GB1397406A - Enzymic reagent - Google Patents
Enzymic reagentInfo
- Publication number
- GB1397406A GB1397406A GB2928671A GB2928671A GB1397406A GB 1397406 A GB1397406 A GB 1397406A GB 2928671 A GB2928671 A GB 2928671A GB 2928671 A GB2928671 A GB 2928671A GB 1397406 A GB1397406 A GB 1397406A
- Authority
- GB
- United Kingdom
- Prior art keywords
- hydroxysteroids
- buffer
- thiol
- june
- exemplified
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000003153 chemical reaction reagent Substances 0.000 title abstract 2
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 abstract 3
- 239000000872 buffer Substances 0.000 abstract 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N hydrochloric acid Substances Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 abstract 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 abstract 2
- 239000002738 chelating agent Substances 0.000 abstract 2
- 150000001875 compounds Chemical class 0.000 abstract 2
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 abstract 2
- 150000003573 thiols Chemical class 0.000 abstract 2
- CXONXVMMINSQBV-NNYOXOHSSA-N (2r,3r,4s,5r)-5-[[[[(2r,3s,4r,5r)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-hydroxyphosphoryl]oxymethyl]-2-(3-carbamothioylpyridin-1-ium-1-yl)-4-hydroxyoxolan-3-olate Chemical compound NC(=S)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)[O-])=C1 CXONXVMMINSQBV-NNYOXOHSSA-N 0.000 abstract 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 108010024636 Glutathione Proteins 0.000 abstract 1
- 102000011145 Hydroxysteroid Dehydrogenases Human genes 0.000 abstract 1
- 108010062875 Hydroxysteroid Dehydrogenases Proteins 0.000 abstract 1
- BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 abstract 1
- 229930006000 Sucrose Natural products 0.000 abstract 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 abstract 1
- 239000007983 Tris buffer Substances 0.000 abstract 1
- 239000005515 coenzyme Substances 0.000 abstract 1
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 abstract 1
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethyl mercaptane Natural products CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 abstract 1
- 239000000284 extract Substances 0.000 abstract 1
- 229960003180 glutathione Drugs 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 229950006238 nadide Drugs 0.000 abstract 1
- 230000007935 neutral effect Effects 0.000 abstract 1
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 abstract 1
- 239000002798 polar solvent Substances 0.000 abstract 1
- 239000005720 sucrose Substances 0.000 abstract 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 abstract 1
- 210000002700 urine Anatomy 0.000 abstract 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Priority Applications (8)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB2928671A GB1397406A (en) | 1971-06-22 | 1971-06-22 | Enzymic reagent |
| FR7222331A FR2143177B1 (enrdf_load_stackoverflow) | 1971-06-22 | 1972-06-21 | |
| CH928772A CH573442A5 (enrdf_load_stackoverflow) | 1971-06-22 | 1972-06-21 | |
| JP6145972A JPS5414958B1 (enrdf_load_stackoverflow) | 1971-06-22 | 1972-06-21 | |
| DE19722230320 DE2230320A1 (de) | 1971-06-22 | 1972-06-21 | Reagenz zur bestimmung von hydroxysteroiden, verfahren zu dessen herstellung und dessen verwendung |
| NL7208515A NL7208515A (enrdf_load_stackoverflow) | 1971-06-22 | 1972-06-21 | |
| NO221672A NO135490C (enrdf_load_stackoverflow) | 1971-06-22 | 1972-06-21 | |
| BE785179A BE785179A (fr) | 1971-06-22 | 1972-06-21 | Reactif enzymatique pour la determination et le dosage des hydroxysteroides |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB2928671A GB1397406A (en) | 1971-06-22 | 1971-06-22 | Enzymic reagent |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1397406A true GB1397406A (en) | 1975-06-11 |
Family
ID=10289127
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB2928671A Expired GB1397406A (en) | 1971-06-22 | 1971-06-22 | Enzymic reagent |
Country Status (8)
| Country | Link |
|---|---|
| JP (1) | JPS5414958B1 (enrdf_load_stackoverflow) |
| BE (1) | BE785179A (enrdf_load_stackoverflow) |
| CH (1) | CH573442A5 (enrdf_load_stackoverflow) |
| DE (1) | DE2230320A1 (enrdf_load_stackoverflow) |
| FR (1) | FR2143177B1 (enrdf_load_stackoverflow) |
| GB (1) | GB1397406A (enrdf_load_stackoverflow) |
| NL (1) | NL7208515A (enrdf_load_stackoverflow) |
| NO (1) | NO135490C (enrdf_load_stackoverflow) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0037742A3 (en) * | 1980-04-09 | 1982-06-02 | Nyegaard & Co. A/S | Method for the estimation of hydroxysteroids and reagent system therefor |
| US6380380B1 (en) * | 1999-01-04 | 2002-04-30 | Specialty Assays, Inc. | Use of nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucliotide phosphate (NADP) analogs to measure enzyme activities metabolites and substrates |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2649749A1 (de) * | 1976-10-29 | 1978-05-11 | Boehringer Mannheim Gmbh | Verfahren und reagens zur bestimmung von gesamtcholesterin oder gebundenem cholesterin |
| FR2419519A1 (fr) * | 1978-03-09 | 1979-10-05 | Inst Nat Sante Rech Med | Perfectionnements apportes aux procedes de dosage d'oestrogenes et ensemble pret a l'emploi pour la realisation desdits dosages |
| FR2547926B1 (fr) * | 1983-06-27 | 1986-05-23 | Biomerieux Sa | Procede de dosage des oestrogenes et des androgenes par amplification et bioluminescence |
| JPS6131097A (ja) * | 1984-07-23 | 1986-02-13 | Toyo Jozo Co Ltd | 新規な高感度酵素的測定法 |
| JPH0687777B2 (ja) * | 1986-07-08 | 1994-11-09 | 第一化学薬品株式会社 | ３α―ヒドロキシステロイドオキシダーゼ組成物及びこれを用いる３α―ヒドロキシステロイドの定量法 |
| JP2534044B2 (ja) * | 1986-11-11 | 1996-09-11 | 第一化学薬品株式会社 | ３−オキソ−５β−ステロイドの定量法及びその定量用試薬 |
-
1971
- 1971-06-22 GB GB2928671A patent/GB1397406A/en not_active Expired
-
1972
- 1972-06-21 JP JP6145972A patent/JPS5414958B1/ja active Pending
- 1972-06-21 NL NL7208515A patent/NL7208515A/xx active Search and Examination
- 1972-06-21 FR FR7222331A patent/FR2143177B1/fr not_active Expired
- 1972-06-21 NO NO221672A patent/NO135490C/no unknown
- 1972-06-21 CH CH928772A patent/CH573442A5/xx not_active IP Right Cessation
- 1972-06-21 BE BE785179A patent/BE785179A/xx not_active IP Right Cessation
- 1972-06-21 DE DE19722230320 patent/DE2230320A1/de active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0037742A3 (en) * | 1980-04-09 | 1982-06-02 | Nyegaard & Co. A/S | Method for the estimation of hydroxysteroids and reagent system therefor |
| US6380380B1 (en) * | 1999-01-04 | 2002-04-30 | Specialty Assays, Inc. | Use of nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucliotide phosphate (NADP) analogs to measure enzyme activities metabolites and substrates |
Also Published As
| Publication number | Publication date |
|---|---|
| DE2230320A1 (de) | 1973-01-11 |
| FR2143177A1 (enrdf_load_stackoverflow) | 1973-02-02 |
| NO135490C (enrdf_load_stackoverflow) | 1977-04-13 |
| NO135490B (enrdf_load_stackoverflow) | 1977-01-03 |
| NL7208515A (enrdf_load_stackoverflow) | 1972-12-28 |
| FR2143177B1 (enrdf_load_stackoverflow) | 1973-07-13 |
| BE785179A (fr) | 1972-12-21 |
| CH573442A5 (enrdf_load_stackoverflow) | 1976-03-15 |
| JPS5414958B1 (enrdf_load_stackoverflow) | 1979-06-11 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PS | Patent sealed | ||
| 732 | Registration of transactions, instruments or events in the register (sect. 32/1977) | ||
| PCNP | Patent ceased through non-payment of renewal fee |