FR2273067A1 - Bacterial cell treatment - by autolysis in absence of added enzymes - Google Patents
Bacterial cell treatment - by autolysis in absence of added enzymesInfo
- Publication number
- FR2273067A1 FR2273067A1 FR7418946A FR7418946A FR2273067A1 FR 2273067 A1 FR2273067 A1 FR 2273067A1 FR 7418946 A FR7418946 A FR 7418946A FR 7418946 A FR7418946 A FR 7418946A FR 2273067 A1 FR2273067 A1 FR 2273067A1
- Authority
- FR
- France
- Prior art keywords
- autolysis
- absence
- bacterial cell
- cell treatment
- added enzymes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/005—Glycopeptides, glycoproteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- General Chemical & Material Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Mycology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Bacterial cells are treated, esp. to extract peptidoglycanes, by adjusting pH of medium to provoke autolysis in absence of added enzymes. Partic. applicable to gram-negative bacteria; cytoplasmic cell matl. is selectively destroyed. Yields and purity are higher than in known methods using enzymes. Pref. cells are used when they are in the logarithmic growth rate region. Prod. can be treated with proteolytic enzyme and pH is below 5 or above 9, pref. above 10.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR7418946A FR2273067A1 (en) | 1974-05-31 | 1974-05-31 | Bacterial cell treatment - by autolysis in absence of added enzymes |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR7418946A FR2273067A1 (en) | 1974-05-31 | 1974-05-31 | Bacterial cell treatment - by autolysis in absence of added enzymes |
Publications (2)
Publication Number | Publication Date |
---|---|
FR2273067A1 true FR2273067A1 (en) | 1975-12-26 |
FR2273067B1 FR2273067B1 (en) | 1976-10-15 |
Family
ID=9139514
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FR7418946A Granted FR2273067A1 (en) | 1974-05-31 | 1974-05-31 | Bacterial cell treatment - by autolysis in absence of added enzymes |
Country Status (1)
Country | Link |
---|---|
FR (1) | FR2273067A1 (en) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0013851A1 (en) * | 1978-12-19 | 1980-08-06 | Pierre Fabre S.A. | Purified bacterial proteoglycans, process for their preparation and vaccine containing them |
FR2455893A1 (en) * | 1979-05-08 | 1980-12-05 | Kirin Brewery | PHYSIOLOGICALLY ACTIVE POLYSACCHARIDES, THEIR PREPARATION AND THEIR APPLICATIONS |
EP0078556A1 (en) * | 1981-11-03 | 1983-05-11 | Shell Internationale Researchmaatschappij B.V. | Process for cell disruption |
EP0145233A3 (en) * | 1983-11-23 | 1985-07-03 | Imperial Chemical Industries Plc | Separation process |
CN113755365A (en) * | 2021-08-11 | 2021-12-07 | 天津科技大学 | Preparation method and application of lactobacillus extract |
-
1974
- 1974-05-31 FR FR7418946A patent/FR2273067A1/en active Granted
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0013851A1 (en) * | 1978-12-19 | 1980-08-06 | Pierre Fabre S.A. | Purified bacterial proteoglycans, process for their preparation and vaccine containing them |
FR2455893A1 (en) * | 1979-05-08 | 1980-12-05 | Kirin Brewery | PHYSIOLOGICALLY ACTIVE POLYSACCHARIDES, THEIR PREPARATION AND THEIR APPLICATIONS |
EP0078556A1 (en) * | 1981-11-03 | 1983-05-11 | Shell Internationale Researchmaatschappij B.V. | Process for cell disruption |
EP0145233A3 (en) * | 1983-11-23 | 1985-07-03 | Imperial Chemical Industries Plc | Separation process |
US4910145A (en) * | 1983-11-23 | 1990-03-20 | Imperial Chemical Industries Plc | Separation process |
CN113755365A (en) * | 2021-08-11 | 2021-12-07 | 天津科技大学 | Preparation method and application of lactobacillus extract |
CN113755365B (en) * | 2021-08-11 | 2024-02-27 | 天津科技大学 | Preparation method and application of lactobacillus extract |
Also Published As
Publication number | Publication date |
---|---|
FR2273067B1 (en) | 1976-10-15 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
ST | Notification of lapse |