GB1537086A - Immobilized enzyme conjugates - Google Patents
Immobilized enzyme conjugatesInfo
- Publication number
- GB1537086A GB1537086A GB6118/76A GB611876A GB1537086A GB 1537086 A GB1537086 A GB 1537086A GB 6118/76 A GB6118/76 A GB 6118/76A GB 611876 A GB611876 A GB 611876A GB 1537086 A GB1537086 A GB 1537086A
- Authority
- GB
- United Kingdom
- Prior art keywords
- enzyme
- support
- polymer
- base material
- functional groups
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
- C12N11/089—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
- C12N11/091—Phenol resins; Amino resins
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J31/00—Catalysts comprising hydrides, coordination complexes or organic compounds
- B01J31/02—Catalysts comprising hydrides, coordination complexes or organic compounds containing organic compounds or metal hydrides
- B01J31/0234—Nitrogen-, phosphorus-, arsenic- or antimony-containing compounds
- B01J31/0235—Nitrogen containing compounds
- B01J31/0254—Nitrogen containing compounds on mineral substrates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J31/00—Catalysts comprising hydrides, coordination complexes or organic compounds
- B01J31/003—Catalysts comprising hydrides, coordination complexes or organic compounds containing enzymes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J31/00—Catalysts comprising hydrides, coordination complexes or organic compounds
- B01J31/02—Catalysts comprising hydrides, coordination complexes or organic compounds containing organic compounds or metal hydrides
- B01J31/06—Catalysts comprising hydrides, coordination complexes or organic compounds containing organic compounds or metal hydrides containing polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
- C12N11/089—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
- C12N11/093—Polyurethanes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Materials Engineering (AREA)
- Inorganic Chemistry (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
The enzyme fixed to a support is composed of a combined organic-inorganic base material, which consists of an inorganic porous support having an organic polymer, which contains functional groups, partially adsorbed and enclosed in its pores, and of an enzyme which is partially adsorbed to the base material and bound covalently to the functional groups of the organic polymer in the terminal regions of this polymer. Such immobilised enzymes are of use in the biochemical and fermentation industries. They are obtained, in particular, by drenching a porous support with a solution of a polyfunctional organic compound and treating the impregnated support with an excess of a bifunctional monomer. Subsequently, the enzyme is added, part of which enzyme then binds covalently to reactive groups of the polymer which has been formed and which is present in and on the support. During this procedure, the biochemically active residues of the enzyme must not be blocked or altered.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US55089875A | 1975-02-18 | 1975-02-18 |
Publications (1)
Publication Number | Publication Date |
---|---|
GB1537086A true GB1537086A (en) | 1978-12-29 |
Family
ID=24199032
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB6118/76A Expired GB1537086A (en) | 1975-02-18 | 1976-02-17 | Immobilized enzyme conjugates |
Country Status (26)
Country | Link |
---|---|
JP (1) | JPS571988B2 (en) |
AR (1) | AR215240A1 (en) |
AT (1) | AT363890B (en) |
AU (1) | AU497243B2 (en) |
BE (1) | BE838684A (en) |
BR (1) | BR7600989A (en) |
CA (1) | CA1058538A (en) |
CH (1) | CH634876A5 (en) |
CS (1) | CS209424B2 (en) |
DD (1) | DD123346A5 (en) |
DE (1) | DE2605797C3 (en) |
DK (1) | DK145104C (en) |
EG (1) | EG11923A (en) |
ES (1) | ES445248A1 (en) |
FR (1) | FR2301533A1 (en) |
GB (1) | GB1537086A (en) |
IE (1) | IE42482B1 (en) |
IT (1) | IT1055978B (en) |
LU (1) | LU74379A1 (en) |
NL (1) | NL7601580A (en) |
NO (1) | NO148600C (en) |
PL (1) | PL102119B1 (en) |
PT (1) | PT64790B (en) |
SE (1) | SE434064B (en) |
YU (1) | YU37476A (en) |
ZA (1) | ZA76871B (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4335017A (en) | 1975-12-15 | 1982-06-15 | United Kingdom Atomic Energy Authority | Composite materials comprising deformable xerogel within the pores of particulate rigid supports useful in chromatography |
US9434973B2 (en) | 2009-05-20 | 2016-09-06 | Xyleco, Inc. | Bioprocessing |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2636206C3 (en) * | 1976-08-12 | 1981-06-04 | C.H. Boehringer Sohn, 6507 Ingelheim | Carrier-fixed enzymes and their manufacture and use |
CA1128917A (en) * | 1978-10-16 | 1982-08-03 | Ronald P. Rohrbach | Support matrices for immobilized enzymes |
FR2531452B1 (en) * | 1982-08-05 | 1985-06-28 | Uop Inc | MAGNETIC SUPPORT MATRIX AND IMMOBILIZED ENZYME SYSTEM HAVING APPLICATION |
US4539294A (en) * | 1982-09-30 | 1985-09-03 | Akzona Incorporated | Immobilization of proteins on polymeric supports |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3556945A (en) * | 1968-02-05 | 1971-01-19 | Corning Glass Works | Enzyme stabilization |
CH453269A4 (en) * | 1968-03-29 | 1973-01-31 | ||
GB1238280A (en) * | 1968-11-06 | 1971-07-07 | ||
US3715278A (en) * | 1970-02-11 | 1973-02-06 | Monsanto Co | Enzyme-polymer product attached to surface of siliceous materials thereof |
US3796634A (en) * | 1970-03-19 | 1974-03-12 | Us Health Education & Welfare | Insolubilized biologically active enzymes |
CH533139A (en) * | 1971-06-21 | 1973-01-31 | Nestle Sa | Process for preparing a product endowed with enzymatic activity, insoluble in aqueous medium |
US3802909A (en) * | 1971-11-09 | 1974-04-09 | American Hospital Supply Corp | Bonding of organic materials to inorganic particles |
GB1484565A (en) * | 1972-07-13 | 1977-09-01 | Koch Light Labor Ltd | Binding of biologically active macromolecules |
GB1444539A (en) * | 1972-09-11 | 1976-08-04 | Novo Industri As | Immobilised enzymes |
CH579109A5 (en) * | 1973-02-22 | 1976-08-31 | Givaudan & Cie Sa | |
SE7410542L (en) * | 1974-01-29 | 1976-01-12 | Givaudan & Cie Sa | CONDENSATION PRODUCTS. |
-
1976
- 1976-02-12 PT PT64790A patent/PT64790B/en unknown
- 1976-02-13 ZA ZA871A patent/ZA76871B/en unknown
- 1976-02-13 DE DE2605797A patent/DE2605797C3/en not_active Expired
- 1976-02-16 CH CH187576A patent/CH634876A5/en not_active IP Right Cessation
- 1976-02-17 YU YU00374/76A patent/YU37476A/en unknown
- 1976-02-17 NL NL7601580A patent/NL7601580A/en active Search and Examination
- 1976-02-17 PL PL1976187276A patent/PL102119B1/en unknown
- 1976-02-17 CS CS761011A patent/CS209424B2/en unknown
- 1976-02-17 SE SE7601757A patent/SE434064B/en not_active IP Right Cessation
- 1976-02-17 ES ES445248A patent/ES445248A1/en not_active Expired
- 1976-02-17 FR FR7604271A patent/FR2301533A1/en active Granted
- 1976-02-17 CA CA245,940A patent/CA1058538A/en not_active Expired
- 1976-02-17 DK DK63776A patent/DK145104C/en not_active IP Right Cessation
- 1976-02-17 NO NO760511A patent/NO148600C/en unknown
- 1976-02-17 AU AU11173/76A patent/AU497243B2/en not_active Expired
- 1976-02-17 BR BR7600989A patent/BR7600989A/en unknown
- 1976-02-17 GB GB6118/76A patent/GB1537086A/en not_active Expired
- 1976-02-17 DD DD191282A patent/DD123346A5/xx unknown
- 1976-02-18 LU LU74379A patent/LU74379A1/xx unknown
- 1976-02-18 EG EG101/76A patent/EG11923A/en active
- 1976-02-18 AR AR262289A patent/AR215240A1/en active
- 1976-02-18 IT IT20283/76A patent/IT1055978B/en active
- 1976-02-18 AT AT0116176A patent/AT363890B/en not_active IP Right Cessation
- 1976-02-18 IE IE317/76A patent/IE42482B1/en unknown
- 1976-02-18 BE BE164417A patent/BE838684A/en not_active IP Right Cessation
- 1976-02-18 JP JP1608176A patent/JPS571988B2/ja not_active Expired
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4335017A (en) | 1975-12-15 | 1982-06-15 | United Kingdom Atomic Energy Authority | Composite materials comprising deformable xerogel within the pores of particulate rigid supports useful in chromatography |
US4336161A (en) | 1975-12-15 | 1982-06-22 | United Kingdom Atomic Energy Authority | Composite materials comprising deformable xerogel within the pores of particulate rigid supports useful in chromatography |
US9434973B2 (en) | 2009-05-20 | 2016-09-06 | Xyleco, Inc. | Bioprocessing |
US9518281B1 (en) | 2009-05-20 | 2016-12-13 | Xyleco, Inc. | Bioprocessing |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PS | Patent sealed [section 19, patents act 1949] | ||
PCNP | Patent ceased through non-payment of renewal fee |
Effective date: 19930217 |