GB768971A - Antibiotic d-52, its salts and method of preparation - Google Patents
Antibiotic d-52, its salts and method of preparationInfo
- Publication number
- GB768971A GB768971A GB10293/55A GB1029355A GB768971A GB 768971 A GB768971 A GB 768971A GB 10293/55 A GB10293/55 A GB 10293/55A GB 1029355 A GB1029355 A GB 1029355A GB 768971 A GB768971 A GB 768971A
- Authority
- GB
- United Kingdom
- Prior art keywords
- antibiotic
- acid
- penicillin
- solvent
- pict
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/28—Streptomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/465—Streptomyces
Abstract
<PICT:0768971/IV(b)/1> <PICT:0768971/IV(b)/2> <PICT:0768971/IV(b)/3> A new antibiotic, designated antibiotic D-52, is produced by cultivating under aerobic conditions an antibiotic D-52-producing strain of Streptomyces caelestis, especially Streptomyces caelestis NRRL 2418, in a culture medium containing assimilable sources of carbohydrate, nitrogen and inorganic salts. Specified sources of (a) carbohydrates, are brown sugar, lactose, dextrin, starch, sucrose, glucose and molasses; (b) nitrogen, are corn steep liquor, brewers' yeast, distillers' solutes, soyabean meal or flour, casein, amino acid mixtures, peptones, nitrates and ammonium sulphate; (c) inorganic salts, are salts yielding sodium, potassium, calcium, phosphate, chloride and sulphate ions. Essential trace elements also required. Culture may be surface or submerged. The initial pH of the medium is 6.5 to 7.5 and preferably 7.0. The temperature of cultivation 20 DEG to 32 DEG C., and preferably 24 DEG to 28 DEG C., and the duration of cultivation is 2 to 6 days (submerged) or 4 to 8 days (surface). Antibiotic D-52 is isolated from the clarified broth by (a) solvent extraction or (b) adsorption. In the solvent extraction, the broth is adjusted to pH 6 to 10 and preferably 7.5 to 8.0 and extracted with a water-immiscible polar solvent, e.g. chlorinated hydrocarbon such as chloroform, an alcohol such as butanol, an alkyl ester such as ethyl acetate, or a ketone such as methyl isobutyl ketone. The extract is evaporated in vacuo to produce the crude antibiotic. Alternatively, the extract is concentrated and a non-solvent, e.g. hexane, added to precipitate the antibiotic. Adsorbing agents used in the adsorption process are activated carbon especially when pretreated with acetic acid, activated alumina, silica gel, and magnesium aluminium silicate. Elution is by a polar organic solvent for the antibiotic. Antibiotic D-52 has a broad antibacterial spectrum. It is amphoteric, being soluble in water at a pH 1 to 7, insoluble at pH 7.5 to 9.0 and soluble at pH 10 to 13. It is stable at pH 2 to 7. It is soluble in methanol, chloroform, ethyl acetate and methylene chloride but insoluble in ether and ligroin. Empirical formula C23H36-40O9N2S.[d]24D = 121.5 DEG (in chloroform). Ultraviolet absorption in aqueous solution gives a maxima of E1 per cent1 cm.=182 at 239 millimicrons and E1 per cent1 cm.=74 at 307 millimicrons. A suspension in liquid petrolatum exhibits characteristic absorption in infra-red at 3340, 3210, 1900, 1672, 1655, 1570, 1545, 1488, 1090 and 758 cm.-1. Examples describe the preparation of (a) antibiotic D-52 hydrochloride by passing dry hydrochloric acid into a methylene chloride solution of D-52; (b) D-52-oxalate by adding oxalic acid dihydrate to a methanol solution of D-52 and adding ether to precipitate the oxalate; (c) D-52 salicylate by adding salicylic acid to a methanol solution of D-52 and adding ether to precipitate the salicylate. Antibiotic D-52 is incorporated into preparations for the treatment of man, animals and plants (see Group VI).ALSO:A new antibiotic D52 (see Group IV(b)) is used as a feed supplement in promoting the growth of animal and poultry alone or in conjunction with one or more of the following antibiotics: tetracycline, chlortetracycline, oxytetracycline, streptomycin, dihydrostreptomycin, penicillin, chloramphenicol, bacitracin, actidione, circulin, tyrothricin, polymixin B, endomycin, amicetin, fumagillin and erythromycin.ALSO:A new antibiotic D-52 is incorporated into preparations for the treatment of man, animal and plants. In the case of plants, it is used alone or in admixture with one or more of the antibiotics oxytetracycline, chlortetracycline, penicillen, streptomycin or actidione. In the case of man and animals, antibiotic D-52 may be combined with one or more of the following: (a) antibiotics such as streptomycin, dihydrostreptomycin oxytetracycline, tetracycline, chlortetracycline, bacitracin, circulin, penicillins, e.g. penicillin O procaine penicillin and chloroprocaine penicillin, chloramphenicol, tyrothricin, polymixin B, endomycin, amicetin, fumagillin and erythromycin; (b) sulpha compounds such as sulphadiazine, sulphamerazine and sulphamethazine; (c) steroid hormones such as cortisone, hydrocortisone, and esters thereof; halo-cortisones such as 9-a -chlorocortisones, and 9 - a - fluoro-cortisone, and esters thereof; (d) estrogens; (e) analgines, e.g. salicylates; (f) antihistaminies such as pyrrolidine ethyl phenothiazine hydrochloride and N1-pyridyl-N1-benzyl-N-dimethyl ethylene diamine; and (g) antifungal agents such as caprylic acid, undecylenic acid and p-hydroxybenzoic acid.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US768971XA | 1954-04-29 | 1954-04-29 |
Publications (1)
Publication Number | Publication Date |
---|---|
GB768971A true GB768971A (en) | 1957-02-27 |
Family
ID=22134834
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB10293/55A Expired GB768971A (en) | 1954-04-29 | 1955-04-07 | Antibiotic d-52, its salts and method of preparation |
Country Status (1)
Country | Link |
---|---|
GB (1) | GB768971A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4384043A (en) * | 1981-09-02 | 1983-05-17 | American Cyanamid Company | Process for producing the antibiotic nosiheptide |
-
1955
- 1955-04-07 GB GB10293/55A patent/GB768971A/en not_active Expired
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4384043A (en) * | 1981-09-02 | 1983-05-17 | American Cyanamid Company | Process for producing the antibiotic nosiheptide |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
GB715362A (en) | Improvements in or relating to antimicrobial agent | |
US2928844A (en) | Celesticetin, its salts and method of preparation | |
GB768971A (en) | Antibiotic d-52, its salts and method of preparation | |
GB935075A (en) | The antibiotic decoyinine and the manufacture thereof | |
GB815518A (en) | The antibiotic novobiocin derivatives thereof and pharmaceutical compositions containing same | |
US3022311A (en) | Yohimbine and alpha-yohimbine derivatives | |
GB901273A (en) | The new organic compound tylosin, its hydrolysis product desmycosin and methods for preparing same | |
GB875011A (en) | A new antibiotic chalcomycin and method for producing the same | |
GB1049434A (en) | Oxytetracycline | |
GB707332A (en) | Antibiotics and methods for obtaining same | |
GB708159A (en) | A new antibiotic (erythromycin) and a process for preparing same | |
US2985564A (en) | Production of puromycin | |
GB793797A (en) | Improvements in or relating to antibiotics | |
GB783486A (en) | A new antibiotic filipin | |
GB757089A (en) | Improvements in or relating to antibiotics | |
GB729208A (en) | Improvements in or relating to a new antibiotic p.a. 95 and the preparation thereof | |
GB817385A (en) | Process for the production of an antibiotic designated kanamycin | |
GB764198A (en) | The antibiotic vengicide and processes for the preparation of the antibiotics oxytetracycline and vengicide | |
GB770065A (en) | Production of tetracycline by fermentation | |
US3496268A (en) | Umbrinomycin and diumycin | |
GB829327A (en) | Novel antibiotic designated marcomycin and process of making same | |
GB790521A (en) | Improvements in or relating to antibiotics | |
US2916484A (en) | Neomethymycin | |
GB817730A (en) | Fermentation of a tetracycline-producing-streptomyces | |
GB708686A (en) | Antibiotic d-13 and its production |