GB1397406A - Enzymic reagent - Google Patents
Enzymic reagentInfo
- Publication number
- GB1397406A GB1397406A GB2928671A GB2928671A GB1397406A GB 1397406 A GB1397406 A GB 1397406A GB 2928671 A GB2928671 A GB 2928671A GB 2928671 A GB2928671 A GB 2928671A GB 1397406 A GB1397406 A GB 1397406A
- Authority
- GB
- United Kingdom
- Prior art keywords
- hydroxysteroids
- buffer
- thiol
- june
- exemplified
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
1397406 Determination of hydroxysteroids NYEGAARD & CO AS 19 June 1972 [22 June 1971] 29286/71 Heading G1B A reagent for determination of hydroxysteroids comprises a lyophilized mixture of a hydroxysteroid dehydrogenase, a coenzyme which is nicotinamide adenine dinucleotide or thionicotinamide adenine dinucleotide, and optionally a neutral buffer, a polyhydroxy compound, a chelating agent, and/or a thiol. The polyhydroxy compound may be a dextranor sugar (sucrose exemplified); the chelating agent may be EDTA; the thiol may be dithiothreitol (exemplified), mercaptoethanol, cysterine or glutathione. For determinations on urine, the sample is treated with conc-hydrochloric acid and formalin and the hydroxysteroids are extracted using first a non-polar then a polar solvent. The mixed extracts are evaporated to dryness and the residue dissolved in methanol for testing by mixing with the enzyme and co-enzyme. The buffer may provide pH 6-10, preferably 9-10, and may be a tris (2-amino-2-hydroxymethyl-1,3-propane diol)/hydrochloric acid buffer.
Priority Applications (8)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB2928671A GB1397406A (en) | 1971-06-22 | 1971-06-22 | Enzymic reagent |
JP6145972A JPS5414958B1 (en) | 1971-06-22 | 1972-06-21 | |
NL7208515A NL7208515A (en) | 1971-06-22 | 1972-06-21 | |
FR7222331A FR2143177B1 (en) | 1971-06-22 | 1972-06-21 | |
NO221672A NO135490C (en) | 1971-06-22 | 1972-06-21 | |
CH928772A CH573442A5 (en) | 1971-06-22 | 1972-06-21 | |
DE19722230320 DE2230320A1 (en) | 1971-06-22 | 1972-06-21 | REAGENT FOR THE DETERMINATION OF HYDROXYSTEROIDS, PROCESS FOR THEIR PRODUCTION AND THEIR USE |
BE785179A BE785179A (en) | 1971-06-22 | 1972-06-21 | ENZYMATIC REAGENT FOR DETERMINATION AND DETERMINATION OF HYDROXYSTEROIDS |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB2928671A GB1397406A (en) | 1971-06-22 | 1971-06-22 | Enzymic reagent |
Publications (1)
Publication Number | Publication Date |
---|---|
GB1397406A true GB1397406A (en) | 1975-06-11 |
Family
ID=10289127
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB2928671A Expired GB1397406A (en) | 1971-06-22 | 1971-06-22 | Enzymic reagent |
Country Status (8)
Country | Link |
---|---|
JP (1) | JPS5414958B1 (en) |
BE (1) | BE785179A (en) |
CH (1) | CH573442A5 (en) |
DE (1) | DE2230320A1 (en) |
FR (1) | FR2143177B1 (en) |
GB (1) | GB1397406A (en) |
NL (1) | NL7208515A (en) |
NO (1) | NO135490C (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0037742A2 (en) * | 1980-04-09 | 1981-10-14 | NYEGAARD & CO. A/S | Method for the estimation of hydroxysteroids and reagent system therefor |
US6380380B1 (en) * | 1999-01-04 | 2002-04-30 | Specialty Assays, Inc. | Use of nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucliotide phosphate (NADP) analogs to measure enzyme activities metabolites and substrates |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2649749A1 (en) * | 1976-10-29 | 1978-05-11 | Boehringer Mannheim Gmbh | METHOD AND REAGENT FOR THE DETERMINATION OF TOTAL CHOLESTEROL OR BONDED CHOLESTEROL |
FR2419519A1 (en) * | 1978-03-09 | 1979-10-05 | Inst Nat Sante Rech Med | IMPROVEMENTS TO THE METHODS FOR MEASURING EESTROGENS AND READY TO USE ASSEMBLY FOR THE PERFORMANCE OF THE SAID DOSES |
FR2547926B1 (en) * | 1983-06-27 | 1986-05-23 | Biomerieux Sa | ASSAY PROCESS FOR ESTROGENS AND ANDROGENS BY AMPLIFICATION AND BIOLUMINESCENCE |
JPS6131097A (en) * | 1984-07-23 | 1986-02-13 | Toyo Jozo Co Ltd | Novel enzymic method for measurement with high sensitivity |
JPH0687777B2 (en) * | 1986-07-08 | 1994-11-09 | 第一化学薬品株式会社 | 3α-hydroxysteroid oxidase composition and method for quantifying 3α-hydroxysteroid using the same |
JP2534044B2 (en) * | 1986-11-11 | 1996-09-11 | 第一化学薬品株式会社 | Method for quantifying 3-oxo-5β-steroid and reagent for quantifying the same |
-
1971
- 1971-06-22 GB GB2928671A patent/GB1397406A/en not_active Expired
-
1972
- 1972-06-21 BE BE785179A patent/BE785179A/en not_active IP Right Cessation
- 1972-06-21 NL NL7208515A patent/NL7208515A/xx active Search and Examination
- 1972-06-21 JP JP6145972A patent/JPS5414958B1/ja active Pending
- 1972-06-21 DE DE19722230320 patent/DE2230320A1/en active Pending
- 1972-06-21 NO NO221672A patent/NO135490C/no unknown
- 1972-06-21 CH CH928772A patent/CH573442A5/xx not_active IP Right Cessation
- 1972-06-21 FR FR7222331A patent/FR2143177B1/fr not_active Expired
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0037742A2 (en) * | 1980-04-09 | 1981-10-14 | NYEGAARD & CO. A/S | Method for the estimation of hydroxysteroids and reagent system therefor |
EP0037742A3 (en) * | 1980-04-09 | 1982-06-02 | Nyegaard & Co. A/S | Method for the estimation of hydroxysteroids and reagent system therefor |
US6380380B1 (en) * | 1999-01-04 | 2002-04-30 | Specialty Assays, Inc. | Use of nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucliotide phosphate (NADP) analogs to measure enzyme activities metabolites and substrates |
Also Published As
Publication number | Publication date |
---|---|
NL7208515A (en) | 1972-12-28 |
JPS5414958B1 (en) | 1979-06-11 |
BE785179A (en) | 1972-12-21 |
CH573442A5 (en) | 1976-03-15 |
FR2143177A1 (en) | 1973-02-02 |
DE2230320A1 (en) | 1973-01-11 |
NO135490B (en) | 1977-01-03 |
FR2143177B1 (en) | 1973-07-13 |
NO135490C (en) | 1977-04-13 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PS | Patent sealed | ||
732 | Registration of transactions, instruments or events in the register (sect. 32/1977) | ||
PCNP | Patent ceased through non-payment of renewal fee |