CN102217699B - Preparation method of squid skin protein active peptide - Google Patents
Preparation method of squid skin protein active peptide Download PDFInfo
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- CN102217699B CN102217699B CN2010101542729A CN201010154272A CN102217699B CN 102217699 B CN102217699 B CN 102217699B CN 2010101542729 A CN2010101542729 A CN 2010101542729A CN 201010154272 A CN201010154272 A CN 201010154272A CN 102217699 B CN102217699 B CN 102217699B
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- skin
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- squid skin
- fish
- squid
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- 241000238366 Cephalopoda Species 0.000 title claims abstract description 38
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 29
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 29
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 22
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 21
- 238000000034 method Methods 0.000 claims abstract description 13
- 230000007935 neutral effect Effects 0.000 claims abstract description 11
- 238000005406 washing Methods 0.000 claims abstract description 8
- 238000004042 decolorization Methods 0.000 claims abstract description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 18
- 239000007788 liquid Substances 0.000 claims description 15
- 239000003795 chemical substances by application Substances 0.000 claims description 10
- 239000000284 extract Substances 0.000 claims description 4
- 238000000751 protein extraction Methods 0.000 claims description 4
- 238000007654 immersion Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 3
- 238000001914 filtration Methods 0.000 abstract description 3
- 238000002791 soaking Methods 0.000 abstract description 3
- 239000003513 alkali Substances 0.000 abstract description 2
- 230000036039 immunity Effects 0.000 abstract description 2
- 210000004185 liver Anatomy 0.000 abstract description 2
- 230000004224 protection Effects 0.000 abstract description 2
- 239000000243 solution Substances 0.000 abstract 3
- 238000009931 pascalization Methods 0.000 abstract 2
- 238000004140 cleaning Methods 0.000 abstract 1
- 230000003647 oxidation Effects 0.000 abstract 1
- 238000007254 oxidation reaction Methods 0.000 abstract 1
- 230000002265 prevention Effects 0.000 abstract 1
- 239000012460 protein solution Substances 0.000 abstract 1
- JBJWASZNUJCEKT-UHFFFAOYSA-M sodium;hydroxide;hydrate Chemical compound O.[OH-].[Na+] JBJWASZNUJCEKT-UHFFFAOYSA-M 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 6
- 238000010438 heat treatment Methods 0.000 description 4
- 238000006386 neutralization reaction Methods 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 102000008186 Collagen Human genes 0.000 description 2
- 108010035532 Collagen Proteins 0.000 description 2
- 229920001436 collagen Polymers 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 206010013786 Dry skin Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000237852 Mollusca Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 230000006128 skin development Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- Peptides Or Proteins (AREA)
Abstract
The invention discloses a preparation method of a squid skin protein active peptide, which comprises the following steps: cleaning squid skin, cutting into blocks, adding NaOH water solution, soaking for 2-8 days for decolorization and further washing with water till the pH is neutral; adding CaOH2 solution, soaking for 2-4 days for color fixation, further washing with the water till the pH is neutral; processing for 0.5-1.5 hours in the water with the neutral pH at the temperature of 55-75 DEG C for filtration, removing insoluble proteins and extracting for getting squid skin protein solution; and finally performing high-pressure processing on the solution for 30-120 minutes at the temperature of 110-121 DEG C for getting the squid skin protein active peptide. The squid skin is processed by the dual-alkali process, the decolorization is firstly performed and then the color fixation is performed; the high-pressure processing method is used for processing squid skin proteins for preparing the biological active peptide, the preparation process is simple, the cost is low and the applicability is strong; and the prepared active peptide has the effects of oxidation prevention, senility resistance, liver protection, enhancement of immunity and the like.
Description
Technical field
The present invention relates to squid skin albumen deep processed product, a kind of preparation method of squid skin protein active peptide is provided, belong to practical protein Preparation technical field.
Background technology
Squid belongs to mollusk siphonopods aquatic/marine animals; Angle the development of industry along with China's ocean squid; Squid becomes the main aquatic products processing raw material of China, it is reported, the year processing capacity of China squid is about 30～400,000 tons; The squid skin that wherein produces accounts for 8～13% of processing capacity, and in the squid skin dry weight Protein content up to 70～80%.Most of factories adopt buries the method processing squid skin that abandons, and has caused the waste of squid protein resource and the pollution of environment.Therefore, the protein in the exploitation squid skin is to increase squid economic value added and one of effective way that reduces environmental pollution.
In the squid skin development and use, the decolouring of fish-skin is a difficult problem.Like the patent No. 200710013140.2, be squid skin to be cleaned the back use the NaOH solution soaking, be dipped to neutrality with dilute acid soln again; Collagen is got in extracting in hot water; Enzymolysis, the enzyme that goes out make collagen active peptide again, and it is to adopt the method for alkali soak to decolour; Adopt enzyme process to prepare active peptide, but preparation cost is higher.The present invention adopts two alkaline process to handle, and decolouring back fixation has well solved this problem earlier.Handle squid skin with the decolouring of NaOH solution earlier, utilize Ca (OH) then
2Solution is residual chromoprotein in the fish-skin fixedly, extracts squid skin albumen afterwards.In addition, when utilizing protein resource to prepare biologically active peptide, mainly be to prepare through chemical method or biological enzyme protein degradation, it is safe that wherein biological enzyme produces active peptide, can under the condition of gentleness, react, and enzymolysis process is easy to control, is widely used.But general commercially available protein enzyme price is all expensive, when adopting enzyme process to prepare active peptide, has increased the development cost of protein resource greatly, makes its range of application receive many restrictions.The present invention adopts the high-pressure water heating extraction process to handle squid skin albumen, prepares biologically active peptide.Adopt this method to reduce development cost effectively, help extensive popularization.
Summary of the invention
Technical problem to be solved by this invention is the preparation method that a kind of squid skin protein active peptide is provided to prior art, and preparation technology is simple, cost is low, application is strong.
The technical scheme that the present invention is taked for the above-mentioned primary technical problem of solution is: a kind of preparation method of squid skin protein active peptide; It is characterized in that may further comprise the steps: squid skin is cleaned be cut into bulk; Adopting the NaOH solution of 0.01～0.045mol/L is decolorizer; Mass ratio by fish-skin and decolorizer is 10%～25%, immersion 2～8d (my god); Fish-skin is through washing to pH neutrality, and adopting concentration is the Ca (OH) of 0.1%～0.4% (quality)
2Solution is color-fixing agent, and fixing residual chromoprotein in the fish-skin is 10%～25% by the mass ratio of fish-skin and color-fixing agent; Soak 2～4d (day), the fish-skin that will pass through after the previous processed is washed to pH neutrality, employing pH neutral water is the protein extraction agent; Mass ratio by fish-skin and water is 10%～25%, 55～75 ℃ handle 0.5～1.5h (hour) afterwards, filter; Remove insoluble protein; Extraction obtains the fish-skin protein liquid, and this liquid is handled 30～120min in 110-121 ℃ of high-pressure water heating, has both got the squid skin protein active peptide.
As improvement, in the said decolorization, whenever change liquid once at a distance from 12～24h.
As improvement, in the said fixation process, whenever change liquid once at a distance from 12～24h.
Compared with prior art, the invention has the advantages that: the present invention adopts two alkaline process to handle squid skins, decolouring back fixation earlier, not only good decolorizing effect but also fixing residual chromoprotein in the fish-skin; Adopt high-pressure water heating to handle the squid skin protein Preparation and go out biologically active peptide, preparation technology is simple, cost is low, application is strong; Effects such as that the active peptide that makes has is anti-oxidant, anti-ageing, protection liver, enhance immunity power.
Description of drawings
Fig. 1 is preparation technology's flow chart of the present invention.
The specific embodiment
Below in conjunction with embodiment the present invention is described in further detail.
Embodiment 1
1, gets 1KG squid skin raw material: clean, drain, be cut into bulk.
2, decolouring is handled: add the NaOH solution of 4KG 0.045mol/L, soak 2d, whenever change liquid once at a distance from 12h.
3, washing neutralization: clean to pH extremely neutral repeatedly with clear water.
4, fixation treatment: adding 4KG mass concentration is 0.4% Ca (OH)
2Solution is color-fixing agent, and fixedly residual chromoprotein in the fish-skin soaks 2d, whenever changes liquid once at a distance from 12h.
5, washing neutralization: clean to pH extremely neutral repeatedly with clear water.
6, extract albumen: adopting the pH neutral water is the protein extraction agent, adds 4KG water, handles 0.5h at 75 ℃.
7, filter: remove by filter insoluble protein, obtain the fish-skin protein liquid.
8, HIGH PRESSURE TREATMENT: the protein liquid after will filtering, in 121 ℃, high-pressure water heating is handled 30min, promptly gets the squid skin protein active peptide.
Embodiment 2
1, gets 1KG squid skin raw material: clean, drain, be cut into bulk.
2, decolouring is handled: the NaOH solution that adds 10KG 0.01mol/L is decolorizer, soaks 8d, whenever changes liquid once at a distance from 24h.
3, washing neutralization: clean to pH extremely neutral repeatedly with clear water.
4, fixation treatment: adding 10KG mass concentration is 0.1% Ca (OH)
2Solution is color-fixing agent, and fixedly residual chromoprotein in the fish-skin soaks 4d, whenever changes liquid once at a distance from 24h.
5, washing neutralization: clean to pH extremely neutral repeatedly with clear water.
6, extract albumen: adopting the pH neutral water is the protein extraction agent, adds 10KG water, handles 1.5h at 55 ℃.
7, filter: remove by filter insoluble protein.
8, HIGH PRESSURE TREATMENT: the protein liquid after will filtering, in 110 ℃, HIGH PRESSURE TREATMENT 120min promptly gets the squid skin protein active peptide.
HIGH PRESSURE TREATMENT as referred to herein is because adopt the overheated hot water greater than 100 ℃, certainly will make its steam pressure greater than the atmospheric meaning of routine, and the temperature and pressure relation is confirmed according to thermodynamic principles.
Claims (3)
1. the preparation method of a squid skin protein active peptide; It is characterized in that may further comprise the steps: squid skin is cleaned be cut into bulk; Adopting the NaOH solution of 0.01～0.045mol/L is decolorizer, is 10%～25% by the mass ratio of fish-skin and decolorizer, immersion 2～8d; Fish-skin is through washing to pH neutrality, and adopting concentration is that Ca (OH) 2 solution of 0.1%～0.4% (quality) are color-fixing agent, fixedly residual chromoprotein in the fish-skin; Mass ratio by fish-skin and color-fixing agent is 10%～25%, soaks 2～4d, and the fish-skin that will pass through after the previous processed is washed to pH neutrality; Adopting the pH neutral water is the protein extraction agent, is 10%～25% by the mass ratio of fish-skin and water, after 55～75 ℃ of processing 0.5～1.5h; Filter, remove insoluble protein, extract and obtain the fish-skin protein liquid; This liquid in 110-121 ℃ of HIGH PRESSURE TREATMENT 30～120min, is promptly got the squid skin protein active peptide.
2. preparation method according to claim 1 is characterized in that in the said decolorization, whenever changes liquid once at a distance from 12～24h.
3. preparation method according to claim 1 is characterized in that in the said fixation process, whenever changes liquid once at a distance from 12～24h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101542729A CN102217699B (en) | 2010-04-19 | 2010-04-19 | Preparation method of squid skin protein active peptide |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010101542729A CN102217699B (en) | 2010-04-19 | 2010-04-19 | Preparation method of squid skin protein active peptide |
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| CN102217699A CN102217699A (en) | 2011-10-19 |
| CN102217699B true CN102217699B (en) | 2012-08-22 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103947818B (en) * | 2014-04-22 | 2016-01-20 | 钦州市山海生物科技有限公司 | A kind of preparation method of squid active peptide |
| CN105325670A (en) * | 2015-09-11 | 2016-02-17 | 舟山福氏食品科技有限公司 | Processing method for preparing biological enzymatic hydrolysis protein from squid leftovers |
| CN105925649B (en) * | 2016-05-31 | 2020-01-07 | 舟山赛莱特海洋科技有限公司 | Preparation method of low-molecular-weight degreased squid protein functional active peptide |
| CN106962940A (en) * | 2017-02-13 | 2017-07-21 | 浙江海洋大学 | A kind of preparation method of compound squid protein oral liquor |
| CN111329030A (en) * | 2020-04-09 | 2020-06-26 | 集美大学 | Method for producing seafood seasoning by using eel processing by-product |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101003827A (en) * | 2007-01-09 | 2007-07-25 | 中国海洋大学 | Method for preparing bioactive peptide of collagen from squid skin, and application |
| CN101343653A (en) * | 2008-08-22 | 2009-01-14 | 中国海洋大学 | Preparation method for holothurian collagen active peptide and application thereof |
| CN101407834A (en) * | 2008-11-05 | 2009-04-15 | 泰祥集团技术开发有限公司 | Method for preparing collagen peptide by using cod skin |
| CN101607982A (en) * | 2008-06-19 | 2009-12-23 | 陈栋梁 | A kind of fish skin collagen peptide of Pear Power effect and preparation technology thereof and purposes |
| CN101628938A (en) * | 2009-08-12 | 2010-01-20 | 江苏省海洋资源开发研究院 | Extraction technology of macromolecular collagen protein |
-
2010
- 2010-04-19 CN CN2010101542729A patent/CN102217699B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101003827A (en) * | 2007-01-09 | 2007-07-25 | 中国海洋大学 | Method for preparing bioactive peptide of collagen from squid skin, and application |
| CN101607982A (en) * | 2008-06-19 | 2009-12-23 | 陈栋梁 | A kind of fish skin collagen peptide of Pear Power effect and preparation technology thereof and purposes |
| CN101343653A (en) * | 2008-08-22 | 2009-01-14 | 中国海洋大学 | Preparation method for holothurian collagen active peptide and application thereof |
| CN101407834A (en) * | 2008-11-05 | 2009-04-15 | 泰祥集团技术开发有限公司 | Method for preparing collagen peptide by using cod skin |
| CN101628938A (en) * | 2009-08-12 | 2010-01-20 | 江苏省海洋资源开发研究院 | Extraction technology of macromolecular collagen protein |
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Inventor after: He Pengfei Inventor before: Fang Xubo Inventor before: Chen Xiaoe Inventor before: Yu Hui Inventor before: Chen Jing Inventor before: Xie Ningning |
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Effective date of registration: 20170428 Address after: 100070 Liu Zhuangzi, Beijing, Fengtai District, building 14, A115, 119 Patentee after: Beijing Tong De Hua Technology Co., Ltd. Address before: 316004 College of food and pharmacy, Zhejiang Ocean University, Zhoushan, Zhejiang, China Patentee before: Zhejiang Ocean University |
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Effective date of registration: 20201221 Address after: 224000 room 1024, business building, comprehensive free trade zone, 18 hope Avenue South Road, Yancheng Economic and Technological Development Zone, Jiangsu Province Patentee after: Jiangsu Yusheng Technology Co.,Ltd. Address before: 100070 a115, 14th floor, 119 liuzhuangzi, Fengtai District, Beijing Patentee before: Beijing Tong De Hua Technology Co.,Ltd. |
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Effective date of registration: 20210302 Address after: 224002 room 1209, business building, comprehensive bonded zone, No. 18, South hope Avenue, Yancheng Economic and Technological Development Zone, Jiangsu Province Patentee after: Jiangsu Yanzong Industry Investment Development Co.,Ltd. Address before: 224000 room 1024, business building, comprehensive free trade zone, 18 hope Avenue South Road, Yancheng Economic and Technological Development Zone, Jiangsu Province Patentee before: Jiangsu Yusheng Technology Co.,Ltd. |