GB866423A - Improvements in or relating to chymotrypsin solutions - Google Patents

Abstract

Chymotrypsin is stabilised in aqueous solution by adding one or more amino acids free from sulphhydryl and disulphide groups, the pH of the solution being less than 7,0 and being preferably 3,5 to 6,0 and especially 3,7 or 4,3. The preferred amino acids are glycine, methionine and valine but others which may be used are threonine, serine, proline, hydroxy-1-proline and tryptophane. It is preferred to use pure alpha chymotrypsin. The concentration of the enzyme is from 1 to 10, and preferably 4 to 6, mg/c.c. The amino acids are used in concentrations of 0,5 to 10, and preferably 4 to 6%. Further stability is obtained by adding a water-soluble non-toxic calcium salt e.g. the acetate, gluconate, chloride, lactate or levulinate in concentrations of 0,05 to 2,0 and especially 0,15 to 0,25%. Preservatives may also be present. The solutions may be used medicinally. Specification 855,163 is referred to.ALSO:A therapeutic composition for topical or parenteral use comprises an aqueous solution of chymotrypsin containing one or more amino acids free from sulphhydryl and disulphide groups, the pH of the solution being less than 7.0 and being preferably 3.5 to 6.0 and especially 3.7 to 4.3. The preferred amino acids are glycine, methionine and valine but others which may be used are threonine, serine, proline, hydroxy-1-proline and trytophane. It is preferred to use pure alpha chymotrypsin. The concentration of the enzyme is from 1 to 10, and preferably 4 to 6, mg./c.c. The amino acids are used in concentrations of 0.5 to 10, and preferably 4 to 6%. Further stability is obtained by adding a water-soluble non-toxic calcium salt, e.g. the acetate, gluconate, chloride, lactate or levulinate in concentrations of 0.05 to 2.0 and especially 0.15 to 0.25%. Preservatives may be present, e.g. phenol. Specification 855,163 is referred to.