GR3023287T3 - Enzymatic synthesis of oligonucleotides - Google Patents

Enzymatic synthesis of oligonucleotides

Info

Publication number
GR3023287T3
GR3023287T3 GR970400952T GR970400952T GR3023287T3 GR 3023287 T3 GR3023287 T3 GR 3023287T3 GR 970400952 T GR970400952 T GR 970400952T GR 970400952 T GR970400952 T GR 970400952T GR 3023287 T3 GR3023287 T3 GR 3023287T3
Authority
GR
Greece
Prior art keywords
template
sequence
present
oligonucleotides
catalytic
Prior art date
Application number
GR970400952T
Other languages
English (en)
Inventor
Rodney M Richards
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amgen Inc filed Critical Amgen Inc
Publication of GR3023287T3 publication Critical patent/GR3023287T3/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/582—Recycling of unreacted starting or intermediate materials

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
GR970400952T 1990-09-21 1997-04-29 Enzymatic synthesis of oligonucleotides GR3023287T3 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US58636890A 1990-09-08 1990-09-08
US76214091A 1991-09-23 1991-09-23
PCT/US1991/006887 WO1992005287A1 (en) 1990-09-21 1991-09-23 Enzymatic synthesis of oligonucleotides

Publications (1)

Publication Number Publication Date
GR3023287T3 true GR3023287T3 (en) 1997-07-30

Family

ID=27079687

Family Applications (1)

Application Number Title Priority Date Filing Date
GR970400952T GR3023287T3 (en) 1990-09-21 1997-04-29 Enzymatic synthesis of oligonucleotides

Country Status (12)

Country Link
US (1) US5650271A (el)
EP (1) EP0502180B1 (el)
JP (1) JPH05502594A (el)
AT (1) ATE151815T1 (el)
AU (1) AU648842B2 (el)
CA (1) CA2069096A1 (el)
DE (1) DE69125702T2 (el)
DK (1) DK0502180T3 (el)
ES (1) ES2100963T3 (el)
GR (1) GR3023287T3 (el)
HK (1) HK1007170A1 (el)
WO (1) WO1992005287A1 (el)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5645987A (en) * 1990-09-21 1997-07-08 Amgen Inc. Enzymatic synthesis of oligonucleotides
CA2131630A1 (en) * 1992-03-10 1993-09-30 Yury E. Khudyakov Exchangeable template reaction
US5648211A (en) * 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US5700642A (en) * 1995-05-22 1997-12-23 Sri International Oligonucleotide sizing using immobilized cleavable primers
US5830655A (en) * 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers
US5916777A (en) * 1995-06-07 1999-06-29 Gen-Probe Incorporated Enzymatic synthesis of oligonucleotides using 3'-ribonucleotide primers
US5932450A (en) * 1995-06-07 1999-08-03 Gen-Probe Incorporated Enzymatic synthesis of oligonucleotides using digestible templates
US5739311A (en) * 1995-06-07 1998-04-14 Gen-Probe Incorporated Enzymatic synthesis of phosphorothioate oligonucleotides using restriction endonucleases
US5652126A (en) * 1995-06-07 1997-07-29 Gen-Probe Incorporated Use of restriction endonuclease sequences for cleaving phosphorothioate oligonucleotides
WO1996040901A1 (en) * 1995-06-07 1996-12-19 Gen-Probe Incorporated Template and primer based synthesis of enzymatically cleavable oligonucleotides
US6025133A (en) * 1996-12-30 2000-02-15 Gen-Probe Incorporated Promoter-sequestered oligonucleoside and method of use
US20030165888A1 (en) * 2001-07-18 2003-09-04 Brown Bob D. Oligonucleotide probes and primers comprising universal bases for diagnostic purposes
US6518017B1 (en) * 1997-10-02 2003-02-11 Oasis Biosciences Incorporated Combinatorial antisense library
US6951719B1 (en) * 1999-08-11 2005-10-04 Proteus S.A. Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained
US20030104417A1 (en) * 1998-08-12 2003-06-05 Proteus S.A. Template-mediated, ligation-oriented method of nonrandomly shuffling polynucleotides
EP1171634B1 (en) * 1999-04-08 2005-10-26 Gen-Probe Incorporated Amplification and sequencing primer pairs and use thereof
CA2365984A1 (en) * 1999-04-08 2000-10-19 Oasis Biosciences, Inc. Antisense oligonucleotides comprising universal and/or degenerate bases
US7125982B1 (en) 2001-12-05 2006-10-24 Frayne Consultants Microbial production of nuclease resistant DNA, RNA, and oligo mixtures
US20070238108A1 (en) * 2006-04-07 2007-10-11 Agilent Technologies, Inc. Validation of comparative genomic hybridization
US10604790B2 (en) * 2014-12-24 2020-03-31 Abbott Laboratories Sequence conversion and signal amplifier DNA cascade reactions and detection methods using same

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59219235A (ja) * 1983-05-30 1984-12-10 Mitsui Toatsu Chem Inc 消化性潰瘍用剤
US4795700A (en) * 1985-01-25 1989-01-03 California Institute Of Technology Nucleic acid probes and methods of using same
US4994368A (en) * 1987-07-23 1991-02-19 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
DE68911648T2 (de) * 1988-03-24 1994-06-23 Univ Iowa Res Found Katalytische hybridisierungs-systeme zum nachweis von nukleinsäuresequenzen, die auf deren aktivität als kofaktoren in katalytischen reaktionen basieren in denen eine komplementäre, markierte nukleinsäureprobe gespalten wird.
US5132215A (en) * 1988-09-15 1992-07-21 Eastman Kodak Company Method of making double-stranded dna sequences
EP0542874A4 (en) * 1990-07-25 1994-05-11 Syngene Inc Circular extension for generating multiple nucleic acid complements
US5455166A (en) * 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification

Also Published As

Publication number Publication date
JPH05502594A (ja) 1993-05-13
DE69125702D1 (de) 1997-05-22
EP0502180A1 (en) 1992-09-09
EP0502180B1 (en) 1997-04-16
DK0502180T3 (da) 1997-09-22
EP0502180A4 (en) 1993-03-17
CA2069096A1 (en) 1992-03-22
AU8844691A (en) 1992-04-15
DE69125702T2 (de) 1997-11-27
ES2100963T3 (es) 1997-07-01
WO1992005287A1 (en) 1992-04-02
AU648842B2 (en) 1994-05-05
ATE151815T1 (de) 1997-05-15
HK1007170A1 (en) 1999-04-01
US5650271A (en) 1997-07-22

Similar Documents

Publication Publication Date Title
GR3023287T3 (en) Enzymatic synthesis of oligonucleotides
DK0593422T3 (da) Fremgangsmåde til syntetisering af oligonukleotider, som kan tilpasses til syntese i stor skala
ES8800357A1 (es) Un procedimiento para sintetizar un fragmento de acido nucleico
UA49843C2 (uk) Олігонуклеотид для ампліфікації послідовностей нуклеїнових кислот, спосіб ампліфікації цільової послідовності нуклеїнової кислоти, набір для здійснення реакції ампліфікації нуклеїнової кислоти
WO1993016094A3 (en) Applications of fluorescent n-nucleosides and fluorescent structural analogs of n-nucleosides
WO1997000330A3 (de) Verfahren zur transkriptionsfreien amplifizierung von nukleinsäuren
ES2131699T3 (es) Procedimiento mejorado de cuantificacion del acido nucleico.
Ohtsuka et al. Joining of synthetic ribotrinucleotides with defined sequences catalyzed by T4 RNA ligase
Fairfield et al. On the processivity of DNA replication
DK0836612T3 (da) Nye synthoner til stereoselektiv oligonucleotidsyntese
ES454538A1 (es) Un proceso para realizar una reaccion.
Letsinger et al. Incorporation of 5'-amino-5'-deoxythymidine 5'-phosphate in polynucleotides by use of DNA polymerase I and a φX174 DNA template
ATE130634T1 (de) Enzymatisches amplifizierungsverfahren in vitro.
Rammler Periodate oxidations of enamines. I. Oxidation of adenosine 5'-monophosphate in the presence of methylamine
ES453775A1 (es) Procedimiento para la preparacion de n,n'-bis-trimetilsili- lurea.
NL7203123A (en) Riboside derivs from bacillus megaterium
ES390618A1 (es) Procedimiento para la polimerizacion en masa de acriloni- trilo.
Berthet et al. Chemical synthesis, DNA incorporation and biological study of a new photocleavable 2′-deoxyadenosine mimic
HU912936D0 (en) Method for producing oligonucleotides by chemical way
Maitra The identification of 3′-hydroxyl nucleoside terminus of RNA chains released in the DNA-dependent RNA polymerase reaction
AU5337690A (en) Method for stabilizing the hybridization of complementary polynucleotide sequences
MX9602980A (es) Proceso mejorado para la sintesis de compuestos de dioxovinilo.
Maksimenko et al. The Use of Chemical Ligation for Investigation of the Structure of Duplexes Formed by Linear and Circular Oligonucleotides
KR930018032A (ko) 복합효소를 이용한 l-오르니친의 제조방법
JPH03502404A (ja) クラス２制限エンドヌクレアーゼ　Ｍａｍ　１