HK1047158B - Spectroscopy used to confirm the target connection of complexes and related fluorescence - Google Patents

Spectroscopy used to confirm the target connection of complexes and related fluorescence Download PDF

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Publication number
HK1047158B
HK1047158B HK02108073.1A HK02108073A HK1047158B HK 1047158 B HK1047158 B HK 1047158B HK 02108073 A HK02108073 A HK 02108073A HK 1047158 B HK1047158 B HK 1047158B
Authority
HK
Hong Kong
Prior art keywords
target species
test
denaturation
test compound
isothermal
Prior art date
Application number
HK02108073.1A
Other languages
German (de)
English (en)
French (fr)
Chinese (zh)
Other versions
HK1047158A1 (en
Inventor
E. Epps Dennis
K. Tomich Paul
J. Kezdy Ferenc
K. Marschke Charles
Original Assignee
Pharmacia & Upjohn Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pharmacia & Upjohn Company filed Critical Pharmacia & Upjohn Company
Publication of HK1047158A1 publication Critical patent/HK1047158A1/en
Publication of HK1047158B publication Critical patent/HK1047158B/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/805—Optical property
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/12—Condition responsive control
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/13—Tracers or tags

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
HK02108073.1A 1999-12-09 2000-12-07 Spectroscopy used to confirm the target connection of complexes and related fluorescence HK1047158B (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/458,172 1999-12-09
US09/458,172 US6582907B1 (en) 1999-12-09 1999-12-09 Use of fluorescence correlation spectroscopy to identify compounds that bind to target species under isothermal denaturing conditions
PCT/US2000/042642 WO2001044817A2 (en) 1999-12-09 2000-12-07 Fluorescence correlation spectroscopy to identify compound-target binding

Publications (2)

Publication Number Publication Date
HK1047158A1 HK1047158A1 (en) 2003-02-07
HK1047158B true HK1047158B (en) 2004-12-03

Family

ID=23819671

Family Applications (1)

Application Number Title Priority Date Filing Date
HK02108073.1A HK1047158B (en) 1999-12-09 2000-12-07 Spectroscopy used to confirm the target connection of complexes and related fluorescence

Country Status (13)

Country Link
US (2) US6582907B1 (https=)
EP (1) EP1236047B1 (https=)
JP (1) JP2003517609A (https=)
AT (1) ATE265684T1 (https=)
AU (1) AU777163B2 (https=)
CA (1) CA2392239A1 (https=)
DE (1) DE60010323T2 (https=)
DK (1) DK1236047T3 (https=)
ES (1) ES2218280T3 (https=)
HK (1) HK1047158B (https=)
PT (1) PT1236047E (https=)
TR (1) TR200401075T4 (https=)
WO (1) WO2001044817A2 (https=)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9425138D0 (en) * 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US6517781B1 (en) * 1997-06-02 2003-02-11 Aurora Biosciences Corporation Low fluorescence assay platforms and related methods for drug discovery
DE10013854A1 (de) * 2000-03-17 2001-09-20 Zeiss Carl Jena Gmbh Anordnung und Verfahren zur Untersuchung von Wirkstoffen zur Beeinflussung intrazellulärer Vorgänge
JP2005538929A (ja) 2002-01-16 2005-12-22 ダイナル バイオテック エイエスエイ 単一サンプルからの核酸及びタンパク質の単離方法
EP1526373B1 (en) * 2002-06-21 2013-11-27 Olympus Corporation Biomolecule analyzer
EP1527333A1 (en) * 2002-08-01 2005-05-04 Sensor Technologies LLC Fluorescence correlation spectroscopy instrument
GB0229287D0 (en) * 2002-12-16 2003-01-22 Dna Res Innovations Ltd Polyfunctional reagents
WO2006013109A1 (en) * 2004-08-06 2006-02-09 Epfl Swiss Federal Institute Of Technology-Lausanne Method for testing a substance interacting with a target molecule
EP1848983B1 (en) * 2005-02-15 2013-03-27 Tata Institute of Fundamental Research Fluorescence correlation microscopy with real-time alignment readout
DE102007045666B4 (de) * 2007-09-25 2026-04-02 Carl Zeiss Microscopy Gmbh Verfahren und Anordnung zur Ausgabe von Restfehlern einer an eine Punktemenge angepassten Funktion
KR100963305B1 (ko) 2008-01-23 2010-06-11 건국대학교 산학협력단 단백질 내의 도메인 간 변성과정 분석방법
US12400741B2 (en) * 2020-02-28 2025-08-26 Howard University Selective extraction of late exponentials

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5585277A (en) 1993-06-21 1996-12-17 Scriptgen Pharmaceuticals, Inc. Screening method for identifying ligands for target proteins
US5679582A (en) 1993-06-21 1997-10-21 Scriptgen Pharmaceuticals, Inc. Screening method for identifying ligands for target proteins
CA2184195C (en) 1995-10-25 2002-04-16 Andrew Pakula Screening method for identifying ligands for target proteins
JP2000502440A (ja) 1995-12-07 2000-02-29 スクリプトジェン・ファーマスーティカルズ，インコーポレイテッド リガンドを識別するための蛍光に基づくスクリーニング方法
AU741049B2 (en) 1996-05-09 2001-11-22 Life Technologies Corporation Microplate thermal shift assay and apparatus for ligand development and multi-variable protein chemistry optimization
EP0836090A1 (en) 1996-10-12 1998-04-15 Evotec BioSystems GmbH Method of analysis of samples by determination of the distribution of specific brightnesses of particles
DE69811724T2 (de) 1997-12-05 2003-10-16 Pharmacia & Upjohn Co., Kalamazoo Fluoreszenz-basierte hts-(high throughput screening)assayverfahren für proteinkinase und phosphatase
US6242190B1 (en) 1999-12-01 2001-06-05 John Hopkins University Method for high throughput thermodynamic screening of ligands
US6376180B1 (en) * 1999-12-09 2002-04-23 Pharmacia & Upjohn Company Methods of identifying compounds that bind to target species under isothermal denaturing conditions

Also Published As

Publication number Publication date
EP1236047A2 (en) 2002-09-04
HK1047158A1 (en) 2003-02-07
DE60010323D1 (de) 2004-06-03
JP2003517609A (ja) 2003-05-27
EP1236047B1 (en) 2004-04-28
AU777163B2 (en) 2004-10-07
WO2001044817A2 (en) 2001-06-21
CA2392239A1 (en) 2001-06-21
AU4519801A (en) 2001-06-25
ATE265684T1 (de) 2004-05-15
ES2218280T3 (es) 2004-11-16
US20030219820A1 (en) 2003-11-27
TR200401075T4 (tr) 2004-07-21
PT1236047E (pt) 2004-08-31
WO2001044817A3 (en) 2002-05-10
US6582907B1 (en) 2003-06-24
DK1236047T3 (da) 2004-07-05
DE60010323T2 (de) 2005-05-25

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Legal Events

Date Code Title Description
PF Patent in force
PC Patent ceased (i.e. patent has lapsed due to the failure to pay the renewal fee)

Effective date: 20071207