CN207937254U - The detecting system of the mechanical property of biological tissue - Google Patents
The detecting system of the mechanical property of biological tissue Download PDFInfo
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- CN207937254U CN207937254U CN201721909368.5U CN201721909368U CN207937254U CN 207937254 U CN207937254 U CN 207937254U CN 201721909368 U CN201721909368 U CN 201721909368U CN 207937254 U CN207937254 U CN 207937254U
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Abstract
The utility model is related to a kind of detecting systems of the mechanical property of biological tissue.The detecting system of the mechanical property of the biological tissue includes pressure head component, detection part and displacement sensor.For squeezing biological tissue, pressure head component can move pressure head component；Detection part can be moved with pressure head component, detection part can be in pressure head component and while contact biological tissue and contact biological tissue, and detection part can also stop moving with pressure head component when with contact biological tissue, and pressure head component is enable individually to continue to move to squeeze biological tissue；Displacement sensor can detect the displacement of pressure head component movement, and displacement sensor can start to detect the real-time displacement of pressure head component in detection part and contact biological tissue.The measurement result of the detecting system of the mechanical property of above-mentioned biological tissue is more accurate.
Description
Technical field
The utility model is related to biotechnology, more particularly to a kind of detecting system of the mechanical property of biological tissue.
Background technology
It is directed to the mechanics study of biological tissue's organ at present, is usually carried out under macroscopic aspect, suffers from various factors
Influence, measurement error is very big.After being increased by intraocular pressure and to be influenced such as glaucoma, for cornea is with respect to normal cornea in Chinese, deformation energy
Power is weaker, and by cornea radian, position Different Effects, measurement result is not fully identical.And under micro-nano-scale, biology
Organization mechanics characteristic is only related to the variation of tissue element level is constituted, and directly reflects the mechanical change of tissue.
The means of test biology tissue micro nanometer mechanics characteristic mainly have micro-nano indentation at present.Micro-nano impression skill
Art is broadly divided into two kinds：One kind is atomic force microscope indentation, but since loading force range is receiving ox grade, institute's sample is hard
Degree is limited in scope, and sample is limited by surface roughness, and probe is easily damaged and cumbersome；Another kind is Nanoindentation, is led to
Normal nano impress loading force is receiving ox~milli ox grade, and the resolution ratio of power can receive ox down to 1, and displacement resolution can be down to 0.01
Nanometer；And the pressure head material of device is usually from diamond, elasticity modulus 72Gpa, it is superhard material, is hardly damaged, by surface
Roughness influences smaller；Therefore, such technology can accurately measure the micro mechanical property of material, and have larger sample
Hardness test range.However, Nanoindentation is in processing soft material, such as high molecular material or biologic soft tissue sample
When, since sample is often very soft, it cannot accurately determine initial contact point, cause the error of testing result larger.
Utility model content
Based on this, it is necessary to provide a kind of measurement result more accurately detecting system of the mechanical property of biological tissue.
A kind of detecting system of the mechanical property of biological tissue, the mechanical property for detecting biological tissue, including：
Driver；
The pressure head component of the biological tissue can be squeezed, can be moved, the pressure head component is driven with the driver
Connection, so that the driver can drive the pressure head component to move；
Detection part can be moved with the pressure head component, and can be connect in the pressure head component and the biological tissue
It can also stop when the detection part is with the contact biological tissue with the contact biological tissue, the detection part while touching
It is only moved with the pressure head component, and so that the pressure head component is individually continued to move to squeeze the biological tissue；And
Displacement sensor can detect the displacement of the pressure head component movement, and institute's displacement sensors can be described
Start to detect the real-time displacement of the pressure head component when detection part is with the contact biological tissue.
Since the detection part of the detecting system of the mechanical property of above-mentioned biological tissue can be moved with pressure head component, and detect
Part can be in pressure head component and while contact biological tissue and contact biological tissue, displacement sensor can be in detection parts and life
Object tissue starts to detect the real-time displacement of pressure head component when contacting, then, pressure head can relatively accurately be known by detection part
The time of component and contact biological tissue, and displacement sensor is enable relatively accurately to connect at the beginning of the pressure head component and biological tissue
Start the real-time displacement of detection pressure head component when tactile so that the real-time displacement that displacement sensor detects is more nearly pressure head component
The depth of practical indentation biological tissue, is conducive to the accuracy for improving detection data；Simultaneously as detection part can also with life
Stop moving with pressure head component when object tissue contacts, and so that pressure head component is individually moved to squeeze biological tissue, thus
While realizing that pressure head component squeezes biological tissue, prevent detection part continue to move with pressure head component to be expressed to biological tissue and
The accuracy of test is influenced, therefore, the measurement result of the detecting system of the mechanical property of above-mentioned biological tissue is more accurate.
The detection part can be fixed with the pressure head component in one of the embodiments, so that the detection part energy
Enough to be moved with the pressure head component, the detection part can also be detached with the pressure head component, so that the detection part can stop
Only moved with the pressure head component.
Further include that can control fixing and detaching for the detection part and the pressure head component in one of the embodiments,
Control piece, the control piece can be powered when so that the detection part is fixed with the pressure head component, make when power is off described in
Detection part is detached with the pressure head component.
The detection part is tubular in one of the embodiments, and the pressure head component includes compression bar and pressure head, the pressure
Bar can move, and the pressure head is fixedly connected with the compression bar, so that the pressure head can be moved with the compression bar, and the pressure
Head can be contained in the detection part, and the pressure head can also stretch out the detection part, and the pressure head is contained in the detection
When in part, side of the pressure head far from the compression bar is concordant with the detection part, so that the pressure head and the detection part energy
It is enough simultaneously with the contact biological tissue, and when the detection part is with the contact biological tissue, the detection part stops with institute
State compression bar movement, and enable the compression bar that the pressure head individually to be driven to continue to move to so that the pressure head can stretch out it is described
Detection part and squeeze the biological tissue.
Further include sample fixing device in one of the embodiments, the sample fixing device includes：
Incubator can contain the biological tissue, inlet and liquid outlet are offered on the incubator；
The liquid storage bottle that liquid can be contained is connected to the inlet, and the liquid in the liquid storage bottle can flow into described
In incubator, wherein the liquid in the incubator can be flowed out from the liquid outlet；
Temperature control part is electrically connected with the control device, and the temperature control part can control the temperature in the incubator.
Further include inlet tube in one of the embodiments, one end of the inlet tube is connected to the liquid storage bottle, another
End is connected to the inlet, and height of the inlet tube close to one end of the liquid storage bottle is close higher than the inlet tube described
The height of one end of inlet.
Further include the liquid inlet control valve being mounted on the inlet tube in one of the embodiments, the feed liquor control
Valve can control the flowing velocity of the liquid in the inlet tube.
Further include the liquid level gauge being mounted in the incubator in one of the embodiments, the liquid level gauge can be examined
The level value in the incubator is surveyed, the liquid inlet control valve can control the liquid in the inlet tube according to the level value
Flowing velocity.
Further include the waste liquid bottle being connected to the liquid outlet, the liquid in the incubator in one of the embodiments,
It can be flowed into the waste liquid bottle by the liquid outlet.
Further include in one of the embodiments, outlet tube that one end is connected to the liquid outlet and mounted on it is described go out liquid
Liquid control valve on pipe, the liquid control valve can control the flowing velocity of the liquid in the outlet tube.
Further include the fixation kit being mounted in the incubator, the fixation kit packet in one of the embodiments,
Fitting is included, the fitting can be abutted against with the biological tissue, so that the biological tissue can be clamped and fixed in
Between the fitting and the inner wall of the incubator.
The incubator has bottom wall in one of the embodiments, and the fitting can be towards closer or far from described
The direction of the bottom wall of incubator is slided, and the fitting can be abutted against with the biological tissue, and the fitting can be with
The bottom wall of the incubator grips the biological tissue jointly.
The fixation kit further includes the fixing piece being fixed in the incubator in one of the embodiments, described
Fitting includes the abutting part of rod-shaped mounting portion and the bar shaped extended from the bending of one end of the mounting portion, the mounting portion peace
It on the fixing piece, and can be slided towards the direction closer or far from the incubator bottom wall, the mounting portion can also
Enough to be rotated relative to the fixing piece, the abutting part can be abutted against with the biological tissue.
The abutting part is equipped with arc convex in one of the embodiments, and the arc convex can be with the life
Object tissue abuts against.
Further include gas supply assembly in one of the embodiments, the gas supply assembly is connected to the incubator, the confession
Pneumatic module can input carbon dioxide into the incubator.
Further include in one of the embodiments, observation component and can movably mobile station, the observation component can
The image of the biological tissue in the incubator is acquired, the mobile station is fixedly connected with the incubator, the movement
Platform can drive the incubator to move according to the image.
The observation component includes object lens and light source in one of the embodiments, and the object lens can acquire the training
The image of the biological tissue in case is supported, the light source can give the object lens to illuminate.
Description of the drawings
Fig. 1 is that the pressure head of the pressure head component of the detecting system of the mechanical property of the biological tissue of an embodiment has been pressed into
Structural schematic diagram when biological tissue；
Fig. 2 is that pressure head component, detection part and the control piece of the detecting system of the mechanical property of biological tissue shown in FIG. 1 exist
Structural schematic diagram when pressure head is housed in detection part；
Fig. 3 is the structural schematic diagram of the sample fixing device of the detecting system of the mechanical property of biological tissue shown in FIG. 1；
Fig. 4 is that the structure that incubator, fixation kit and the liquid level gauge of sample fixing device shown in Fig. 3 fit together is shown
It is intended to；
Fig. 5 is the incubator of sample fixing device shown in Fig. 3 and fixation kit fits together and incubator is omitted
The structural schematic diagram of another angle of roof；
Fig. 6 is the structural schematic diagram of fixation kit shown in 4；
Fig. 7 is the structural schematic diagram that incubator shown in Fig. 4 and fixation kit are fixed with detected sample；
Fig. 8 is the flow chart of the detection method of the mechanical property of the biological tissue of embodiment one.
Specific implementation mode
The utility model is more fully retouched below with reference to relevant drawings for the ease of understanding the utility model,
It states.The preferred embodiment of the utility model is given in attached drawing.But the utility model can come in many different forms
It realizes, however it is not limited to embodiment described herein.Make to the utility model on the contrary, purpose of providing these embodiments is
The understanding of disclosure is more thorough and comprehensive.
It should be noted that when element is referred to as " being fixed on " another element, it can be directly on another element
Or there may also be elements placed in the middle.When an element is considered as " connection " another element, it can be directly connected to
To another element or it may be simultaneously present centering elements.Term as used herein " vertical ", " horizontal ", " left side ",
" right side " and similar statement are for illustrative purposes only.
Unless otherwise defined, all of technologies and scientific terms used here by the article is led with the technology for belonging to the utility model
The normally understood meaning of technical staff in domain is identical.Terminology used in the description of the utility model herein only be
The purpose of description specific embodiment, it is not intended that in limitation the utility model.
As shown in Figure 1, the detecting system 10 of the mechanical property of the biological tissue of an embodiment, is a kind of impression test system
System, the mechanical property for detecting biological tissue 20.Wherein, biological tissue 20 (can usually paste for biologic soft tissue, cell
Parietal cell) etc. viscoelastic materials.The detecting system 10 of the mechanical property of the biological tissue includes holder (not shown), control device
100, driver 200, pressure head component 300, detection part 400, control piece 500, displacement sensor 600, sample fixing device 700,
Mobile station 800 and observation component 900.
Holder is the support member of the detecting system 10 of the mechanical property of entire biological tissue.
Control device 100 is used to control the work of each electric device of the detecting system 10 of the mechanical property of entire biological tissue
And record and the calculation processing of information.Specifically, control device 100 is computer.
Driver 200 is fixedly mounted on holder.Specifically, driver 200 is magnetic driver.Driver 200 and control
Device 100 processed is electrically connected, and control device 100 can control the work of driver 200.
Pressure head component 300 can move, and pressure head component 300 is for squeezing biological tissue 20, to realize biological tissue 20
Impression test.Wherein, pressure head component 300 is sequentially connected with driver 200, so that driver 200 being capable of drive ram component 300
It is mobile.
Detection part 400 can be moved with pressure head component 300, and can be contacted with biological tissue 20 in pressure head component 300
While contacted with biological tissue 20, can also stop with pressure when detection part 400 and biological tissue 20 contact in detection part 400
Head assembly 300 moves, and so that pressure head component 300 is individually continued to move to squeeze biologic component 20.Wherein, detection part 400
It is electrically connected with control device 100, when detection part 400 is contacted with biological tissue 20, detection part 400 can transmit a signal to control
Device 100 processed so that control device 100 whether can be contacted according to detection part 400 and biological tissue 20 control detection part 400 with
Pressure head component 300 moves and control detection part 400 stops moving with pressure head component 300.
Specifically, detection part 400 can be fixed with pressure head component 300, so that detection part 400 can be with pressure head component 300
Mobile, detection part 400 can also be detached with pressure head component 300, so that detection part 400 can stop moving with pressure head component 300.
Also referring to Fig. 2, more specifically, detection part 400 is tubular, pressure head component 300 includes compression bar 310 and pressure head
320, compression bar 310 can move, and pressure head 320 is fixedly connected with compression bar 310, so that pressure head 320 can be moved with compression bar 310, and
Pressure head 320 can be contained in detection part 400, and pressure head 320 can also stretch out detection part 400, and pressure head 320 is contained in detection part
When in 400, side of the pressure head 320 far from compression bar 310 is concordant with detection part 400, so that pressure head 320 and detection part 400 can be same
When contacted with biological tissue 20, and when detection part 400 and biological tissue 20 contact, detection part 400 stops moving with compression bar 310,
And compression bar 310 is enable individually to be continued to move to dynamic head 320, so that pressure head 320 can stretch out detection part 400 and squeeze biology
Tissue 20.Specifically in the illustrated embodiment, one end of compression bar 310 is sequentially connected with driver 200, so that 200 energy of driver
Enough the movement of driving compression bar 310 is moved with band dynamic head 320, and pressure head 320 is fixed with the one end of compression bar 310 far from driver 200 to be connected
It connects.When pressure head 320 is contained in detection part 400, the one end of compression bar 310 far from pressure head 320 is housed in detection part 400.Detection
When part 400 is moved with the direction of compression bar 310 towards close biological tissue 20, side and detection part of the pressure head 320 far from compression bar 310
400 is concordant.Wherein, pressure head 320 is nano-indentators.
Since biological tissue 20 usually has more even curface, by side of the pressure head 320 far from compression bar 310
Setting concordant with detection part 400, then, it is moved simultaneously towards close to the direction of biological tissue 20 in pressure head 320 and detection part 400
When, it will be able to so that pressure head 320 and detection part 400 is contacted simultaneously with biological tissue 20 as far as possible.
Specifically, detection part 400 has opening and the bottom opposite with being open, and is offered on the bottom of detection part 400
Through hole 410, compression bar 310 is arranged in through hole 410, and when pressure head 320 is contained in detection part 400, pressure head 320 is far from compression bar
310 side is concordant with the open end of detection part 400.
Specifically, detection part 400 is sensing detector.The internal diameter of detection part 400 is more than the width of biological tissue 20, so that
Detection part 400 can be contacted preferably with biological tissue 20.
Control piece 500 can control fixing and detaching for detection part 400 and pressure head component 300, and control piece 500 can be logical
So that detection part 400 is fixed with pressure head component 300 when electric, detection part 400 is made to be detached with pressure head component 300 when power is off.Wherein, it controls
Product 500 is electrically connected with control device 100, so that control device 100 can control the energization and power-off of control piece 500, that is, is controlled
Whether device 100 processed can contact the work of control control piece 500 according to detection part 400 with biological tissue 20, to control detection
The fixation and separation of part 400 and compression bar 310.The energization and power-off of control piece 500 are controlled by control device 100, so that real
The fixation and separation of existing detection part 400 and compression bar 310.
Specifically in the present embodiment, control piece 500 is electromagnet, and control piece 500 is fixedly set on compression bar 310, and
Control piece 500 is housed in detection part 400, and control piece 500 can control fixing and detaching for detection part 400 and compression bar 310.I.e.
Detection part 400 can be fixedly connected by control piece 500 when being powered with compression bar 310, so that compression bar 310 can drive detection part
400 move with compression bar 310, and control piece 500 can also be such that detection part 400 is detached with compression bar 310 when power is off, so that detection part
400 can stop moving with compression bar 310.
Specifically, one week container 420 is arranged around through hole for the inner surface concave shape cyclization of the bottom of detection part 400,
Control piece 500 can be contained in container 420, and the open end of pressure head 320 one end far from compression bar 310 and detection part 400
When concordant, control piece 500 is contained in container 420.
It should be noted that control piece 500 is not limited to be housed in detection part 400, detection part 400 and control piece 500 are also
Can be setting up and down, at this point, detection part 400 is also not necessarily limited to as tubular structure.Alternatively, control piece 500 is not limited to be fixed on pressure
On bar 310, control piece 500 can also be fixed on detection part 400.Control piece 500 is also not necessarily limited to as electromagnet, for example, control
Part 500 or with electronic lock, when detection part 400 is moved with pressure head component 300, control piece 500 is by detection part 400 and compression bar
310 lock together, and when detection part 400 and biological tissue 20 contact, are unlocked by control device 100 so that detection part 400 with
Compression bar 310 detaches.
It is appreciated that realizing fixing and detach and being also not necessarily limited to for electric control, may be used also for detection part 400 and pressure head component 300
To be manually controlled by the way that hand control valve is arranged.
It is appreciated that realize that detection part 400 can be moved with pressure head component 300, and can be in pressure head component 300 and life
Object tissue 20 contact while contacted with biological tissue 20, and detection part 400 can also be in detection part 400 and biological tissue 20
Stop being not limited to use aforesaid way with the movement of pressure head component 300 when contact, for example, it is also possible to make driver 200 while controlling
Detection part 400 and pressure head component 300, alternatively, two drivers 200 are arranged simultaneously, two drivers 200 control detection part respectively
400 and pressure head component 300.
Specifically, when detection part 400 is detached with compression bar 310 in order to prevent, detection part 400 will not voluntarily be fallen, detection part
400 can be rack-mount by support element.For example, support element can be support spring, one end of support element and holder are solid
Fixed connection, the other end are fixedly connected with detection part 400, then, when detection part 400 and compression bar 310 are without fixing, support element energy
Enough reset detection part 400.
Displacement sensor 600 can detect the displacement of the movement of pressure head component 300, and displacement sensor 600 can detect
Part 400 starts to detect the real-time displacement of pressure head component 300 when contacting with biological tissue 20.Wherein, displacement sensor 600 and control
Device 100 is electrically connected, and the real-time displacement of detection can be transferred to control device 100 by displacement sensor 600.
Specifically, displacement sensor 600 is capacitive displacement transducer, and displacement sensor 600 is set with the interval of compression bar 310
It sets, displacement sensor 600 can detect the real-time displacement of compression bar 310, since displacement sensor 600 is in detection part 400 and biology
Start the real-time displacement of detection pressure head component 300, therefore, the real-time displacement that displacement sensor 600 detects when 20 contact of tissue
As pressure head 320 is pressed into the real-time deep of biological tissue 20.More specifically, displacement sensor 600 passes through elastic supporting member for supporting optical member and branch
Frame is fixedly connected.
Also referring to Fig. 3, sample fixing device 700 can make biology for splendid attire and fixed biological tissue 20
Tissue 20 is in during detection under a more normal physiological environment, to further increase the accurate of test result
Property.Wherein, sample fixing device 700 can be arranged in the lower section of pressure head 320.Specifically in the illustrated embodiment, the sample is solid
It includes incubator 710, liquid storage bottle 720, liquid inlet control valve 730, liquid level gauge 735, waste liquid bottle 740, liquid control valve to determine device 700
750, temperature control part 760, gas supply assembly 770 and fixation kit 780.
Incubator 710 can contain sample to be tested, and inlet 712 and liquid outlet 714 are offered on incubator 710.Its
In, liquid can be added from inlet 712 in incubator 710；Liquid in incubator 710 can be flowed out from liquid outlet 714.It should
Liquid is cell culture fluid or phosphate buffered saline solution, if biological tissue 20 is cell, which is usually cell culture fluid,
If biological tissue 20 is biologic soft tissue, liquids in general is phosphate buffered saline solution.
Also referring to Fig. 4, specifically, incubator 710 includes bottom wall 715, side wall 716 and roof 717,716 ring of side wall
Continuously it is arranged one week around bottom wall 715, to form the accommodating space that can house biological tissue 20, roof 717 is covered on side wall 716
On side far from bottom wall 715, and accommodating space is covered, the instrument connection worn for pressure head 320 is offered on roof 717 and (is schemed not
Show).Wherein, inlet 712 and liquid outlet 714 are opened on the side wall 716 of incubator 710.Inlet 712 arrives bottom wall 715
Height higher than liquid outlet 714 arrive bottom wall 715 height.Specifically in the illustrated embodiment, the substantially open tubular column of incubator 710
Shape structure.
Liquid storage bottle 720 can contain liquid, and liquid storage bottle 720 is connected to inlet 712, and the liquid in liquid storage bottle 720 can
It flows into incubator 710.Specifically, sample fixing device 700 further includes inlet tube 725, one end of inlet tube 725 and liquid storage bottle
720 connections, the other end is connected to inlet 712, to realize the connection of liquid storage bottle 720 and incubator 710.
Further, height of the inlet tube 725 close to one end of liquid storage bottle 720 is higher than inlet tube 725 close to inlet 712
One end height so that liquid in liquid storage bottle 720 can be flowed into voluntarily by inlet tube 725 in incubator 710, without
Driving device is set.
It should be noted that height of the inlet tube 725 close to one end of liquid storage bottle 720 is not limited to lean on higher than inlet tube 725
The height of one end of nearly inlet 712, can also be arranged to identical height, either, inlet tube 725 is close to liquid storage bottle 720
One end height less than inlet tube 725 close to the height of one end of inlet 712, at this point, setting driving device, such as water
Pump can also realize that the liquid in liquid storage bottle 720 can be flowed by inlet tube 725 in incubator 710.
Liquid inlet control valve 730 is mounted on inlet tube 725, and liquid inlet control valve 730 can control the liquid in inlet tube 725
Flow into the speed in incubator 710.Specifically, liquid inlet control valve 730 is solenoid valve.More specifically, liquid inlet control valve 730 and control
Device 100 processed is electrically connected, and control device 100 can control the work of liquid inlet control valve 730.
Liquid level gauge 735 is mounted in incubator 710, and liquid level gauge 735 can detect the liquid level in incubator 710, into hydraulic control
Valve 730 processed can flow into the flowing velocity in incubator 710 according to the liquid in Liquid level inlet tube 725.For example, when culture
When liquid level in case 710 does not reach required liquid level, liquid inlet control valve 730 controls the liquid in inlet tube 725 with maximum flow rate
It is flowed into incubator 710, so that incubator 710 can reach required liquid level in the short time, the liquid level in incubator 710
When reaching required liquid level, it is zero that liquid inlet control valve 730, which controls the flow velocity reduction of the liquid in inlet tube 725 or coutroi velocity,.Tool
Body, liquid level gauge 735 and liquid inlet control valve 730 are electrically connected with control device 100, and liquid level can be transferred to by liquid level gauge 735
Control device 100, control device 100 can be according to the work of the Liquid level liquid inlet control valve 730.
Waste liquid bottle 740 is connected to liquid outlet 714, and the liquid in incubator 710 can flow into waste liquid bottle by liquid outlet 714
In 740.Specifically, sample fixing device 700 further includes outlet tube 745, and one end of outlet tube 745 is connected to liquid outlet 714, separately
One end is connected to waste liquid bottle 740, to realize the connection of waste liquid bottle 740 and incubator 710.
Liquid control valve 750 is mounted on outlet tube 745, and liquid control valve 750 can control the liquid in outlet tube 745
The rate of outflow.Specifically, liquid control valve 750 is solenoid valve, and liquid control valve 750 can be according to Liquid level outlet tube
The flowing velocity of liquid in 745.For example, when the liquid level in incubator 710 does not reach required liquid level, liquid control valve
The flow velocity of liquid in 750 control outlet tubes 745 is zero, so that incubator 710 can reach required liquid level in the short time, when
When liquid level in incubator 710 reaches required liquid level, liquid control valve 750 controls the liquid flowing in inlet tube 725, in order to
Liquid in incubator 710 being capable of constant flow.Specifically, liquid control valve 750 is electrically connected with control device 100, control dress
The work of liquid control valve 750 can be controlled by setting 100.
Temperature control part 760 can control the temperature in incubator 710 so that in incubator 710 temperature maintain 37 DEG C~
37.5℃.Specifically, temperature control part 760 is arranged in the outside of incubator 710, and the bottom wall 715 of temperature control part 760 and incubator 710
It is fixedly connected.Temperature control part 760 is electrically connected with control device 100, and control device 100 can control the work of temperature control part 760.
Gas supply assembly 770 is connected to incubator 710, and gas supply assembly 770 can give input carbon dioxide in incubator 710
The air that volumn concentration is 5% is maintained with supplementing carbon dioxide to the biological tissue 20 in incubator 710 in incubator
The pH value of culture solution is in normal range (NR), so that biological tissue 20 (such as cell) can be in a normal life for a long time
It manages under environment, increases the time-to-live for improving biological tissue 20, be conducive to the test of long period and improve the standard of test result
True property.Specifically, gas supply assembly 770 is electrically connected with control device 100, and control device 100 controls the gas supply work of gas supply assembly.
Biological tissue 20 (such as biologic soft tissue) can be fixed in incubator 710 by fixation kit 780, to prevent to give birth to
Object tissue 20 moves in incubator 710 so that above-mentioned sample fixing device 700 also can be used in the sample of non-adherent cell
Nano-indenter test.According to Saint Venant's principle, the pressing for only treating test sample locally generates stress and Deformation Field, is pressed separate
The result that intermediate pressure section carries out nanometer mechanics measurement will be unaffected, therefore, by the way that fixation kit 780 and biological tissue 20 is arranged
Part abuts against, and can not only realize the fixation of biological tissue 20, and on test result also without influence.
It should be noted that liquid inlet control valve 730, liquid level gauge 735, liquid control valve 750, temperature control part 760 and gas supply group
Part 770 is not limited to be electrically connected in control device 100, can also control, however is uniformly controlled by control device 100, energy respectively
The intelligence of detection is enough better achieved.
Also referring to Fig. 5 and Fig. 6, specifically, fixation kit 780 is multiple, and multiple fixation kits 780 are arranged at intervals on
On the bottom wall 715 of incubator 710, and multiple fixation kits 780 are concyclic, the bottom wall of multiple fixation kits 780 and incubator 710
715 being capable of common biological tissue clamped 20.Wherein, each fixation kit 780 includes fixing piece 782 and fitting 784.
Fixing piece 782 is fixed in incubator 710.Specifically, fixing piece 782 is fixed on the bottom wall 715 of incubator 710
On.More specifically, 782 generally cylindrical structure of fixing piece, one end of fixing piece 782 is fixed with the bottom wall 715 of incubator 710 to be connected
It connects, the other end is extended vertically with respect to the bottom wall 715 of incubator 710.
It should be noted that fixing piece 782 is not limited to be fixed on the bottom wall 715 of incubator 710, it is also fixed on incubator
On 710 side wall 716.
Fitting 784 can be abutted against with biological tissue 20, so that biological tissue 20 can be clamped and fixed in fitting
Between 784 and the inner wall of incubator 710.Specifically, fitting 784 is movably mounted on fixing piece 782.Wherein, fitting
784 can slide towards the direction of the bottom wall 715 closer or far from incubator 710, and fitting 784 can be with 20 phase of biological tissue
It abuts, and fitting 784 can grip biological tissue 20 jointly with the bottom wall 715 of incubator 710.
More specifically, what fitting 784 extended including rod-shaped mounting portion 784a and from the bending of one end of mounting portion 784a
The abutting part 784b of bar shaped, mounting portion 784a are mounted on fixing piece 782, and can be towards closer or far from incubator 710
The direction of bottom wall 715 is slided, mounting portion 784a can also opposed fixed member 782 rotate, and abutting part 784b can be with biological group
20 are knitted to abut against.That is abutting part 784b can jointly coordinate with the bottom wall 715 of incubator 710 grips biological tissue 20.Tool
Body, mounting portion 784a settings coaxial with fixing piece 782.Mounting portion 784a can also 360 ° of rotations of the generation of opposed fixed member 782.
Further, abutting part 784b is equipped with arc convex 784c, and arc convex 784c can be with 20 phase of biological tissue
Abut, i.e. arc convex 784c can with the 715 common biological tissue clamped 20 of bottom wall of incubator 710, to reduce fitting 784
With the contact area of biological tissue 20, the accuracy of test result is improved.Specifically in the illustrated embodiment, arc convex 784c
Positioned at the one end of abutting part 784b far from mounting portion 784a.
Specifically, the mounting portion 784a of fitting 784 and 782 screw-thread fit of fixing piece, to by by fitting 784
Mounting portion 784a and fixing piece 782, which screw togather, can simply implement between abutting part 784b and the bottom wall 715 of incubator 710
Distance adjusting, and realize that mounting portion 784a opposed fixed members 782 rotate.
It should be noted that fixation kit 780 is not limited to multiple, fixation kit 780 or one, an above-mentioned knot
The fixation kit 780 of structure also can be with the 715 common biological tissue clamped 20 of bottom wall of incubator 710；Fixation kit 780 is also unlimited
Directly the mounting portion 784a of fitting 784 can be mounted on and trained for example, fixing piece 782 can also omit in for above structure
On the bottom wall 715 for supporting case 710, at this point, mounting portion 784a is arranged to flexible, it is similar to the structure of telescopic rod, alternatively,
Mounting portion 784a can also can be slidably arranged on side wall 716.
Mobile station 800 can move, and mobile station 800 is fixedly connected with incubator, so that mobile station 800 can drive culture
Case moves.Specifically, incubator 710, liquid storage bottle 720, waste liquid bottle 740 and temperature control part 760 are installed in mobile station 800.Control
Device 100 processed can control the movement of mobile station 800.
Observation component 900 can acquire the image of the biological tissue 20 in incubator 710.Wherein, observation component 900 and control
Device 100 processed is electrically connected, and observation component 900 can be by image transmission to control device 100, and control device 100 can be according to shadow
As the movement of control mobile station 800.
Specifically, observation component 900 includes object lens 910 and light source 920, and object lens 910 can acquire the biology in incubator
The image of tissue 20, light source 920 can give object lens 910 to illuminate.Wherein, object lens 910 and light source 920 are electric with control device 100
Connection, object lens 910 can be by image transmission to control device 100.
The Mechanics Performance Testing of upper biological tissue 20 is carried out using the detecting system 10 of the mechanical property of above-mentioned biological tissue
Operation be specially：
The fixation of biological tissue 20：Also referring to Fig. 7, if biological tissue 20 is biologic soft tissue, feed liquor control is opened
Valve 730 makes the liquid in liquid storage bottle 720 pass through inlet tube 725 and flows into incubator 710, meanwhile, biological tissue 20 is placed in training
It supports in case 710, sliding and/or rotational installation portion 784a, so that the arc convex 784c of abutting part 784b and 20 phase of biological tissue
It abuts, biological tissue 20 is clamped between arc convex 784c and the bottom wall 715 of incubator 710, then nanometer pressure
The pressure head that trace tests system passes through instrument connection to carry out mechanical test to biological tissue 20, and liquid control valve is opened after the completion of test
750, so that the liquid in incubator 710 flows into waste liquid bottle 200.Since biologic soft tissue is not necessarily to culture solution culture, it is only necessary to phosphorus
Hydrochlorate buffer solution maintains the balance of structural constituent, it is therefore not necessary to be passed through carbon dioxide, without opening temperature control part 760,
Meanwhile without the update for keeping the liquid in incubator 710.
If biological tissue 20 is cell (being usually attached cell)：Temperature control part 760 is opened, so that the bottom wall of incubator 710
Temperature be 37 DEG C~37.5 DEG C, open liquid inlet control valve 730, the liquid in liquid storage bottle 720 passes through inlet tube 725 and flows into culture
In case 710, until the liquid in incubator 710 reaches required liquid level, liquid inlet control valve 730 turns the flow velocity of liquid down, goes out liquid
Control valve 750 is opened, and the liquid in incubator 710 is flowed by outlet tube 745 in waste liquid bottle 740, so as to have in incubator 710
It is liquid continuous to flow in and out, meanwhile, temperature control part 760 is opened, and the temperature in incubator 710 is made to maintain 37 DEG C~37.5 DEG C,
And carbon dioxide is passed through into incubator 710 by gas supply assembly 770, biological tissue 20 is placed in incubator 710, biological group
It knits 20 and is adhered on the bottom wall 715 of incubator 710 and grow, pass through instrument connection to biological group the pressure head of nano-indenter test system
Knit 20 carry out mechanical tests.Due to attached cell can adherent growth, it is therefore not necessary to be fixed using fixation kit 780.
Mobile mobile station 800 is opened so that the position of biological tissue 20 is corresponding with the position of pressure head and detection part 400
Driver 200 and to control piece 500 power, driver 200 to compression bar 310 apply to close to biological sample power so that compression bar
310 move with dynamic head and detection part 400 towards close to the direction of biological tissue 20, until detection part 400 connects with biological tissue 20
It touches, control piece 500 is made to power off, detection part 400 stops moving towards close to the direction of biological tissue 20, and driver 200 drives compression bar
310 band dynamic heads 320 at the uniform velocity move, and pressure head 320 squeezes biological tissue 20, and when detection part 400 and biological tissue 20 contact,
Displacement sensor 600 starts to detect the real-time displacement of compression bar 310, and the real-time displacement is transferred to control device 100, works as pressure head
320 are pressed into the predetermined depth of biological tissue 20, are maintained at predetermined depth for a period of time, and then driver 200 is applied to compression bar 310
Add a unloading force, so that pressure head 320 is moved towards the direction far from biological tissue 20, and pressure head 320 and biological tissue 20 is made to divide
From, alternatively, until driver 200 to compression bar 310 applies towards the power close to biological tissue 20 to pre set force, holding is to compression bar
310 apply pre set force to preset time, and driver 200 applies unloading force to compression bar 310 again, so that pressure head 320 is towards far from biology
The direction movement of tissue 20, and pressure head 320 and biological tissue 20 is made to detach.
The detecting system 10 of the mechanical property of above-mentioned biological tissue at least has the advantage that：
(1) since the detection part 400 of the detecting system 10 of the mechanical property of above-mentioned biological tissue can be with pressure head component 300
It is mobile, and detection part 400 can contact while pressure head component 300 is contacted with biological tissue 20 with biological tissue 20, displacement
Sensor 600 can start to detect the real-time displacement of pressure head component 300 when detection part 400 and biological tissue 20 contact, then,
It can relatively accurately know the time that pressure head component 300 is contacted with biological tissue 20 by detection part 400, and make displacement sensing
Device 600 can relatively accurately start to detect the real-time of pressure head component 300 when contacting at the beginning of pressure head component 300 and biological tissue 20
Displacement so that the real-time displacement that displacement sensor 600 detects is more nearly the practical indentation biological tissue 20 of pressure head component 300
Depth is conducive to the accuracy for improving detection data；Simultaneously as detection part 400 can also be when contacting with biological tissue 20
Stop moving with pressure head component 300, and so that pressure head component 300 is individually continued to move to squeeze biological tissue 20, thus
While realizing that pressure head component 300 squeezes biological tissue 20, prevent detection part 400 from continuing to move to squeeze with pressure head component 300
The accuracy of test, therefore, the measurement knot of the detecting system 10 of the mechanical property of above-mentioned biological tissue are influenced to biological tissue 20
Fruit is more accurate.
(2) above-mentioned sample fixing device 700 can contain the liquid storage bottle 720 of liquid by setting, and make liquid storage bottle 720 with
Inlet 712 is connected to, and the liquid in liquid storage bottle 720 can flow into incubator 710, and the liquid in incubator 710 can be from going out
Liquid mouth 714 flows out, then, during test, it can ensure that the liquid in incubator 710 in lasting update, is protected simultaneously
The nutritional need in the liquid in incubator 710 is demonstrate,proved, and the temperature in incubator 710 is controlled to ensure to train by temperature control part 760
The temperature requirements for supporting the biological tissue 20 in case 710, to enable biological tissue 20 in a more normal physiological environment
Lower carry out nano-indenter test avoids dehydration of the biological tissue 20 in detection process on traditional sample stage or gradually withers
The problem of test result inaccuracy caused by dying etc., therefore, above-mentioned sample fixing device 700 can make nano-indenter test
Test result is more accurate.
(3) by the way that mounting portion 784a is arranged to rotation, in order to the biological tissue 20 that fixation is different.
(4) setting gas supply assembly 770 to the biological tissue 20 in incubator 710 to supplement carbon dioxide, to maintain to cultivate
The balance of medium pH value in case makes biological tissue 20 (such as cell) that can be in a normal physiologic ring for a long time
Under border, the time-to-live of biological tissue 20 is improved due to increasing, and is conducive to the test of long period and improves the standard of test result
True property.
(5) usually biologic soft tissue can be suspended on liquid, and fixation kit 780 is arranged, and it is soft to can be used in fixed biology
Tissue, in order to the mechanical property of the 10 test biology soft tissue of detecting system of the mechanical property of biological tissue, on increasing
The purposes for stating sample fixing device 700 expands the use scope of above-mentioned sample fixing device 700.
(6) arc convex 784c is set on abutting part 784b, and arc convex 784c is made to be abutted against with biological tissue 20,
With the 715 common biological tissue clamped 20 of bottom wall with incubator 710, to reduce the bearing surface of fitting 784 and biological tissue 20
Product, improves the accuracy of test result.
It should be noted that liquid level gauge 735 can be omitted, at this point, liquid control valve 750 and liquid inlet control valve 730 can be with
It is controlled by program setting.Alternatively, liquid control valve 750 is not limited to solenoid valve, can also be common hand control valve
Or the adjustable fixture of clamping degree, at this point, manually controlling liquid control valve 750 as needed by operator to adjust out liquid
The flowing velocity of liquid in pipe 745；Liquid inlet control valve 730 is also not necessarily limited to as solenoid valve, or common is manual
Control valve or the adjustable fixture of clamping degree, at this point, manually controlling liquid inlet control valve 730 as needed by operator to adjust
The flowing velocity for saving the liquid in inlet tube 725, at this point, liquid level gauge 735 can be omitted.Or liquid control valve 750
With in liquid inlet control valve 730 can also one be solenoid valve, controlled by liquid level gauge 735, another be hand control valve or folder
The adjustable fixture of tight degree.
It is appreciated that liquid control valve 750 can be omitted, the flowing speed of the liquid in control outlet tube 745 may not need
Degree；Liquid inlet control valve 730 can also be omitted, the flowing velocity of the liquid in outlet tube 745.Waste liquid bottle 740 can also omit, this
When, the waste liquid that the liquid outlet 714 of incubator 710 flows out is flowed directly out to the external world.
If being appreciated that, biological tissue 20 is biologic soft tissue, it will usually be suspended on liquid, pass through fixation kit 780
It can be fixed, in order to the mechanical property of nano-indenter test biologic soft tissue, and since attached cell can be close to culture
On the inner wall of case 710, fixed without fixation kit 780, therefore, if the biological tissue 20 of test is attached cell, Gu
Determine component 780 can be omitted.
If being appreciated that, biological tissue 20 is biologic soft tissue, and gas supply assembly 770 can be omitted, and biological tissue 20 is not necessarily to
Carbon dioxide is provided.
The detection method of the mechanical property of the biological tissue 20 of embodiment one, to use the mechanical property of above-mentioned biological tissue
Detecting system 10 detect the detection method of biological tissue 20 mechanical property, in the present embodiment, biological tissue 20 is cell.Its
In, the detection method of the mechanical property of the biological tissue 20 includes the following steps：
Step S1：Mobile biological tissue 20, so that the position of biological tissue 20 and pressure head component 300 and detection part 400
Position is corresponding.
It should be noted that if the position of biological tissue 20 is just opposite with the position of pressure head component 300 and detection part 400
It answers, then step S1 can be omitted.
Step S2：Pressure head component 300 and detection part 400 is controlled to move towards close to the direction of biological tissue 20.
Specifically, it is moved towards close to the direction of biological tissue 20 using 200 drive ram component 300 of driver, meanwhile, control
Device 100 processed controls detection part 400 by control piece 500 and is fixed with pressure head component 300, so that detection part 400 is with pressure head component
300 move towards close to the direction of biological tissue 20.
Step S3：Until detection part 400 is contacted with biological tissue 20, control detection part 400 stops movement, wherein detection
Part 400 is contacted with biological tissue 20 simultaneously with pressure head component 300.
Specifically, when detection part 400 and biological tissue 20 contact, control device 100 controls control piece 500 by detection part
400 detach with pressure head component 300, so that detection part 400 stops movement.
Step S4：To 300 applied force of pressure head component, so that pressure head component 300 is moved towards close to the direction of biological tissue 20,
Until pressure head component 300 is pressed into biological tissue 20 to predetermined depth, keep pressure head component 300 predetermined depth stop to it is default when
Between, unloading force then is applied to pressure head component 300, so that pressure head component 300 is moved towards the direction far from biological tissue 20, and is made
Pressure head component 300 and biological tissue 20 detach, and record pressure head component 300 from detection part 400 and 20 contact start of biological tissue
Real-time displacement when being detached to pressure head component 300 and biological tissue 20 and the real-time force to the application of pressure head component 300.
Specifically, control device 100 controls driver 200 and is exerted a force to pressure head component 300 so that pressure head component 300 is towards close
The direction of biological tissue 20 is moved, and applies unloading force to pressure head component 300, so that pressure head component 300 is towards far from biological tissue
20 direction movement.Therefore, it is known quantity to the real-time force that pressure head component 300 applies.Predetermined depth is pressed into for pressure head component 300
The depth capacity of biological tissue 20, the real-time displacement of pressure head component 300 are the real-time displacement of pressure head 320, pass through displacement sensing
Device 600 can detect to obtain, and also be known quantity.
Step S5：The relation curve of power and displacement is established according to real-time displacement and real-time loading power.
Specifically, control device 100 establishes the relation curve of power and displacement according to real-time displacement and real-time loading power.
Step S6：The mechanical property of biological tissue 20 is calculated according to power and the relation curve of displacement.
Specifically, control device 100 calculates the mechanical property of biological tissue 20 according to power and the relation curve of displacement.
Wherein, the mechanical property of biological tissue 20 can be hardness, can also be elasticity modulus.
If mechanical property is hardness, it can be calculated and be obtained by following calculation formula (1)：
Wherein, H is the hardness of biological tissue 20, and P is adding when pressure head component 300 is pressed into biological tissue 20 to predetermined depth
Power is carried, is known quantity；AcIt is pressure head 320 and the contact area function of biological tissue 20,C0、C1、C2、C3、C4And C5The school of equal contact area function
Positive coefficient is known quantity.hcIt is the practical compression distance that pressure head 320 is pressed into biological tissue 20,
hmaxIt is the depth capacity that pressure head 320 is pressed into biological tissue 20, i.e. predetermined depth, is known quantity；S is contact stiffness, andFor to pressure head component 300 to applying unloading force (even if pressure head component 300 is towards the side far from biological tissue 20
To mobile unloading force) after the relation curve fitting of power and displacement when starting to pressure head component 300 and biological tissue 20 to detach
The derivation of obtained functional relation；F is unloading force, this power is the power that driver 200 applies, and is known quantity；H is to press head group
Part 300 starts to applying unloading force (even if unloading force that pressure head component 300 is moved towards the direction far from biological tissue 20) to pressure
Displacement when head assembly 300 and biological tissue 20 detach can be obtained by the detection of displacement sensor 600, be known quantity.
When the mechanical property of calculating is elasticity modulus, calculation formula (2) is as follows：
Wherein, E is the elasticity modulus of biological tissue 20, AcWith the meaning of S respectively with the A during above-mentioned hardness calculationcWith
The meaning of S is identical, and π is pi, is known quantity.
Further, during step S1~step S4, biological tissue 20 is in the ring that temperature is 37 DEG C~37.5 DEG C
Under border and in the liquid of flow regime.Wherein, liquid is cell culture fluid or phosphate buffered saline solution, and biological tissue 20 is thin
Born of the same parents.Liquid ensures the nutritional need in liquid in lasting update, and temperature environment meets the temperature requirements of biological tissue 20,
To enable biological tissue 20 to carry out nano-indenter test under a more normal physiological environment, biological tissue is avoided
20 in detection process on traditional sample stage dehydration or caused bys gradual apoptosis etc. test result inaccuracy problem,
Further, during step S1~step S4, the product hundred of carbon dioxide body has also been passed through to biological tissue 20
It is 5% air to divide content.Make biology to give biological tissue 20 to supplement carbon dioxide to maintain the balance of the pH value in liquid
Tissue 20 can be under a normal physiological environment for a long time, when due to increasing the survival for improving biological tissue 20
Between, be conducive to the test of long period and improve the accuracy of test result.
The detection method of the mechanical property of the biological tissue 20 of embodiment one is easy to operate, and can make measurement result more
It is accurate.
The detection method of the mechanical property of the biological tissue 20 of embodiment two, the mechanical property for detecting biologic soft tissue
Can, roughly the same with the detection method of mechanical property of biological tissue 20 of embodiment one, difference lies in the biologies of embodiment two
The detection method of mechanical property of tissue 20 further includes before the step S1 of embodiment one：Biological tissue 20 is fixed on liquid
In, wherein liquid is phosphate buffered saline solution, and biological tissue 20 is soft tissue.Since biologic soft tissue is not necessarily to culture solution culture,
Phosphate buffer solution is only needed to maintain the balance of structural constituent, it is therefore not necessary to carbon dioxide is passed through, without holding liquid
Update.
The detection side of the detection method of the mechanical property of the biological tissue 20 of embodiment two and the mechanical property of embodiment one
Method is roughly the same, therefore also has the effect of that the detection method of the mechanical property of embodiment one is similar.
The mechanics of the detection method of the mechanical property of the biological tissue 20 of embodiment three and the biological tissue 20 of embodiment one
The detection method of performance is roughly the same, differs only in the difference of step S4, and in the present embodiment, which is：To press head group
Part exerts a force, so that pressure head component is moved towards close to the direction of biological tissue, and is pressed into biological tissue, until being applied to pressure head component
The power added reaches pre set force, keeps applying pre set force to preset time to pressure head component, then applies unloading force to pressure head component,
So that pressure head component is moved towards the direction far from biological tissue, and pressure head component and biological tissue is made to detach, and records press head group
Part since detection part with when being detached to pressure head component and biological tissue contact biological tissue real-time displacement and to pressure head component
The real-time force of application.
And the calculation formula of the mechanical property of the biological tissue of the present embodiment is also roughly the same with embodiment one, difference exists
In P is pre set force, is known quantity；hmaxIt is the depth capacity of pressure head indentation biological tissue, that is, starts to unload pressure head component application
The depth of pressure head indentation biological tissue, can be obtained by displacement sensor detection when carrying power, be known quantity.
The detection method of the detection method of the mechanical property of the biological tissue of embodiment three and the mechanical property of embodiment one
It is roughly the same, therefore also have the effect of that the detection method of the mechanical property of embodiment one is similar.
Each technical characteristic of embodiment described above can be combined arbitrarily, to keep description succinct, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, it is all considered to be the range of this specification record.
Above-described embodiments merely represent several embodiments of the utility model, the description thereof is more specific and detailed,
But therefore it can not be interpreted as the limitation to utility model patent range.It should be pointed out that for the common skill of this field
For art personnel, without departing from the concept of the premise utility, various modifications and improvements can be made, these are belonged to
The scope of protection of the utility model.Therefore, the protection domain of the utility model patent should be determined by the appended claims.
Claims (17)
1. a kind of detecting system of the mechanical property of biological tissue, the mechanical property for detecting biological tissue, which is characterized in that
Including：
Driver；
The pressure head component of the biological tissue can be squeezed, can be moved, the pressure head component is sequentially connected with the driver,
So that the driver can drive the pressure head component to move；
Detection part can be moved with the pressure head component, and can be in the pressure head component and the contact biological tissue
Simultaneously with the contact biological tissue, the detection part can also the detection part and stop when the contact biological tissue with
The pressure head component movement, and so that the pressure head component is individually continued to move to squeeze the biological tissue；And
Displacement sensor can detect the displacement of the pressure head component movement, and institute's displacement sensors can be in the detection
Start to detect the real-time displacement of the pressure head component when part is with the contact biological tissue.
2. the detecting system of the mechanical property of biological tissue according to claim 1, which is characterized in that the detection part energy
Enough to be fixed with the pressure head component, so that the detection part can be moved with the pressure head component, the detection part can also be with
The pressure head component separation, so that the detection part can stop moving with the pressure head component.
3. the detecting system of the mechanical property of biological tissue according to claim 2, which is characterized in that further include that can control
The control piece fixing and detach of the detection part with the pressure head component is made, the control piece can make the spy when being powered
It surveys part to fix with the pressure head component, the detection part is made to be detached with the pressure head component when power is off.
4. the detecting system of the mechanical property of biological tissue according to claim 1, which is characterized in that the detection part is
Tubular, the pressure head component include compression bar and pressure head, and the compression bar can move, and the pressure head is fixedly connected with the compression bar,
So that the pressure head can be moved with the compression bar, and the pressure head can be contained in the detection part, and the pressure head can also
The detection part is enough stretched out, when the pressure head is contained in the detection part, side and institute of the pressure head far from the compression bar
It is concordant to state detection part so that the pressure head and the detection part can simultaneously with the contact biological tissue, and the detection part
When with the contact biological tissue, the detection part stops moving with the compression bar, and the compression bar is enable individually to drive institute
It states pressure head to continue to move to, so that the pressure head can stretch out the detection part and squeeze the biological tissue.
5. the detecting system of the mechanical property of biological tissue according to claim 1, which is characterized in that further include that sample is solid
Determine device, the sample fixing device includes：
Incubator can contain the biological tissue, inlet and liquid outlet are offered on the incubator；
The liquid storage bottle that liquid can be contained is connected to the inlet, and the liquid in the liquid storage bottle can flow into the culture
In case, wherein the liquid in the incubator can be flowed out from the liquid outlet；
Temperature control part can control the temperature in the incubator.
6. the detecting system of the mechanical property of biological tissue according to claim 5, which is characterized in that further include feed liquor
Pipe, one end of the inlet tube are connected to the liquid storage bottle, and the other end is connected to the inlet, and the inlet tube is close to described
The height of one end of liquid storage bottle is higher than the inlet tube close to the height of one end of the inlet.
7. the detecting system of the mechanical property of biological tissue according to claim 6, which is characterized in that further include being mounted on
Liquid inlet control valve on the inlet tube, the liquid inlet control valve can control the flowing velocity of the liquid in the inlet tube.
8. the detecting system of the mechanical property of biological tissue according to claim 7, which is characterized in that further include being mounted on
Liquid level gauge in the incubator, the liquid level gauge can detect the level value in the incubator, the liquid inlet control valve energy
Enough flowing velocities that the liquid in the inlet tube is controlled according to the level value.
9. the detecting system of the mechanical property of biological tissue according to claim 5, which is characterized in that further include with it is described
The waste liquid bottle of liquid outlet connection, the liquid in the incubator can be flowed by the liquid outlet in the waste liquid bottle.
10. the detecting system of the mechanical property of biological tissue according to claim 5, which is characterized in that further include one end
The outlet tube being connected to the liquid outlet and the liquid control valve on the outlet tube, the liquid control valve can be controlled
Make the flowing velocity of the liquid in the outlet tube.
11. the detecting system of the mechanical property of biological tissue according to claim 5, which is characterized in that further include installation
Fixation kit in the incubator, the fixation kit include fitting, and the fitting can be with the biological tissue
It abuts against, so that the biological tissue can be clamped and fixed between the fitting and the inner wall of the incubator.
12. the detecting system of the mechanical property of biological tissue according to claim 11, which is characterized in that the incubator
With bottom wall, the fitting can be slided towards the direction of the bottom wall closer or far from the incubator, and the fitting energy
Enough to be abutted against with the biological tissue, the fitting can grip the biological group jointly with the bottom wall of the incubator
It knits.
13. the detecting system of the mechanical property of biological tissue according to claim 12, which is characterized in that described fixed group
Part further includes the fixing piece being fixed in the incubator, and the fitting includes rod-shaped mounting portion and from the mounting portion
One end bending extend bar shaped abutting part, the mounting portion be mounted on the fixing piece on, and can towards closer or far from
The direction of the incubator bottom wall is slided, and the mounting portion can also be rotated relative to the fixing piece, and the abutting part can be with
The biological tissue abuts against.
14. the detecting system of the mechanical property of biological tissue according to claim 13, which is characterized in that the abutting part
It is equipped with arc convex, the arc convex can be abutted against with the biological tissue.
15. the detecting system of the mechanical property of biological tissue according to claim 5, which is characterized in that further include gas supply
Component, the gas supply assembly are connected to the incubator, and the gas supply assembly can input carbon dioxide into the incubator.
16. the detecting system of the mechanical property of biological tissue according to claim 5, which is characterized in that further include observation
Component and can movably mobile station, the observation component can acquire the image of the biological tissue in the incubator,
The mobile station is fixedly connected with the incubator, and the mobile station can drive the incubator to move according to the image.
17. the detecting system of the mechanical property of biological tissue according to claim 16, which is characterized in that the observation group
Part includes object lens and light source, and the object lens can acquire the image of the biological tissue in the incubator, the light source energy
It is enough to be illuminated to the object lens.
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