GB1537086A - Immobilized enzyme conjugates - Google Patents

Immobilized enzyme conjugates

Info

Publication number
GB1537086A
GB1537086A GB6118/76A GB611876A GB1537086A GB 1537086 A GB1537086 A GB 1537086A GB 6118/76 A GB6118/76 A GB 6118/76A GB 611876 A GB611876 A GB 611876A GB 1537086 A GB1537086 A GB 1537086A
Authority
GB
United Kingdom
Prior art keywords
enzyme
support
polymer
base material
functional groups
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB6118/76A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Honeywell UOP LLC
Original Assignee
UOP LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by UOP LLC filed Critical UOP LLC
Publication of GB1537086A publication Critical patent/GB1537086A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/089—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/091—Phenol resins; Amino resins
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J31/00—Catalysts comprising hydrides, coordination complexes or organic compounds
    • B01J31/02—Catalysts comprising hydrides, coordination complexes or organic compounds containing organic compounds or metal hydrides
    • B01J31/0234—Nitrogen-, phosphorus-, arsenic- or antimony-containing compounds
    • B01J31/0235—Nitrogen containing compounds
    • B01J31/0254—Nitrogen containing compounds on mineral substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J31/00—Catalysts comprising hydrides, coordination complexes or organic compounds
    • B01J31/003—Catalysts comprising hydrides, coordination complexes or organic compounds containing enzymes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J31/00—Catalysts comprising hydrides, coordination complexes or organic compounds
    • B01J31/02—Catalysts comprising hydrides, coordination complexes or organic compounds containing organic compounds or metal hydrides
    • B01J31/06—Catalysts comprising hydrides, coordination complexes or organic compounds containing organic compounds or metal hydrides containing polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/089—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/093—Polyurethanes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Materials Engineering (AREA)
  • Inorganic Chemistry (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

The enzyme fixed to a support is composed of a combined organic-inorganic base material, which consists of an inorganic porous support having an organic polymer, which contains functional groups, partially adsorbed and enclosed in its pores, and of an enzyme which is partially adsorbed to the base material and bound covalently to the functional groups of the organic polymer in the terminal regions of this polymer. Such immobilised enzymes are of use in the biochemical and fermentation industries. They are obtained, in particular, by drenching a porous support with a solution of a polyfunctional organic compound and treating the impregnated support with an excess of a bifunctional monomer. Subsequently, the enzyme is added, part of which enzyme then binds covalently to reactive groups of the polymer which has been formed and which is present in and on the support. During this procedure, the biochemically active residues of the enzyme must not be blocked or altered.
GB6118/76A 1975-02-18 1976-02-17 Immobilized enzyme conjugates Expired GB1537086A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US55089875A 1975-02-18 1975-02-18

Publications (1)

Publication Number Publication Date
GB1537086A true GB1537086A (en) 1978-12-29

Family

ID=24199032

Family Applications (1)

Application Number Title Priority Date Filing Date
GB6118/76A Expired GB1537086A (en) 1975-02-18 1976-02-17 Immobilized enzyme conjugates

Country Status (26)

Country Link
JP (1) JPS571988B2 (en)
AR (1) AR215240A1 (en)
AT (1) AT363890B (en)
AU (1) AU497243B2 (en)
BE (1) BE838684A (en)
BR (1) BR7600989A (en)
CA (1) CA1058538A (en)
CH (1) CH634876A5 (en)
CS (1) CS209424B2 (en)
DD (1) DD123346A5 (en)
DE (1) DE2605797C3 (en)
DK (1) DK145104C (en)
EG (1) EG11923A (en)
ES (1) ES445248A1 (en)
FR (1) FR2301533A1 (en)
GB (1) GB1537086A (en)
IE (1) IE42482B1 (en)
IT (1) IT1055978B (en)
LU (1) LU74379A1 (en)
NL (1) NL7601580A (en)
NO (1) NO148600C (en)
PL (1) PL102119B1 (en)
PT (1) PT64790B (en)
SE (1) SE434064B (en)
YU (1) YU37476A (en)
ZA (1) ZA76871B (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4335017A (en) 1975-12-15 1982-06-15 United Kingdom Atomic Energy Authority Composite materials comprising deformable xerogel within the pores of particulate rigid supports useful in chromatography
US9434973B2 (en) 2009-05-20 2016-09-06 Xyleco, Inc. Bioprocessing

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2636206C3 (en) * 1976-08-12 1981-06-04 C.H. Boehringer Sohn, 6507 Ingelheim Carrier-fixed enzymes and their manufacture and use
CA1128917A (en) * 1978-10-16 1982-08-03 Ronald P. Rohrbach Support matrices for immobilized enzymes
FR2531452B1 (en) * 1982-08-05 1985-06-28 Uop Inc MAGNETIC SUPPORT MATRIX AND IMMOBILIZED ENZYME SYSTEM HAVING APPLICATION
US4539294A (en) * 1982-09-30 1985-09-03 Akzona Incorporated Immobilization of proteins on polymeric supports

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3556945A (en) * 1968-02-05 1971-01-19 Corning Glass Works Enzyme stabilization
CH453269A4 (en) * 1968-03-29 1973-01-31
GB1238280A (en) * 1968-11-06 1971-07-07
US3715278A (en) * 1970-02-11 1973-02-06 Monsanto Co Enzyme-polymer product attached to surface of siliceous materials thereof
US3796634A (en) * 1970-03-19 1974-03-12 Us Health Education & Welfare Insolubilized biologically active enzymes
CH533139A (en) * 1971-06-21 1973-01-31 Nestle Sa Process for preparing a product endowed with enzymatic activity, insoluble in aqueous medium
US3802909A (en) * 1971-11-09 1974-04-09 American Hospital Supply Corp Bonding of organic materials to inorganic particles
GB1484565A (en) * 1972-07-13 1977-09-01 Koch Light Labor Ltd Binding of biologically active macromolecules
GB1444539A (en) * 1972-09-11 1976-08-04 Novo Industri As Immobilised enzymes
CH579109A5 (en) * 1973-02-22 1976-08-31 Givaudan & Cie Sa
SE7410542L (en) * 1974-01-29 1976-01-12 Givaudan & Cie Sa CONDENSATION PRODUCTS.

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4335017A (en) 1975-12-15 1982-06-15 United Kingdom Atomic Energy Authority Composite materials comprising deformable xerogel within the pores of particulate rigid supports useful in chromatography
US4336161A (en) 1975-12-15 1982-06-22 United Kingdom Atomic Energy Authority Composite materials comprising deformable xerogel within the pores of particulate rigid supports useful in chromatography
US9434973B2 (en) 2009-05-20 2016-09-06 Xyleco, Inc. Bioprocessing
US9518281B1 (en) 2009-05-20 2016-12-13 Xyleco, Inc. Bioprocessing

Also Published As

Publication number Publication date
NO148600B (en) 1983-08-01
DE2605797A1 (en) 1976-08-26
SE7601757L (en) 1976-08-19
CS209424B2 (en) 1981-12-31
IT1055978B (en) 1982-01-11
CA1058538A (en) 1979-07-17
CH634876A5 (en) 1983-02-28
SE434064B (en) 1984-07-02
JPS571988B2 (en) 1982-01-13
DK145104B (en) 1982-08-30
NO760511L (en) 1976-08-19
NL7601580A (en) 1976-08-20
DK145104C (en) 1983-02-21
YU37476A (en) 1984-02-29
AR215240A1 (en) 1979-09-28
DD123346A5 (en) 1976-12-12
DE2605797C3 (en) 1986-02-13
IE42482B1 (en) 1980-08-13
EG11923A (en) 1978-03-29
JPS51106785A (en) 1976-09-21
PT64790A (en) 1976-03-01
PL102119B1 (en) 1979-03-31
AU497243B2 (en) 1978-12-07
BE838684A (en) 1976-06-16
LU74379A1 (en) 1976-08-13
AU1117376A (en) 1977-08-25
ATA116176A (en) 1981-02-15
FR2301533B1 (en) 1978-06-23
NO148600C (en) 1983-11-09
ES445248A1 (en) 1977-10-01
DE2605797B2 (en) 1977-09-22
PT64790B (en) 1977-07-07
BR7600989A (en) 1976-09-14
DK63776A (en) 1976-08-19
FR2301533A1 (en) 1976-09-17
IE42482L (en) 1976-08-18
AT363890B (en) 1981-09-10
ZA76871B (en) 1977-01-26

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Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PCNP Patent ceased through non-payment of renewal fee

Effective date: 19930217