GB2445706A - Reprogramming and genetic modification of cells - Google Patents

Reprogramming and genetic modification of cells Download PDF

Info

Publication number
GB2445706A
GB2445706A GB0808244A GB0808244A GB2445706A GB 2445706 A GB2445706 A GB 2445706A GB 0808244 A GB0808244 A GB 0808244A GB 0808244 A GB0808244 A GB 0808244A GB 2445706 A GB2445706 A GB 2445706A
Authority
GB
United Kingdom
Prior art keywords
cell
cells
reprogramming
genetic modification
fusing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB0808244A
Other languages
English (en)
Other versions
GB0808244D0 (en
Inventor
Ian Chambers
Jose Rebero Da Silva
Austin Gerard Smith
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Edinburgh
Original Assignee
University of Edinburgh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0523039A external-priority patent/GB0523039D0/en
Priority claimed from GB0605998A external-priority patent/GB0605998D0/en
Application filed by University of Edinburgh filed Critical University of Edinburgh
Publication of GB0808244D0 publication Critical patent/GB0808244D0/en
Publication of GB2445706A publication Critical patent/GB2445706A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/605—Nanog
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Developmental Biology & Embryology (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
GB0808244A 2005-11-11 2006-11-13 Reprogramming and genetic modification of cells Withdrawn GB2445706A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0523039A GB0523039D0 (en) 2005-11-11 2005-11-11 Reprogramming and genetic modification of cells
GB0605998A GB0605998D0 (en) 2006-03-24 2006-03-24 Reprogramming And Genetic Modification Of Cells
PCT/GB2006/004218 WO2007054720A1 (fr) 2005-11-11 2006-11-13 Reprogrammation et modification genetique de cellules

Publications (2)

Publication Number Publication Date
GB0808244D0 GB0808244D0 (en) 2008-06-11
GB2445706A true GB2445706A (en) 2008-07-16

Family

ID=37763882

Family Applications (2)

Application Number Title Priority Date Filing Date
GB0808244A Withdrawn GB2445706A (en) 2005-11-11 2006-11-13 Reprogramming and genetic modification of cells
GB0702261A Withdrawn GB2434155A (en) 2005-11-11 2007-02-06 Reprogramming and genetic modification of cells

Family Applications After (1)

Application Number Title Priority Date Filing Date
GB0702261A Withdrawn GB2434155A (en) 2005-11-11 2007-02-06 Reprogramming and genetic modification of cells

Country Status (6)

Country Link
EP (1) EP1957643A2 (fr)
JP (1) JP2009515515A (fr)
AU (1) AU2006313518A1 (fr)
GB (2) GB2445706A (fr)
TW (1) TW200730623A (fr)
WO (1) WO2007054720A1 (fr)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8129187B2 (en) 2005-12-13 2012-03-06 Kyoto University Somatic cell reprogramming by retroviral vectors encoding Oct3/4. Klf4, c-Myc and Sox2
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
EP2208786B1 (fr) 2005-12-13 2018-08-01 Kyoto University Facteur de reprogrammation nucléaire
GB0701062D0 (en) * 2007-01-19 2007-02-28 Evocell Ltd Biological materials and uses thereof
CN101802172A (zh) * 2007-05-30 2010-08-11 通用医疗公司 由体细胞产生多能细胞的方法
US9213999B2 (en) 2007-06-15 2015-12-15 Kyoto University Providing iPSCs to a customer
JP2008307007A (ja) 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
EP2090649A1 (fr) 2008-02-13 2009-08-19 Fondazione Telethon Procédé de reprogrammation de cellules différentiées
AU2015201026B2 (en) * 2008-03-17 2017-03-16 The Scripps Research Institute Combined chemical and genetic approaches for generation of induced pluripotent stem cells
CA2718904C (fr) * 2008-03-17 2017-01-03 The Scripps Research Institute Approches chimiques et genetiques combinees pour la generation de cellules souches pluripotentes induites
CA2695522C (fr) 2008-05-02 2019-01-15 Kyoto University Procede de reprogrammation nucleaire
US10047346B2 (en) 2008-08-08 2018-08-14 Mayo Foundation For Medical Education And Research Method of treating heart tissue using induced pluripotent stem cells
JP5645197B2 (ja) * 2009-06-23 2014-12-24 学校法人日本大学 幹細胞の未分化状態を維持する新規方法
US20130029416A1 (en) 2011-07-22 2013-01-31 Tayaramma Thatava Differentiating induced pluripotent stem cells into glucose-responsive, insulin-secreting progeny
US10718022B2 (en) 2015-04-15 2020-07-21 University Of Massachusetts Compositions and methods for XI chromosome reactivation

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol. 313, 2004, U Yoshida-Koide et al, "Involvement of Ras in extraembryonic endoderm differentiation of embryonic stem cells.", pages 475-481 *
CELL, Vol. 113, 2003, I Chambers et al, "Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells" pages 643-655 *
CLONING AND STEM CELLS, vol. 6, 2004, I Chambers, "The molecular basis of pluripotency in mouse embryonic stem cells" pages 386-391 *
GASTROENTEROLOGY, Vol. 126, Suppl. 2, 2004, X-L Kuai et al, "Treatment of surgically induced acute liver failure by transplantation of HNF4-overexpression embryonic stem cells" page A753, *
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, Vol. 7, 2003, S Constantinescu, "Stemness, fusion and renewal of hematopoietic and embryonic stem cells." pages 103-112 *
MECHANISMS OF DEVELOPMENT, Vol. 122, 2005, S-Y Hatano et al, "Pluripotential competence of cells associated with Nanog activity" pages 67-79 *
NATURE, Vol. 441, 2006 J Silva et al, "Nanog promotes transfer of pluripotency after cell fusion", pages 997-1001 *
REPRODUCTION FERTILITY AND DEVELOPMENT, Vol. 17, 2005, T Do Jeong et al, "Comparison of neurosphere cells with cumulus cells after fusion with embryonic stem cells: reprogramming potential" , pages 143-149 *
SCIENCE, Vol. 309, 2005, C Cowan et al, "Nuclear reprogramming of somatic cells after fusion with human embryonic stem cells.", pages 1369-1373 *

Also Published As

Publication number Publication date
TW200730623A (en) 2007-08-16
AU2006313518A2 (en) 2008-06-26
WO2007054720A8 (fr) 2008-11-13
GB0702261D0 (en) 2007-03-14
AU2006313518A1 (en) 2007-05-18
JP2009515515A (ja) 2009-04-16
GB0808244D0 (en) 2008-06-11
GB2434155A (en) 2007-07-18
WO2007054720A1 (fr) 2007-05-18
EP1957643A2 (fr) 2008-08-20

Similar Documents

Publication Publication Date Title
GB2445706A (en) Reprogramming and genetic modification of cells
JP2017148079A5 (fr)
WO2003091431A8 (fr) Methylotrophe produisant une chaine de sucre de type mammifere
WO2013013105A3 (fr) Compositions et procédés de reprogrammation de cellules sans modification génétique pour la réparation de cartilages endommagés
IL181437A0 (en) Medium and culture of embryonic stem cells
EA200501774A1 (ru) Культуры e1-иммортализованных клеток и способы их культивирования с целью повышения выхода их продукции
BR0012099A (pt) Transferência citoplásmica para de-diferenciar células recipientes
AU2002311989A8 (en) Methods and materials for the production of organic products in cells of $i(candida) species
WO2002072762A3 (fr) Utilisation d'interference de l'arn pour produire des cellules souches de lignee specifique et d'autres cellules non differenciees, et production de cellules differenciees in vitro par coculture
MX2007003906A (es) Metodos y composiciones para mejorar la produccion de proteinas recombinantes.
GB2429211A (en) Feeder independent extended culture of embryonic stem cells
WO2008020333A3 (fr) Acide nucléique codant la n-méthylputrescine oxydase et ses utilisations
WO2012052854A3 (fr) Lignées cellulaires végétales et procédés pour les isoler
BRPI9916095B8 (pt) célula e linhagem de célula expressando uma proteína heteróloga
WO2012007965A8 (fr) Enzyme pour la production de 3-quinuclidinol optiquement pur
Kim et al. Encapsulation of bone morphogenic protein-2 with Cbfa1-overexpressing osteogenic cells derived from human embryonic stem cells in hydrogel accelerates bone tissue regeneration
EP1200557A4 (fr) Procede de fabrication de chromosomes artificiels de plantes
PL326087A1 (en) Improved integration of exogenic dna introduced into eucariotic cells
Laht et al. Cloning and characterization of glucokinase from a methylotrophic yeast Hansenula polymorpha: different effects on glucose repression in H. polymorpha and Saccharomyces cerevisiae
Ozawa et al. Culturing human pluripotent stem cells for regenerative medicine
Walker et al. Analyses of in vivo interactions between transcription factors and the archaeal RNA polymerase
WO2007056062A3 (fr) Procedes pour adapter des cellules de mammifere
Oncel et al. Genetic optimization of microalgae for biohydrogen production
WO2005054447A3 (fr) Cellules souches de muller
Kose et al. Biohydrogen production from microalgae: An enzyme perspective

Legal Events

Date Code Title Description
WAP Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1)