GB1051309A - - Google Patents
Info
- Publication number
- GB1051309A GB1051309A GB1051309DA GB1051309A GB 1051309 A GB1051309 A GB 1051309A GB 1051309D A GB1051309D A GB 1051309DA GB 1051309 A GB1051309 A GB 1051309A
- Authority
- GB
- United Kingdom
- Prior art keywords
- tube
- naoh
- nitrogen
- pump
- heated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
- G01N31/22—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using chemical indicators
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/74—Optical detectors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/84—Preparation of the fraction to be distributed
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Peptides Or Proteins (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
1,051,309. Analysis of peptides. TECHNICON CHROMATOGRAPHY CORPORATION. Sept. 18, 1964 [Oct. 8, 1963], No. 38107/64. Heading BIX. Successive peptides eluted from a chromatography column 10 are first mixed with NaOH solution and heated to hydrolyse them and subsequently quantitatively analysed by a colorimetric technique, in a continuous process. Eluting liquid of progressively varying concentration is delivered from a multi-chamber 'variable gradient device' 12 by a piston pump 14, and the eluted stream is mixed at 52 with NaOH from tube 48 and segmentising bubbles of nitrogen from tube 50, the fluids being delivered by peristaltic pump 46. The mixture is heated in 120 feet of P.T.F.E. tubing 58 in a liquid bath 60 at 95C., to facilitate hydrolysis. A capillary tube 62 of P.V.C. continually vents some NaOH to prevent pressure build up. Nitrogen bubbles are vented at 74, and the hydrolysed peptides pass through pump 46', which may be similar to or an extension of pump 46. Acetic acid is added by tube 78 to neutralise excess NaOH; segmentising nitrogen enters the stream from tube 80. A colourdeveloping mixture of methyl cellosolve, ninhydrin and hydrindantin is added at 84 and the stream is heated in bath 88 (at 95C.), cooled in coil 90, the nitrogen bubbles are either drawn off or allowed to escape at 103 and the liquid passes through either one colorimeter cell 94, as shown, or through several cells operating at different wavelengths.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US314812A US3334969A (en) | 1963-10-08 | 1963-10-08 | Method and apparatus for chromatography analysis and hydrolysis of peptides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1051309A true GB1051309A (en) |
Family
ID=23221551
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB1051309D Active GB1051309A (en) | 1963-10-08 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US3334969A (en) |
| BE (1) | BE654083A (en) |
| CH (1) | CH469978A (en) |
| DE (1) | DE1523071B2 (en) |
| GB (1) | GB1051309A (en) |
| SE (1) | SE316929B (en) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3804593A (en) * | 1964-05-25 | 1974-04-16 | Technicon Instr | Automatic analysis apparatus and method |
| CH462501A (en) * | 1965-03-26 | 1968-09-15 | Ceskoslovenska Akademie Ved | Method and device for avoiding the remixing of individual components of a substance mixture capable of an indication reaction with an indication reagent after its separation |
| SE326056B (en) * | 1966-01-07 | 1970-07-13 | Ceskoslovenska Akademie Ved | |
| US3515491A (en) * | 1966-10-27 | 1970-06-02 | Gilford Instr Labor Inc | Fluid sample flow cell |
| US3531258A (en) * | 1967-11-16 | 1970-09-29 | Us Health Education & Welfare | Apparatus for the automated synthesis of peptides |
| US3649203A (en) * | 1968-11-22 | 1972-03-14 | Ralston Purina Co | Automatic analyzer |
| US3892532A (en) * | 1973-10-24 | 1975-07-01 | Hoffmann La Roche | Fluorescamine peptide analyzer |
| US3929413A (en) * | 1974-03-04 | 1975-12-30 | Anatronics Corp | Fluid transport and metering system |
| US4049381A (en) * | 1976-03-23 | 1977-09-20 | Technicon Instruments Corporation | Apparatus and method of fluid sample analysis |
| US4540548A (en) * | 1977-04-07 | 1985-09-10 | Yamanouchi Pharmaceutical Co., Ltd. | Method of detecting fluorescent materials and apparatus for their detection |
| US4470699A (en) * | 1982-08-12 | 1984-09-11 | The United States Of America As Represented By The United States Department Of Energy | Micro-column plasma emission liquid chromatograph |
| US4855486A (en) * | 1986-05-30 | 1989-08-08 | Kalbag Suresh M | Blocked, marked amino acids |
| US4755558A (en) * | 1986-05-30 | 1988-07-05 | Beckman Instruments, Inc. | Using internal marker |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL249032A (en) * | 1959-03-17 | 1900-01-01 |
-
0
- GB GB1051309D patent/GB1051309A/en active Active
-
1963
- 1963-10-08 US US314812A patent/US3334969A/en not_active Expired - Lifetime
-
1964
- 1964-09-30 DE DE19641523071 patent/DE1523071B2/en active Pending
- 1964-10-01 CH CH1278664A patent/CH469978A/en unknown
- 1964-10-07 SE SE12070/64A patent/SE316929B/xx unknown
- 1964-10-07 BE BE654083D patent/BE654083A/xx not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| SE316929B (en) | 1969-11-03 |
| CH469978A (en) | 1969-03-15 |
| DE1523071B2 (en) | 1971-07-29 |
| US3334969A (en) | 1967-08-08 |
| DE1523071A1 (en) | 1969-04-03 |
| BE654083A (en) | 1965-04-07 |
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