HK1211260A1 - Method and kit for nucleic acid sequencing - Google Patents

Method and kit for nucleic acid sequencing Download PDF

Info

Publication number
HK1211260A1
HK1211260A1 HK15112192.4A HK15112192A HK1211260A1 HK 1211260 A1 HK1211260 A1 HK 1211260A1 HK 15112192 A HK15112192 A HK 15112192A HK 1211260 A1 HK1211260 A1 HK 1211260A1
Authority
HK
Hong Kong
Prior art keywords
kit
nucleic acid
acid sequencing
sequencing
reagents
Prior art date
Application number
HK15112192.4A
Other languages
English (en)
Chinese (zh)
Inventor
Jonathan O'halloran
Christopher Adams
Joe HEDLEY
Sam WHITEHOUSE
Original Assignee
Quantumdx Group Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Quantumdx Group Limited filed Critical Quantumdx Group Limited
Publication of HK1211260A1 publication Critical patent/HK1211260A1/xx

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Nanotechnology (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Food Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Fluid Mechanics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
HK15112192.4A 2012-08-06 2013-08-05 Method and kit for nucleic acid sequencing HK1211260A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261680212P 2012-08-06 2012-08-06
US61/680,212 2012-08-06
PCT/IB2013/002168 WO2014024041A1 (en) 2012-08-06 2013-08-05 Method and kit for nucleic acid sequencing

Publications (1)

Publication Number Publication Date
HK1211260A1 true HK1211260A1 (en) 2016-05-20

Family

ID=49354708

Family Applications (1)

Application Number Title Priority Date Filing Date
HK15112192.4A HK1211260A1 (en) 2012-08-06 2013-08-05 Method and kit for nucleic acid sequencing

Country Status (7)

Country Link
US (1) US20150276709A1 (cs)
EP (1) EP2879793A1 (cs)
JP (1) JP2015528282A (cs)
CN (1) CN104703700B (cs)
HK (1) HK1211260A1 (cs)
IN (1) IN2015DN01300A (cs)
WO (1) WO2014024041A1 (cs)

Families Citing this family (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10759824B2 (en) 2008-09-03 2020-09-01 Quantumdx Group Limited Design, synthesis and use of synthetic nucleotides comprising charge mass tags
JP5818683B2 (ja) 2008-09-03 2015-11-18 クワンタムディーエックス・グループ・リミテッド 核酸配列決定のための方法およびキット
US9708656B2 (en) 2013-05-06 2017-07-18 Pacific Biosciences Of California, Inc. Real-time electronic sequencing
US20160054312A1 (en) 2014-04-28 2016-02-25 Nanomedical Diagnostics, Inc. Chemically differentiated sensor array
US11215580B2 (en) 2014-04-28 2022-01-04 Cardea Bio, Inc. System and method for DNA sequencing and blood chemistry analysis
US9618476B2 (en) * 2014-04-28 2017-04-11 Nanomedical Diagnostics, Inc. System and method for electronic biological sample analysis
US9765395B2 (en) 2014-04-28 2017-09-19 Nanomedical Diagnostics, Inc. System and method for DNA sequencing and blood chemistry analysis
CN105624020B (zh) * 2014-11-07 2017-11-03 深圳华大基因研究院 用于检测dna片段的碱基序列的微流控芯片
US9618474B2 (en) 2014-12-18 2017-04-11 Edico Genome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
EP3235010A4 (en) 2014-12-18 2018-08-29 Agilome, Inc. Chemically-sensitive field effect transistor
US10006910B2 (en) 2014-12-18 2018-06-26 Agilome, Inc. Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same
US9859394B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US11921112B2 (en) 2014-12-18 2024-03-05 Paragraf Usa Inc. Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same
US12298301B2 (en) 2014-12-18 2025-05-13 Cardea Bio, Inc. Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same
US10020300B2 (en) 2014-12-18 2018-07-10 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US11782057B2 (en) 2014-12-18 2023-10-10 Cardea Bio, Inc. Ic with graphene fet sensor array patterned in layers above circuitry formed in a silicon based cmos wafer
US9857328B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same
HK1245348A1 (zh) * 2015-02-02 2018-08-24 奥特拉公司 来自样品背景的靶多核苷酸的纳米孔隙检测
CN104778964A (zh) * 2015-04-03 2015-07-15 程志 一种用于疾病诊断的检测u盘
KR101958053B1 (ko) * 2015-06-08 2019-03-13 고려대학교 산학협력단 원자 또는 이온을 선택적으로 투과시키는 필터용 나노 채널 구조물 및 이의 제조 방법
CA3021580A1 (en) * 2015-06-25 2016-12-29 Barry L. Merriman Biomolecular sensors and methods
WO2017024017A1 (en) 2015-08-06 2017-02-09 Pacific Biosciences Of California, Inc. Systems and methods for selectively addressing sparsely arranged electronic measurement devices
EP3400444A4 (en) 2016-01-04 2019-10-16 QuantuMDx Group Limited DESIGN, SYNTHESIS AND USE OF SYNTHETIC NUCLEOTIDES WITH LOADING MASS MARKINGS
US10712334B2 (en) 2016-01-28 2020-07-14 Roswell Biotechnologies, Inc. Massively parallel DNA sequencing apparatus
WO2017132586A1 (en) 2016-01-28 2017-08-03 Roswell Biotechnologies, Inc. Methods and apparatus for measuring analytes using large scale molecular electronics sensor arrays
CN106191230A (zh) * 2016-02-02 2016-12-07 杭州格磊思沃科技有限公司 用氨基酸结合脱氧核苷酸进行dna测序的方法
KR102734671B1 (ko) 2016-02-09 2024-11-25 로스웰 엠이 아이엔씨. 전자 비표지 dna 및 게놈 시퀀싱
US10597767B2 (en) 2016-02-22 2020-03-24 Roswell Biotechnologies, Inc. Nanoparticle fabrication
CN109661274A (zh) * 2016-04-04 2019-04-19 卡尤迪生物科技（北京）有限公司 用于加热生物样品的系统和方法
US10811539B2 (en) 2016-05-16 2020-10-20 Nanomedical Diagnostics, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US9829456B1 (en) 2016-07-26 2017-11-28 Roswell Biotechnologies, Inc. Method of making a multi-electrode structure usable in molecular sensing devices
WO2018132457A1 (en) 2017-01-10 2018-07-19 Roswell Biotechnologies, Inc. Methods and systems for dna data storage
US11656197B2 (en) 2017-01-19 2023-05-23 Roswell ME Inc. Solid state sequencing devices comprising two dimensional layer materials
US10508296B2 (en) 2017-04-25 2019-12-17 Roswell Biotechnologies, Inc. Enzymatic circuits for molecular sensors
CN110546276A (zh) 2017-04-25 2019-12-06 罗斯威尔生命技术公司 用于分子传感器的酶电路
EP3622086A4 (en) 2017-05-09 2021-04-21 Roswell Biotechnologies, Inc LINK PROBE CIRCUITS FOR MOLECULAR SENSORS
US11213825B2 (en) * 2017-05-16 2022-01-04 Wisconsin Alumni Research Foundation System and method for en masse patterning of molecule structures
CN107475072B (zh) * 2017-08-24 2020-12-11 浙江理工大学 基于三维石墨烯界面电极的细胞动态特性监测系统及方法
WO2019046589A1 (en) 2017-08-30 2019-03-07 Roswell Biotechnologies, Inc. PROCESSIVE ENZYME MOLECULAR ELECTRONIC SENSORS FOR STORING DNA DATA
US11100404B2 (en) 2017-10-10 2021-08-24 Roswell Biotechnologies, Inc. Methods, apparatus and systems for amplification-free DNA data storage
US11099136B2 (en) * 2017-12-22 2021-08-24 Regents Of The University Of Minnesota 3D graphene optical sensors and methods of manufacture
DE102018207098B4 (de) 2018-05-08 2024-06-13 Robert Bosch Gmbh Mikrofluidische Vorrichtung und Verfahren zur Nanostruktur-Sequenzierung von Nukleotidsträngen
DE102018207106B4 (de) * 2018-05-08 2024-06-13 Robert Bosch Gmbh Mikrofluidische Sequenzierungsvorrichtung zum Vervielfältigen und Trennen von Molekülketten und Verfahren zum Trennen von aus einer Amplifikationsreaktion gewonnen Molekülketten
US11561197B2 (en) 2018-06-29 2023-01-24 AMMR Joint Venture Electronic detection of a target based on enzymatic cleavage of a reporter moiety
GB201821210D0 (en) * 2018-12-24 2019-02-06 Univ College Dublin Nat Univ Ireland Dublin Charge transfer template
JP2023525890A (ja) * 2020-05-15 2023-06-19 ヘメミクス・バイオテクノロジーズ・インコーポレイテッド 迅速なポイント・オブ・ケア診断のための多重バイオセンサー
WO2023123314A1 (zh) * 2021-12-31 2023-07-06 京东方科技集团股份有限公司 微纳流控基板、芯片、制备方法及系统

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7005264B2 (en) * 2002-05-20 2006-02-28 Intel Corporation Method and apparatus for nucleic acid sequencing and identification
WO2005072089A2 (en) * 2003-12-11 2005-08-11 The Penn State Research Foundation Controlled nanowire in permanent integrated nano-templates and method of fabricating sensor and transducer structures
US20060275779A1 (en) * 2005-06-03 2006-12-07 Zhiyong Li Method and apparatus for molecular analysis using nanowires
CA2964611C (en) * 2007-03-28 2021-06-01 Bionano Genomics, Inc. Methods of macromolecular analysis using nanochannel arrays
EP2014761B1 (en) * 2007-06-22 2016-09-28 Sony Deutschland GmbH A device for processing an analyte and a method of processing and/or detecting an analyte using said device
WO2010002939A2 (en) * 2008-06-30 2010-01-07 Life Technologies Corporation Methods for real time single molecule sequencing
US9605302B2 (en) 2008-09-03 2017-03-28 Quantumdx Group Limited Sensing strategies and methods for nucleic acid detection using biosensors
JP5818683B2 (ja) * 2008-09-03 2015-11-18 クワンタムディーエックス・グループ・リミテッド 核酸配列決定のための方法およびキット
JP5717634B2 (ja) * 2008-09-03 2015-05-13 ナブシス， インコーポレイテッド 流体チャネル内の生体分子および他の分析物の電圧感知のための、長手方向に変位されるナノスケールの電極の使用
US8871921B2 (en) 2008-09-03 2014-10-28 Quantumdx Group Ltd. Design, synthesis and use of synthetic nucleotides comprising charge mass tags
JP5586001B2 (ja) * 2009-08-26 2014-09-10 独立行政法人物質・材料研究機構 ナノリボン及びその製造方法、ナノリボンを用いたｆｅｔ及びその製造方法、ナノリボンを用いた塩基配列決定方法およびその装置
EP2521796B1 (en) * 2010-01-04 2015-07-29 Life Technologies Corporation Dna sequencing methods and detectors and systems for carrying out the same
US8715933B2 (en) * 2010-09-27 2014-05-06 Nabsys, Inc. Assay methods using nicking endonucleases
CN102242062B (zh) * 2011-04-19 2012-12-19 浙江大学 一种高分辨率的生物传感器
CN202284206U (zh) * 2011-08-02 2012-06-27 浙江大学 一种高分辨率的生物传感器

Also Published As

Publication number Publication date
WO2014024041A1 (en) 2014-02-13
EP2879793A1 (en) 2015-06-10
JP2015528282A (ja) 2015-09-28
CN104703700B (zh) 2018-01-12
CN104703700A (zh) 2015-06-10
US20150276709A1 (en) 2015-10-01
IN2015DN01300A (cs) 2015-07-03

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