GB2605340B - Base editing enzymes - Google Patents

Base editing enzymes Download PDF

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Publication number
GB2605340B
GB2605340B GB2209534.3A GB202209534A GB2605340B GB 2605340 B GB2605340 B GB 2605340B GB 202209534 A GB202209534 A GB 202209534A GB 2605340 B GB2605340 B GB 2605340B
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Prior art keywords
base editing
editing enzymes
enzymes
base
editing
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GB2209534.3A
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GB2605340A8 (en
GB2605340A (en
GB202209534D0 (en
Inventor
Lin Jyun-Liang
Brooks Alan
Butterfield Cristina
Brown Christopher
Castelle Cindy
C Thomas Brian
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Metagenomi Therapeutics Inc
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Metagenomi Inc
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2497—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing N- glycosyl compounds (3.2.2)
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    • C12N9/14—Hydrolases (3)
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    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/02—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2) hydrolysing N-glycosyl compounds (3.2.2)
    • C12Y302/02027—Uracil-DNA glycosylase (3.2.2.27)
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    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
    • C12Y305/04004—Adenosine deaminase (3.5.4.4)
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    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
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    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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GB2209534.3A 2020-09-11 2021-09-10 Base editing enzymes Active GB2605340B (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063077057P 2020-09-11 2020-09-11
US202163222351P 2021-07-15 2021-07-15
PCT/US2021/049962 WO2022056324A1 (en) 2020-09-11 2021-09-10 Base editing enzymes

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GB202209534D0 GB202209534D0 (en) 2022-08-10
GB2605340A GB2605340A (en) 2022-09-28
GB2605340A8 GB2605340A8 (en) 2024-02-21
GB2605340B true GB2605340B (en) 2024-12-18

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US (3) US12286654B2 (https=)
EP (1) EP4211234A4 (https=)
JP (1) JP2023540797A (https=)
KR (1) KR20230062873A (https=)
AU (1) AU2021339819A1 (https=)
CA (1) CA3192224A1 (https=)
GB (1) GB2605340B (https=)
MX (1) MX2023002913A (https=)
WO (1) WO2022056324A1 (https=)

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KR20210055733A (ko) 2018-09-07 2021-05-17 빔 테라퓨틱스, 인크. 핵염기 편집 시스템을 전달하기 위한 조성물 및 방법
EP3850088A4 (en) 2018-09-07 2023-07-19 Beam Therapeutics, Inc. Compositions and methods for improving base editing
WO2020168122A1 (en) 2019-02-13 2020-08-20 Beam Therapeutics Inc. Modified immune cells having adenosine deaminase base editors for modifying a nucleobase in a target sequence
EP4034138A4 (en) 2019-09-27 2024-07-31 Beam Therapeutics, Inc. COMPOSITIONS AND METHODS FOR THE TREATMENT OF LIQUID CANCERS
GB2617658B (en) 2020-03-06 2024-04-17 Metagenomi Inc Class II, type V CRISPR systems
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CA3192224A1 (en) 2020-09-11 2022-03-17 Jyun-Liang LIN Base editing enzymes
AU2021347359A1 (en) 2020-09-25 2023-05-18 Beam Therapeutics Inc. Fratricide resistant modified immune cells and methods of using the same
BR112023014719A2 (pt) * 2021-01-22 2023-12-05 Metagenomi Inc Novas nucleases quiméricas e manipuladas
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EP4211234A1 (en) 2023-07-19
GB2605340A8 (en) 2024-02-21
US20230374482A1 (en) 2023-11-23
GB2605340A (en) 2022-09-28
AU2021339819A1 (en) 2023-05-25
CA3192224A1 (en) 2022-03-17
WO2022056324A1 (en) 2022-03-17
US20220364067A1 (en) 2022-11-17
JP2023540797A (ja) 2023-09-26
MX2023002913A (es) 2023-06-01
EP4211234A4 (en) 2025-04-30
KR20230062873A (ko) 2023-05-09
US20230348876A1 (en) 2023-11-02
GB202209534D0 (en) 2022-08-10
US12286654B2 (en) 2025-04-29

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