GB2429209A - A cost-effective process for preparing agarose from gracilaria spp - Google Patents
A cost-effective process for preparing agarose from gracilaria spp Download PDFInfo
- Publication number
- GB2429209A GB2429209A GB0624561A GB0624561A GB2429209A GB 2429209 A GB2429209 A GB 2429209A GB 0624561 A GB0624561 A GB 0624561A GB 0624561 A GB0624561 A GB 0624561A GB 2429209 A GB2429209 A GB 2429209A
- Authority
- GB
- United Kingdom
- Prior art keywords
- agarose
- extractive
- mass
- cost
- gracilaria
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 241000206581 Gracilaria Species 0.000 title abstract description 4
- 229920000936 Agarose Polymers 0.000 title abstract 4
- 238000004519 manufacturing process Methods 0.000 title 1
- 241001474374 Blennius Species 0.000 abstract 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 abstract 2
- 239000007788 liquid Substances 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 2
- 241000873911 Gracilaria dura Species 0.000 abstract 1
- 239000003513 alkali Substances 0.000 abstract 1
- 239000003610 charcoal Substances 0.000 abstract 1
- 239000000706 filtrate Substances 0.000 abstract 1
- 238000001914 filtration Methods 0.000 abstract 1
- 230000008014 freezing Effects 0.000 abstract 1
- 238000007710 freezing Methods 0.000 abstract 1
- 238000010438 heat treatment Methods 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
- 239000000047 product Substances 0.000 abstract 1
- 238000010257 thawing Methods 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
- 229920001817 Agar Polymers 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 241001180358 Gracilaria eucheumoides Species 0.000 description 1
- 241001428219 Gracilariaceae Species 0.000 description 1
- 241000206572 Rhodophyta Species 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0036—Galactans; Derivatives thereof
- C08B37/0039—Agar; Agarose, i.e. D-galactose, 3,6-anhydro-D-galactose, methylated, sulfated, e.g. from the red algae Gelidium and Gracilaria; Agaropectin; Derivatives thereof, e.g. Sepharose, i.e. crosslinked agarose
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/10—Process efficiency
- Y02P20/133—Renewable energy sources, e.g. sunlight
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Materials Engineering (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Edible Seaweed (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The present invention relates to a simple, direct and cost-effective process for the preparation of agarose of high gel strength and low gelling temperature from naturally occurring or cultivated Gracilaria spp. more particularly Gracilaria dura, said process comprising steps of pre-treating the dry seaweed with alkali, rinsing the pre-treated seaweed until the washing shows a pH ranging between 7 and 8, adding water, autoclaving to obtain extractive, treating the extractive with charcoal and Celite to obtain hot extractive, vacuum-filtering the hot extractive over a Celite bed, freezing the filtrate into a mass and thawing the mass, redissolving the mass in water by heating in an autoclave, repeating the freeze- thaw cycle, straining the product to remove thawed liquid and thereafter squeezing to expel residual liquid to the extent possible to obtain agarose, and an agarose thereof.
Description
GB 2429209 A continuation (72) cont (56) cont Bharatkumar Kalidas Ramavat
Botanica Marina, Vol. 40, No. 5, September Pushpito Kumar Ghosh 1997, R. D. Villanueva et al, "Optimized agar Karuppanan Eswaran extraction from Gracilaria eucheumoides Sangalya Thiruppathi Harvey", pages 369-372.
Vaibhav Ajit Mantri Journal of Biotechnology, Vol. 89, No. 1, 26 July 2001, E. Marinho-Soriano, "Agar (74) Agent and/or Address for Service: polysaccharides from Gracilaria species Lloyd Wise (Rhodophyta, Gracilariaceae)", pages 81-84.
Commonwealth House, Journal of Applied Phycology, Vol. 7, No. 3, 1-19 New Oxford Street, LONDON, R. Armisen, "World-wide use and WC1A 1LW, United Kingdom importance of Gracilaria", pages 231 -243.
(58) Field of Search by ISA:
INT CL CO8B, C12P Other: EPO-Internal, BIOSIS, WPI Data
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/IB2004/001792 WO2005118830A1 (en) | 2004-06-03 | 2004-06-03 | A cost-effective process for preparing agarose from gracilaria spp. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| GB0624561D0 GB0624561D0 (en) | 2007-01-17 |
| GB2429209A true GB2429209A (en) | 2007-02-21 |
Family
ID=34957898
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB0624561A Withdrawn GB2429209A (en) | 2004-06-03 | 2004-06-03 | A cost-effective process for preparing agarose from gracilaria spp |
Country Status (7)
| Country | Link |
|---|---|
| JP (1) | JP2008501816A (en) |
| CN (1) | CN1993474A (en) |
| AU (1) | AU2004320343A1 (en) |
| CA (1) | CA2569495A1 (en) |
| GB (1) | GB2429209A (en) |
| RU (1) | RU2381276C2 (en) |
| WO (1) | WO2005118830A1 (en) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101138414B (en) * | 2007-10-22 | 2011-11-30 | 天津科技大学 | Method for extracting phycoerythrin and gelose synchronously from gum-contained varek such as gardon asparagus |
| EP2411418B1 (en) * | 2009-03-24 | 2018-09-12 | Council of Scientific & Industrial Research | Process for the preparation of agarose polymer from seaweed extractive |
| CN101891835B (en) * | 2010-07-26 | 2012-08-22 | 华侨大学 | Method for separating and preparing agarose from agar by using polyethylene glycol precipitation method |
| CN102775524A (en) * | 2011-05-12 | 2012-11-14 | 高金华 | Method for directly producing agarose from alga |
| CN102775815B (en) * | 2012-08-06 | 2014-03-26 | 青岛德慧海洋生物科技有限公司 | Preparation method of gel additives with controllable slow release |
| CN102964469B (en) * | 2012-10-08 | 2014-12-10 | 福建省绿麒食品胶体有限公司 | Ultrasound-assisted preparation method of high strength Gracilaria agar |
| KR101875115B1 (en) * | 2017-06-30 | 2018-07-10 | 농업회사법인 주식회사 오션푸드코리아 | Manufacturing method for hydrogel foam using agarose form seaweed |
| CN109054053B (en) * | 2018-06-28 | 2020-12-25 | 福建天源兴达生物科技有限公司 | Processing technology of agar with high gel strength |
| EP3880001A1 (en) * | 2018-11-16 | 2021-09-22 | Sea6 Energy Private Ltd. | Plant based gelling fibre, method and application thereof |
| CN109503729A (en) * | 2018-12-12 | 2019-03-22 | 湖南医药学院 | A method of extracting polysaccharide |
| CN112175110B (en) * | 2020-09-18 | 2022-10-04 | 福建省绿麒食品胶体有限公司 | Preparation method of agar glue solution |
| JP7333980B2 (en) * | 2021-03-31 | 2023-08-28 | 伊那食品工業株式会社 | Low strength highly viscous agar |
| JP7350271B1 (en) | 2022-08-10 | 2023-09-26 | 伊那食品工業株式会社 | Agar-containing foods and quality improvers |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3901873A (en) * | 1972-02-14 | 1975-08-26 | Meiji Seika Kaisha | Process for the production of agar from a red alga |
-
2004
- 2004-06-03 WO PCT/IB2004/001792 patent/WO2005118830A1/en not_active Application Discontinuation
- 2004-06-03 JP JP2007514150A patent/JP2008501816A/en not_active Withdrawn
- 2004-06-03 CA CA002569495A patent/CA2569495A1/en not_active Abandoned
- 2004-06-03 RU RU2006146982/13A patent/RU2381276C2/en not_active IP Right Cessation
- 2004-06-03 AU AU2004320343A patent/AU2004320343A1/en not_active Abandoned
- 2004-06-03 CN CNA200480043673XA patent/CN1993474A/en active Pending
- 2004-06-03 GB GB0624561A patent/GB2429209A/en not_active Withdrawn
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3901873A (en) * | 1972-02-14 | 1975-08-26 | Meiji Seika Kaisha | Process for the production of agar from a red alga |
Non-Patent Citations (7)
| Title |
|---|
| Biochemikalien und Reagenzien, 2002, Sigma-Aldrich, Taufkirchen, pages 53-54. * |
| Botanica Marina, Vol. 40, No. 5, September 1997, R. D. Villanueva et al, "Optimized agar extraction from Gracilaria eucheumoides Harvey", pages 369-372. * |
| Fluka Laboratory Chemicals, 2001, Sigma-Aldrich, Seelze, pages 94-97. * |
| Journal of Applied Phycology, Vol. 7, No. 3, R. Armisen, "World-wide use and importance of Gracilaria", pages 231-243. * |
| Journal of Applied Phycology, Vol. 8, No. 6, 1996, J. Rebello et al, "Agar quality of commercial agarophytes from different geographical origins: 1. Physical and rheological properties", pages 517-521. * |
| Journal of Applied Phycology, Vol. 9, No. 6, 1997, Y. Freile-Pelegrin et al, "Influence of alkali treatment on agar from Gracilaria cornea from Yucatan, Mexico", pages 533-539. * |
| Journal of Biotechnology, Vol. 89, No. 1, 26 July 2001, E. Marinho-Soriano, "Agar polysaccharides from Gracilaria species (Rhodophyta, Gracilariaceae)", pages 81-84. * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2005118830A1 (en) | 2005-12-15 |
| JP2008501816A (en) | 2008-01-24 |
| CA2569495A1 (en) | 2005-12-15 |
| RU2381276C2 (en) | 2010-02-10 |
| GB0624561D0 (en) | 2007-01-17 |
| AU2004320343A1 (en) | 2005-12-15 |
| RU2006146982A (en) | 2008-07-20 |
| CN1993474A (en) | 2007-07-04 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| WAP | Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1) |