GB854918A - Azotobacter concentrates obtained by submerged aerobic fermentation - Google Patents

Azotobacter concentrates obtained by submerged aerobic fermentation

Info

Publication number
GB854918A
GB854918A GB3066/57A GB306657A GB854918A GB 854918 A GB854918 A GB 854918A GB 3066/57 A GB3066/57 A GB 3066/57A GB 306657 A GB306657 A GB 306657A GB 854918 A GB854918 A GB 854918A
Authority
GB
United Kingdom
Prior art keywords
culture
sterile
waste
culture medium
magnesium carbonate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB3066/57A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of GB854918A publication Critical patent/GB854918A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/065—Azotobacter

Abstract

A concentrated azotobacter culture for use in soil bacterizing and fodder preparations is obtained by preparing a sterile culture medium from nutrients and assimilable carbonaceous waste material free or substantially free of nitrogen, diluting same with sterile water, adding thereto sterile magnesium carbonate and a sterile adsorbent, stirring, inoculating with an azotobacter culture with simultaneous aeration, fermenting until substantially all carbonaceous waste material has been utilised and thereafter adding a further quantity of said culture medium, magnesium carbonate and adsorbent, allowing fermentation to proceed at a pH value of 6,5 to 7,0 with aeration until substantially all the carbon source has been utilised and separating, washing and rethickening the mature mash twice to obtain a bacterial concentrate. In the sample, the culture medium contains glucose waste (74,4% glucose, 18,5% dextrin and 0,032% nitrogen in a solids content of 94,8%) KHPO4, K2HPO4, MgSO4, NaCl, CaSO4, sodium molybdate and water and magnesium carbonate and activated carbon is added. In place of the glucose waste, there may be used waste starch, starch refuse, syrups and waste waters containing such materials. Other adsorbents are bone dust, kaolin, Fuller's earth and silica gel. The temperature of cultivation is 25 to 30 DEG C. and the duration 36 to 48 hours. The final culture may be used to inoculate a fresh batch of culture medium. A soil bacterizing preparation is produced by mixing the final culture with sterile humus soil, kaolin or fuller's earth in proportions of 1 to 20 to 1 to 100. In the case of an animal feed, the final culture is mixed with animal fodder.ALSO:A concentrated azotobacter culture for use in soil bacterizing and fodder preparations is obtained by preparing a sterile culture medium from nutrients and assimilable carbonaceous waste material free or substantially free of nitrogen, diluting same with sterile water, adding thereto sterile magnesium carbonate and a sterile adsorbent, stirring, inoculating with an azotobacter culture with simultaneous aeration, fermenting until substantially all carbonaceous waste material has been utilised and thereafter adding a further quantity of said culture medium, magnesium carbonate and adsorbent, allowing fermentation to proceed at a pH value of 6,5 to 7,0 with aeration until substantially all the carbon source has been utilized and separating, washing and re-thickening the mature mash twice to obtain a bacterial concentrate. In the example, the culture medium contains glucose waste (74,4% glucose, 18,5% dextrin and 0,032% nitrogen in a solids content of 94,8%) KHPO4, K2HPO4, MgSO4, NaCl, CaSO4, sodium molybdate and water and magnesium carbonate and activated carbon is added. In place of the glucose waste, there may be used waste starch, starch refuse, syrups and waste waters containing such materials. Other adsorbents are bone dust, kaolin, Fuller's earth and silica gel. The temperature of cultivation is 25 to 30 DEG C. and the duration 36 to 48 hours. The final culture may be used to inoculate a fresh batch of culture medium. A soil bacterizing preparation is produced by mixing the final culture with sterile humus soil, kaolin, or Fuller's earth in proportions of 1 to 20 to 1 to 100. In the case of an animal feed, the final culture is mixed with animal fodder.
GB3066/57A 1956-02-06 1957-01-29 Azotobacter concentrates obtained by submerged aerobic fermentation Expired GB854918A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CS854918X 1956-02-06

Publications (1)

Publication Number Publication Date
GB854918A true GB854918A (en) 1960-11-23

Family

ID=5456188

Family Applications (1)

Application Number Title Priority Date Filing Date
GB3066/57A Expired GB854918A (en) 1956-02-06 1957-01-29 Azotobacter concentrates obtained by submerged aerobic fermentation

Country Status (1)

Country Link
GB (1) GB854918A (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3205060A (en) * 1962-03-26 1965-09-07 Standard Oil Co Soil conditioning composition
FR2352055A1 (en) * 1976-05-18 1977-12-16 Mueller Hans MICROORGANISMS RECYCLING PROCESS
EP0341736A1 (en) * 1988-05-13 1989-11-15 BIOCHEMIE Gesellschaft m.b.H. Fertilizer comprising bacterial biomass, process for its production and its use as a special organic fertilizer
EP0369223A1 (en) * 1988-10-27 1990-05-23 Biochemie Gesellschaft M.B.H. Bacterial biomass fertilizer
WO2018096429A1 (en) * 2016-11-22 2018-05-31 Solyom Zoltan Procedure for preserving soil bacteria, suitably to produce preserved soil-improving bio-fertilizers

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3205060A (en) * 1962-03-26 1965-09-07 Standard Oil Co Soil conditioning composition
FR2352055A1 (en) * 1976-05-18 1977-12-16 Mueller Hans MICROORGANISMS RECYCLING PROCESS
EP0341736A1 (en) * 1988-05-13 1989-11-15 BIOCHEMIE Gesellschaft m.b.H. Fertilizer comprising bacterial biomass, process for its production and its use as a special organic fertilizer
EP0369223A1 (en) * 1988-10-27 1990-05-23 Biochemie Gesellschaft M.B.H. Bacterial biomass fertilizer
WO2018096429A1 (en) * 2016-11-22 2018-05-31 Solyom Zoltan Procedure for preserving soil bacteria, suitably to produce preserved soil-improving bio-fertilizers

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