Claims (14)
1. Farmaceutski pripravak namijenjen poticanju imunosnog odgovora na tumorske stanice, naznačen time što sadrži
a) tumorske stanice koje postaju apoptotske zbog obrade visokim hidrostatskim tlakom jednakim ili većim od 100 MPa u trajanju od 10 minuta do 2 sata i
b) dendritičke stanice.1. Pharmaceutical preparation intended to stimulate the immune response to tumor cells, indicated by the fact that it contains
a) tumor cells that become apoptotic due to treatment with high hydrostatic pressure equal to or greater than 100 MPa for a duration of 10 minutes to 2 hours and
b) dendritic cells.
2. Farmaceutski pripravak u skladu s patentnim zahtjevom 1, naznačen time što se tumorske stanice obrađuje hidrostatskim tlakom najmanje 10 minuta pod tlakom od najmanje 200 MPa.2. Pharmaceutical preparation according to patent claim 1, characterized in that the tumor cells are treated with hydrostatic pressure for at least 10 minutes under a pressure of at least 200 MPa.
3. Farmaceutski pripravak u skladu s patentnim zahtjevom 1, naznačen time što se tumorske stanice obrađuje hidrostatskim tlakom od 200-300 MPa.3. Pharmaceutical preparation according to claim 1, characterized in that the tumor cells are treated with a hydrostatic pressure of 200-300 MPa.
4. Farmaceutski pripravak u skladu s bilo kojim od patentnih zahtjeva 1-3, naznačen time što apoptotske tumorske stanice nisu nekrotične.4. Pharmaceutical preparation according to any one of claims 1-3, characterized in that the apoptotic tumor cells are not necrotic.
5. Farmaceutski pripravak u skladu s bilo kojim od prethodnih patentnih zahtjeva, naznačen time što se tumorske stanice dobiva iz tumorskih staničnih linija.5. Pharmaceutical preparation according to any of the previous patent claims, characterized in that the tumor cells are obtained from tumor cell lines.
6. Farmaceutski pripravak u skladu s bilo kojim od patentnih zahtjeva 1-4, naznačen time što se tumorske stanice dobiva iz tumora izoliranog iz pacijenta kojeg treba liječiti.6. A pharmaceutical preparation according to any one of claims 1-4, characterized in that the tumor cells are obtained from a tumor isolated from a patient to be treated.
7. Farmaceutski pripravak u skladu s bilo kojim od patentnih zahtjeva 1-6, naznačen time što su nezrele dendritičke stanice opterećene s apoptotskim tumorskim stanicama obrađenim visokim hidrostatskim tlakom.7. Pharmaceutical preparation according to any one of patent claims 1-6, characterized in that immature dendritic cells are loaded with apoptotic tumor cells treated with high hydrostatic pressure.
8. Farmaceutski pripravak u skladu s patentnim zahtjevom 7, naznačen time što se nezrele dendritičke stanice dobivaju leukoferezom.8. Pharmaceutical preparation according to patent claim 7, characterized in that immature dendritic cells are obtained by leukopheresis.
9. Farmaceutski pripravak u skladu s patentnim zahtjevom 8, naznačen time što se nezrele dendritičke stanice dobivaju leukoferezom i uzgojem in vitro u prisutnosti citokina.9. Pharmaceutical preparation according to patent claim 8, characterized in that immature dendritic cells are obtained by leukopheresis and cultivation in vitro in the presence of cytokines.
10. Farmaceutski pripravak u skladu s bilo kojim od patentnih zahtjeva 1 do 9, naznačen time što je namijenjen upotrebi kod pacijenata oboljelih od ranog kao i kasnog stadija raka.10. Pharmaceutical preparation according to any one of patent claims 1 to 9, characterized in that it is intended for use in patients suffering from early as well as late stage cancer.
11. Farmaceutski pripravak u skladu s bilo kojim od patentnih zahtjeva 1 do 9, naznačen time što je namijenjen upotrebi u liječenju raka, kojeg čine rak prostate, rak jajnika ili akutna limfoblastična leukemija.11. Pharmaceutical preparation according to any one of patent claims 1 to 9, characterized in that it is intended for use in the treatment of cancer, which consists of prostate cancer, ovarian cancer or acute lymphoblastic leukemia.
12. Postupak priprave farmaceutskog pripravka u skladu s bilo kojim od patentnih zahtjeva 1-9, naznačen time što se nezrele dendritičke stanice stavlja u kontakt s apoptotskim tumorskim stanicama obrađenim visokim hidrostatskim tlakom jednakim ili većim od 100 MPa i naknadno zrenje dendritičkih stanica.12. Process for the preparation of a pharmaceutical preparation according to any one of patent claims 1-9, characterized in that immature dendritic cells are brought into contact with apoptotic tumor cells treated with high hydrostatic pressure equal to or greater than 100 MPa and subsequent maturation of dendritic cells.
13. Postupak priprave farmaceutskog pripravka u skladu s patentnim zahtjevom 12, naznačen time što je namijenjen upotrebi u cijepljenju od ljudskog raka koje se sastoji u stavljanju u kontakt nezrelih dendritičkih stanica s tumorskim stanicama, gdje se navedene tumorske stanice prevodi u apoptotski stadij obradom u trajanju od 10 minuta do 2 sata visokim hidrostatskim tlakom jednakim ili većim od 100 MPa, gdje su dendritičke stanice izborno zrele prije primjene farmaceutskog pripravka.13. The method of preparing a pharmaceutical preparation in accordance with patent claim 12, characterized in that it is intended for use in vaccination against human cancer, which consists in bringing immature dendritic cells into contact with tumor cells, where said tumor cells are translated into the apoptotic stage by processing for a duration of from 10 minutes to 2 hours with high hydrostatic pressure equal to or greater than 100 MPa, where the dendritic cells are optionally matured before the administration of the pharmaceutical preparation.
14. Postupak priprave farmaceutskog pripravka u skladu s patentnim zahtjevom 12, naznačen time što je namijenjen upotrebi u cijepljenju od ljudskog raka koje se sastoji u stavljanju u kontakt nezrelih dendritičkih stanica s tumorskim stanicama dobivenim iz tumorske stanične linije, prevedenim u apoptotski stadij obradom u trajanju od 10 minuta do 2 sata visokim hidrostatskim tlakom jednakim ili većim od 100 MPa, gdje su dendritičke stanice izborno zrele prije primjene farmaceutskog pripravka.14. The method of preparing a pharmaceutical preparation in accordance with patent claim 12, characterized in that it is intended for use in vaccination against human cancer, which consists in bringing immature dendritic cells into contact with tumor cells obtained from a tumor cell line, converted to the apoptotic stage by processing for a duration of from 10 minutes to 2 hours with high hydrostatic pressure equal to or greater than 100 MPa, where the dendritic cells are optionally matured before the administration of the pharmaceutical preparation.