GB1397406A - Enzymic reagent - Google Patents

Enzymic reagent

Info

Publication number
GB1397406A
GB1397406A GB2928671A GB2928671A GB1397406A GB 1397406 A GB1397406 A GB 1397406A GB 2928671 A GB2928671 A GB 2928671A GB 2928671 A GB2928671 A GB 2928671A GB 1397406 A GB1397406 A GB 1397406A
Authority
GB
United Kingdom
Prior art keywords
hydroxysteroids
buffer
thiol
june
exemplified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB2928671A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nyegaard and Co AS
Original Assignee
Nyegaard and Co AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nyegaard and Co AS filed Critical Nyegaard and Co AS
Priority to GB2928671A priority Critical patent/GB1397406A/en
Priority to JP6145972A priority patent/JPS5414958B1/ja
Priority to NL7208515A priority patent/NL7208515A/xx
Priority to FR7222331A priority patent/FR2143177B1/fr
Priority to NO221672A priority patent/NO135490C/no
Priority to CH928772A priority patent/CH573442A5/xx
Priority to DE19722230320 priority patent/DE2230320A1/en
Priority to BE785179A priority patent/BE785179A/en
Publication of GB1397406A publication Critical patent/GB1397406A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

1397406 Determination of hydroxysteroids NYEGAARD & CO AS 19 June 1972 [22 June 1971] 29286/71 Heading G1B A reagent for determination of hydroxysteroids comprises a lyophilized mixture of a hydroxysteroid dehydrogenase, a coenzyme which is nicotinamide adenine dinucleotide or thionicotinamide adenine dinucleotide, and optionally a neutral buffer, a polyhydroxy compound, a chelating agent, and/or a thiol. The polyhydroxy compound may be a dextranor sugar (sucrose exemplified); the chelating agent may be EDTA; the thiol may be dithiothreitol (exemplified), mercaptoethanol, cysterine or glutathione. For determinations on urine, the sample is treated with conc-hydrochloric acid and formalin and the hydroxysteroids are extracted using first a non-polar then a polar solvent. The mixed extracts are evaporated to dryness and the residue dissolved in methanol for testing by mixing with the enzyme and co-enzyme. The buffer may provide pH 6-10, preferably 9-10, and may be a tris (2-amino-2-hydroxymethyl-1,3-propane diol)/hydrochloric acid buffer.
GB2928671A 1971-06-22 1971-06-22 Enzymic reagent Expired GB1397406A (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
GB2928671A GB1397406A (en) 1971-06-22 1971-06-22 Enzymic reagent
JP6145972A JPS5414958B1 (en) 1971-06-22 1972-06-21
NL7208515A NL7208515A (en) 1971-06-22 1972-06-21
FR7222331A FR2143177B1 (en) 1971-06-22 1972-06-21
NO221672A NO135490C (en) 1971-06-22 1972-06-21
CH928772A CH573442A5 (en) 1971-06-22 1972-06-21
DE19722230320 DE2230320A1 (en) 1971-06-22 1972-06-21 REAGENT FOR THE DETERMINATION OF HYDROXYSTEROIDS, PROCESS FOR THEIR PRODUCTION AND THEIR USE
BE785179A BE785179A (en) 1971-06-22 1972-06-21 ENZYMATIC REAGENT FOR DETERMINATION AND DETERMINATION OF HYDROXYSTEROIDS

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB2928671A GB1397406A (en) 1971-06-22 1971-06-22 Enzymic reagent

Publications (1)

Publication Number Publication Date
GB1397406A true GB1397406A (en) 1975-06-11

Family

ID=10289127

Family Applications (1)

Application Number Title Priority Date Filing Date
GB2928671A Expired GB1397406A (en) 1971-06-22 1971-06-22 Enzymic reagent

Country Status (8)

Country Link
JP (1) JPS5414958B1 (en)
BE (1) BE785179A (en)
CH (1) CH573442A5 (en)
DE (1) DE2230320A1 (en)
FR (1) FR2143177B1 (en)
GB (1) GB1397406A (en)
NL (1) NL7208515A (en)
NO (1) NO135490C (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0037742A2 (en) * 1980-04-09 1981-10-14 NYEGAARD & CO. A/S Method for the estimation of hydroxysteroids and reagent system therefor
US6380380B1 (en) * 1999-01-04 2002-04-30 Specialty Assays, Inc. Use of nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucliotide phosphate (NADP) analogs to measure enzyme activities metabolites and substrates

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2649749A1 (en) * 1976-10-29 1978-05-11 Boehringer Mannheim Gmbh METHOD AND REAGENT FOR THE DETERMINATION OF TOTAL CHOLESTEROL OR BONDED CHOLESTEROL
FR2419519A1 (en) * 1978-03-09 1979-10-05 Inst Nat Sante Rech Med IMPROVEMENTS TO THE METHODS FOR MEASURING EESTROGENS AND READY TO USE ASSEMBLY FOR THE PERFORMANCE OF THE SAID DOSES
FR2547926B1 (en) * 1983-06-27 1986-05-23 Biomerieux Sa ASSAY PROCESS FOR ESTROGENS AND ANDROGENS BY AMPLIFICATION AND BIOLUMINESCENCE
JPS6131097A (en) * 1984-07-23 1986-02-13 Toyo Jozo Co Ltd Novel enzymic method for measurement with high sensitivity
JPH0687777B2 (en) * 1986-07-08 1994-11-09 第一化学薬品株式会社 3α-hydroxysteroid oxidase composition and method for quantifying 3α-hydroxysteroid using the same
JP2534044B2 (en) * 1986-11-11 1996-09-11 第一化学薬品株式会社 Method for quantifying 3-oxo-5β-steroid and reagent for quantifying the same

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0037742A2 (en) * 1980-04-09 1981-10-14 NYEGAARD & CO. A/S Method for the estimation of hydroxysteroids and reagent system therefor
EP0037742A3 (en) * 1980-04-09 1982-06-02 Nyegaard & Co. A/S Method for the estimation of hydroxysteroids and reagent system therefor
US6380380B1 (en) * 1999-01-04 2002-04-30 Specialty Assays, Inc. Use of nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucliotide phosphate (NADP) analogs to measure enzyme activities metabolites and substrates

Also Published As

Publication number Publication date
NL7208515A (en) 1972-12-28
JPS5414958B1 (en) 1979-06-11
BE785179A (en) 1972-12-21
CH573442A5 (en) 1976-03-15
FR2143177A1 (en) 1973-02-02
DE2230320A1 (en) 1973-01-11
NO135490B (en) 1977-01-03
FR2143177B1 (en) 1973-07-13
NO135490C (en) 1977-04-13

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Legal Events

Date Code Title Description
PS Patent sealed
732 Registration of transactions, instruments or events in the register (sect. 32/1977)
PCNP Patent ceased through non-payment of renewal fee