GB1106887A - Sporicidal agents - Google Patents

Sporicidal agents

Info

Publication number
GB1106887A
GB1106887A GB2861966A GB2861966A GB1106887A GB 1106887 A GB1106887 A GB 1106887A GB 2861966 A GB2861966 A GB 2861966A GB 2861966 A GB2861966 A GB 2861966A GB 1106887 A GB1106887 A GB 1106887A
Authority
GB
United Kingdom
Prior art keywords
acrolein
spores
crystal
treated
spore
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB2861966A
Inventor
John Robert Norris
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shell Internationale Research Maatschappij BV
Original Assignee
Shell Internationale Research Maatschappij BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shell Internationale Research Maatschappij BV filed Critical Shell Internationale Research Maatschappij BV
Priority to GB2861966A priority Critical patent/GB1106887A/en
Priority to BE700527D priority patent/BE700527A/xx
Priority to FR111843A priority patent/FR1529169A/en
Priority to NL6708846A priority patent/NL6708846A/xx
Publication of GB1106887A publication Critical patent/GB1106887A/en
Expired legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Bacterial and fungal cultures, particularly those of insectical protein crystal-forming bacteria such as Bacillus thuringiensis, are rendered substantially free from viable spores by treating them with an olefinically unsaturated aldehyde, particularly acrolein. The culture is treated with the aldehyde at a concentration of less than 2000 ppm. at a temperature of approximately 30 DEG C. Suspensions of crystal-forming bacteria so treated may be formulated with carriers, surface active agents, wetting agents and liquid or solid diluents to give bacterial insecticide compositions in the form of dusts, granules, briquettes, wettable powders, pastes, solutions, suspensions, emulsions and emulsifiable concentrates. Examples disclose the sporicidal activity of acrolein on spores of Bacillus thuringiensis, Bacillus subtilis, Aspergillus niger, Penicillium roqueforti and Alternaria brassicicola. One comparative example demonstrates the insecticidal effect on larvae of cabbage leaves treated with (1) a spore/crystal mixture, (2) the spore/crystal mixture sterilized by treatment with acrolein for 4 hours, (3) the spore/crystal mixture autoclaved at 15 lb./sq. in. for 20 minutes and then treated with acrolein, and (4) a solution of acrolein. Mention is made of the removal of viable spores by physical means such as by dissolving out the crystal protein from the spores, removing the spores by filtration or centrifugation followed by precipitation of the protein, and treatment with irradiation, and by use of such sporicidal agents as formaldehyde, glycidaldehyde and glutaraldehyde.ALSO:Bacterial and fungal cultures, particularly those of insecticidal protein crystal-forming bacteria such as Bacillus thuringiensis, are rendered substantially free from viable spores by treating them with an olefinically unsaturated aldehyde, particularly acrolein. The culture is treated with the aldehyde at a concentration of less than 2000 ppm. at a temperature of approximately 30 DEG C. Suspensions of crystal-forming bacteria so treated may be formulated with carriers, surface active agents, wetting agents and liquid or solid diluents to give baterial insecticide compositions in the form of dusts, granules, briquettes, wettable powders, pastes, solutions, suspensions, emulsions and emulsifiable concentrates. Examples disclose the sporicidal activity of acrolein on spores of Bacillus thuringiensis, Bacillus subtilis, Aspergillus niger, Penicillium roqueforti and Alternaria brassicicola. One comparative example demonstrates the insecticidal effect on larvae of cabbage leaves treated with (1) a spore/crystal mixture, (2) the spore/crystal mixture sterilized by treatment with acrolein for 4 hours, (3) the spore/crystal mixture autoclaved at 15 lb/sq. in. for 20 minutes and then treated with acrolein, and (4) a solution of acrolein. Mention is made of the removal of viable spores by physical means such as by dissolving out the crystal protein from the spores, removing the spores by filtration or centrifugation followed by precipitation of the protein, and treatment with irradiation, and by use of such sporicidal agents as formaldehyde, glycidaldehyde and glutaraldehyde.
GB2861966A 1966-06-27 1966-06-27 Sporicidal agents Expired GB1106887A (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
GB2861966A GB1106887A (en) 1966-06-27 1966-06-27 Sporicidal agents
BE700527D BE700527A (en) 1966-06-27 1967-06-26
FR111843A FR1529169A (en) 1966-06-27 1967-06-26 Process for the production of bacterial or fungal cultures practically free from viable spores
NL6708846A NL6708846A (en) 1966-06-27 1967-06-26

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB2861966A GB1106887A (en) 1966-06-27 1966-06-27 Sporicidal agents

Publications (1)

Publication Number Publication Date
GB1106887A true GB1106887A (en) 1968-03-20

Family

ID=10278464

Family Applications (1)

Application Number Title Priority Date Filing Date
GB2861966A Expired GB1106887A (en) 1966-06-27 1966-06-27 Sporicidal agents

Country Status (3)

Country Link
BE (1) BE700527A (en)
GB (1) GB1106887A (en)
NL (1) NL6708846A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0059460A2 (en) * 1981-02-27 1982-09-08 Shionogi & Co., Ltd. An insecticide and a process for its preparation

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0059460A2 (en) * 1981-02-27 1982-09-08 Shionogi & Co., Ltd. An insecticide and a process for its preparation
EP0059460A3 (en) * 1981-02-27 1983-08-31 Shionogi & Co., Ltd. An insecticide and a process for its preparation

Also Published As

Publication number Publication date
BE700527A (en) 1967-12-27
NL6708846A (en) 1967-12-28

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