GR35850B - Μεθοδος δια την παρασκευην λυσοζυμης. - Google Patents

Μεθοδος δια την παρασκευην λυσοζυμης.

Info

Publication number
GR35850B
GR35850B GR670135850A GR670135850A GR35850B GR 35850 B GR35850 B GR 35850B GR 670135850 A GR670135850 A GR 670135850A GR 670135850 A GR670135850 A GR 670135850A GR 35850 B GR35850 B GR 35850B
Authority
GR
Greece
Prior art keywords
lysozyme
salt
resin
eluted
egg white
Prior art date
Application number
GR670135850A
Other languages
English (en)
Inventor
Giuseppe Chielmetti
Carlo Trinchera
Original Assignee
S.P.A. Societa Prodotti Antibiotici S.P.A.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by S.P.A. Societa Prodotti Antibiotici S.P.A. filed Critical S.P.A. Societa Prodotti Antibiotici S.P.A.
Publication of GR35850B publication Critical patent/GR35850B/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y10S435/815—Enzyme separation or purification by sorption

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

έθοδος δια την παραγωγήν λυσοζύμης εκ λευκού του ωού, εις την οποίαν το λευκόν του ωού έρχεται εις επαφήν μετά μιας ασθενούς οξίνου ιοντοεναλλακτικήςρητίνης εις εν ρΗ 6-7 εις μίαν θερμοκρασίαν κάτω της θερμοκρασίας δωματίου, διαχωρίζεται η ρητίνη,εκλούονται αι ρυπαίνουσαι πρωτείναι εκ της ρητίνης μεθ' ενός διαλύματος άλατος έχοντος μίαν συγκέντρωσιν ουχί μεγαλυτέραν του 0.2 Μ και εν ρΗ ουχί μεγαλύτερον του 7, εκλούεται ακολούθως η λυσοζύμη μεθ'ενός υδατικού διαλύματος ενός άλατος και καταβυθίζεται η λυσοζύμη εκ του εκλούσματος δι' αυξήσεως της συγκεντρώσεως του άλατος εντός αυτής.
GR670135850A 1966-02-28 1967-02-27 Μεθοδος δια την παρασκευην λυσοζυμης. GR35850B (el)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB8675/66A GB1110466A (en) 1966-02-28 1966-02-28 Process for the production of lysozyme

Publications (1)

Publication Number Publication Date
GR35850B true GR35850B (el) 1968-11-11

Family

ID=9857054

Family Applications (1)

Application Number Title Priority Date Filing Date
GR670135850A GR35850B (el) 1966-02-28 1967-02-27 Μεθοδος δια την παρασκευην λυσοζυμης.

Country Status (11)

Country Link
US (1) US3515643A (el)
JP (1) JPS5138791B1 (el)
BE (1) BE694538A (el)
CH (1) CH488746A (el)
DE (1) DE1617805C3 (el)
DK (1) DK113841B (el)
ES (1) ES337291A1 (el)
FR (1) FR1514474A (el)
GB (1) GB1110466A (el)
GR (1) GR35850B (el)
NL (1) NL6702515A (el)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4945590B1 (el) * 1970-12-26 1974-12-05
JPS6038112B2 (ja) * 1982-05-29 1985-08-30 キユーピー株式会社 卵白リゾチ−ムの溶出方法
JPS58209977A (ja) * 1982-05-31 1983-12-07 Q P Corp 卵白中のリゾチ−ムを吸着分取するための装置
JPS6019991B2 (ja) * 1982-06-02 1985-05-18 キユーピー株式会社 卵白リゾチ−ムの結晶化方法
DE3587307T2 (de) * 1984-12-06 1993-09-02 Fina Research Transformierte hefen die lysozym produzieren, fuer diese transformation zu verwendende plasmide und deren verwendung.
CA1283072C (en) * 1986-12-01 1991-04-16 Timothy Durance Process for the isolation and separation of lysozyme and avidin from eggwhite
JP2006515559A (ja) * 2002-05-23 2006-06-01 インディアン インスティチュート オブ テクノロジー 糖タンパク質であるアビジンを単離および精製するプロセス
US7906630B2 (en) * 2004-04-27 2011-03-15 PerkinElmer Heath Sciences, Inc. Method for identifying peptides in a biological sample
US20060286086A1 (en) * 2005-06-21 2006-12-21 Saint Simeon Lda Lysozyme-based food stuff
KR20230009419A (ko) * 2020-05-11 2023-01-17 바이오수티카 비.브이. 암탉 난백으로부터 단백질을 정제하는 방법 및 sars-cov-2에 대한 항바이러스제로서 이의 용도
IT202000016663A1 (it) * 2020-07-09 2022-01-09 Bioseutica Bv Nuovo processo di purificazione delle proteine dall’albume di uovo ed uso delle stesse come agenti antivirali contro sars-cov-2

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3419471A (en) * 1965-04-19 1968-12-31 Eisai Co Ltd Preparation of albumin-free lysozyme

Also Published As

Publication number Publication date
JPS5138791B1 (el) 1976-10-23
FR1514474A (fr) 1968-02-23
BE694538A (el) 1967-07-31
ES337291A1 (es) 1968-02-16
CH488746A (de) 1970-04-15
DK113841B (da) 1969-05-05
US3515643A (en) 1970-06-02
NL6702515A (el) 1967-08-29
DE1617805C3 (de) 1974-08-22
DE1617805A1 (de) 1971-04-08
GB1110466A (en) 1968-04-18
DE1617805B2 (de) 1974-01-24

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