GB967848A - Improvements in or relating to the fermentative preparation of protease - Google Patents

Improvements in or relating to the fermentative preparation of protease

Info

Publication number
GB967848A
GB967848A GB2153863A GB2153863A GB967848A GB 967848 A GB967848 A GB 967848A GB 2153863 A GB2153863 A GB 2153863A GB 2153863 A GB2153863 A GB 2153863A GB 967848 A GB967848 A GB 967848A
Authority
GB
United Kingdom
Prior art keywords
protease
medium
relating
fermentative preparation
sources
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB2153863A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shionogi and Co Ltd
Original Assignee
Shionogi and Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shionogi and Co Ltd filed Critical Shionogi and Co Ltd
Publication of GB967848A publication Critical patent/GB967848A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Protease is prepared by cultivating Pseudomonas aeruginosa under aerobic conditions in a synthetic nutrient medium containing 3-15% w/v of glucose, 0.05 to 5% w/v calcium or strontium carbonate and less than 0.5% w/v organic nitrogen sources and thereafter extracting the enzyme from the medium by methods known per se e.g. salting out, dialysis, precipitation, elution, chromatography followed by further purification. Preferably the medium also contains sources of ammonium, phosphate and magnesium ions.
GB2153863A 1962-06-01 1963-05-29 Improvements in or relating to the fermentative preparation of protease Expired GB967848A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2264362 1962-06-01

Publications (1)

Publication Number Publication Date
GB967848A true GB967848A (en) 1964-08-26

Family

ID=12088512

Family Applications (1)

Application Number Title Priority Date Filing Date
GB2153863A Expired GB967848A (en) 1962-06-01 1963-05-29 Improvements in or relating to the fermentative preparation of protease

Country Status (1)

Country Link
GB (1) GB967848A (en)

Similar Documents

Publication Publication Date Title
ES360055A1 (en) Preparation of proteolytic enzymes having maximum activity at high alkalinity
GB1097908A (en) A fermentation process for the production of l-threonine
GB967848A (en) Improvements in or relating to the fermentative preparation of protease
GB1297243A (en)
GB1011659A (en) Preparation of l-citrulline by fermentation
ES438413A1 (en) Lipolytic enzyme recovery method
GB1435980A (en) Method of producing l-arginine by fermentation
GB1111804A (en) Improvements in or relating to the preparation of proteinases
GB1029978A (en) Improvements in or relating to the production of proteolytic enzymes
GB1378408A (en) Method of preparation 1-beta-d or l-arabinofuranosylcytosine
FR2273067A1 (en) Bacterial cell treatment - by autolysis in absence of added enzymes
GB996906A (en) Improvements in or relating to the production of glutamic acid and/or alpha-ketoglutaric acid
GB1143048A (en) Improvements in the production of guanosine
GB1168834A (en) Improvements in or relating to the production of Micro-Organisms
IE33303L (en) L-asparaginase
GB1161722A (en) Improvements in or relating to the Preparation of Enzyme Solutions
KR830006432A (en) Preparation of Heteropolysaccharide S-119
JPS54119091A (en) Preparation of l-tryptophan by fermentation
GB1278558A (en) PROCESS FOR THE PREPARATION OF L-beta-(3,4-DIHYDROXYPHENYL)-alpha-ALANINE BY FERMENTATION
ES393771A1 (en) Process for the manufacture of zeaxanthin
GB1394849A (en) 5-dihydrocoriolin c and process for producing the same
ES419574A1 (en) Antibiotic bm123 and production thereof
GB1115550A (en) Process for the preparation of 5-aminovaleric acid
GB1222904A (en) Process for producing l-tryptophan
DE3173906D1 (en) An antianaemic agent and a process for producing the same