GB2600372A - Fluorescent Cytosine analogues and their application in transcription and translation - Google Patents

Fluorescent Cytosine analogues and their application in transcription and translation Download PDF

Info

Publication number
GB2600372A
GB2600372A GB2009705.1A GB202009705A GB2600372A GB 2600372 A GB2600372 A GB 2600372A GB 202009705 A GB202009705 A GB 202009705A GB 2600372 A GB2600372 A GB 2600372A
Authority
GB
United Kingdom
Prior art keywords
compound
formula
salt
labelled
rna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB2009705.1A
Other languages
English (en)
Other versions
GB202009705D0 (en
Inventor
Wilhelmsson Marcus
Esbjörner Elin
Baladi Tom
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chalmers Ventures AB
Original Assignee
Chalmers Ventures AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chalmers Ventures AB filed Critical Chalmers Ventures AB
Priority to GB2009705.1A priority Critical patent/GB2600372A/en
Publication of GB202009705D0 publication Critical patent/GB202009705D0/en
Priority to EP21734353.2A priority patent/EP4172170B1/en
Priority to ES21734353T priority patent/ES2995470T3/es
Priority to PCT/EP2021/067342 priority patent/WO2021260107A1/en
Priority to IL299302A priority patent/IL299302A/en
Priority to US18/001,805 priority patent/US20230219995A1/en
Priority to DK21734353.2T priority patent/DK4172170T3/da
Priority to JP2022580419A priority patent/JP2023533484A/ja
Publication of GB2600372A publication Critical patent/GB2600372A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/24—Heterocyclic radicals containing oxygen or sulfur as ring hetero atom
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/33—Chemical structure of the base
    • C12N2310/334—Modified C
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/119—RNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/101—Modifications characterised by incorporating non-naturally occurring nucleotides, e.g. inosine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/117—Modifications characterised by incorporating modified base
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Saccharide Compounds (AREA)
  • Electroluminescent Light Sources (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
GB2009705.1A 2020-06-25 2020-06-25 Fluorescent Cytosine analogues and their application in transcription and translation Withdrawn GB2600372A (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
GB2009705.1A GB2600372A (en) 2020-06-25 2020-06-25 Fluorescent Cytosine analogues and their application in transcription and translation
EP21734353.2A EP4172170B1 (en) 2020-06-25 2021-06-24 Fluorescent cytosine analogues and their application in transcription and translation
ES21734353T ES2995470T3 (en) 2020-06-25 2021-06-24 Fluorescent cytosine analogues and their application in transcription and translation
PCT/EP2021/067342 WO2021260107A1 (en) 2020-06-25 2021-06-24 Fluorescent cytosine analogues and their application in transcription and translation
IL299302A IL299302A (en) 2020-06-25 2021-06-24 Fluorescent cytosine analogues and their application in transcription and translation
US18/001,805 US20230219995A1 (en) 2020-06-25 2021-06-24 Fluorescent cytosine analogues and their application in transcription and translation
DK21734353.2T DK4172170T3 (da) 2020-06-25 2021-06-24 Fluorescerende cytosinanaloger og anvendelse deraf i transskription og translation
JP2022580419A JP2023533484A (ja) 2020-06-25 2021-06-24 蛍光シトシンアナログならびに転写および翻訳へのその適用

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB2009705.1A GB2600372A (en) 2020-06-25 2020-06-25 Fluorescent Cytosine analogues and their application in transcription and translation

Publications (2)

Publication Number Publication Date
GB202009705D0 GB202009705D0 (en) 2020-08-12
GB2600372A true GB2600372A (en) 2022-05-04

Family

ID=71949604

Family Applications (1)

Application Number Title Priority Date Filing Date
GB2009705.1A Withdrawn GB2600372A (en) 2020-06-25 2020-06-25 Fluorescent Cytosine analogues and their application in transcription and translation

Country Status (8)

Country Link
US (1) US20230219995A1 (https=)
EP (1) EP4172170B1 (https=)
JP (1) JP2023533484A (https=)
DK (1) DK4172170T3 (https=)
ES (1) ES2995470T3 (https=)
GB (1) GB2600372A (https=)
IL (1) IL299302A (https=)
WO (1) WO2021260107A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025091026A1 (en) * 2023-10-27 2025-05-01 University Of Connecticut Therapeutic compositions containing g-clamp pna and uses thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6028183A (en) * 1997-11-07 2000-02-22 Gilead Sciences, Inc. Pyrimidine derivatives and oligonucleotides containing same
US20030158403A1 (en) * 2001-07-03 2003-08-21 Isis Pharmaceuticals, Inc. Nuclease resistant chimeric oligonucleotides
WO2005020885A2 (en) * 2003-05-21 2005-03-10 Isis Pharmaceuticals, Inc. Compositions and methods for the treatment of severe acute respiratory syndrome (sars)
WO2009120878A2 (en) * 2008-03-26 2009-10-01 Alnylam Pharmaceuticals, Inc. Non-natural ribonucleotides, and methods of use thereof
WO2018089582A1 (en) * 2016-11-10 2018-05-17 San Diego State University Research Foundation Compounds for fluorescence sensing of duplex formation

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011034895A1 (en) * 2009-09-15 2011-03-24 Regents Of The University Of Colorado Compositions, methods and uses for nucleotide analogues

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6028183A (en) * 1997-11-07 2000-02-22 Gilead Sciences, Inc. Pyrimidine derivatives and oligonucleotides containing same
US20030158403A1 (en) * 2001-07-03 2003-08-21 Isis Pharmaceuticals, Inc. Nuclease resistant chimeric oligonucleotides
WO2005020885A2 (en) * 2003-05-21 2005-03-10 Isis Pharmaceuticals, Inc. Compositions and methods for the treatment of severe acute respiratory syndrome (sars)
WO2009120878A2 (en) * 2008-03-26 2009-10-01 Alnylam Pharmaceuticals, Inc. Non-natural ribonucleotides, and methods of use thereof
WO2018089582A1 (en) * 2016-11-10 2018-05-17 San Diego State University Research Foundation Compounds for fluorescence sensing of duplex formation

Also Published As

Publication number Publication date
DK4172170T3 (da) 2024-12-02
WO2021260107A1 (en) 2021-12-30
EP4172170B1 (en) 2024-09-25
JP2023533484A (ja) 2023-08-03
IL299302A (en) 2023-02-01
EP4172170A1 (en) 2023-05-03
US20230219995A1 (en) 2023-07-13
GB202009705D0 (en) 2020-08-12
ES2995470T3 (en) 2025-02-10

Similar Documents

Publication Publication Date Title
Depmeier et al. Strategies for covalent labeling of long RNAs
CN113195720B (zh) 酶促rna合成
US7745417B2 (en) Nucleosides or nucleotides having novel unnatural bases and use thereof
CN109071589A (zh) 5′-硫代磷酸酯mRNA 5′-端(帽)类似物、包含所述类似物的mRNA、其获得方法和用途
Mayer The chemical biology of nucleic acids
Sabat et al. Template-dependent DNA ligation for the synthesis of modified oligonucleotides
Warminski et al. The synthesis of isopropylidene mRNA cap analogs modified with phosphorothioate moiety and their evaluation as promoters of mRNA translation
US20160237439A1 (en) Modular rna regulators and methods
EP4172170B1 (en) Fluorescent cytosine analogues and their application in transcription and translation
US20130253179A1 (en) Functionalization Processes and Reactants Used in Such Processes Using an Isatoic Anhydride or a Derivative Thereof, Biological Molecules Thus Treated and Kits
Yamaguchi et al. Cycloalkane Incorporation Into the 2′, 4′-Bridge of Locked Nucleic Acid: Enhancing Nuclease Stability, Reducing Phosphorothioate Modifications, and Lowering Hepatotoxicity in Antisense Oligonucleotides
Islam et al. Synthesis and biophysical properties of 5′-thio-2′, 4′-BNA/LNA oligonucleotide
Tam et al. Versatility of threose nucleic acids: synthesis, properties, and applications in chemical biology and biomedical advancements
AU2012254580B2 (en) Process for preparing phosphate compound bearing isotope
KR20230157386A (ko) N-(2-아미노에틸)모르폴린-기반 rna 유사체, 이의 제조 방법 및 용도
Höbartner et al. Chemical synthesis of modified RNA
EP3187584B1 (en) Method for non-enzymatic combination of nucleic acid chains
GB2605952A (en) Fluorescent nucleoside triphosphates
Wu Synthetic Nucleic Acid Capable of Post-Polymerization Functionalization and Evolution
WO2023210767A1 (ja) オリゴヌクレオチドの化学的連結反応
Zhang Control the Function of RNA by Site-Specific Bioconjugation
Krömer Modified nucleotides and DNA for electrochemical labelling and defined display of small molecules
Kosmidis Development of site-specific RNA labeling strategies to probe alternative RNA splicing
RU2830607C2 (ru) Композиции олигонуклеотидов и способы с ними
Lyon A mutation in T7 RNA polymerase enhances the yield of 5'-guanosine-analog-initiated RNAs

Legal Events

Date Code Title Description
732E Amendments to the register in respect of changes of name or changes affecting rights (sect. 32/1977)

Free format text: REGISTERED BETWEEN 20220922 AND 20220928

WAP Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1)