GB924815A - Improvements in methods of preparation of polypeptides of spasmolytic activity - Google Patents
Improvements in methods of preparation of polypeptides of spasmolytic activityInfo
- Publication number
- GB924815A GB924815A GB43896/59A GB4389659A GB924815A GB 924815 A GB924815 A GB 924815A GB 43896/59 A GB43896/59 A GB 43896/59A GB 4389659 A GB4389659 A GB 4389659A GB 924815 A GB924815 A GB 924815A
- Authority
- GB
- United Kingdom
- Prior art keywords
- broth
- polypeptide
- polypeptides
- alcohol
- precipitate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/26—Lymph; Lymph nodes; Thymus; Spleen; Splenocytes; Thymocytes
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Virology (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Polypeptides having spasmolytic activity are obtained from animal organs of lymphoidal structure e.g. lymph ganglions of oxen or sheep or calf or lamb thymus, by hydrolysing the comminuted organs to a broth by warming with an aqueous solution of a protease e.g. papain, heating the broth to destroy the enzyme, clarifying the broth and fractionally precipitating the polypeptide from the clarified broth with alcohol, the active material being recovered from the product precipitated with the greatest concentration of alcohol. The hydrolysis may be carried out at 60 DEG C. for 15 minutes at a pH of 7.0. After filtration, the clarified broth may be treated with an equal volume of 90% ethanol, the precipitate discarded, and seven volumes of absolute ethanol added to precipitate the polypeptide. The crude polypeptide may be purified by solution in acetic acid and further precipitation with ether. Further purification may be by dialysis against water or chromatographic adsorption on an aluminium oxide and/or cellulose column.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB43896/59A GB924815A (en) | 1959-12-24 | 1959-12-24 | Improvements in methods of preparation of polypeptides of spasmolytic activity |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB43896/59A GB924815A (en) | 1959-12-24 | 1959-12-24 | Improvements in methods of preparation of polypeptides of spasmolytic activity |
Publications (1)
Publication Number | Publication Date |
---|---|
GB924815A true GB924815A (en) | 1963-05-01 |
Family
ID=10430817
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB43896/59A Expired GB924815A (en) | 1959-12-24 | 1959-12-24 | Improvements in methods of preparation of polypeptides of spasmolytic activity |
Country Status (1)
Country | Link |
---|---|
GB (1) | GB924815A (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2464942A1 (en) * | 1979-09-11 | 1981-03-20 | Novo Industri As | POLYPEPTIDE, METHOD FOR ISOLATION, AND THERAPEUTIC USES |
US4370317A (en) * | 1980-09-10 | 1983-01-25 | Novo Industri A/S | Pancreatic spasmolytic polypeptide |
EP0590403A2 (en) * | 1992-09-28 | 1994-04-06 | NISSEI MARINE IND. Co., Ltd. | Compositions having analgesic action |
-
1959
- 1959-12-24 GB GB43896/59A patent/GB924815A/en not_active Expired
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2464942A1 (en) * | 1979-09-11 | 1981-03-20 | Novo Industri As | POLYPEPTIDE, METHOD FOR ISOLATION, AND THERAPEUTIC USES |
US4370317A (en) * | 1980-09-10 | 1983-01-25 | Novo Industri A/S | Pancreatic spasmolytic polypeptide |
EP0590403A2 (en) * | 1992-09-28 | 1994-04-06 | NISSEI MARINE IND. Co., Ltd. | Compositions having analgesic action |
EP0590403A3 (en) * | 1992-09-28 | 1995-02-22 | Nissei Marine Ind Co Ltd | Compositions having analgesic action. |
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