CN207937460U - Sample fixing device and nanoindentation test system - Google Patents
Sample fixing device and nanoindentation test system Download PDFInfo
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- CN207937460U CN207937460U CN201721909376.XU CN201721909376U CN207937460U CN 207937460 U CN207937460 U CN 207937460U CN 201721909376 U CN201721909376 U CN 201721909376U CN 207937460 U CN207937460 U CN 207937460U
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- 238000012360 testing method Methods 0.000 title claims abstract description 48
- 239000007788 liquid Substances 0.000 claims abstract description 157
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 16
- 239000002699 waste material Substances 0.000 claims description 11
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 8
- 239000001569 carbon dioxide Substances 0.000 claims description 8
- 238000001514 detection method Methods 0.000 claims description 5
- 238000005452 bending Methods 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 description 19
- 239000000047 product Substances 0.000 description 18
- 210000004872 soft tissue Anatomy 0.000 description 11
- 210000001519 tissue Anatomy 0.000 description 6
- 238000010586 diagram Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 230000006907 apoptotic process Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000001464 adherent effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000012930 cell culture fluid Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000018044 dehydration Effects 0.000 description 2
- 238000006297 dehydration reaction Methods 0.000 description 2
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 2
- 239000013013 elastic material Substances 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000002953 phosphate buffered saline Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241000224016 Plasmodium Species 0.000 description 1
- 230000002917 arthritic effect Effects 0.000 description 1
- 210000001188 articular cartilage Anatomy 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 210000003321 cartilage cell Anatomy 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000008055 phosphate buffer solution Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000007779 soft material Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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Abstract
The utility model relates to a sample fixing device and nanoindentation test system. The sample fixing device comprises an incubator, a liquid storage bottle and a temperature control piece, wherein the incubator can be used for containing a sample to be detected, and the incubator is provided with a liquid inlet and a liquid outlet; the liquid storage bottle can contain liquid, the liquid storage bottle is communicated with the liquid inlet, the liquid in the liquid storage bottle can flow into the incubator, and the liquid in the incubator can flow out of the liquid outlet; the temperature control piece can control the temperature in the incubator. The sample fixing device can enable the test result of the nanoindentation test to be more accurate.
Description
Technical field
The utility model is related to biotechnology more particularly to a kind of sample fixing device and nano-indenter test systems
System.
Background technology
In the pathogenic process of many diseases, it is usually associated with the change of the mechanical property for the basic unit for constituting biological tissue
Change.Such as, cancer cell is softer than normal body cell, has stronger deformability；The erythrocyte of plasmodium is infected than normal
Erythrocyte it is hard；Arthritic cartilage cell is softer than normal articular cartilage cell.Therefore, by measuring biological tissue or cell
The generation of lesion also can be predicted in mechanical property under micro-nano-scale.
Nano-indenter test instrument have high resolution, compression distance it is shallow, not lesioned sample surface, to sample shape and size
The advantages that without particular/special requirement, therefore, nano-indenter test instrument, become the mechanical properties of soft materials such as research cell, biological tissue
Effective tool.Sample stage currently used for placing nano-indenter test is usually the cylindrical sample platform of Keysight (Agilent)
And the suction magnet fixing device of Hysitron, and the mechanical property of the viscous-elastic materials such as biological tissue and cell with its residing for
Test environment is closely related, is tested if the viscous-elastic materials such as its biological tissue and cell are placed on above-mentioned sample stage, can cause
Sample is dehydrated, and is resulted even in the gradual apoptosis of cell, is seriously affected the accuracy of test result.
Utility model content
Based on this, it is necessary to which providing a kind of can make the more accurate sample of the test result of nano-indenter test fix dress
It sets.
In addition, also providing a kind of nano-indenter test system.
A kind of sample fixing device, including：
Incubator can contain detected sample, inlet and liquid outlet are offered on the incubator；
The liquid storage bottle that liquid can be contained is connected with the inlet, and the liquid in the liquid storage bottle can flow into institute
It states in incubator, wherein the liquid in the incubator can be flowed out from the liquid outlet；And
Temperature control part can control the temperature in the incubator.
Above-mentioned sample fixing device can contain the liquid storage bottle of liquid by setting, and liquid storage bottle is made to be connected with inlet
Logical, the liquid in liquid storage bottle can flow into incubator, and the liquid in incubator can be flowed out from liquid outlet, then, it is surveying
It during examination, can ensure the liquid continuous update in incubator simultaneously, ensure the nutritional need of the liquid in incubator,
And by the temperature in temperature control part control incubator to ensure the temperature requirements of the detected sample in incubator, it is to be checked to make
Sample can carry out nano-indenter test under a more normal physiological environment, avoid detected sample and detecting
In journey on traditional sample stage dehydration or caused bys gradual apoptosis etc. test result inaccuracy problem, it is therefore, above-mentioned
Sample fixing device can make the test result of nano-indenter test more accurate.
Further include inlet tube in one of the embodiments, one end of the inlet tube is connected with the liquid storage bottle, separately
One end is connected with the inlet, and height of the inlet tube close to one end of the liquid storage bottle is close higher than the inlet tube
The height of one end of the inlet.
Further include the feed liquor control piece being mounted on the inlet tube in one of the embodiments, the feed liquor control
Part can control the flowing velocity of the liquid in the inlet tube.
Further include the liquid level gauge being mounted in the incubator in one of the embodiments, the liquid level gauge can be examined
The liquid level in the incubator is surveyed, the feed liquor control piece can be according to the stream of the liquid in inlet tube described in the Liquid level
Dynamic speed.
Further include the waste liquid bottle being connected with the liquid outlet, the liquid in the incubator in one of the embodiments,
Body can be flowed by the liquid outlet in the waste liquid bottle.
Further include in one of the embodiments, outlet tube that one end is connected with the liquid outlet and mounted on it is described go out
Go out hydraulic control product in liquid pipe, it is described go out hydraulic control product can control the flowing velocity of the liquid in the outlet tube.
Further include the fixation kit being mounted in the incubator, the fixation kit packet in one of the embodiments,
Fitting is included, the fitting can be abutted against with the detected sample, so that the detected sample can be clamped admittedly
It is scheduled between the fitting and the inner wall of the incubator.
The incubator has bottom wall in one of the embodiments, and the fitting can be towards closer or far from described
The direction of incubator bottom wall is slided, and the fitting can be abutted against with the detected sample, and the fitting can be with
The bottom wall of the incubator grips the detected sample jointly.
The fixation kit further includes the fixing piece being fixed in the incubator in one of the embodiments, described
Fitting includes the abutting part of rod-shaped mounting portion and the bar shaped extended from the bending of one end of the mounting portion, the mounting portion peace
It on the fixing piece, and can be slided towards the direction closer or far from the incubator bottom wall, the mounting portion can also
Enough to be rotated relative to the fixing piece, the abutting part can be abutted against with the detected sample.
The abutting part is equipped with arc convex in one of the embodiments, and the arc convex can be waited for described
Detection sample abuts against.
Further include gas supply assembly in one of the embodiments, the gas supply assembly is connected with the incubator, described
Gas supply assembly can input carbon dioxide into the incubator.
A kind of nano-indenter test system, including above-mentioned sample fixing device.
Description of the drawings
Fig. 1 is the structural schematic diagram of the sample fixing device of an embodiment；
Fig. 2 is that the structure that incubator, fixation kit and the liquid level gauge of sample fixing device shown in FIG. 1 fit together is shown
It is intended to；
Fig. 3 is the incubator of sample fixing device shown in FIG. 1 and fixation kit fits together and incubator is omitted
The structural schematic diagram of another angle of roof；
Fig. 4 is the structural schematic diagram of fixation kit shown in 2；
Incubator and fixation kit that Fig. 5 is Fig. 2 are fixed with the structural schematic diagram of detected sample.
Specific implementation mode
The utility model is more fully retouched below with reference to relevant drawings for the ease of understanding the utility model,
It states.The preferred embodiment of the utility model is given in attached drawing.But the utility model can come in many different forms
It realizes, however it is not limited to embodiment described herein.Make to the utility model on the contrary, purpose of providing these embodiments is
The understanding of disclosure is more thorough and comprehensive.
It should be noted that when element is referred to as " being fixed on " another element, it can be directly on another element
Or there may also be elements placed in the middle.When an element is considered as " connection " another element, it can be directly connected to
To another element or it may be simultaneously present centering elements.Term as used herein " vertical ", " horizontal ", " left side ",
" right side " and similar statement are for illustrative purposes only.
Unless otherwise defined, all of technologies and scientific terms used here by the article is led with the technology for belonging to the utility model
The normally understood meaning of technical staff in domain is identical.Terminology used in the description of the utility model herein only be
The purpose of description specific embodiment, it is not intended that in limitation the utility model.
As shown in Figure 1, the nano-indenter test system (figure is not marked) of an embodiment, can test the power of detected sample
Performance is learned, for example, elasticity modulus, hardness etc..Detected sample is the glutinous bullets such as biologic soft tissue, cell (being usually attached cell)
The sample of property.The nano-indenter test system includes sample fixing device 10, and the sample fixing device 10 is for containing and fixing
Detected sample, and make detected sample during detection under a more normal physiological environment, it is surveyed with improving
The accuracy of test result.
Specifically in the illustrated embodiment, which includes incubator 100, liquid storage bottle 200, feed liquor control
Part 300, waste liquid bottle 400, goes out hydraulic control product 500, temperature control part 600, gas supply assembly 700 and fixation kit 800 at liquid level gauge 350.
Incubator 100 can contain detected sample, and inlet 110 and liquid outlet 120 are offered on incubator 100.Its
In, liquid can be added from inlet 110 in incubator 100；Liquid in incubator 100 can be flowed out from liquid outlet 120.It should
Liquid is cell culture fluid or phosphate buffered saline solution, if detected sample is cell, which is usually cell culture fluid,
If detected sample is biologic soft tissue, liquids in general is phosphate buffered saline solution.
Also referring to Fig. 2, specifically, incubator 100 includes bottom wall 130, side wall 140 and roof 150,140 ring of side wall
Continuously it is arranged one week around bottom wall 130, to form the accommodating space that can house detected sample, roof 150 is covered on side wall 140
On side far from bottom wall 130, and accommodating space is covered, the pressure head offered on roof 150 for nano-indenter test system is worn
If instrument connection.Wherein, inlet 110 and liquid outlet 120 are opened on the side wall 140 of incubator 100.Inlet 110 arrives
The height of bottom wall 130 arrives the height of bottom wall 130 higher than liquid outlet 120.Specifically in the illustrated embodiment, incubator 100 is substantially
For open column shape structure.
Liquid storage bottle 200 can contain liquid, and liquid storage bottle 200 is connected with inlet 110, the liquid energy in liquid storage bottle 200
Enough flow into incubator 100.Specifically, sample fixing device 10 further includes inlet tube 250, one end of inlet tube 250 and liquid storage
Bottle 200 is connected, and the other end is connected with inlet 110, to realize the connection of liquid storage bottle 200 and incubator 100.
Further, height of the inlet tube 250 close to one end of liquid storage bottle 200 is higher than inlet tube 250 close to inlet 110
One end height so that liquid in liquid storage bottle 200 can be flowed into voluntarily by inlet tube 250 in incubator 100, and nothing
Driving device need to be set.
It should be noted that height of the inlet tube 250 close to one end of liquid storage bottle 200 is not limited to lean on higher than inlet tube 250
The height of one end of nearly inlet 110, can also be arranged to identical height, either, inlet tube 250 is close to liquid storage bottle 200
One end height less than inlet tube 250 close to the height of one end of inlet 110, at this point, setting driving device, such as water
Pump can also realize that the liquid in liquid storage bottle 200 can be flowed by inlet tube 250 in incubator 100.
Feed liquor control piece 300 is mounted on inlet tube 250, and feed liquor control piece 300 can control the liquid in inlet tube 250
Body flows into the speed in incubator 100.Specifically, feed liquor control piece 300 is solenoid valve.
Liquid level gauge 350 is mounted in incubator 100, and liquid level gauge 350 can detect the liquid level in incubator 100, into hydraulic control
Product 300 can flow into the flowing velocity in incubator 100 according to the liquid in Liquid level inlet tube 250.For example, when culture
When liquid level in case 100 does not reach required liquid level, feed liquor control piece 300 controls the liquid in inlet tube 250 with maximum flow rate
It is flowed into incubator 100, so that incubator 100 can reach required liquid level in the short time, the liquid in incubator 100
When position reaches required liquid level, it is zero that feed liquor control piece 300, which controls the flow velocity reduction of the liquid in inlet tube 250 or coutroi velocity,.
Waste liquid bottle 400 is connected with liquid outlet 120, and the liquid in incubator 100 can be flowed into useless by liquid outlet 120
In liquid bottle 400.Specifically, sample fixing device 10 further includes outlet tube 450, one end and 120 phase of liquid outlet of outlet tube 450
Connection, the other end is connected with waste liquid bottle 400, to realize the connection of waste liquid bottle 400 and incubator 100.
Go out hydraulic control product 500 on the outlet tube 450, liquid in outlet tube 450 can be controlled by going out hydraulic control product 500
The rate of outflow of body.Specifically, it is solenoid valve to go out hydraulic control product 500, and going out hydraulic control product 500 can be according to Liquid level outlet tube
The flowing velocity of liquid in 450.For example, when the liquid level in incubator 100 does not reach required liquid level, go out hydraulic control product
The flow velocity of liquid in 500 control outlet tubes 450 is zero, so that incubator 100 can reach required liquid level in the short time, when
When liquid level in incubator 100 reaches required liquid level, goes out the liquid that hydraulic control product 500 controls in inlet tube 250 and flow, in order to
Liquid in incubator 100 being capable of constant flow.
Temperature control part 600 can control the temperature in incubator 100 so that the temperature in incubator 100 maintain 37 DEG C~
37.5℃.Specifically, temperature control part 600 is arranged in the outside of incubator 100, and the bottom wall 130 of temperature control part 600 and incubator 100
It is fixedly connected.
Gas supply assembly 700 is connected with incubator 100, and gas supply assembly 700 can input carbon dioxide into incubator 100
Volumn concentration be 5% air, in incubator 100 detected sample supplement carbon dioxide, maintain incubator
The pH value of middle culture solution is in normal range (NR), so that detected sample (such as cell) can be in one normally for a long time
Under physiological environment, increase the time-to-live for improving detected sample, be conducive to the test of long period and improve test result
Accuracy.
Detected sample (such as biologic soft tissue) can be fixed in incubator 100 by fixation kit 800, to prevent to wait for
Detection sample slides in incubator 100 so that above-mentioned sample fixing device 10 also can be used in the sample of non-adherent cell
Nano-indenter test.According to Saint Venant's principle, stress and Deformation Field are locally only generated to the pressing of detected sample, pressed separate
The result that intermediate pressure section carries out nanometer mechanics measurement will be unaffected, therefore, by the way that fixation kit 800 and detected sample is arranged
Part abuts against, and can not only realize the fixation of detected sample, and on test result also without influence.
Also referring to Fig. 3 and Fig. 4, specifically, fixation kit 800 is multiple, the interval of multiple fixation kits 800 setting
On the bottom wall 130 of incubator 100, and multiple fixation kits 800 are concyclic, the bottom wall of multiple fixation kits 800 and incubator 100
130 can be clamped detected sample jointly.Wherein, each fixation kit 800 includes fixing piece 810 and fitting 820.
Fixing piece 810 is fixed in incubator 100.Specifically, fixing piece 810 is fixed on the bottom wall 130 of incubator 100
On.More specifically, 810 generally cylindrical structure of fixing piece, one end of fixing piece 810 is fixed with the bottom wall 130 of incubator 100 to be connected
It connects, the other end is extended vertically with respect to the bottom wall 130 of incubator 100.
It should be noted that fixing piece 810 is not limited to be fixed on the bottom wall 130 of incubator 100, it is also fixed on incubator
On 100 side wall 140.
Fitting 820 can be abutted against with detected sample, so that detected sample can be clamped and fixed in fitting
Between 820 and the inner wall of incubator 100.Specifically, fitting 820 is movably mounted on fixing piece 810.Wherein, fitting
820 can slide towards the direction of the bottom wall 130 closer or far from incubator 100, and fitting 820 can be with detected sample phase
It abuts, fitting 820 can grip detected sample jointly with the bottom wall 130 of incubator 100.
More specifically, fitting 820 bends the item extended including rod-shaped mounting portion 822 and from one end of mounting portion 822
The abutting part 824 of shape, mounting portion 822 are mounted on fixing piece 810, and can be towards the bottom wall closer or far from incubator 100
130 direction sliding, mounting portion 822 can also opposed fixed member 810 rotate, and abutting part 824 can be with detected sample phase
It abuts.I.e. abutting part 824 can jointly coordinate with the bottom wall 130 of incubator 100 grips detected sample.Specifically, pacify
The setting coaxial with fixing piece 810 of dress portion 822, mounting portion 822 being capable of 360 ° of rotations of the generation of opposed fixed member 810.
Further, abutting part 824 is equipped with arc convex 826, and arc convex 826 can offset with detected sample
Connect, i.e., arc convex 826 detected sample can be clamped jointly with the bottom wall 130 of incubator 100, with reduce fitting 820 with
The contact area of detected sample improves the accuracy of test result.Specifically in the illustrated embodiment, arc convex 826
In the one end of abutting part 824 far from mounting portion 822.
Specifically, the mounting portion 822 of fitting 820 and 810 screw-thread fit of fixing piece, to by by fitting 820
Mounting portion 822 and fixing piece 810, which screw togather, can simply implement between abutting part 824 and the bottom wall 130 of incubator 100
The adjusting of distance, and realize the rotation of 822 opposed fixed member 810 of mounting portion.
It should be noted that fixation kit 800 is not limited to multiple, fixation kit 800 or one, an above-mentioned knot
The fixation kit 800 of structure also detected sample can be clamped jointly with the bottom wall 130 of incubator 100；Fixation kit 800 is also unlimited
Directly the mounting portion 822 of fitting 820 can be mounted on and trained for example, fixing piece 810 can also omit in for above structure
On the bottom wall 130 for supporting case 100, at this point, mounting portion 822 is arranged to flexible, it is similar to the structure of telescopic rod, alternatively,
Mounting portion 822 can also can be slidably arranged on side wall 140.
Using above-mentioned sample fixing device 10 carry out nano-indenter test operation be specially：
If detected sample is cell (being usually attached cell)：Open feed liquor control piece 300, the liquid in liquid storage bottle 200
Body is flowed by inlet tube 250 in incubator 100, until the liquid in incubator 100 reaches required liquid level, feed liquor control piece
300 turn the flow velocity of liquid down, go out the opening of hydraulic control product 500, and passing through outlet tube 450 in incubator 100 flows into waste liquid bottle 400
In so that have in incubator 100 it is liquid continuous flow in and out, meanwhile, temperature control part 600 open, make the temperature in incubator 100
37 DEG C~37.5 DEG C are maintained, and carbon dioxide is passed through into incubator 100 by gas supply assembly 700, detected sample is set
In incubator 100, detected sample, which is adhered on the bottom wall 130 of incubator 100, to be grown, by the pressure of nano-indenter test system
Head passes through instrument connection to carry out mechanical test to detected sample.Due to attached cell can adherent growth, it is therefore not necessary to using solid
Determine the fixation of component 800.
Please refer to fig. 5, if test sample to be checked 20 is biologic soft tissue：Feed liquor control piece 300 is opened, liquid storage bottle 200 is made
In liquid by inlet tube 250 flow into incubator 100 in, meanwhile, detected sample 20 is placed in incubator 100, slide
And/or rotational installation portion 822, so that the arc convex 826 of abutting part 824 is abutted against with detected sample 20, it will be to be detected
Sample 20 is clamped between arc convex 826 and the bottom wall 130 of incubator 100, then the pressure of nano-indenter test system
Head passes through instrument connection to carry out mechanical test to detected sample 20, hydraulic control product 500 is opened out after the completion of test, so that incubator
Liquid in 100 flows into waste liquid bottle 200.Since biologic soft tissue is not necessarily to culture solution culture, it is only necessary to which phosphate buffer solution is tieed up
The balance of structural constituent is held, it is therefore not necessary to it is passed through carbon dioxide, without opening temperature control part 600, meanwhile, without guarantor
Hold the update of the liquid in incubator 100.
Above-mentioned sample fixing device 10 at least has the advantage that：
(1) above-mentioned sample fixing device 10 can contain the liquid storage bottle 200 of liquid by setting, and make liquid storage bottle 200 with
Inlet 110 is connected, and the liquid in liquid storage bottle 200 can flow into incubator 100, and the liquid in incubator 100 can be from
Liquid outlet 120 flows out, then, during test, it can ensure liquid in incubator 100 simultaneously in lasting update,
Ensure the nutritional need in the liquid in incubator 100, and the temperature in incubator 100 is controlled to ensure by temperature control part 600
The temperature requirements of detected sample in incubator 100, to enable detected sample in a more normal physiologic ring
Nano-indenter test is carried out under border, avoids dehydration or gradual of the detected sample in detection process on traditional sample stage
The problem of test result inaccuracy caused by apoptosis etc., therefore, above-mentioned sample fixing device 10 can make nano-indenter test
Test result it is more accurate.
(2) by the way that mounting portion 822 is arranged to rotation, in order to the detected sample that fixation is different.
(3) setting gas supply assembly 700 to the detected sample in incubator 100 to supplement carbon dioxide, to maintain to cultivate
The balance of medium pH value in case makes detected sample (such as cell) that can be in a normal physiologic ring for a long time
Under border, the time-to-live of detected sample is improved due to increasing, and is conducive to the test of long period and improves the standard of test result
True property.
(4) usually biologic soft tissue can be suspended on liquid, and fixation kit 800 is arranged, and it is soft to can be used in fixed biology
Tissue, in order to the mechanical property of nano-indenter test biologic soft tissue, to increase the purposes of above-mentioned sample fixing device 10,
Expand the use scope of above-mentioned sample fixing device 10.
(5) arc convex 826 is set on abutting part 824, and arc convex 826 is made to be abutted against with detected sample, and
Detected sample is clamped jointly with the bottom wall 130 of incubator 100, to reduce the bearing surface of fitting 820 and detected sample
Product, improves the accuracy of test result.
It should be noted that liquid level gauge 350 can be omitted, at this point, going out hydraulic control product 500 and feed liquor control piece 300 can be with
It is controlled by program setting.Alternatively, going out hydraulic control product 500 is not limited to solenoid valve, can also be common hand control valve
Or the adjustable fixture of clamping degree, at this point, manually controlling hydraulic control product 500 as needed by operator adjusts out liquid
The flowing velocity of liquid in pipe 450；Feed liquor control piece 300 is also not necessarily limited to as solenoid valve, or common is manual
Control valve or the adjustable fixture of clamping degree, at this point, manually controlling feed liquor control piece 300 as needed by operator to adjust
The flowing velocity for saving the liquid in inlet tube 250, at this point, liquid level gauge 350 can be omitted.Or go out hydraulic control product
500 and feed liquor control piece 300 in can also one be solenoid valve, controlled by liquid level gauge 350, another for hand control valve or
The adjustable fixture of clamping degree.
It can be omitted it is appreciated that going out hydraulic control product 500, may not need the flowing speed of the liquid in control outlet tube 450
Degree；Feed liquor control piece 300 can also omit, the flowing velocity of the liquid in outlet tube 450.Waste liquid bottle 400 can also omit, this
When, the waste liquid that the liquid outlet 120 of incubator 100 flows out is flowed directly out to the external world.
If being appreciated that, detected sample is biologic soft tissue, it will usually be suspended on liquid, pass through fixation kit 800
It can be fixed, in order to the mechanical property of nano-indenter test biologic soft tissue, and since attached cell can be close to culture
On the inner wall of case 100, fixed without fixation kit 800, therefore, if the detected sample of test is attached cell, Gu
Determine component 800 can be omitted.
If being appreciated that, detected sample is biologic soft tissue, and gas supply assembly 700 can be omitted, and biologic soft tissue is not necessarily to
Carbon dioxide is provided.
Each technical characteristic of embodiment described above can be combined arbitrarily, to keep description succinct, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, it is all considered to be the range of this specification record.
Above-described embodiments merely represent several embodiments of the utility model, the description thereof is more specific and detailed,
But therefore it can not be interpreted as the limitation to utility model patent range.It should be pointed out that for the common skill of this field
For art personnel, without departing from the concept of the premise utility, various modifications and improvements can be made, these are belonged to
The scope of protection of the utility model.Therefore, the protection domain of the utility model patent should be determined by the appended claims.
Claims (12)
1. a kind of sample fixing device, which is characterized in that including：
Incubator can contain detected sample, inlet and liquid outlet are offered on the incubator；
The liquid storage bottle that liquid can be contained is connected with the inlet, and the liquid in the liquid storage bottle can flow into the training
It supports in case, wherein the liquid in the incubator can be flowed out from the liquid outlet；And
Temperature control part can control the temperature in the incubator.
2. sample fixing device according to claim 1, which is characterized in that further include inlet tube, the one of the inlet tube
End is connected with the liquid storage bottle, and the other end is connected with the inlet, and the inlet tube is close to one end of the liquid storage bottle
Height higher than the inlet tube close to one end of the inlet height.
3. sample fixing device according to claim 2, which is characterized in that further include be mounted on the inlet tube on into
Hydraulic control product, the feed liquor control piece can control the flowing velocity of the liquid in the inlet tube.
4. sample fixing device according to claim 3, which is characterized in that further include the liquid being mounted in the incubator
Position meter, the liquid level gauge can detect the liquid level in the incubator, and the feed liquor control piece can be according to the Liquid level
The flowing velocity of liquid in the inlet tube.
5. sample fixing device according to claim 1, which is characterized in that further include be connected with the liquid outlet it is useless
Liquid bottle, the liquid in the incubator can be flowed by the liquid outlet in the waste liquid bottle.
6. sample fixing device according to claim 1, which is characterized in that further include that one end is connected with the liquid outlet
Outlet tube and go out hydraulic control product on the outlet tube, it is described go out hydraulic control product can control in the outlet tube
The flowing velocity of liquid.
7. sample fixing device according to claim 1, which is characterized in that further include being mounted on consolidating in the incubator
Determine component, the fixation kit includes fitting, and the fitting can be abutted against with the detected sample, so that described wait for
Detection sample can be clamped and fixed between the fitting and the inner wall of the incubator.
8. sample fixing device according to claim 7, which is characterized in that the incubator has bottom wall, the abutting
Part can be slided towards the direction closer or far from the incubator bottom wall, and the fitting can be with the detected sample phase
It abuts, the fitting can grip the detected sample jointly with the bottom wall of the incubator.
9. sample fixing device according to claim 8, which is characterized in that the fixation kit further include be fixed on it is described
Fixing piece in incubator, the fitting include rod-shaped mounting portion and the bar shaped that extends from the bending of one end of the mounting portion
Abutting part, the mounting portion is mounted on the fixing piece, and can be towards the side closer or far from the incubator bottom wall
To sliding, the mounting portion can also be rotated relative to the fixing piece, and the abutting part can offset with the detected sample
It connects.
10. sample fixing device according to claim 9, which is characterized in that the abutting part is equipped with arc convex, institute
Stating arc convex can abut against with the detected sample.
11. sample fixing device according to claim 1, which is characterized in that further include gas supply assembly, the gas supply assembly
It is connected with the incubator, the gas supply assembly can input carbon dioxide into the incubator.
12. a kind of nano-indenter test system, which is characterized in that include the sample as described in claim 1~11 any one
Fixing device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
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| CN118190476A (en) * | 2024-03-11 | 2024-06-14 | 优口净水科技集团(广东)有限公司 | Test bed for drinking water system |
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