GB2330516A - Cryopreservation of cell suspensions - Google Patents
Cryopreservation of cell suspensions Download PDFInfo
- Publication number
- GB2330516A GB2330516A GB9722156A GB9722156A GB2330516A GB 2330516 A GB2330516 A GB 2330516A GB 9722156 A GB9722156 A GB 9722156A GB 9722156 A GB9722156 A GB 9722156A GB 2330516 A GB2330516 A GB 2330516A
- Authority
- GB
- United Kingdom
- Prior art keywords
- temperature
- sample
- samples
- rate
- nucleation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000005138 cryopreservation Methods 0.000 title claims abstract description 13
- 239000006285 cell suspension Substances 0.000 title claims abstract description 9
- 238000000034 method Methods 0.000 claims abstract description 27
- 230000008859 change Effects 0.000 claims abstract description 11
- 230000009467 reduction Effects 0.000 claims abstract description 11
- 230000008018 melting Effects 0.000 claims abstract description 10
- 238000002844 melting Methods 0.000 claims abstract description 10
- 230000003247 decreasing effect Effects 0.000 claims abstract description 6
- 239000010902 straw Substances 0.000 claims description 21
- 238000010899 nucleation Methods 0.000 claims description 18
- 230000006911 nucleation Effects 0.000 claims description 17
- 238000001816 cooling Methods 0.000 claims description 15
- 230000006910 ice nucleation Effects 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 9
- 238000010587 phase diagram Methods 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 5
- 239000012620 biological material Substances 0.000 claims description 4
- 239000010409 thin film Substances 0.000 claims description 4
- 238000011067 equilibration Methods 0.000 claims description 3
- 239000000725 suspension Substances 0.000 claims description 3
- 230000003287 optical effect Effects 0.000 claims description 2
- 238000009529 body temperature measurement Methods 0.000 claims 1
- 230000008023 solidification Effects 0.000 abstract description 8
- 238000007711 solidification Methods 0.000 abstract description 8
- 239000013078 crystal Substances 0.000 abstract description 3
- 239000000523 sample Substances 0.000 description 28
- 230000008014 freezing Effects 0.000 description 13
- 238000007710 freezing Methods 0.000 description 13
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 12
- 239000000243 solution Substances 0.000 description 10
- 210000004027 cell Anatomy 0.000 description 6
- 239000002577 cryoprotective agent Substances 0.000 description 5
- 238000010257 thawing Methods 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 229920003023 plastic Polymers 0.000 description 4
- 239000004033 plastic Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 230000000959 cryoprotective effect Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 210000002257 embryonic structure Anatomy 0.000 description 2
- 230000005496 eutectics Effects 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 210000001161 mammalian embryo Anatomy 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 210000000287 oocyte Anatomy 0.000 description 2
- 238000002103 osmometry Methods 0.000 description 2
- 230000003204 osmotic effect Effects 0.000 description 2
- 239000012071 phase Substances 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 241000272194 Ciconiiformes Species 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- 230000005679 Peltier effect Effects 0.000 description 1
- 229920005439 Perspex® Polymers 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000000113 differential scanning calorimetry Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 239000011810 insulating material Substances 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 239000013307 optical fiber Substances 0.000 description 1
- 239000007793 ph indicator Substances 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 239000013526 supercooled liquid Substances 0.000 description 1
- 230000002381 testicular Effects 0.000 description 1
- 239000002470 thermal conductor Substances 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 238000011179 visual inspection Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0278—Physical preservation processes
- A01N1/0284—Temperature processes, i.e. using a designated change in temperature over time
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
- A01N1/0252—Temperature controlling refrigerating apparatus, i.e. devices used to actively control the temperature of a designated internal volume, e.g. refrigerators, freeze-drying apparatus or liquid nitrogen baths
- A01N1/0257—Stationary or portable vessels generating cryogenic temperatures
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S128/00—Surgery
- Y10S128/27—Cryogenic
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Catching Or Destruction (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Devices That Are Associated With Refrigeration Equipment (AREA)
Priority Applications (10)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB9722156A GB2330516A (en) | 1997-10-22 | 1997-10-22 | Cryopreservation of cell suspensions |
| CA002308250A CA2308250A1 (en) | 1997-10-22 | 1998-10-22 | Method and apparatus for cryopreservation |
| EP98949129A EP1028623B1 (de) | 1997-10-22 | 1998-10-22 | Verfahren zur gefrierkonservierung |
| AU95508/98A AU750589B2 (en) | 1997-10-22 | 1998-10-22 | Method and apparatus for cryopreservation |
| GB0008526A GB2346064B (en) | 1997-10-22 | 1998-10-22 | Method and apparatus for cryopreservation |
| AT98949129T ATE250337T1 (de) | 1997-10-22 | 1998-10-22 | Verfahren zur gefrierkonservierung |
| US09/529,667 US6361934B1 (en) | 1997-10-22 | 1998-10-22 | Method and apparatus for cryopreservation |
| PCT/GB1998/003160 WO1999020104A1 (en) | 1997-10-22 | 1998-10-22 | Method and apparatus for cryopreservation |
| DE69818502T DE69818502T2 (de) | 1997-10-22 | 1998-10-22 | Verfahren zur gefrierkonservierung |
| ES98949129T ES2205554T3 (es) | 1997-10-22 | 1998-10-22 | Metodo para crioconservacion. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB9722156A GB2330516A (en) | 1997-10-22 | 1997-10-22 | Cryopreservation of cell suspensions |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| GB9722156D0 GB9722156D0 (en) | 1997-12-17 |
| GB2330516A true GB2330516A (en) | 1999-04-28 |
Family
ID=10820819
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB9722156A Withdrawn GB2330516A (en) | 1997-10-22 | 1997-10-22 | Cryopreservation of cell suspensions |
| GB0008526A Expired - Fee Related GB2346064B (en) | 1997-10-22 | 1998-10-22 | Method and apparatus for cryopreservation |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB0008526A Expired - Fee Related GB2346064B (en) | 1997-10-22 | 1998-10-22 | Method and apparatus for cryopreservation |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US6361934B1 (de) |
| EP (1) | EP1028623B1 (de) |
| AT (1) | ATE250337T1 (de) |
| AU (1) | AU750589B2 (de) |
| CA (1) | CA2308250A1 (de) |
| DE (1) | DE69818502T2 (de) |
| ES (1) | ES2205554T3 (de) |
| GB (2) | GB2330516A (de) |
| WO (1) | WO1999020104A1 (de) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005118785A1 (en) * | 2004-06-02 | 2005-12-15 | Es Cell International Pte Ltd | Cell preservation method |
| AU2005250053B2 (en) * | 2004-06-02 | 2009-08-06 | Es Cell International Pte Ltd | Cell preservation method |
| US8895236B2 (en) | 1998-05-26 | 2014-11-25 | Stephen A. Livesey | Composition for cryopreservation comprising nicotinamide, glycerol and red blood cells |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0013714D0 (en) * | 2000-06-07 | 2000-07-26 | Acton Elizabeth | Method and apparatus for freezing tissue |
| DE10251721B4 (de) * | 2002-11-06 | 2006-06-01 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Verfahren zur Beschickung von Probenkammern mit Suspensionsproben |
| DE10251722A1 (de) * | 2002-11-06 | 2004-05-27 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Träger für eine Probenkammer, insbesondere zur Kryokonservierung biologischer Proben |
| DE10251668A1 (de) * | 2002-11-06 | 2004-05-27 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Tieftemperaturspeicherung von Suspensionsproben in hängenden Probenkammern |
| GB0308360D0 (en) * | 2003-04-11 | 2003-05-14 | Acton Elizabeth | Improved method of freeze drying |
| DE10328869A1 (de) * | 2003-06-26 | 2005-01-20 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Probenaufnahmeeinrichtung und Verfahren zu deren Herstellung |
| US20050108048A1 (en) * | 2003-11-18 | 2005-05-19 | The Jackson Laboratory | Methods and system for managing mouse colonies |
| US20060063141A1 (en) | 2004-09-17 | 2006-03-23 | Mcgann Locksley E | Method of cryopreserving cells |
| US20080269549A1 (en) * | 2007-04-27 | 2008-10-30 | The Jackson Laboratory | Method, article, and apparatus for cryopreservation of biological samples |
| WO2009065585A2 (en) | 2007-11-20 | 2009-05-28 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Ultra-rapid freezing device and method |
| US9125397B2 (en) | 2009-04-29 | 2015-09-08 | Biolife Solutions, Inc. | Apparatuses and compositions for cryopreservation of cellular monolayers |
| BR112014001238A2 (pt) | 2011-07-19 | 2017-06-13 | Ovizio Imaging Systems N V | método para a detecção de células cancerosas e/ou classificação de células numa amostra de células de líquido; sistema para a detecção de células e/ou a classificação de células cancerosas numa amostra de células; método de atualização e/ou melhoria de uma base de dados que compreende os limiares ligados á informação holográfica; e base de dados de objetos |
| EP2594334A1 (de) | 2011-11-21 | 2013-05-22 | Drive O2 | Probenfläschchen zur digitalen holographischen Analyse einer flüssigen Zellprobe |
| EP2626686A1 (de) | 2012-02-13 | 2013-08-14 | Ovizio Imaging Systems NV/SA | Durchflusszytometer mit digitalem holografischen Mikroskop |
| EP2898310B1 (de) | 2012-09-20 | 2019-05-01 | Ovizio Imaging Systems NV/SA | Digitales holografisches mikroskop mit fluidsysteme |
| JP6869949B2 (ja) | 2015-07-22 | 2021-05-12 | ザ ユニバーシティ オブ ノース カロライナ アット チャペル ヒルＴｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｎｏｒｔｈ Ｃａｒｏｌｉｎａ Ａｔ Ｃｈａｐｅｌ Ｈｉｌｌ | 氷核形成剤を含む凍結融解バルブを備える流体装置と関連の操作及び分析方法 |
| EP3196631A1 (de) | 2016-01-19 | 2017-07-26 | Ovizio Imaging Systems NV/SA | Digitales holografisches mikroskop mit elektrofluidischem system, besagtes elektrofluidisches system und verfahren zur verwendung |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4799358A (en) * | 1987-01-19 | 1989-01-24 | Agrogen Stiftung | Apparatus for cooling and deep freezing samples of biological material enclosed in vessels |
| WO1991001635A2 (en) * | 1989-08-07 | 1991-02-21 | Cell Systems Limited | Cooling process and apparatus |
| WO1996002801A1 (en) * | 1994-07-16 | 1996-02-01 | Secretary Of State For Foreign And Commonwealth Affairs | Cooling assembly for cryopreservation |
| WO1996024018A1 (en) * | 1995-01-30 | 1996-08-08 | Organogenesis, Inc. | Method and package design for cryopreservation and storage of cultured tisse equivalents |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2929278A1 (de) * | 1979-07-19 | 1981-01-29 | Forschungsgesellschaft Fuer Bi | Verfahren zum eingefrieren von zellsuspensionen |
| DE3125345A1 (de) * | 1981-06-27 | 1983-01-20 | Franz Dr.-Ing. 8552 Höchstadt Dittrich | Verfahren zum automatischen gefrieren eines insbesondere biologischen materials |
| DE3238535A1 (de) * | 1982-10-18 | 1984-04-19 | Planer Products Ltd., Sunbury-on-Thames, Middlesex | Verfahren und apparat zum gesteuerten kuehlen eines produktes |
| US5045446A (en) * | 1988-08-26 | 1991-09-03 | Cryopharm Corporation | Lyophilization of cells |
| CA2051092C (en) * | 1990-09-12 | 2002-07-23 | Stephen A. Livesey | Method and apparatus for cryopreparation, dry stabilization and rehydration of biological suspensions |
| US5629145A (en) * | 1995-03-24 | 1997-05-13 | Organ, Inc. | Cryopreservation of cell suspensions |
| NL1004619C2 (nl) | 1996-11-26 | 1998-05-27 | Instituut Voor Dierhouderij En | Inrichting en werkwijze voor het invriezen van levende cellen, in het bijzonder sperma. |
-
1997
- 1997-10-22 GB GB9722156A patent/GB2330516A/en not_active Withdrawn
-
1998
- 1998-10-22 AT AT98949129T patent/ATE250337T1/de not_active IP Right Cessation
- 1998-10-22 US US09/529,667 patent/US6361934B1/en not_active Expired - Fee Related
- 1998-10-22 WO PCT/GB1998/003160 patent/WO1999020104A1/en active IP Right Grant
- 1998-10-22 EP EP98949129A patent/EP1028623B1/de not_active Expired - Lifetime
- 1998-10-22 GB GB0008526A patent/GB2346064B/en not_active Expired - Fee Related
- 1998-10-22 DE DE69818502T patent/DE69818502T2/de not_active Expired - Fee Related
- 1998-10-22 AU AU95508/98A patent/AU750589B2/en not_active Ceased
- 1998-10-22 CA CA002308250A patent/CA2308250A1/en not_active Abandoned
- 1998-10-22 ES ES98949129T patent/ES2205554T3/es not_active Expired - Lifetime
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4799358A (en) * | 1987-01-19 | 1989-01-24 | Agrogen Stiftung | Apparatus for cooling and deep freezing samples of biological material enclosed in vessels |
| WO1991001635A2 (en) * | 1989-08-07 | 1991-02-21 | Cell Systems Limited | Cooling process and apparatus |
| WO1996002801A1 (en) * | 1994-07-16 | 1996-02-01 | Secretary Of State For Foreign And Commonwealth Affairs | Cooling assembly for cryopreservation |
| WO1996024018A1 (en) * | 1995-01-30 | 1996-08-08 | Organogenesis, Inc. | Method and package design for cryopreservation and storage of cultured tisse equivalents |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8895236B2 (en) | 1998-05-26 | 2014-11-25 | Stephen A. Livesey | Composition for cryopreservation comprising nicotinamide, glycerol and red blood cells |
| WO2005118785A1 (en) * | 2004-06-02 | 2005-12-15 | Es Cell International Pte Ltd | Cell preservation method |
| GB2429717A (en) * | 2004-06-02 | 2007-03-07 | Es Cell Int Pte Ltd | Cell preservation method |
| JP2008501320A (ja) * | 2004-06-02 | 2008-01-24 | イーエス・セル・インターナショナル・プライヴェート・リミテッド | 細胞保存方法 |
| GB2429717B (en) * | 2004-06-02 | 2009-04-08 | Es Cell Int Pte Ltd | Cell preservation method |
| AU2005250053B2 (en) * | 2004-06-02 | 2009-08-06 | Es Cell International Pte Ltd | Cell preservation method |
| US9714412B2 (en) | 2004-06-02 | 2017-07-25 | Es Cell International Pte Ltd. | Cell preservation method for pluripotent stem cells |
| US10472606B2 (en) | 2004-06-02 | 2019-11-12 | Es Cell International Pte Ltd | Cell preservation method for pluripotent stem cells |
Also Published As
| Publication number | Publication date |
|---|---|
| AU750589B2 (en) | 2002-07-25 |
| WO1999020104A1 (en) | 1999-04-29 |
| GB9722156D0 (en) | 1997-12-17 |
| ES2205554T3 (es) | 2004-05-01 |
| GB2346064B (en) | 2001-07-25 |
| GB2346064A (en) | 2000-08-02 |
| ATE250337T1 (de) | 2003-10-15 |
| CA2308250A1 (en) | 1999-04-29 |
| GB0008526D0 (en) | 2000-05-24 |
| EP1028623B1 (de) | 2003-09-24 |
| EP1028623A1 (de) | 2000-08-23 |
| US6361934B1 (en) | 2002-03-26 |
| DE69818502T2 (de) | 2004-06-24 |
| AU9550898A (en) | 1999-05-10 |
| DE69818502D1 (de) | 2003-10-30 |
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