States Of America As Represented By The Secretary Of Agriculture | Preferential degradation of lignin in gramineous materials |
| CN1036792A (zh) * | 1988-09-23 | 1989-11-01 | 山东大学 | 高活性纤维素酶的制造方法 |
| CN1063895A (zh) * | 1991-02-04 | 1992-08-26 | 无锡轻工业学院 | 纤维素酶生产及其应用技术 |
| FR2881753B1 (fr) | 2005-02-09 | 2009-10-02 | Inst Francais Du Petrole | Procede de production d'enzymes cellulolytiques et hemicellulolytiques utilisant les residus de distillation de fermentation ethanolique d'hydrolysats enzymatiques de materiaux (ligno-)cellulosique |
| MX2010002185A (es) * | 2007-08-30 | 2010-06-17 | Iogen Energy Corp | Metodo para produccion de celulasa. |
| FR2962444B1 (fr) | 2010-07-12 | 2012-08-31 | Inst Francais Du Petrole | Procede de production d'enzymes cellulolytiques et/ou hemicellulolytiques ameliore |
-
2011
- 2011-10-14 FR FR1103149A patent/FR2981364B1/fr active Active
-
2012
- 2012-09-26 ES ES12775738.3T patent/ES2641254T3/es active Active
- 2012-09-26 US US14/350,827 patent/US9249402B2/en active Active
- 2012-09-26 CA CA2850609A patent/CA2850609C/fr active Active
- 2012-09-26 CN CN201280050395.5A patent/CN104024399B/zh active Active
- 2012-09-26 BR BR112014008847-0A patent/BR112014008847B1/pt active IP Right Grant
- 2012-09-26 HU HUE12775738A patent/HUE037331T2/hu unknown
- 2012-09-26 WO PCT/FR2012/000381 patent/WO2013054005A1/fr active Application Filing
- 2012-09-26 PT PT127757383T patent/PT2766471T/pt unknown
- 2012-09-26 PL PL12775738T patent/PL2766471T3/pl unknown
- 2012-09-26 NO NO12775738A patent/NO2766471T3/no unknown
- 2012-09-26 AU AU2012322594A patent/AU2012322594B2/en active Active
- 2012-09-26 IN IN3513CHN2014 patent/IN2014CN03513A/en unknown
- 2012-09-26 DK DK12775738.3T patent/DK2766471T3/da active
- 2012-09-26 EP EP12775738.3A patent/EP2766471B1/fr active Active
-
2017
- 2017-11-21 HR HRP20171811TT patent/HRP20171811T1/hr unknown
Also Published As
| Publication number | Publication date |
|---|---|
| FR2981364A1 (fr) | 2013-04-19 |
| AU2012322594A1 (en) | 2014-04-24 |
| DK2766471T3 (da) | 2017-11-27 |
| WO2013054005A1 (fr) | 2013-04-18 |
| PL2766471T3 (pl) | 2018-02-28 |
| CA2850609C (fr) | 2019-12-31 |
| HUE037331T2 (hu) | 2018-08-28 |
| BR112014008847A2 (pt) | 2017-04-25 |
| CN104024399B (zh) | 2016-10-19 |
| FR2981364B1 (fr) | 2015-01-30 |
| AU2012322594B2 (en) | 2017-12-14 |
| EP2766471B1 (fr) | 2017-08-23 |
| CN104024399A (zh) | 2014-09-03 |
| IN2014CN03513A (hr) | 2015-10-09 |
| EP2766471A1 (fr) | 2014-08-20 |
| BR112014008847B1 (pt) | 2020-11-03 |
| CA2850609A1 (fr) | 2013-04-18 |
| ES2641254T3 (es) | 2017-11-08 |
| US20140295524A1 (en) | 2014-10-02 |
| NO2766471T3 (hr) | 2018-01-20 |
| PT2766471T (pt) | 2017-10-03 |
| US9249402B2 (en) | 2016-02-02 |
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