GB1077782A - Process for chemical modification of growth hormones - Google Patents
Process for chemical modification of growth hormonesInfo
- Publication number
- GB1077782A GB1077782A GB1198/65A GB119865A GB1077782A GB 1077782 A GB1077782 A GB 1077782A GB 1198/65 A GB1198/65 A GB 1198/65A GB 119865 A GB119865 A GB 119865A GB 1077782 A GB1077782 A GB 1077782A
- Authority
- GB
- United Kingdom
- Prior art keywords
- product
- modified
- solution
- recovered
- cyanogen bromide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000000122 growth hormone Substances 0.000 title abstract 3
- 238000007385 chemical modification Methods 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 abstract 2
- 238000004108 freeze drying Methods 0.000 abstract 2
- 239000000243 solution Substances 0.000 abstract 2
- 241000283690 Bos taurus Species 0.000 abstract 1
- 102000018997 Growth Hormone Human genes 0.000 abstract 1
- 108010051696 Growth Hormone Proteins 0.000 abstract 1
- QJPWUUJVYOJNMH-VKHMYHEASA-N L-homoserine lactone Chemical group N[C@H]1CCOC1=O QJPWUUJVYOJNMH-VKHMYHEASA-N 0.000 abstract 1
- 125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 abstract 1
- 229920005654 Sephadex Polymers 0.000 abstract 1
- 238000010521 absorption reaction Methods 0.000 abstract 1
- 239000002253 acid Substances 0.000 abstract 1
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 238000000502 dialysis Methods 0.000 abstract 1
- 238000010828 elution Methods 0.000 abstract 1
- 239000005556 hormone Substances 0.000 abstract 1
- 229940088597 hormone Drugs 0.000 abstract 1
- 125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 239000000843 powder Substances 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
- 239000011541 reaction mixture Substances 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/22—Hormones
- A61K38/27—Growth hormone [GH], i.e. somatotropin
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Endocrinology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Mammalian pituitary growth hormone is modified by treatment in aqueous solution with 2.5 to 10 parts by weight of cyanogen bromide at a pH of 1 to 3 and a temperature of 20 DEG to 30 DEG C. for 1/2 to 16 hours, after which the modified hormone is recovered from the reaction mixture. The completion of the reaction may be shown by estimation of increase in N-terminal amino-acid content, and of decrease in the ratio of methionine residues to homoserine lactone residues. The product may be recovered by removal of cyanogen bromide and acid added to adjust the pH, by absorption of the mixture on a cross-linked dextran gel column followed by elution of the product, preferably together with dialysis of the solution, and then subsequent freeze drying or direct lyophilisation of the solution. The product is thus isolated as an amorphous powder. Examples are given for the preparation of modified human and bovine growth hormones.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US348323A US3264186A (en) | 1964-02-26 | 1964-02-26 | Process for the purification of growth hormone |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1077782A true GB1077782A (en) | 1967-08-02 |
Family
ID=23367503
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB1198/65A Expired GB1077782A (en) | 1964-02-26 | 1965-01-11 | Process for chemical modification of growth hormones |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US3264186A (en) |
| CH (1) | CH455145A (en) |
| DE (1) | DE1620584A1 (en) |
| FR (1) | FR1584955A (en) |
| GB (1) | GB1077782A (en) |
| IL (1) | IL22728A (en) |
| NL (1) | NL6502418A (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3853833A (en) * | 1971-04-27 | 1974-12-10 | Hormone Res Foundation | Synthetic human growth-promoting and lactogenic hormones and method of producing same |
| US4765980A (en) * | 1986-04-28 | 1988-08-23 | International Minerals & Chemical Corp. | Stabilized porcine growth hormone |
-
1964
- 1964-02-26 US US348323A patent/US3264186A/en not_active Expired - Lifetime
-
1965
- 1965-01-04 IL IL6522728A patent/IL22728A/en unknown
- 1965-01-11 GB GB1198/65A patent/GB1077782A/en not_active Expired
- 1965-02-02 CH CH140165A patent/CH455145A/en unknown
- 1965-02-17 DE DE19651620584 patent/DE1620584A1/en active Pending
- 1965-02-25 FR FR1584955D patent/FR1584955A/fr not_active Expired
- 1965-02-25 NL NL6502418A patent/NL6502418A/xx unknown
Also Published As
| Publication number | Publication date |
|---|---|
| IL22728A (en) | 1968-04-25 |
| DE1620584A1 (en) | 1970-04-16 |
| FR1584955A (en) | 1970-01-09 |
| US3264186A (en) | 1966-08-02 |
| NL6502418A (en) | 1965-08-27 |
| CH455145A (en) | 1968-04-30 |
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