GB1488469A - Process and reagent for the quantitative determination of lactate - Google Patents
Process and reagent for the quantitative determination of lactateInfo
- Publication number
- GB1488469A GB1488469A GB2765876A GB2765876A GB1488469A GB 1488469 A GB1488469 A GB 1488469A GB 2765876 A GB2765876 A GB 2765876A GB 2765876 A GB2765876 A GB 2765876A GB 1488469 A GB1488469 A GB 1488469A
- Authority
- GB
- United Kingdom
- Prior art keywords
- lactate
- glutamate
- reagent
- sample
- reaction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
- C12Q1/52—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving transaminase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
1488469 Lactate determination BOEHRINGER MANNHEIM GmbH 2 July 1976 27658/76 Heading G1B Process for the quantitative determination of L-lactate in a sample by reaction with nicotinamideadenine-dinucleotide (NAD) in the presence of lactate dehydrogenase (LDH) with the formation of reduced nicotinamide-adenine-dinucleotide (NADH) and pyruvate, reaction of the latter with glutamate in the presence of glutamate-pyruvatetransaminose (GPT) with the formation of alanine and an α-ketoglutorate in alkaline buffer solution and measurement of the NADH formed to indicate the amount of L-lactate in the sample, wherein the reaction with the glutamate is carried out in the presence of at least 0.4 mol/litre of carbonate or bicarbonate. A suitable reagent for the above process comprises LDH, GPT, NAD and glutamate and is characterized by a content of 0À40-1À2 mol/litre of a carbonate buffer of pH 9À5-10À5. The reagent may also contain sodium azide. The process may also be used to determine the L-alamine content in a sample.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB2765876A GB1488469A (en) | 1976-07-02 | 1976-07-02 | Process and reagent for the quantitative determination of lactate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB2765876A GB1488469A (en) | 1976-07-02 | 1976-07-02 | Process and reagent for the quantitative determination of lactate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1488469A true GB1488469A (en) | 1977-10-12 |
Family
ID=10263161
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB2765876A Expired GB1488469A (en) | 1976-07-02 | 1976-07-02 | Process and reagent for the quantitative determination of lactate |
Country Status (1)
| Country | Link |
|---|---|
| GB (1) | GB1488469A (en) |
-
1976
- 1976-07-02 GB GB2765876A patent/GB1488469A/en not_active Expired
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PS | Patent sealed | ||
| PE20 | Patent expired after termination of 20 years |
Effective date: 19960701 |