FR2273067A1 - Bacterial cell treatment - by autolysis in absence of added enzymes - Google Patents

Bacterial cell treatment - by autolysis in absence of added enzymes

Info

Publication number
FR2273067A1
FR2273067A1 FR7418946A FR7418946A FR2273067A1 FR 2273067 A1 FR2273067 A1 FR 2273067A1 FR 7418946 A FR7418946 A FR 7418946A FR 7418946 A FR7418946 A FR 7418946A FR 2273067 A1 FR2273067 A1 FR 2273067A1
Authority
FR
France
Prior art keywords
autolysis
absence
bacterial cell
cell treatment
added enzymes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7418946A
Other languages
French (fr)
Other versions
FR2273067B1 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bpifrance Financement SA
Original Assignee
Agence National de Valorisation de la Recherche ANVAR
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agence National de Valorisation de la Recherche ANVAR filed Critical Agence National de Valorisation de la Recherche ANVAR
Priority to FR7418946A priority Critical patent/FR2273067A1/en
Publication of FR2273067A1 publication Critical patent/FR2273067A1/en
Application granted granted Critical
Publication of FR2273067B1 publication Critical patent/FR2273067B1/fr
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Bacterial cells are treated, esp. to extract peptidoglycanes, by adjusting pH of medium to provoke autolysis in absence of added enzymes. Partic. applicable to gram-negative bacteria; cytoplasmic cell matl. is selectively destroyed. Yields and purity are higher than in known methods using enzymes. Pref. cells are used when they are in the logarithmic growth rate region. Prod. can be treated with proteolytic enzyme and pH is below 5 or above 9, pref. above 10.
FR7418946A 1974-05-31 1974-05-31 Bacterial cell treatment - by autolysis in absence of added enzymes Granted FR2273067A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR7418946A FR2273067A1 (en) 1974-05-31 1974-05-31 Bacterial cell treatment - by autolysis in absence of added enzymes

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7418946A FR2273067A1 (en) 1974-05-31 1974-05-31 Bacterial cell treatment - by autolysis in absence of added enzymes

Publications (2)

Publication Number Publication Date
FR2273067A1 true FR2273067A1 (en) 1975-12-26
FR2273067B1 FR2273067B1 (en) 1976-10-15

Family

ID=9139514

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7418946A Granted FR2273067A1 (en) 1974-05-31 1974-05-31 Bacterial cell treatment - by autolysis in absence of added enzymes

Country Status (1)

Country Link
FR (1) FR2273067A1 (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0013851A1 (en) * 1978-12-19 1980-08-06 Pierre Fabre S.A. Purified bacterial proteoglycans, process for their preparation and vaccine containing them
FR2455893A1 (en) * 1979-05-08 1980-12-05 Kirin Brewery PHYSIOLOGICALLY ACTIVE POLYSACCHARIDES, THEIR PREPARATION AND THEIR APPLICATIONS
EP0078556A1 (en) * 1981-11-03 1983-05-11 Shell Internationale Researchmaatschappij B.V. Process for cell disruption
EP0145233A3 (en) * 1983-11-23 1985-07-03 Imperial Chemical Industries Plc Separation process
CN113755365A (en) * 2021-08-11 2021-12-07 天津科技大学 Preparation method and application of lactobacillus extract

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0013851A1 (en) * 1978-12-19 1980-08-06 Pierre Fabre S.A. Purified bacterial proteoglycans, process for their preparation and vaccine containing them
FR2455893A1 (en) * 1979-05-08 1980-12-05 Kirin Brewery PHYSIOLOGICALLY ACTIVE POLYSACCHARIDES, THEIR PREPARATION AND THEIR APPLICATIONS
EP0078556A1 (en) * 1981-11-03 1983-05-11 Shell Internationale Researchmaatschappij B.V. Process for cell disruption
EP0145233A3 (en) * 1983-11-23 1985-07-03 Imperial Chemical Industries Plc Separation process
US4910145A (en) * 1983-11-23 1990-03-20 Imperial Chemical Industries Plc Separation process
CN113755365A (en) * 2021-08-11 2021-12-07 天津科技大学 Preparation method and application of lactobacillus extract
CN113755365B (en) * 2021-08-11 2024-02-27 天津科技大学 Preparation method and application of lactobacillus extract

Also Published As

Publication number Publication date
FR2273067B1 (en) 1976-10-15

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Legal Events

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