JPS5688796A - Enzyme immobilized film and its preparation - Google Patents
Enzyme immobilized film and its preparationInfo
- Publication number
- JPS5688796A JPS5688796A JP16504679A JP16504679A JPS5688796A JP S5688796 A JPS5688796 A JP S5688796A JP 16504679 A JP16504679 A JP 16504679A JP 16504679 A JP16504679 A JP 16504679A JP S5688796 A JPS5688796 A JP S5688796A
- Authority
- JP
- Japan
- Prior art keywords
- film
- enzyme
- immobilized
- micropores
- enzymes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
PURPOSE: To obtain an enzyme immobilized film capable of being used continuously and repeatedly, by immobilizing insolubly enzymes in the interior of the micropores and on the surface of a film having micropores.
CONSTITUTION: The carrier 1, a film having micropores, is dipped in an enzyme solution and subjected to dipping treatment. When a water-repellent film is used, the film is previously subjected to hydrophilic treatment with an alcohol, acetone, etc. The interior of the micropores and the surface of the film are covered with the enzyme solution and dried optionally, and crosslinking is carried out between the enzymes by using an enzyme immobilizing reagent, e.g., glutaraldehyde, etc., and the enzymes are insolubilized and immobilized to give an enzyme immobilized film having the enzyme layer 2 immobilized in the micropores of the film 1 and the immobilized layer 3 on the surface of the film. Consequently, a very thin enzyme immobilized film with improved mechanical strength can be made, and it is independent of the properties of a film to be used as a carrier. Much more amount of enzyme can be immobilized than the conventional methods.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP54165046A JPS5913188B2 (en) | 1979-12-18 | 1979-12-18 | Manufacturing method of enzyme immobilized membrane |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP54165046A JPS5913188B2 (en) | 1979-12-18 | 1979-12-18 | Manufacturing method of enzyme immobilized membrane |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5688796A true JPS5688796A (en) | 1981-07-18 |
JPS5913188B2 JPS5913188B2 (en) | 1984-03-28 |
Family
ID=15804800
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP54165046A Expired JPS5913188B2 (en) | 1979-12-18 | 1979-12-18 | Manufacturing method of enzyme immobilized membrane |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5913188B2 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998023734A1 (en) * | 1996-11-26 | 1998-06-04 | Gkss-Forschungszentrum Geesthacht Gmbh | Polymer membrane with enzymes localised in the membrane and process for preparing products by reactions that take place in polymer membranes |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5639788A (en) * | 1979-09-05 | 1981-04-15 | Hitachi Ltd | Preparation of immobilized enzyme |
-
1979
- 1979-12-18 JP JP54165046A patent/JPS5913188B2/en not_active Expired
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5639788A (en) * | 1979-09-05 | 1981-04-15 | Hitachi Ltd | Preparation of immobilized enzyme |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998023734A1 (en) * | 1996-11-26 | 1998-06-04 | Gkss-Forschungszentrum Geesthacht Gmbh | Polymer membrane with enzymes localised in the membrane and process for preparing products by reactions that take place in polymer membranes |
Also Published As
Publication number | Publication date |
---|---|
JPS5913188B2 (en) | 1984-03-28 |
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