CN102775524A

CN102775524A - Method for directly producing agarose from alga

Info

Publication number: CN102775524A
Application number: CN2011101329084A
Authority: CN
Inventors: 胡晓红; 薛冬梅; 高金华
Original assignee: Individual
Current assignee: Individual
Priority date: 2011-05-12
Filing date: 2011-05-12
Publication date: 2012-11-14

Abstract

The invention relates to a method for directly producing agarose from alga, comprising the following steps: using alga as an initial raw material, removing impurities, washing, conducting alkali treatment, degelatinizing, conducting DEAE adsorption, freezing and thawing, dehydrating and drying, etc. Compared with a preparation technology of agarose by using agar as the initial raw material, some unnecessary repetitive operations are reduced, the process is simplified, the energy consumption is reduced, and manpower and material resources are saved, thus the production cost is reduced.

Description

A kind of method from marine alga direct production agarose

Technical field: a kind of is the method for the colory agarose of starting raw material direct production with seaweed plant especially gelidium class

Technical background:

Agarose (Agarose) has been widely used in separating and analysis of biomacromolecule (protein, enzyme, nucleic acid and polysaccharide).The production of agarose is raw material with agar (Agar) normally, and through certain separation and purification treatment, the sulfur agar of removing wherein (Agaropectin) obtains agarose.Sulfur agar is the complicated polysaccharide that has sulfuric ester, glucuronic acid on a kind of molecule.Its existence makes the electrophoretic property of agarose reduce greatly, and the separation, the analysis that cause biomacromolecule produce detrimentally affect.Therefore, from agar, thoroughly removing electronegative sulfur agar, is crucial measure in the agarose production technique.

Produce the agar technological process from marine alga (like gelidium); Not only need gelidium impurity elimination, water logging be washed, alkaline purification, boil glue; And must the agar glue be concentrated, dry, pulverize this process power consumption, other starting material (like gac, zeyssatite, water etc.) that also consume consuming time.In order to produce agarose, can operations such as the concentrating of agar glue, drying and pulverizing be saved, in the agar glue, directly add the DEAE Mierocrystalline cellulose while hot, the electronegative material of sulfur agar class is removed in absorption, obtains purified agarose.

Summary of the invention:

The invention provides a kind of method by marine alga direct production agarose.Said method comprises following operation steps: marine alga impurity elimination, water logging are washed, alkaline purification, boiled glue, DEAE absorption, freeze thawing dehydration and drying etc.Present method is compared with the technology that with agar is starting raw material production agarose, has saved some unnecessary repetitive operations, thereby has simplified technical process, reduces energy consumption, has saved the man power and material, has shortened the production cycle, thereby has reduced production cost.

Seaweeds raw material such as gelidium embathe with tap water and to spend the night after impurity elimination, place Nylon Bag drip washing and dehydration, and be colourless until leacheate, accounts for 20% (W/W) that drops into material weight through measuring the impurity that washing removes.

75 ℃ of 2.5h of alkaline purification: 10%NaoH intermittently stir, and put and remove by filter black alkali lye in the Nylon Bag, clean to PH with water to be about 7-8.Through the product of alkaline purification, its gel-strength promotes greatly, and sulfate radical content and ash content greatly reduce.

Boil glue: the ratio 1 of gelidium and water: 30-40 adds 1.5% acetate (V/W).Heated and boiled added zeyssatite and gac in 60 minutes then, continued to stir to boil to be about 30 minutes, immediately filtered while hot.

DEAE handles: in above-mentioned hot melt adhesive by 1: 4 ratio (W/W) drop into the DEAE Mierocrystalline cellulose, 85 ℃ intermittence stir about 3h.Vacuum filtration is put glue freeze overnight in enamel tray, and thawing next day dehydrates.

Embodiment

Embodiment 1

Gelidium changes in the 10%NaoH solution after impurity elimination, water logging bubble are cleaned, and stirred 6 hours 50 ℃ of intermittences, places Nylon Bag, and tap water is washed till PH7-8; Glue: the ratio 1 of gelidium and pure water: 30-35 (W/W), 120 ° of steaming and decocting under high pressure 1.5 hours, take out add zeyssatite (about 15%, W/W) and Powdered Activated Carbon (about 2.0%, W/W), stir about half a hour, filtered while hot; The DEAE adsorption treatment: behind acid-alkali treatment, its PH7.0-7.2, the weight ratio of add-on and glue is 4: 1,85 ℃ of stirrings are about three hours, filter the glue that obtains, and place enamel tray.Freezing (12 ℃) spend the night.Take out the dehydration of thawing next day, after the fusion more in case of necessity-freezing-dehydration impurity elimination of thawing, oven dry (60 ℃) is pulverized, and the product quality analysis result is a table 1

Table 1, product quality analysis result

Embodiment 2

Gelidium changes in the 10%NaoH solution and to stir 2.5 hours 75 ℃ of intermittences after the drip washing of impurity elimination, water logging bubble, change in the Nylon Bag, with tap water drip washing to PH7-8; Glue: the ratio of gelidium and pure water is 1: 30-35, add 1.5% (V/W) acetate simultaneously, and stirring is boiled and is about one hour; Then, add about 15% (W/W) of zeyssatite, Powdered Activated Carbon (about 2.0%, W/W); The continuation stirring heating is half hour approximately, filtered while hot; The DEAE adsorption treatment: the DEAE Mierocrystalline cellulose is behind acid-alkali treatment, and its PH is 7.0-7.2, and the DEAE fiber is 4: 1 with the ratio of melting glue; 85 ℃ of stirrings are about filtration in three hours; Glue is put in the enamel tray, and freeze overnight is taken out the dehydration of thawing next day; The fusion more in case of necessity-freezing-dehydration impurity elimination of thawing is dried at last, is pulverized.The product quality analysis result is a table 2

Table 2, product quality analysis result

Claims

1. method from marine alga direct production agarose, it is characterized in that: said method comprises with the marine alga being starting raw material, through impurity elimination, washing, alkaline purification, boil glue, freeze thawing, dehydration and drying and other steps.

2. according to claim 1, the initial seaweeds that present method adopts not only comprises the gelidium algae, and suitable river hedge belongs to marine algas such as class and tenuis Pterocladia Trentepohlia.

3. according to right 1 requirement, agar working method of the present invention is characterized in that: the gelidium after the impurity elimination, with tap water embathe, drip washing, drag for dried.

4. according to claim 1,3 said, the present invention is characterised in that: clean through water and drag for dried gelidium with 6% or 10% NaoH solution-treated.

5. according to claim 1,3,4 said, the present invention is characterised in that: NaoH solution-treated temperature has 30 ℃, 50 ℃ and 75 ℃ respectively.

6. according to claim 1,4,5 said, the present invention is characterised in that: comprehensive alkaline purification concentration, temperature and time have 6%NaoH, 50 ℃ of 6h; 75 ℃ of 2.5h of 6%NaoH; 50 ℃ of 6h of 10%NaoH; 75 ℃ of four kinds of conditions such as 2.5h of 10%NaoH are top condition with 75 ℃ of 2.5h of 10%NaoH wherein.

7. according to claim 1,6 said, the invention is characterized in: the condition of boiling glue: the gelidium that alkaline purification is good and the ratio of water are about 1: 40; The method of boiling glue has: normal pressure boils glue 2h; 120 ℃ of high pressure 2h; Add 0.75% or 1.5% acetate normal pressure 1.5h; Add 0.5% acetate and 1.0%H ₂SO ₄Normal pressure 1.5h etc.

8. according to claim 1,6,7 said; The invention is characterized in: that uses on a selective basis boils the adhesive tape part: the ratio of gelidium and water is 1: 40 (W/V); Add 0.75% or 1.5% acetate; Stirring is boiled and is added about 10% zeyssatite and about 2% activated carbon powder after one hour, fully stirs, and continues to heat half hour approximately; Filtered while hot (G2 sand core funnel) goes out glue.

9. according to claim 1,8 said, the invention is characterized in: in order further to prepare purified agarose, add the DEAE Mierocrystalline cellulose that an amount of process is given processing while hot, 85 ℃ were fully stirred 2-3 hour, and filtered while hot obtains transparent glue.DEAE Mierocrystalline cellulose and glue usage ratio (W/W) were respectively 2: 1; 4: 1; With 6: 1, wherein be advisable with 4: 1.

10. according to claim 1,9 said, the invention is characterized in: through the DEAE of peracid, alkaline purification Mierocrystalline cellulose, need transfer to PH6.2-8.5, best PH is 7.0-7.2.

11., the invention is characterized in: obtain glue through the DEAE adsorption treatment, pour in enamel tray or the Stainless Steel Disc according to claim 1,9,10 said; Freezing (-12 ℃ approximately) filter; Take out the dehydration of thawing next day, fusion once more-thaw-dewater, drying, pulverizing the back is pure white powder.