GB2205168A - In-situ measurement of the strength of adhesion to surfaces - Google Patents

In-situ measurement of the strength of adhesion to surfaces Download PDF

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Publication number
GB2205168A
GB2205168A GB08712616A GB8712616A GB2205168A GB 2205168 A GB2205168 A GB 2205168A GB 08712616 A GB08712616 A GB 08712616A GB 8712616 A GB8712616 A GB 8712616A GB 2205168 A GB2205168 A GB 2205168A
Authority
GB
United Kingdom
Prior art keywords
plate
adhesion
strength
shear stress
radius
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB08712616A
Other versions
GB8712616D0 (en
Inventor
T M Clayton
Dr S C Musgrave
Prof R E Spier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Surrey
Original Assignee
University of Surrey
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Surrey filed Critical University of Surrey
Priority to GB08712616A priority Critical patent/GB2205168A/en
Publication of GB8712616D0 publication Critical patent/GB8712616D0/en
Publication of GB2205168A publication Critical patent/GB2205168A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N19/00—Investigating materials by mechanical methods
    • G01N19/04—Measuring adhesive force between materials, e.g. of sealing tape, of coating
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N13/00—Investigating surface or boundary effects, e.g. wetting power; Investigating diffusion effects; Analysing materials by determining surface, boundary, or diffusion effects
    • G01N13/02—Investigating surface tension of liquids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2203/00—Investigating strength properties of solid materials by application of mechanical stress
    • G01N2203/0058—Kind of property studied
    • G01N2203/0089—Biorheological properties
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material

Abstract

The strength of adhesion of animal cells, microbial cells, biofilms, and other materials to surfaces, is measured by with a device which comprises a circular plate 2 with a central orifice 1 and a supply pipe 4. Feet 3 at the periphery of plate 2 space it a predetermined distance from a surface on which it stands. In use, liquid is pumped through pipe 4 and opening 1 and flows between plate 2 and the surface to remove material adhered to the surface. The radius of the zone of clearance produced by the flow is measured and used to determine the surface shear stress at the critical radius from the formula <IMAGE> where ts = surface shear stress q = flow rate u = viscosity r = critical radius h = distance between plate and surface Laminar and non-laminar flows may be used. The flow rate is constant for a fixed measuring time. <IMAGE>

Description

A Device for the In Situ Measurement of the Strength of Adhesion to Surfaces.
This invention relates to the in-situ measurement of the strengths of adhesion of animal cells, microbial cells, biofilms and other materials attached to surfaces.
The adhssion of orczaflisms an1 films to surfaces is of great importance in bioreactor design, fouling studies and various medical fields. Quantitative data about the forces necessary to remove organisms and films from materials and hence the strength of attachment of the materials to surfaces are of great interest. All the methods available to measure detachment suffer from severe disadvantages.
The major disadvantage is that samples must be brought to the testing unit and normally grown on substrata of specific dimensions (Fowler Cell Adhesion Module).
According to the present invention there is provided a cell detachment unit comprising a circular orifice in the centre of a moveable flat plate. This plate is held at a constant distance from, and horizontal to, the surface to be tested by supports around the perimeter of the plate. Liquid is ejected from the orifice at a constant rate and for a fixed time during operation. By adjusting the orifice size and liquid flow rate it is possible to induce laminar liquid flow between the plates. The radius of the zone of clearance produced by the laminar flow can be used to calculate the critical shear stress at which material is removed from the test surface.When using a single organism or film it is possible to use the device to estimate critical shear in the absence of laminar flow by calibrating the appropriate nozzle size against one which produces laminar flow.
A specific embodiment Qf the invention will now be described by way of example, with reference to the accompanying drawing in which: Figure 1 shows in cross section the device Figure 2 shows the bottom of the device.
Referring to the drawing the device consists of an orifice 1 in the centre of a circular plate 2 which has been constructed from a single block of material. A flexible piece of tubing is attached to the pipe 4 and liquid pumped through the opening 5. The three spacing legs 3, are used to maintain the device at a constant distance from the surface being investigated.
In conditions of laminar flow the following equation is used to calculate the surface shear stress at the critical radius.
Where Ts = surface shear stress Q = Flow rate u = viscosity r = critical radius h = distance between surfaces

Claims (1)

  1. CLAIMS 1: A device for the in-situ measurement of the strength of adhesion of organisms or materials to surfaces.
    2: A device as claimed in Claim 1 which utllises a defined laminar liquid flow.
    3: A device as claimed in Claim 1 or Claim 2 which utilises a non-defined liquid flow.
    4: A device as claimed in Claims, and?or 2 and 3 which is automated.
GB08712616A 1987-05-28 1987-05-28 In-situ measurement of the strength of adhesion to surfaces Withdrawn GB2205168A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB08712616A GB2205168A (en) 1987-05-28 1987-05-28 In-situ measurement of the strength of adhesion to surfaces

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB08712616A GB2205168A (en) 1987-05-28 1987-05-28 In-situ measurement of the strength of adhesion to surfaces

Publications (2)

Publication Number Publication Date
GB8712616D0 GB8712616D0 (en) 1987-07-01
GB2205168A true GB2205168A (en) 1988-11-30

Family

ID=10618084

Family Applications (1)

Application Number Title Priority Date Filing Date
GB08712616A Withdrawn GB2205168A (en) 1987-05-28 1987-05-28 In-situ measurement of the strength of adhesion to surfaces

Country Status (1)

Country Link
GB (1) GB2205168A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102590084A (en) * 2012-03-06 2012-07-18 西安交通大学 Apparatus and method for evaluating adhesion performance of gelled vegetable oil

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109975178B (en) * 2019-04-22 2021-12-17 江西辰鑫科技有限公司 Adhesive flow testing device

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB769089A (en) * 1954-05-21 1957-02-27 Sheffield Corp Method of and apparatus for gauging the friability of different portions of a grinding wheel or the like
GB890720A (en) * 1958-05-06 1962-03-07 British Rayon Res Ass A method and means for testing the degree of raise of a raised fabric
GB1176553A (en) * 1966-03-29 1970-01-07 Baldwin Gegenheimer Corp Air Detector for Webs
GB1204237A (en) * 1967-07-27 1970-09-03 Cluett Peabody & Company Inc Apparatus for testing wearability of fabrics
GB2147704A (en) * 1983-10-04 1985-05-15 Cottbus Wohnungsbau Process for testing non-homogeneous building materials
SU1308882A2 (en) * 1986-01-08 1987-05-07 Ростовский научно-исследовательский институт технологии машиностроения Method of determining strength of coating-to-substrate adhesion

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB769089A (en) * 1954-05-21 1957-02-27 Sheffield Corp Method of and apparatus for gauging the friability of different portions of a grinding wheel or the like
GB890720A (en) * 1958-05-06 1962-03-07 British Rayon Res Ass A method and means for testing the degree of raise of a raised fabric
GB1176553A (en) * 1966-03-29 1970-01-07 Baldwin Gegenheimer Corp Air Detector for Webs
GB1204237A (en) * 1967-07-27 1970-09-03 Cluett Peabody & Company Inc Apparatus for testing wearability of fabrics
GB2147704A (en) * 1983-10-04 1985-05-15 Cottbus Wohnungsbau Process for testing non-homogeneous building materials
SU1308882A2 (en) * 1986-01-08 1987-05-07 Ростовский научно-исследовательский институт технологии машиностроения Method of determining strength of coating-to-substrate adhesion

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
WO 87/01804 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102590084A (en) * 2012-03-06 2012-07-18 西安交通大学 Apparatus and method for evaluating adhesion performance of gelled vegetable oil
CN102590084B (en) * 2012-03-06 2013-08-14 西安交通大学 Apparatus and method for evaluating adhesion performance of gelled vegetable oil

Also Published As

Publication number Publication date
GB8712616D0 (en) 1987-07-01

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WAP Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1)