GB1173484A - Improvements relating to microbiological methods of producing lipids. - Google Patents

Improvements relating to microbiological methods of producing lipids.

Info

Publication number
GB1173484A
GB1173484A GB1532967A GB1532967A GB1173484A GB 1173484 A GB1173484 A GB 1173484A GB 1532967 A GB1532967 A GB 1532967A GB 1532967 A GB1532967 A GB 1532967A GB 1173484 A GB1173484 A GB 1173484A
Authority
GB
United Kingdom
Prior art keywords
micro
organisms
nutrient medium
nitrogen
stage
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB1532967A
Inventor
Alexandr Zakharovich Yakovenko
Petr Nikolaevich Fisher
Ester Matveevna Dikanskaya
Alfred Nikitovich Grigorian
Bronya Moiseevna Shvartkroin
Alexandra Petrovna Krjuchkova
Alla Mikhailovna Golberg
Lidia Inanovna Komarova
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
VNII BIOSINTEZA BELKOVKH VESCH
Original Assignee
VNII BIOSINTEZA BELKOVKH VESCH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by VNII BIOSINTEZA BELKOVKH VESCH filed Critical VNII BIOSINTEZA BELKOVKH VESCH
Priority to GB1532967A priority Critical patent/GB1173484A/en
Publication of GB1173484A publication Critical patent/GB1173484A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/26—Processes using, or culture media containing, hydrocarbons
    • C12N1/28—Processes using, or culture media containing, hydrocarbons aliphatic

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

1173484 Cultivating micro-organisms on hydrocarbons VSESOJUZNY NAUCHNOISSLEDOVATELSKY INSTITUT BIOSINTEZA BELKOVYKH VESCHESTV 4 April 1967 15329/67 Index C5C 6C2 A micro-biological method of producing lipids comprises the steps of cultivating microorganisms capable of growing on a feedstock containing n-paraffin hydrocarbons on a nutrient medium containing such a feedstock, a nitrogen source, mineral compounds of phosphorus, potassium and magnesium, and trace elements, separating the micro-organisms from the nutrient medium and extracting lipids from them, and in the cultivation the nitrogen to n-paraffin hydrocarbon carbon ratio is 1:13 or lower i.e. giving more carbon relative to the nitrogen, whereby lipid production in the micro-organisms is promoted. Micro-organisms; Candida intermedia, Candida lipolytica, Candida quillermondii and Candida tropicalis, Nitrogen sources: ammonia water, sodium or potassium nitrate. The cultivation may be conducted in two stages, a first stage in which the micro-organisms are grown on a nutrient medium farowing their normal growth and protein is accumulated, and a second stage in which the nutrient medium utilized favours the production of lipids. The ratio of nitrogen to n-paraffin hydro-carbon carbon preferably is 1:13 in the first stage, but is no higher than 1:26 in the second stage. Spent nutrient medium separated from the microorganisms may be sterilized with ozone and then recycled. According to an embodiment, specified yeasts cultivated on C 11 -C 23 h-paraffins produced 18-32% of lipids based on the dry weight of the yeast. The crude lipid fraction obtained after extraction with solvent yielded 70% of polyglycerides with a molecular weight of 849 and 10% of mono- and diglycerides. The fatty acids were predominantly C 16 -C 18 . The lipid fraction also contained some hydrocarbon.
GB1532967A 1967-04-04 1967-04-04 Improvements relating to microbiological methods of producing lipids. Expired GB1173484A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB1532967A GB1173484A (en) 1967-04-04 1967-04-04 Improvements relating to microbiological methods of producing lipids.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB1532967A GB1173484A (en) 1967-04-04 1967-04-04 Improvements relating to microbiological methods of producing lipids.

Publications (1)

Publication Number Publication Date
GB1173484A true GB1173484A (en) 1969-12-10

Family

ID=10057170

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1532967A Expired GB1173484A (en) 1967-04-04 1967-04-04 Improvements relating to microbiological methods of producing lipids.

Country Status (1)

Country Link
GB (1) GB1173484A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2125195A1 (en) * 1971-02-18 1972-09-29 Inst Biosinteza Belk Microbiological petroleum distillate de waxing - with reduced nitrogen in nutrient medium to raise lipid product concn

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2125195A1 (en) * 1971-02-18 1972-09-29 Inst Biosinteza Belk Microbiological petroleum distillate de waxing - with reduced nitrogen in nutrient medium to raise lipid product concn

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Legal Events

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PS Patent sealed
PE Patent expired