GB1076411A - Method of separating liquids or solutions by liquid chromatography - Google Patents

Method of separating liquids or solutions by liquid chromatography

Info

Publication number
GB1076411A
GB1076411A GB2657064A GB2657064A GB1076411A GB 1076411 A GB1076411 A GB 1076411A GB 2657064 A GB2657064 A GB 2657064A GB 2657064 A GB2657064 A GB 2657064A GB 1076411 A GB1076411 A GB 1076411A
Authority
GB
United Kingdom
Prior art keywords
tube
column
separation
wall
liquid chromatography
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB2657064A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of GB1076411A publication Critical patent/GB1076411A/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/56—Packing methods or coating methods
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6052—Construction of the column body
    • G01N30/6073—Construction of the column body in open tubular form
    • G01N30/6078—Capillaries

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)

Abstract

Liquids are separated by liquid chromatography using a separating column comprising a tube having an internal diameter between 0.05 and 2.0 millimetres and a length of at least one metre wherein the separating agent is either the inner surface of the tube itself or a liquid coating on the inner wall of the tube, or alternatively is in the form of granules which are sufficiently large in relation to the internal diameter of the tube for substantially all the granules to be supported by the wall of the tube. In the apparatus of Fig. 1 (not shown) the sample is injected through diaphragm (17) into the stream of elution liquid flowing from reservoir (10) to column (14). Examples relate to (1) separation of amino acids using a column the inner wall of which has been activated by treatment with ammonia at 300 DEG C; (2) separation of sugars using a similarly prepared column; (3) separation of terpenes using a column the inner wall of which is impregnated with polyethylene glycol (4) separation of amino acids using a copper tube the inner surface of which has been oxidised with oxygen at 180 DEG C.
GB2657064A 1963-07-03 1964-06-26 Method of separating liquids or solutions by liquid chromatography Expired GB1076411A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DEB0072516 1963-07-03

Publications (1)

Publication Number Publication Date
GB1076411A true GB1076411A (en) 1967-07-19

Family

ID=6977480

Family Applications (1)

Application Number Title Priority Date Filing Date
GB2657064A Expired GB1076411A (en) 1963-07-03 1964-06-26 Method of separating liquids or solutions by liquid chromatography

Country Status (4)

Country Link
DE (1) DE1498581A1 (en)
FR (1) FR1403251A (en)
GB (1) GB1076411A (en)
NL (1) NL6407395A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103657147A (en) * 2012-09-20 2014-03-26 中国石油化工股份有限公司 Method for separating group components of oil product

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4028056A (en) * 1976-04-20 1977-06-07 Technicon Instruments Corporation Substance separation technique
WO1988007886A1 (en) * 1987-04-15 1988-10-20 Varian Associates, Inc. Fused-silica microbore packed chromatography column with chemically modified column wall

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103657147A (en) * 2012-09-20 2014-03-26 中国石油化工股份有限公司 Method for separating group components of oil product
CN103657147B (en) * 2012-09-20 2015-08-19 中国石油化工股份有限公司 The method that oil product group composition is separated

Also Published As

Publication number Publication date
NL6407395A (en) 1965-01-04
DE1498581A1 (en) 1969-01-23
FR1403251A (en) 1965-06-18

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