HK1210627A1 - 適合於製備 的使用蛋白酶製備粗製生物學提取物 - Google Patents

適合於製備 的使用蛋白酶製備粗製生物學提取物

Info

Publication number
HK1210627A1
HK1210627A1 HK15111314.9A HK15111314A HK1210627A1 HK 1210627 A1 HK1210627 A1 HK 1210627A1 HK 15111314 A HK15111314 A HK 15111314A HK 1210627 A1 HK1210627 A1 HK 1210627A1
Authority
HK
Hong Kong
Prior art keywords
cdna
preparing
estracts
protease
crude biological
Prior art date
Application number
HK15111314.9A
Other languages
English (en)
Inventor
Brittan L Pasloske
Hoang Quoc
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27663090&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=HK1210627(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Publication of HK1210627A1 publication Critical patent/HK1210627A1/zh

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/158Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
HK15111314.9A 2002-01-28 2015-11-17 適合於製備 的使用蛋白酶製備粗製生物學提取物 HK1210627A1 (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US35240202P 2002-01-28 2002-01-28

Publications (1)

Publication Number Publication Date
HK1210627A1 true HK1210627A1 (zh) 2016-04-29

Family

ID=27663090

Family Applications (1)

Application Number Title Priority Date Filing Date
HK15111314.9A HK1210627A1 (zh) 2002-01-28 2015-11-17 適合於製備 的使用蛋白酶製備粗製生物學提取物

Country Status (6)

Country Link
US (6) US20030170617A1 (zh)
EP (4) EP1476573B1 (zh)
AT (1) ATE454469T1 (zh)
DE (1) DE60330842D1 (zh)
HK (1) HK1210627A1 (zh)
WO (1) WO2003064605A2 (zh)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ511680A (en) 2001-05-14 2004-07-30 Univ Waikato Method for preparing nucleic acid or DNA samples and a DNA extraction process using thermophilic proteases
EP1476573B1 (en) 2002-01-28 2010-01-06 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
DK1601450T3 (da) * 2003-03-10 2013-09-08 Expression Pathology Inc Flydende vævspræparat ud fra histopatologisk bearbejdede biologiske prøver, væv og celler
CN1580283A (zh) * 2003-08-13 2005-02-16 清华大学 一种检测核酸分子的方法
US20050208501A1 (en) 2004-03-16 2005-09-22 Ambion, Inc. Process and reagents for extraction of RNA from fractionated blood leukocytes
US20050239055A1 (en) * 2004-04-22 2005-10-27 Mariani Brian D Enterovirus primers and probes
US7575864B2 (en) * 2004-05-27 2009-08-18 E.I. Du Pont De Nemours And Company Method for the direct detection of diagnostic RNA
US20050277121A1 (en) * 2004-06-11 2005-12-15 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
ATE532875T1 (de) * 2004-06-17 2011-11-15 Zygem Corp Ltd Reinigungsverfahren und ihre verwendungen
US20060188892A1 (en) * 2005-02-18 2006-08-24 Ambion, Inc. Enzymatic digestion of tissue
EP2484781B1 (en) * 2006-08-01 2017-08-23 Applied Biosystems, LLC Detection of analytes and nucleic acids
WO2008135197A1 (en) * 2007-05-03 2008-11-13 Roche Diagnostics Gmbh Improved lysis and reverse transcription for mrna quantification
US7964350B1 (en) 2007-05-18 2011-06-21 Applied Biosystems, Llc Sample preparation for in situ nucleic acid analysis
EP2310527B1 (en) * 2008-08-01 2015-04-29 Roche Diagnostics GmbH Improved lysis and reverse transcription for mrna quantification
US8211637B2 (en) * 2008-12-19 2012-07-03 Life Technologies Corporation Proteinase K inhibitors, methods and compositions therefor
WO2011104027A1 (en) 2010-02-26 2011-09-01 Qiagen Gmbh Process for parallel isolation and/or purification of rna and dna
EP2559764A1 (en) 2011-08-17 2013-02-20 Qiagen GmbH Composition and methods for RT-PCR comprising an anionic polymer
CN106471135B (zh) * 2014-04-28 2021-06-08 奎斯特诊断投资股份有限公司 肠道病毒和双埃柯病毒的分子检测
US11111487B2 (en) 2015-10-28 2021-09-07 Silicon Valley Scientific, Inc. Method and apparatus for encoding cellular spatial position information
US20170166956A1 (en) * 2015-12-11 2017-06-15 Shoreline Biome, Llc Methods for DNA Preparation for Multiplex High Throughput Targeted Sequencing
WO2017180745A1 (en) 2016-04-14 2017-10-19 T2 Biosystems, Inc. Methods and systems for amplification in complex samples
US10894990B2 (en) 2016-05-17 2021-01-19 Shoreline Biome, Llc High throughput method for identification and sequencing of unknown microbial and eukaryotic genomes from complex mixtures
EP3548603A4 (en) 2016-11-29 2020-10-28 S2 Genomics, Inc. METHOD AND DEVICE FOR PROCESSING TISSUE SAMPLES
GB201803654D0 (en) 2018-03-07 2018-04-25 Arcticzymes As Thermolabile proteinases
AU2019279085A1 (en) 2018-06-01 2021-01-07 S2 Genomics, Inc. Method and apparatus for processing tissue samples
CN108588224A (zh) * 2018-06-14 2018-09-28 大连医科大学附属第医院 一种用于嗜铬细胞瘤/副神经节瘤早期诊断和术前评估的生物标志物及其应用
EP4144863A4 (en) * 2020-04-30 2024-07-31 Takara Bio Inc METHOD FOR DETECTING RNA VIRUS
FR3116065A1 (fr) * 2020-11-12 2022-05-13 Innovative Diagnostics Genetics Détection d’acides nucléiques dans un échantillon biologique
CN113151398A (zh) * 2021-05-07 2021-07-23 广州复能基因有限公司 外泌体中核酸分子的检测方法

Family Cites Families (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0124317B1 (en) 1983-04-27 1990-04-11 Ici Americas Inc. Proline derivatives
US5194588A (en) 1985-01-22 1993-03-16 Ici Americas Inc. Aminoalcohol intermediates for peptide derivatives
US5055450A (en) 1985-01-22 1991-10-08 Ici Americas Inc. Peptide derivatives
GB8600263D0 (en) 1985-01-22 1986-02-12 Ici America Inc Peptide derivatives
US4900677A (en) * 1986-09-26 1990-02-13 E. I. Du Pont De Nemours And Company Process for rapid isolation of high molecular weight DNA
CA1317860C (en) 1987-04-01 1993-05-18 Daniel Louis Kacian Techniques for preparing specimens for bacterial assays
US5008245A (en) 1988-10-27 1991-04-16 University Of Kentucky Research Foundation Novel peptidyl carbamate inhibitors of the enzyme elastase
NO177716C (no) 1989-06-09 1995-11-08 Zeneca Ltd Fremgangsmåte for fremstilling av terapeutisk aktive polypeptider, DNA-sekvens, ekspresjonsvektor og antistoff
CA2021660A1 (en) 1989-07-26 1991-01-27 Philippe Bey Peptidase inhibitors
US5545522A (en) 1989-09-22 1996-08-13 Van Gelder; Russell N. Process for amplifying a target polynucleotide sequence using a single primer-promoter complex
US4997932A (en) * 1989-11-13 1991-03-05 Boehringer Mannheim Corporation Method and kit for purifying nucleic acids
US5284940A (en) * 1990-11-14 1994-02-08 Hri Research, Inc. Preparation for nucleic acid samples
US5284829A (en) 1991-11-26 1994-02-08 The Regents Of The University Of California Synthetic tetrapeptides for the prevention of schistosome parasite infection
KR100289793B1 (ko) 1992-01-29 2001-12-01 테츠오 토미나가 폴리누클레오티드고정지지체
AU681082B2 (en) * 1992-05-06 1997-08-21 Gen-Probe Incorporated Nucleic acid sequence amplification method, composition and kit
US5386024A (en) * 1993-02-10 1995-01-31 Gen-Probe Incorporated Method to prepare nucleic acids from a biological sample using low pH and acid protease
AU6776994A (en) 1993-05-06 1994-12-12 Abbott Laboratories Direct lysis buffer and the detection of hiv-1 plasma viremia
US5935927A (en) 1994-02-03 1999-08-10 The Picower Institute For Medical Research Compositions and methods for stimulating amyloid removal in amyloidogenic diseases using advanced glycosylation endproducts
AU699232B2 (en) * 1994-03-10 1998-11-26 Gen-Probe Incorporated Method for suppressing inhibition of enzyme-mediated reactions by ionic detergents
US5712095A (en) * 1994-06-16 1998-01-27 Becton Dickinson And Company Rapid and sensitive detection of antibiotic-resistant mycobacteria using oligonucleotide probes specific for ribosomal RNA precursors
WO1996000228A1 (en) * 1994-06-23 1996-01-04 Dade International Inc. Method for the rapid isolation of nucleic acid
US5693467A (en) 1995-05-19 1997-12-02 The American Type Culture Collection Mycoplasma polymerase chain reaction testing system using a set of mixed and single sequence primers
DE69739909D1 (de) * 1996-03-26 2010-07-29 Michael S Kopreski Methoden aus plasma oder serum extrahierte extrazelluraere rna zur diagnoseüberwachung oder evaluation von krebs verwenden
US5677124A (en) 1996-07-03 1997-10-14 Ambion, Inc. Ribonuclease resistant viral RNA standards
US5939262A (en) 1996-07-03 1999-08-17 Ambion, Inc. Ribonuclease resistant RNA preparation and utilization
US6218105B1 (en) * 1996-07-19 2001-04-17 Kathleen S. Hall High throughput papilloma virus in vitro infectivity assay
US5871628A (en) 1996-08-22 1999-02-16 The University Of Texas System Automatic sequencer/genotyper having extended spectral response
ATE438719T1 (de) * 1997-03-07 2009-08-15 Siemens Healthcare Diagnostics Spezifischer marker für prostatakrebs
AU745185B2 (en) * 1997-06-25 2002-03-14 Promega Corporation Method of isolating RNA
US6111096A (en) * 1997-10-31 2000-08-29 Bbi Bioseq, Inc. Nucleic acid isolation and purification
EP1032595B1 (en) 1997-11-18 2011-05-18 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Nucleic acids involved in the responder phenotype and applications thereof
HUP0100082A3 (en) 1998-01-26 2001-12-28 Abbott Gmbh & Co Kg Thrombin inhibitor, peptide derivatives, pharmaceutical compositions comprising thereof and their use
JP2002511446A (ja) 1998-04-08 2002-04-16 セルテック・セラピューティクス・リミテッド 脂 質
US6204375B1 (en) 1998-07-31 2001-03-20 Ambion, Inc. Methods and reagents for preserving RNA in cell and tissue samples
DE69900949T2 (de) 1998-09-18 2002-10-02 Micromet Ag Dns amplifizierung aus einzelnen zellen
US6777210B1 (en) 1998-09-24 2004-08-17 Ambion, Inc. Method and reagents for inactivating ribonucleases RNase A, RNase I and RNase T1
US6265165B1 (en) * 1998-11-12 2001-07-24 The Regents Of The University Of California Methods for EST-specific full length cDNA cloning
AU2354800A (en) 1998-12-10 2000-06-26 Genetic Vectors, Inc. Method of protein removal
DE19916534A1 (de) * 1999-04-13 2000-10-19 Gsf Forschungszentrum Umwelt RNA-Isolierungs-Kit
EP1200578B1 (en) 1999-07-23 2004-04-14 Genentech, Inc. Method for rnase- and organic solvent-free plasmid dna purification using tangential flow filtration
EP1220944B1 (en) 1999-09-24 2010-09-15 Ambion, Inc. Nuclease inhibitor cocktail
US7264932B2 (en) 1999-09-24 2007-09-04 Applera Corporation Nuclease inhibitor cocktail
US7186507B2 (en) 1999-12-09 2007-03-06 Indiana University Research And Technology Corporation Fluorescent in situ RT-PCR
US6316608B1 (en) 2000-03-20 2001-11-13 Incyte Genomics, Inc. Combined polynucleotide sequence as discrete assay endpoints
US7001724B1 (en) 2000-11-28 2006-02-21 Applera Corporation Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases
ATE324376T1 (de) 2001-01-16 2006-05-15 Invitrogen Corp Reagenz zur isolierung von rna
US20020172972A1 (en) 2001-05-15 2002-11-21 President And Fellows Of Harvard College Use of a selectively inactivatable enzyme to digest contaminating nucleic acid
WO2003002716A2 (en) 2001-06-28 2003-01-09 Mountain View Pharmaceuticals, Inc. Polymer stabilized proteinases
EP1476573B1 (en) * 2002-01-28 2010-01-06 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
US20040038213A1 (en) 2002-08-06 2004-02-26 Kwon Jai W. Genotyping by in situ PCR amplification of a polynucleotide in a tissue biopsy
US7214484B2 (en) 2002-12-17 2007-05-08 Sigma-Aldrich Co. Compositions and methods for nucleic acid extraction from biological samples
WO2004076640A2 (en) 2003-02-25 2004-09-10 Ambion, Inc. Small-molecule inhibitors of angiogenin and rnases and in vivo and in vitro methods of using same
US7067298B2 (en) 2003-03-31 2006-06-27 Ambion, Inc. Compositions and methods of using a synthetic Dnase I
US8975370B2 (en) 2003-04-04 2015-03-10 Universite De Lausanne Inhibitor proteins of a protease and use thereof
US20050069953A1 (en) 2003-09-30 2005-03-31 Ye Fang Assay solution compositions and methods for GPCR arrays
US20050277121A1 (en) 2004-06-11 2005-12-15 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
US20060141497A1 (en) 2004-10-22 2006-06-29 Finkelstein Sydney D Molecular analysis of cellular fluid and liquid cytology specimens for clinical diagnosis, characterization, and integration with microscopic pathology evaluation
US20060188892A1 (en) 2005-02-18 2006-08-24 Ambion, Inc. Enzymatic digestion of tissue
US7964350B1 (en) 2007-05-18 2011-06-21 Applied Biosystems, Llc Sample preparation for in situ nucleic acid analysis
US8211637B2 (en) 2008-12-19 2012-07-03 Life Technologies Corporation Proteinase K inhibitors, methods and compositions therefor

Also Published As

Publication number Publication date
EP2290098A1 (en) 2011-03-02
US20180340175A1 (en) 2018-11-29
US20120322112A1 (en) 2012-12-20
US20130143275A1 (en) 2013-06-06
EP2902501A1 (en) 2015-08-05
EP2163652B1 (en) 2014-12-24
US20190270988A1 (en) 2019-09-05
US9611497B2 (en) 2017-04-04
EP1476573B1 (en) 2010-01-06
WO2003064605A2 (en) 2003-08-07
EP2163652A1 (en) 2010-03-17
US10011831B2 (en) 2018-07-03
US10233444B2 (en) 2019-03-19
US20030170617A1 (en) 2003-09-11
ATE454469T1 (de) 2010-01-15
DE60330842D1 (de) 2010-02-25
EP2902501B1 (en) 2020-04-15
EP1476573A2 (en) 2004-11-17
EP1476573A4 (en) 2005-12-14
WO2003064605A3 (en) 2004-02-19
US20170218359A1 (en) 2017-08-03
EP2163652B2 (en) 2023-05-31
US10590411B2 (en) 2020-03-17

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