CN113748784A - Method for promoting rhus chinensis seeds to germinate rapidly - Google Patents
Method for promoting rhus chinensis seeds to germinate rapidly Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses a method for promoting rhus chinensis seeds to germinate rapidly, which belongs to the technical field of germination promotion, and comprises the steps of screening rhus chinensis seeds with qualified quality, sterilizing the seeds, soaking the seeds in water, soaking the seeds in gibberellin, accelerating germination of the seeds and the like, wherein potassium permanganate solution is adopted to sterilize the rhus chinensis seeds with qualified quality detection, remove mould on the surfaces of the seeds, hot water and warm water are adopted to soak the seeds, so that the dormant mechanism of the rhus chinensis seeds in the Anacardiaceae family is broken through, and then the gibberellin solution is used to soak the treated rhus chinensis seeds, so that the degradation of macromolecular substances is effectively promoted, meanwhile, the activity of hydrolase is improved, and the smooth proceeding of the synthetic action in the seed germination process is facilitated; the method for promoting the rhus chinensis seeds to germinate rapidly provided by the invention can enable the germination rate and the survival rate of the rhus chinensis germinated seeds to reach 100% basically, is simple to operate, has high germination rate and greatly shortens the germination time, and is suitable for popularization and application.
Description
Technical Field
The invention relates to the technical field of germination promotion, in particular to a method for promoting rapid germination of rhus chinensis seeds.
Background
Rhus chinensis, a tree of the genus Rhus of the family Anacardiaceae, is an arbor that can grow to 8 m. The tree has wide spherical crown, compound leaves with odd feathers and bright red leaves in autumn and winter, and is a colorful-leaf tree with great ornamental value. Can be used as garden tree, street tree and shade tree. The rhus chinensis is extremely strong in adaptability, deep in root, strong in tillering property and fast in growth, and can be developed and utilized as a countryside slope protection plant for ecological restoration of a bare slope. In addition, the rhus chinensis is an important economic tree species, branches and leaves of the rhus chinensis are mainly parasitic by gallnut aphids to form gallnut, the rhus chinensis is called gallnut and is clinically used for relieving cough and arresting sweating, astringing to arrest bleeding and relieving diarrhea with astringents, and the like, and the rhus chinensis has important medical value. The oil content of the rhus chinensis seeds is high, and the oil can be extracted to prepare soap and can also be used in food industry; the root, leaf, flower, fruit and gallnut can be used as medicine, and are also good honey source plants. However, the rhus chinensis seeds have hard seed coats and more grease on the surfaces, the hard seed rate is as high as 90.2%, and untreated seeds are difficult to germinate and have uneven seedlings.
The method for removing the limitation of the rhus chinensis seed coat and breaking dormancy mainly comprises a lamination method, a hot water seed soaking method and a sulfuric acid etching method. Compared with chemical seed soaking methods such as acid treatment and the like, the hot water seed soaking method can better soften hard seed coats and increase the water permeability of the seed coats. The hot water method has little pollution, the waste liquid does not need special treatment, is more convenient and environment-friendly, and has lower cost. However, different types of seeds have different hardness and thickness of the skin, and hot water seed soaking has certain limitations. Therefore, the invention provides a method for promoting the rapid germination of rhus chinensis seeds.
Disclosure of Invention
The invention provides a method for promoting rhus chinensis seeds to germinate rapidly, effectively removes the limitation of the seed coats of rhus chinensis, breaks through the limitation of seed soaking in hot water, solves the problems of low germination rate and long germination time of rhus chinensis seeds, and provides a method for promoting rhus chinensis seeds to germinate rapidly with better effect.
The invention provides a method for promoting rhus chinensis seeds to germinate rapidly, which is characterized by comprising the following steps:
s1, seed screening
Selecting qualified rhus chinensis seeds;
s2, Disinfection of seeds
Soaking the qualified seeds in the S1 in a potassium permanganate solution, taking out and cleaning to obtain sterilized seeds;
s3, soaking the seeds in water
Soaking the sterilized seeds of S2 in hot water to obtain hot-dipped seeds; soaking the hot-dipped seeds in water at room temperature again to obtain water-soaked seeds;
s4, soaking the seeds with gibberellin
Soaking the water soaked seeds obtained in the step S3 in gibberellin solution to obtain medicine soaked seeds;
s5, accelerating germination of seeds
And (4) placing the soaked seeds obtained in the step (S4) in an environment with the temperature of 20-30 ℃ and the humidity of 60-70% for accelerating germination.
Preferably, in S1, the quality-qualified rhus chinensis seeds have a purity of 92.1-100%, a thousand-grain weight of 10.09-10.79 g, a hard-seed rate of 90-94%, a water content of 0.031-0.040% and a seed viability of 90-100%.
Preferably, in S2, the mass concentration of the potassium permanganate solution is 1% -5%, and the soaking time is 3-10 min.
Preferably, in S2, the feed-liquid ratio of the qualified seeds to the potassium permanganate solution is 1-5 g:100 ml.
Preferably, in S3 and S3, the temperature of the hot water is 80 ℃, and the soaking time is 20-30 min.
Preferably, in S3, the feed-liquid ratio of the sterilized seeds to the hot water is 1-1.1 g:10 ml; the feed-liquid ratio of the hot-dipped seeds to the warm water is 1-1.1 g:10 ml.
Preferably, in S4, the concentration of the gibberellin solution is 50-55 mg/L, and the soaking time is 24 h.
Preferably, in S4, the feed-liquid ratio of the water-soaked seeds to the gibberellin solution is 1-1.1 g:10 ml.
Compared with the prior art, the invention has the beneficial effects that:
(1) according to the method for promoting rapid germination of rhus chinensis seeds, potassium permanganate solution is adopted to sterilize rhus chinensis seeds qualified in quality detection, mould on the surfaces of the seeds is removed, a dormant mechanism of the rhus chinensis seeds in the family of Anacardiaceae is broken through soaking the seeds with hot water and room temperature water, gibberellin solution is then used to soak the treated rhus chinensis seeds, the skins of the rhus chinensis seeds are effectively softened, degradation of surface grease is promoted, meanwhile, hydrolytic enzyme is induced to generate, macromolecular substances in the seeds, namely starch and protein are promoted to be hydrolyzed into sugar, amino acid and the like, germination of embryos is facilitated, and smooth germination of the seeds is promoted.
(2) The method for promoting the rhus chinensis seeds to germinate rapidly provided by the invention can ensure that the germination rate and the survival rate of the rhus chinensis germinated seeds can reach 100 percent basically.
(3) The method for promoting the rhus chinensis seeds to germinate rapidly provided by the invention is simple to operate, high in germination rate and greatly shortened in germination time, and is suitable for popularization and application.
Drawings
FIG. 1 is a diagram of the germination process of Rhus chinensis seeds; a is a diagram of germination on day 1; b is a diagram of germination on day 3; c is a diagram of germination on day 4; d is a diagram of germination on day 5; e is a diagram of germination on day 6; f is a diagram of germination on day 8;
FIG. 2 is a diagram of the growth process of seedlings of Rhus chinensis; a is a picture of the growth condition of 2 days of seedlings; b is a growth condition chart of 6 days of seedlings; c, a picture is a picture of the growth condition of 7 days of seedlings; d, a picture is a picture of the growth condition of 15 days of seedlings; e picture is a picture of 28-day growth of seedlings; f is the growth condition of the seedling for 30 days.
Detailed Description
In order to make the technical solutions of the present invention better understood and enable those skilled in the art to practice the present invention, the following embodiments are further described, but the present invention is not limited to the following embodiments.
The following test methods and detection methods, unless otherwise specified, are conventional methods; the reagents and starting materials are all commercially available, unless otherwise specified.
Example 1
A method for promoting rapid germination of rhus chinensis seeds comprises the following steps:
s1, seed screening
Selecting rhus chinensis seeds with the purity of 92.1 percent, the thousand seed weight of 10.09g, the hard rate of 90 percent, the water content of 0.031 percent and the seed viability of 90 percent as qualified seeds.
S2, Disinfection of seeds
Soaking 1g of qualified seeds in S1 in 100ml of 1% potassium permanganate solution for 3min, taking out, and cleaning to obtain sterilized seeds;
s3, soaking the seeds in water
Soaking the sterilized seeds of S2 in 10ml of 80 deg.C hot water for 20min to obtain hot-dipped seeds; soaking the hot-dipped seeds in 10ml of room-temperature water for 20min again to obtain water-soaked seeds;
s4, soaking the seeds with gibberellin
Soaking the water soaked seeds obtained in the step S3 in 10ml of gibberellin solution with the concentration of 50mg/L for 24h to obtain medicine soaked seeds;
s5, accelerating germination of seeds
And (4) placing the soaked seeds obtained in the step (S4) in an environment with the temperature of 20 ℃ and the humidity of 60% for accelerating germination to obtain the germinated rhus chinensis seeds.
Example 2
A method for promoting rapid germination of rhus chinensis seeds comprises the following steps:
selecting rhus chinensis seeds with the cleanliness of 100%, the thousand seed weight of 10.79g, the hard seed rate of 94%, the water content of 0.040% and the seed viability of 100% as quality-qualified seeds.
S2, Disinfection of seeds
Soaking 5g of qualified seeds in S1 in 100ml of 5% potassium permanganate solution for 10min, taking out, and cleaning to obtain sterilized seeds;
s3, soaking the seeds in water
Soaking 1.1g of S2 sterilized seeds in 10ml of 80 deg.C hot water for 30min to obtain hot-dipped seeds; soaking the hot-dipped seeds in 10ml of room-temperature water for 30min again to obtain water-soaked seeds;
s4, soaking the seeds with gibberellin
Soaking the water soaked seeds obtained in the step S3 in 10ml of gibberellin solution with the concentration of 55mg/L for 24h to obtain medicine soaked seeds;
s5, accelerating germination of seeds
And (4) placing the soaked seeds obtained in the step (S4) in an environment with the temperature of 30 ℃ and the humidity of 70% for accelerating germination to obtain the germinated rhus chinensis seeds.
Example 3
A method for promoting rapid germination of rhus chinensis seeds comprises the following steps:
selecting Rhus chinensis seeds with purity of 95%, thousand seed weight of 10.55g, hard rate of 92%, water content of 0.035%, and seed viability of 95% as quality qualified seeds.
S2, Disinfection of seeds
Soaking 3g of qualified seeds in S1 in 100ml of 3% potassium permanganate solution for 6min, taking out, and cleaning to obtain sterilized seeds;
s3, soaking the seeds in water
Soaking 1g of S2 sterilized seeds in 10ml of 80 deg.C hot water for 25min to obtain hot-dipped seeds; soaking the hot-dipped seeds in 10ml of room-temperature water for 25min again to obtain water-soaked seeds;
s4, soaking the seeds with gibberellin
Soaking the water soaked seeds obtained in the step S3 in 10ml of gibberellin solution with the concentration of 53mg/L for 24 hours to obtain medicine soaked seeds;
s5, accelerating germination of seeds
And (4) placing the soaked seeds obtained in the step (S4) in an environment with the temperature of 25 ℃ and the humidity of 65% for accelerating germination to obtain the germinated rhus chinensis seeds.
In order to further illustrate the effect of the invention, the invention is also provided with a comparative example which is concretely as follows:
comparative example 1
A method for promoting rapid germination of rhus chinensis seeds comprises the following steps:
selecting Rhus chinensis seeds with purity of 95%, thousand seed weight of 10.55g, hard rate of 92%, water content of 0.035%, and seed viability of 95% as quality qualified seeds.
S2, Disinfection of seeds
Soaking 3g of qualified seeds in S1 in 100ml of 3% potassium permanganate solution for 6min, taking out, and cleaning to obtain sterilized seeds;
s3, soaking the seeds in water
Soaking 1g of S2 sterilized seeds in 10ml of 80 deg.C hot water for 25min to obtain hot-dipped seeds; soaking the hot-dipped seeds in 10ml of room-temperature water for 25min again to obtain water-soaked seeds;
s4, soaking seeds without gibberellin
S5, accelerating germination of seeds
And (4) placing the soaked seeds obtained in the step (S3) in an environment with the temperature of 25 ℃ and the humidity of 65% for accelerating germination.
Comparative example 2
A method for promoting rapid germination of rhus chinensis seeds comprises the following steps:
selecting Rhus chinensis seeds with purity of 95%, thousand seed weight of 10.55g, hard rate of 92%, water content of 0.035%, and seed viability of 95% as quality qualified seeds.
S2, Disinfection of seeds
Soaking 3g of qualified seeds in S1 in 100ml of 3% potassium permanganate solution for 6min, taking out, and cleaning to obtain sterilized seeds;
s3, soaking the seeds in water
Soaking 1g of the sterilized seeds of S2 in 10ml of room-temperature water for 25min to obtain water-soaked seeds;
s4, soaking the seeds with gibberellin
Soaking the water soaked seeds obtained in the step S3 in 10ml of gibberellin solution with the concentration of 53mg/L for 24 hours to obtain medicine soaked seeds;
s5, accelerating germination of seeds
And (4) placing the soaked seeds obtained in the step (S4) in an environment with the temperature of 25 ℃ and the humidity of 65% for accelerating germination.
The germination vigor, the number of days of germination, the germination rate, the number of days of germination, and the germination index of the rhus chinensis seeds in each of examples and comparative examples were measured according to the treatment methods for promoting rapid germination of rhus chinensis seeds set in examples 1 to 3 and comparative examples 1 to 2, and the results are shown in table 1 below:
TABLE 1 Rhus chinensis seed Germination
As can be seen from Table 1: compared with the comparative example 1 (without gibberellin soaking) and the comparative example 2 (without hot water soaking), the method for promoting rapid germination of rhus chinensis seeds provided by the embodiments 1 to 3 of the present invention can enable the germination vigor of rhus chinensis seeds to reach 27%, the germination vigor days to be 7 days, the germination rate to be 63.5%, the germination days to be 8 days, and the germination index to be 10.12.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (8)
1. A method for promoting rhus chinensis seeds to germinate rapidly is characterized by comprising the following steps:
s1, seed screening
Selecting qualified rhus chinensis seeds;
s2, Disinfection of seeds
Soaking the qualified seeds in the S1 in a potassium permanganate solution, taking out and cleaning to obtain sterilized seeds;
s3, soaking the seeds in water
Soaking the sterilized seeds of S2 in hot water to obtain hot-dipped seeds; soaking the hot-dipped seeds in water at room temperature again to obtain water-soaked seeds;
s4, soaking the seeds with gibberellin
Soaking the water soaked seeds obtained in the step S3 in gibberellin solution to obtain medicine soaked seeds;
s5, accelerating germination of seeds
And (4) placing the soaked seeds obtained in the step (S4) in an environment with the temperature of 20-30 ℃ and the humidity of 60-70% for accelerating germination.
2. The method for promoting rapid germination of rhus chinensis seeds as claimed in claim 1, wherein in S1, the cleanliness of the qualified rhus chinensis seeds is 92.1-100%, the thousand seed weight is 10.09-10.79 g, the hard rate is 90-94%, the water content is 0.031-0.040%, and the seed viability is 90-100%.
3. The method for promoting rhus chinensis seeds to germinate rapidly according to claim 1, wherein in S2, the mass concentration of the potassium permanganate solution is 1% -5%, and the soaking time is 3-10 min.
4. The method for promoting rapid germination of rhus chinensis seeds as claimed in claim 3, wherein in S2, the material-liquid ratio of the qualified seeds to the potassium permanganate solution is 1-5 g:100 ml.
5. The method for promoting rapid germination of rhus chinensis seeds as claimed in claim 1, wherein in S3, the temperature of the hot water is 80 ℃ and the soaking time is 20-30 min.
6. The method for promoting rapid germination of rhus chinensis seeds as claimed in claim 5, wherein in S3, the feed-liquid ratio of the sterilized seeds to hot water is 1-1.1 g:10 ml; the feed-liquid ratio of the hot-dipped seeds to the warm water is 1-1.1 g:10 ml.
7. The method for promoting rhus chinensis seeds to germinate rapidly according to claim 1, wherein in S4, the concentration of the gibberellin solution is 50-55 mg/L, and the soaking time is 24 h.
8. The method for promoting rapid germination of rhus chinensis seeds as claimed in claim 7, wherein in S4, the material-liquid ratio of the water soaked seeds to the gibberellin solution is 1-1.1 g:10 ml.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117859453A (en) * | 2024-03-12 | 2024-04-12 | 云南上智科技有限公司 | Rhus chinensis seed treatment and seedling culture method thereof |
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2021
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| CN109588081A (en) * | 2019-01-23 | 2019-04-09 | 中国林业科学研究院资源昆虫研究所 | A method of promoting Chinese sumac germination |
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| CN113330967A (en) * | 2021-05-17 | 2021-09-03 | 三峡大学 | Method for improving germination rate of rhus chinensis seeds on vegetation concrete restoration slope |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117859453A (en) * | 2024-03-12 | 2024-04-12 | 云南上智科技有限公司 | Rhus chinensis seed treatment and seedling culture method thereof |
| CN117859453B (en) * | 2024-03-12 | 2024-05-17 | 云南上智科技有限公司 | Rhus chinensis seed treatment and seedling culture method thereof |
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