GR1006956B - Method for the detection and evaluation of the activity of chemical compositions or extracts on in-vitro cellular systems - Google Patents

Method for the detection and evaluation of the activity of chemical compositions or extracts on in-vitro cellular systems

Info

Publication number
GR1006956B
GR1006956B GR20050100239A GR20050100239A GR1006956B GR 1006956 B GR1006956 B GR 1006956B GR 20050100239 A GR20050100239 A GR 20050100239A GR 20050100239 A GR20050100239 A GR 20050100239A GR 1006956 B GR1006956 B GR 1006956B
Authority
GR
Greece
Prior art keywords
evaluation
detection
activity
extracts
chemical compositions
Prior art date
Application number
GR20050100239A
Other languages
Greek (el)
Other versions
GR20050100239A (en
Inventor
Χαραλαμπος Ι. Πρατσινης
Δημητριος Π. Κλετσας
Original Assignee
Εθνικο Κεντρο Ερευνας Φυσικων Επιστημων "Δημοκριτος",
Δημητριος Π. Κλετσας
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Εθνικο Κεντρο Ερευνας Φυσικων Επιστημων "Δημοκριτος",, Δημητριος Π. Κλετσας filed Critical Εθνικο Κεντρο Ερευνας Φυσικων Επιστημων "Δημοκριτος",
Priority to GR20050100239A priority Critical patent/GR1006956B/en
Publication of GR20050100239A publication Critical patent/GR20050100239A/en
Publication of GR1006956B publication Critical patent/GR1006956B/en

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/55Specular reflectivity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
    • G01N21/79Photometric titration
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N31/00Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
    • G01N31/22Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using chemical indicators
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing

Abstract

The present invention discloses an original method for the detection and evaluation of molecules with antioxidant or/and cellular toxic/cellular static activity as well as molecules-regulators of the collagen composition. It regards the use of the HVTs-SM1 cellular system for the determination of the quantity of the endocellular Reactive Oxygen Species (ROS).
GR20050100239A 2005-05-17 2005-05-17 Method for the detection and evaluation of the activity of chemical compositions or extracts on in-vitro cellular systems GR1006956B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GR20050100239A GR1006956B (en) 2005-05-17 2005-05-17 Method for the detection and evaluation of the activity of chemical compositions or extracts on in-vitro cellular systems

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GR20050100239A GR1006956B (en) 2005-05-17 2005-05-17 Method for the detection and evaluation of the activity of chemical compositions or extracts on in-vitro cellular systems

Publications (2)

Publication Number Publication Date
GR20050100239A GR20050100239A (en) 2006-12-18
GR1006956B true GR1006956B (en) 2010-09-06

Family

ID=38109750

Family Applications (1)

Application Number Title Priority Date Filing Date
GR20050100239A GR1006956B (en) 2005-05-17 2005-05-17 Method for the detection and evaluation of the activity of chemical compositions or extracts on in-vitro cellular systems

Country Status (1)

Country Link
GR (1) GR1006956B (en)

Also Published As

Publication number Publication date
GR20050100239A (en) 2006-12-18

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