SU1613492A1 - Method of producing lipids - Google Patents

Abstract

This invention relates to technical microbiology, in particular, to methods for producing lipids by microbiological synthesis. Microbial lipids can be used as substitutes for technical fats. The purpose of the invention is to increase the content of oleic acid in lipids. Strains lipogenic microscopic fungi CUNNING-HAMELLA, JAPONICA VKM F - 1204, ASPERGILLUS TERREUS VKM F-67, FUSARIUM OXYSPORUM VKM F-137 or ALTERNARIA CUCUMORINA VKM F-1881 grown deep method on a nutrient medium containing sources of carbon, nitrogen, phosphorus and incentive growth torus. The C: N ratio is 50: 1. As a carbon source, use is made of olive oil production effluent containing 3.0-3.8% of dry substances of the following composition,%: reducing substances 40-50

pectic substances 16,5-20

fats 7.5-8.0

fiber 0,2

total nitrogen 0,2-0,3

including protein 0,25-0,27

non-protein 0.01-0.015

raw protein 1,2-1,8. The RV content in wastewater is 1.5-2.5%. The biomass is then separated, dried and lipids are extracted from it. The output of lipids 30-57%. The content of oleic acid in lipids is 65.6-79.1%. 2 tab.

Description

This invention relates to technical microbiology and concerns a method for producing lipids. P1krobiologichesky synthesis. Microbial lipids can be used as substitutes for technical Zh1grav.

The purpose of the invention is to increase the content of oleic acid in lipids.

The method for producing lipids is carried out as follows.

Otammy lipigenic microscopic fungi Cunninghamella japonica VKM F-1204, Aspergillus terreus DKM F-67, Fusariun oxysporum BKM F-137

or Alternaria cucumerina BKM F-1881 is cultivated in a deep way on a nutrient medium containing the waste water from the production of olive oil (waste from oil extraction plants) as a carbon source. The medium also contains a source of nitrogen and phosphorus, preferably in the form of ammonium phosphate and a growth promoter, such as yeast autolysate.

The wastewater from the production of olive oil contains 3.0 - 3.8 dry BepiecTB (CB) of the following grams,%:

PeflyiwpyKiuifie PB40 - 50

Pectic substances 16.5 - 20 Fats 7.5 - 8.0

Cellulose0,2

Total nitrogen 0,2 - 0,3

including protein0.0, 25 - 0.27 non-protein0.01 - 0.015 Raw protein1.2-1.8 RV content in wastewater t, 5 - 2.5%. PBs consist mainly of glucose, fructose, caxapo3fji, xylose, and arabinose.

The process is conducted at a C: N ratio of 50: 1, i.e. in conditions of limiting the nitrogen content in the medium. Then the biomass is separated from the liquid phase, dried and the lipids are extracted from it.

Example 1. The strain of the microscopic fungus Cunninghamilla japonis VKM F-1204 is cultivated on a nutrient medium containing 2.5% (by PB) wastewater, 0.1% (NH) 2HP04 and 0.05% yeast autolysate. In parallel, they are grown on a medium containing 0.15% (NH4) z.MPO.

The medium is poured into 80 ml flasks with a capacity of 500 ml, adjusted to pH 6.0 and sterilized. Then, they are seeded with 7-day-old culture grown on wort agar medium. The growing process is carried out at 26 ° C for 72 hours in the deepest manner.

The resulting biomass is separated from the liquid phase, washed, dried and extracted with chloroform. From the extract, lipids are removed by vacuum.

The fatty acid composition is determined by gas chromatography on a Chromo-4 chromatograph.

The results are shown in table 1, 2.

Example 2. As a microorganism, the strain Altemia cucumerina VKM F-1881 is used. The composition of the nutrient medium is identical to example 1. C: N 50: 1.

Biomass yield 84% of PB. The output of lipids 35% of abs. dry biomass

K {ir no acid composition of lipids,% C "; o .9. C, 4: o1.6j C, b; o9.7; C, e, about 2.3, C 1ft .., 68.7, -, g 11.6, b

Example 3. The process is carried out according to gfimer 2, but as a microorganism. nizm use a strain of Aspergillus terreus BKIl F-67.

The biomass yield is 66.4% of PB, the output of pipids is 30% of abs. dry biomass.

) 1Cir but acid composition of lipids,%: C11; about With ,,; about 0,81; C (,: 0 9.5; .o 2; C ig., 71.6; C 16, 3.

Example 4. The process is carried out according to example 2, but the strain Fusarium oxysporum VKM F-137 is used as a microorganism.

Biomass yield is 66.6% of PB, lipid yield is 32% of abs. dry biomass.

Fatty acid composition of lipids,%: CH-, O 0.3; 15.6; 5.3; 0 (in .- (65.5; D 2. 2. 12.2; C d-. 1.4.

As follows from examples 1 to 4, the yield of lipids is 30 to 57%, the content of oleic acid is 65.5 to 79.1%.

Claims (1)

5 claims The method of producing lipids, including the deep cultivation of microorganisms in a nutrient medium containing carbon and nitrogen sources with a C: N ratio of 50: 1, as well as a source of phosphorus and a growth stimulator, followed by the selection of the target product, differing in that, in order to increase the content in oleic acid lipids, wastewater from the production of olive oil, containing 3.0–3.8% of dry substances of the following composition,%, is used as a carbon source: Reducing agents 40 - 50 Pectic substances 16.5 - 20 Fats 7.5 - 8.0 Fiber 0.2 nitrogen 0.2-0.3 including proteinaceous 0.25 - 0.27 non-protein 0.01 - 0.015 Sufa protein1, 2 - 1.8 and as microorganisms, the strain of lipogenic microscopic fungi Cunninghamella japonica VKM F-1204, or Aspergillus terreus VKM F-67, 55 or Fusarium oxysporum VKM F-137, or Alternaria cucumerina VKM F-1881. five 0 45 50 Table 1