GB758751A - Improved method of preparing gonadotropins as well as acth from pituitary material and gonadotropins so produced - Google Patents
Improved method of preparing gonadotropins as well as acth from pituitary material and gonadotropins so producedInfo
- Publication number
- GB758751A GB758751A GB3129/54A GB312954A GB758751A GB 758751 A GB758751 A GB 758751A GB 3129/54 A GB3129/54 A GB 3129/54A GB 312954 A GB312954 A GB 312954A GB 758751 A GB758751 A GB 758751A
- Authority
- GB
- United Kingdom
- Prior art keywords
- gonadotropins
- zinc
- acth
- extraction
- per cent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans derived from pro-opiomelanocortin, pro-enkephalin or pro-dynorphin
- A61K38/35—Corticotropin [ACTH]
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The extraction of ACTH and gonadotropins from the same batch of whole pituitary material e.g. hog pituitary material, comprises extracting the ACTH with acidified aqueous acetone, containing 50 to 85 per cent by volume acetone, at a pH below 4, and subjecting the separated insoluble residue to aqueous extraction at a pH from 7,1 to 10,0 to extract the gonadotropins. The gonadotropins may be precipitated by addition of ethanol, or the extract may be adjusted to a pH between 3 and 7 and the gonadotropin-inert proteins precipitated by addition of a water-soluble zinc salt e.g. acetate, chloride or nitrate, to a concentration of 0,01 to 1,0 molar, prior to the precipitation of the gonadotropins as a zinc complex by the addition of an organic precipitating agent e.g. ethanol (10 to 50 per cent by volume), monohydroxyalkanes, or aliphatic ketones having less than 4 carbon atoms. Extraction of the precipitated zinc complex with aqueous phosphate solution e.g. mono- or dibasic alkali metal phosphates, at pH 7 to 9, which may contain an organic precipitating agent (25 to 55 per cent by volume) results in a supernatent liquid, which contains the gonadotropins free from zinc and inert substances, from which the hormone is recovered by precipitation. Alternatively, the zinc can be removed from the gonadotropin preparation by an ion exchange procedure or dialysis in the presence of a sequestering agent. The hormone may be further purified by fractionation with ammonium sulphate at pH 4,5 to 8,0. The process takes place at a temperature of less than 10 DEG C.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US758751XA | 1953-02-19 | 1953-02-19 |
Publications (1)
Publication Number | Publication Date |
---|---|
GB758751A true GB758751A (en) | 1956-10-10 |
Family
ID=22128516
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB3129/54A Expired GB758751A (en) | 1953-02-19 | 1954-02-02 | Improved method of preparing gonadotropins as well as acth from pituitary material and gonadotropins so produced |
Country Status (1)
Country | Link |
---|---|
GB (1) | GB758751A (en) |
-
1954
- 1954-02-02 GB GB3129/54A patent/GB758751A/en not_active Expired
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