GB924815A - Improvements in methods of preparation of polypeptides of spasmolytic activity - Google Patents

Improvements in methods of preparation of polypeptides of spasmolytic activity

Info

Publication number
GB924815A
GB924815A GB43896/59A GB4389659A GB924815A GB 924815 A GB924815 A GB 924815A GB 43896/59 A GB43896/59 A GB 43896/59A GB 4389659 A GB4389659 A GB 4389659A GB 924815 A GB924815 A GB 924815A
Authority
GB
United Kingdom
Prior art keywords
broth
polypeptide
polypeptides
alcohol
precipitate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB43896/59A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to GB43896/59A priority Critical patent/GB924815A/en
Publication of GB924815A publication Critical patent/GB924815A/en
Expired legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/26—Lymph; Lymph nodes; Thymus; Spleen; Splenocytes; Thymocytes

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Virology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Polypeptides having spasmolytic activity are obtained from animal organs of lymphoidal structure e.g. lymph ganglions of oxen or sheep or calf or lamb thymus, by hydrolysing the comminuted organs to a broth by warming with an aqueous solution of a protease e.g. papain, heating the broth to destroy the enzyme, clarifying the broth and fractionally precipitating the polypeptide from the clarified broth with alcohol, the active material being recovered from the product precipitated with the greatest concentration of alcohol. The hydrolysis may be carried out at 60 DEG C. for 15 minutes at a pH of 7.0. After filtration, the clarified broth may be treated with an equal volume of 90% ethanol, the precipitate discarded, and seven volumes of absolute ethanol added to precipitate the polypeptide. The crude polypeptide may be purified by solution in acetic acid and further precipitation with ether. Further purification may be by dialysis against water or chromatographic adsorption on an aluminium oxide and/or cellulose column.
GB43896/59A 1959-12-24 1959-12-24 Improvements in methods of preparation of polypeptides of spasmolytic activity Expired GB924815A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB43896/59A GB924815A (en) 1959-12-24 1959-12-24 Improvements in methods of preparation of polypeptides of spasmolytic activity

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB43896/59A GB924815A (en) 1959-12-24 1959-12-24 Improvements in methods of preparation of polypeptides of spasmolytic activity

Publications (1)

Publication Number Publication Date
GB924815A true GB924815A (en) 1963-05-01

Family

ID=10430817

Family Applications (1)

Application Number Title Priority Date Filing Date
GB43896/59A Expired GB924815A (en) 1959-12-24 1959-12-24 Improvements in methods of preparation of polypeptides of spasmolytic activity

Country Status (1)

Country Link
GB (1) GB924815A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2464942A1 (en) * 1979-09-11 1981-03-20 Novo Industri As POLYPEPTIDE, METHOD FOR ISOLATION, AND THERAPEUTIC USES
US4370317A (en) * 1980-09-10 1983-01-25 Novo Industri A/S Pancreatic spasmolytic polypeptide
EP0590403A2 (en) * 1992-09-28 1994-04-06 NISSEI MARINE IND. Co., Ltd. Compositions having analgesic action

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2464942A1 (en) * 1979-09-11 1981-03-20 Novo Industri As POLYPEPTIDE, METHOD FOR ISOLATION, AND THERAPEUTIC USES
US4370317A (en) * 1980-09-10 1983-01-25 Novo Industri A/S Pancreatic spasmolytic polypeptide
EP0590403A2 (en) * 1992-09-28 1994-04-06 NISSEI MARINE IND. Co., Ltd. Compositions having analgesic action
EP0590403A3 (en) * 1992-09-28 1995-02-22 Nissei Marine Ind Co Ltd Compositions having analgesic action.

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