HK1208704A1 - Methods of incorporating an amino acid comprising a bcn group into a polypeptide using an orthogonal codon encoding it and an orthorgonal pylrs synthase bcn pylrs bcn - Google Patents
Methods of incorporating an amino acid comprising a bcn group into a polypeptide using an orthogonal codon encoding it and an orthorgonal pylrs synthase bcn pylrs bcnInfo
- Publication number
- HK1208704A1 HK1208704A1 HK15109341.0A HK15109341A HK1208704A1 HK 1208704 A1 HK1208704 A1 HK 1208704A1 HK 15109341 A HK15109341 A HK 15109341A HK 1208704 A1 HK1208704 A1 HK 1208704A1
- Authority
- HK
- Hong Kong
- Prior art keywords
- bcn
- pylrs
- orthorgonal
- synthase
- polypeptide
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/006—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length of peptides containing derivatised side chain amino acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C271/00—Derivatives of carbamic acids, i.e. compounds containing any of the groups, the nitrogen atom not being part of nitro or nitroso groups
- C07C271/06—Esters of carbamic acids
- C07C271/08—Esters of carbamic acids having oxygen atoms of carbamate groups bound to acyclic carbon atoms
- C07C271/10—Esters of carbamic acids having oxygen atoms of carbamate groups bound to acyclic carbon atoms with the nitrogen atoms of the carbamate groups bound to hydrogen atoms or to acyclic carbon atoms
- C07C271/22—Esters of carbamic acids having oxygen atoms of carbamate groups bound to acyclic carbon atoms with the nitrogen atoms of the carbamate groups bound to hydrogen atoms or to acyclic carbon atoms to carbon atoms of hydrocarbon radicals substituted by carboxyl groups
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/13—Labelling of peptides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2/00—Peptides of undefined number of amino acids; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y601/00—Ligases forming carbon-oxygen bonds (6.1)
- C12Y601/01—Ligases forming aminoacyl-tRNA and related compounds (6.1.1)
- C12Y601/01026—Pyrrolysine-tRNAPyl ligase (6.1.1.26)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C2602/00—Systems containing two condensed rings
- C07C2602/02—Systems containing two condensed rings the rings having only two atoms in common
- C07C2602/14—All rings being cycloaliphatic
- C07C2602/24—All rings being cycloaliphatic the ring system containing nine carbon atoms, e.g. perhydroindane
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1208875.3A GB201208875D0 (en) | 2012-05-18 | 2012-05-18 | Methods |
| GBGB1210303.2A GB201210303D0 (en) | 2012-06-08 | 2012-06-08 | Polypeptides and methods |
| PCT/GB2013/051249 WO2013171485A1 (en) | 2012-05-18 | 2013-05-15 | Methods of incorporating an amino acid comprising a bcn group into a polypeptide using an orthogonal codon encoding it and an orthorgonal pylrs synthase. |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HK1208704A1 true HK1208704A1 (en) | 2016-03-11 |
Family
ID=48748281
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HK15109341.0A HK1208704A1 (en) | 2012-05-18 | 2015-09-23 | Methods of incorporating an amino acid comprising a bcn group into a polypeptide using an orthogonal codon encoding it and an orthorgonal pylrs synthase bcn pylrs bcn |
Country Status (9)
| Country | Link |
|---|---|
| US (3) | US20150148525A1 (xx) |
| EP (1) | EP2852664B1 (xx) |
| JP (1) | JP6343604B2 (xx) |
| CN (2) | CN108410827B (xx) |
| BR (1) | BR112014028588B1 (xx) |
| CA (1) | CA2873793C (xx) |
| HK (1) | HK1208704A1 (xx) |
| IN (1) | IN2014DN09922A (xx) |
| WO (1) | WO2013171485A1 (xx) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6343604B2 (ja) | 2012-05-18 | 2018-06-13 | メディカル リサーチ カウンシル | ＢＣＮ基を含むアミノ酸をコードする直交性コドンと、直交性ＰｙｌＲＳ合成酵素とを使用して、ＢＣＮ基を含むアミノ酸をポリペプチドに取り込む方法 |
| CN104744293B (zh) * | 2013-12-31 | 2016-08-17 | 深圳先进技术研究院 | 胆碱类似物及其制备方法和应用、由染料分子标记的四嗪探针及其制备方法和应用 |
| GB2528227A (en) | 2014-03-14 | 2016-01-20 | Medical Res Council | Cyclopropene amino acids and methods |
| US20190077776A1 (en) * | 2015-04-30 | 2019-03-14 | Oregon State University | Reagents and methods for bioorthogonal labeling of biomolecules in living cells |
| JP6967221B2 (ja) * | 2015-08-19 | 2021-11-17 | 国立研究開発法人理化学研究所 | 非天然アミノ酸導入抗体 |
| CN105254655B (zh) * | 2015-11-20 | 2017-03-22 | 江汉大学 | 一种基于bodipy的荧光氨基酸及其合成方法与应用 |
| CN106565762B (zh) * | 2016-11-17 | 2018-10-23 | 陕西师范大学 | 一种原位产生近红外荧光的bodipy类染料及其制备和应用 |
| SG11201908547VA (en) | 2017-03-22 | 2019-10-30 | Genentech Inc | Hydrogel cross-linked hyaluronic acid prodrug compositions and methods |
| JOP20190245A1 (ar) | 2017-04-20 | 2019-10-15 | Novartis Ag | أنظمة توصيل إطلاق مستدام تتضمن روابط بلا أثر لنقطة الربط |
| US11560384B2 (en) | 2017-05-04 | 2023-01-24 | University Of Utah Research Foundation | Benzonorbornadiene derivatives and reactions thereof |
| AR111963A1 (es) * | 2017-05-26 | 2019-09-04 | Univ California | Método y moléculas |
| TW202027794A (zh) | 2018-10-03 | 2020-08-01 | 瑞士商諾華公司 | 血管生成素樣３多肽之持續遞送 |
| CN111849929B (zh) * | 2019-04-30 | 2021-05-11 | 苏州鲲鹏生物技术有限公司 | 高效引入赖氨酸衍生物的氨酰基—tRNA合成酶 |
| IL267744A (en) * | 2019-06-30 | 2019-08-29 | Nat Inst Biotechnology Negev Ltd | Labelling molecules and methods of use thereof |
| CN115701451B (zh) * | 2021-08-02 | 2023-08-01 | 宁波鲲鹏生物科技有限公司 | 高效引入赖氨酸衍生物的氨酰基-tRNA合成酶及其应用 |
Family Cites Families (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040029129A1 (en) * | 2001-10-25 | 2004-02-12 | Liangsu Wang | Identification of essential genes in microorganisms |
| EP2392923B1 (en) | 2003-06-18 | 2014-05-21 | The Scripps Research Institute | Method of producing proteins comprising unnatural amino acid |
| SI1666604T1 (sl) * | 2003-07-07 | 2008-08-31 | Scripps Research Inst | Sestavki ortogonalnih parov lizil-tRNA in aminoacil-tRNA in aminoacil-tRNA-sintetaze in njihove uporabe |
| EP1725251A4 (en) | 2003-12-18 | 2009-07-01 | Scripps Research Inst | SELECTIVE INCORPORATION OF 5-HYDROXYTRYPTOPHANE IN PROTEINS IN MAMMALIAN CELLS |
| WO2006034332A2 (en) | 2004-09-21 | 2006-03-30 | The Scripps Research Institute | In vivo incorporation of alkynyl amino acids into proteins in eubacteria |
| JP5135504B2 (ja) | 2004-10-27 | 2013-02-06 | ザ スクリプス リサーチ インスティチュート | 非天然アミノ酸のインビボ組込み用直交翻訳成分 |
| JP2007037445A (ja) | 2005-08-02 | 2007-02-15 | Institute Of Physical & Chemical Research | ｔＲＮＡ合成方法、核酸、アミノアシルｔＲＮＡ合成方法及び非天然型アミノ酸組み込み蛋白質の製造方法 |
| WO2007090198A2 (en) | 2006-02-01 | 2007-08-09 | Encode Bio, Inc. | Fluorescent assays using orthogonal trna - aminoacyl synthetase pairs |
| US20080096819A1 (en) | 2006-05-02 | 2008-04-24 | Allozyne, Inc. | Amino acid substituted molecules |
| EP2155177A2 (en) | 2007-04-30 | 2010-02-24 | Intezyne Technologies Incorporated | Modification of biological targeting groups for the treatment of cancer |
| JP5590649B2 (ja) * | 2007-09-20 | 2014-09-17 | 独立行政法人理化学研究所 | 変異体ピロリジル−ｔＲＮＡ合成酵素及びこれを用いる非天然アミノ酸組み込みタンパク質の製造方法 |
| GB0721291D0 (en) | 2007-10-30 | 2007-12-12 | Medical Res Council | Methods and compositions |
| MX2011008970A (es) * | 2009-02-25 | 2012-03-06 | Bigtec Private Ltd | Sondas e iniciadores para la deteccion de chikungunya. |
| GB2470770A (en) | 2009-06-04 | 2010-12-08 | Medical Res Council | Incorporation of unnatural amino acids having an orthogonal functional group into polypeptides using orthogonal tRNA/tRNA synthetase pairs |
| GB0917242D0 (en) * | 2009-10-01 | 2009-11-18 | Medical Res Council | Acetyl lysine incorporation with tRNA synthetase |
| GB0917240D0 (en) * | 2009-10-01 | 2009-11-18 | Medical Res Council | Incorporation of methyl lysine into poiypeptide |
| CN102971329B (zh) | 2010-04-15 | 2016-06-29 | 麦迪穆有限责任公司 | 用于治疗增殖性疾病的吡咯并苯并二氮杂卓 |
| WO2011136645A1 (en) * | 2010-04-27 | 2011-11-03 | Stichting Katholieke Universiteit, More Particularly Radboud University Nijmegen | Fused cyclooctyne compounds and their use in metal-free click reactions |
| US20120077186A1 (en) | 2010-05-07 | 2012-03-29 | Oregon Health & Science University | Use of cysteine-derived suppressor trnas for non-native amino acid incorporation |
| WO2011156686A2 (en) | 2010-06-11 | 2011-12-15 | The Regents Of The University Of Colorado, A Body Corporate | Method for synthesizing a cyclic multivalent peptide using a thiol-mediated reaction |
| CA2826041C (en) | 2011-02-03 | 2020-07-07 | Embl | Unnatural amino acids comprising a cyclooctynyl or trans-cyclooctenyl analog group and uses thereof |
| GB201110815D0 (en) * | 2011-06-24 | 2011-08-10 | Medical Res Council | Incorporation of subtituted lysines into polypeptides |
| US20130066063A1 (en) * | 2011-09-09 | 2013-03-14 | John Cooke Hodges | Bicyclo[6.1.0]non-4-yne regents for chemical modification of oligonucleotides |
| GB201201100D0 (en) * | 2012-01-20 | 2012-03-07 | Medical Res Council | Polypeptides and methods |
| JP6343604B2 (ja) | 2012-05-18 | 2018-06-13 | メディカル リサーチ カウンシル | ＢＣＮ基を含むアミノ酸をコードする直交性コドンと、直交性ＰｙｌＲＳ合成酵素とを使用して、ＢＣＮ基を含むアミノ酸をポリペプチドに取り込む方法 |
| ES2907763T3 (es) | 2012-08-31 | 2022-04-26 | Sutro Biopharma Inc | Aminoácidos modificados que comprenden un grupo azido |
| EP2898085B1 (en) | 2012-09-24 | 2019-01-23 | MedImmune Limited | Cell lines |
| GB2528227A (en) | 2014-03-14 | 2016-01-20 | Medical Res Council | Cyclopropene amino acids and methods |
| GB201419109D0 (en) | 2014-10-27 | 2014-12-10 | Medical Res Council | Incorporation of unnatural amino acids into proteins |
| WO2016154675A1 (en) * | 2015-03-27 | 2016-10-06 | The University Of Queensland | Platform for non-natural amino acid incorporation into proteins |
| GB201817444D0 (en) | 2018-10-26 | 2018-12-12 | Res & Innovation Uk | Methods and compositions |
| EP3868882A1 (en) * | 2020-02-21 | 2021-08-25 | European Molecular Biology Laboratory | Archaeal pyrrolysyl trna synthetases for orthogonal use |
-
2013
- 2013-05-15 JP JP2015512124A patent/JP6343604B2/ja active Active
- 2013-05-15 CA CA2873793A patent/CA2873793C/en active Active
- 2013-05-15 IN IN9922DEN2014 patent/IN2014DN09922A/en unknown
- 2013-05-15 CN CN201810228028.9A patent/CN108410827B/zh active Active
- 2013-05-15 WO PCT/GB2013/051249 patent/WO2013171485A1/en active Application Filing
- 2013-05-15 CN CN201380037290.0A patent/CN104718285A/zh active Pending
- 2013-05-15 US US14/401,803 patent/US20150148525A1/en not_active Abandoned
- 2013-05-15 EP EP13734818.1A patent/EP2852664B1/en active Active
- 2013-05-15 BR BR112014028588-8A patent/BR112014028588B1/pt active IP Right Grant
-
2015
- 2015-09-23 HK HK15109341.0A patent/HK1208704A1/xx unknown
-
2020
- 2020-05-12 US US16/872,826 patent/US11732001B2/en active Active
-
2023
- 2023-05-16 US US18/198,205 patent/US20230340013A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP2852664A1 (en) | 2015-04-01 |
| EP2852664B1 (en) | 2017-09-06 |
| WO2013171485A1 (en) | 2013-11-21 |
| BR112014028588A2 (pt) | 2017-06-27 |
| BR112014028588B1 (pt) | 2023-03-21 |
| US20200271658A1 (en) | 2020-08-27 |
| US11732001B2 (en) | 2023-08-22 |
| CA2873793A1 (en) | 2013-11-21 |
| CN108410827A (zh) | 2018-08-17 |
| CN104718285A (zh) | 2015-06-17 |
| US20230340013A1 (en) | 2023-10-26 |
| CN108410827B (zh) | 2023-05-09 |
| IN2014DN09922A (xx) | 2015-08-14 |
| JP6343604B2 (ja) | 2018-06-13 |
| US20150148525A1 (en) | 2015-05-28 |
| BR112014028588A8 (pt) | 2021-06-29 |
| JP2015523965A (ja) | 2015-08-20 |
| CA2873793C (en) | 2022-02-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| HK1208704A1 (en) | Methods of incorporating an amino acid comprising a bcn group into a polypeptide using an orthogonal codon encoding it and an orthorgonal pylrs synthase bcn pylrs bcn | |
| IL260045B (en) | Polypeptide structures and their uses | |
| HK1203518A1 (en) | Antibodies comprising site-specific non-natural amino acid residues, methods of their preparation and methods of their use | |
| ZA201308251B (en) | Amino acid sequence presenting fusion polypeptide and its use | |
| HK1210034A1 (en) | Modified amino acids comprising an azido group | |
| ZA201502991B (en) | Disubstituted amino acids and methods of preparation and use thereof | |
| HK1211569A1 (en) | Amino acid derivatives | |
| SG191071A1 (en) | Ion exchange chromatography in the presence of an amino acid | |
| HK1216016A1 (zh) | 鋅氨基酸絡合物與半胱氨酸 | |
| HK1199892A1 (en) | Modified mini-hepcidin peptides and methods of using thereof | |
| HK1200851A1 (en) | Etanercept formulations stabilized with amino acids | |
| ZA201206832B (en) | Novel peptides and method for their preparation and use | |
| PL2719290T3 (pl) | Mikrokapsułkowana mieszanka aminokwasów i sposób produkcji tej mieszanki | |
| HK1203161A1 (en) | Peptides and methods of using same | |
| HRP20171373T1 (hr) | Predlijek amino kiseline koja sadrži fluor | |
| EP2931311A4 (en) | LYOPHILIZED BALLULAR PELLETS OF ANTI-IL-23 ANTIBODIES | |
| EP2716652A4 (en) | FATTY ANTI-ACID SYNTHASE POLYPEPTIDE AND USE THEREOF | |
| EP2798658A4 (en) | SYSTEMS AND METHODS FOR SEQUENCING PEPTIDES BY MASS SPECTROMETRY | |
| EP2769717A4 (en) | COMPOSITION COMPRISING REDUCED GLUTATHIONE AND ACETAMINOPHEN AND PREPARATION METHOD THEREOF | |
| GB201303847D0 (en) | Amino acid composition | |
| ZA201503947B (en) | Zinc amino acid complex with cysteine | |
| AU2012902916A0 (en) | Amino acid analogues and methods for their synthesis |