GB1027979A - Process for obtaining bcg-cultures - Google Patents

Process for obtaining bcg-cultures

Info

Publication number
GB1027979A
GB1027979A GB2597364A GB2597364A GB1027979A GB 1027979 A GB1027979 A GB 1027979A GB 2597364 A GB2597364 A GB 2597364A GB 2597364 A GB2597364 A GB 2597364A GB 1027979 A GB1027979 A GB 1027979A
Authority
GB
United Kingdom
Prior art keywords
bcg
nutrient
trade mark
registered trade
cultures
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB2597364A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF Schweiz AG
Original Assignee
Ciba AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CH873663A external-priority patent/CH465768A/en
Application filed by Ciba AG filed Critical Ciba AG
Publication of GB1027979A publication Critical patent/GB1027979A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/36—Adaptation or attenuation of cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/04—Mycobacterium, e.g. Mycobacterium tuberculosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor

Abstract

A process for obtaining BCG cultures comprises cultivating BCG under aerobic conditions in a nutrient solution containing glycerol, a source of nitrogen, inorganic salts and a wetting agent of the "TWEEN" (Registered Trade Mark) type. The aeration may be performed with 0.1 to 0.5 part by volume of air by volume of nutrient solution per minute. The BCG for cultivating in the said nutrient may be prepared by a process wherein a BCG culture is lyophilised, the lyophilisate is stored for a considerable time at the highest storage temperature of the vaccine, and then suspended in an aqueous solution, the solution plated on a solid nutrient medium in such a concentration that separate colonies grow, a few single cell colonies are propagated separately in a liquid medium, the single-cell cultures so obtained are lyophilised and in order to determine the rate of survival a few ampoules of each single-cell culture are plated on a solid medium and the single-cell cultures having the highest rate of survival are used as starting material. The nutrient medium comprises glycerol, "TWEEN" 80 (Registered Trade Mark), L-asparagine, Bacto-Casiton, potasium phosphate sodium phosphate, ferric ammonium citrate, magnesium sulphate, calcium chloride, zinc sulphate, copper sulphate and deionised water and albumin solution or sodium acetate and "Difco" (Registered Trade Mark) yeast extract.
GB2597364A 1963-07-12 1964-06-23 Process for obtaining bcg-cultures Expired GB1027979A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CH873663A CH465768A (en) 1963-07-12 1963-07-12 Process for obtaining BCG cultures
CH691364 1964-05-27

Publications (1)

Publication Number Publication Date
GB1027979A true GB1027979A (en) 1966-05-04

Family

ID=25700381

Family Applications (1)

Application Number Title Priority Date Filing Date
GB2597364A Expired GB1027979A (en) 1963-07-12 1964-06-23 Process for obtaining bcg-cultures

Country Status (8)

Country Link
BE (1) BE650404A (en)
BR (1) BR6460789D0 (en)
DE (1) DE1442075B2 (en)
DK (1) DK106687C (en)
ES (1) ES301910A1 (en)
GB (1) GB1027979A (en)
NL (1) NL6407893A (en)
SE (1) SE333432B (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0140543A2 (en) * 1983-11-03 1985-05-08 Becton Dickinson and Company Method and composition for enhancement of microorganism growth
WO2003049752A2 (en) * 2001-12-11 2003-06-19 Institut Pasteur Gram positive bacteria preparations for the treatment of diseases comprising an immune dysregulation

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0140543A2 (en) * 1983-11-03 1985-05-08 Becton Dickinson and Company Method and composition for enhancement of microorganism growth
EP0140543A3 (en) * 1983-11-03 1988-03-02 Becton, Dickinson And Company Method and composition for enhancement of microorganism growth
WO2003049752A2 (en) * 2001-12-11 2003-06-19 Institut Pasteur Gram positive bacteria preparations for the treatment of diseases comprising an immune dysregulation
WO2003049752A3 (en) * 2001-12-11 2003-11-20 Pasteur Institut Gram positive bacteria preparations for the treatment of diseases comprising an immune dysregulation
JP2005516915A (en) * 2001-12-11 2005-06-09 インスティティ・パスツール Gram-positive bacterial preparations for the treatment of diseases involving immune dysregulation
AU2002356387B2 (en) * 2001-12-11 2007-09-13 Institut National De La Sante Et De La Recherche Medicale Gram positive bacteria preparations for the treatment of diseases comprising an immune dysregulation
AU2002356387C1 (en) * 2001-12-11 2008-03-06 Institut National De La Sante Et De La Recherche Medicale Gram positive bacteria preparations for the treatment of diseases comprising an immune dysregulation
US7871627B2 (en) 2001-12-11 2011-01-18 Institut Pasteur Gram positive bacteria preparations for the treatment of disease comprising an immune dysregulation
KR101048798B1 (en) * 2001-12-11 2011-07-15 앵스티띠 나셔날 드 라 쌍뜨 에 드 라 르셰르슈 메디깔 Gram-positive bacteria preparations for the treatment of diseases including immunomodulatory disorder
CN1602199B (en) * 2001-12-11 2012-04-04 巴斯德研究院 Gram positive bacteria preparations for the treatment of diseases comprising an immune dysregulation
EP2316466A3 (en) * 2001-12-11 2012-04-11 Institut Pasteur Gram positive bacteria preparations for the treatment of diseases comprising an immune dysregulation
US8404250B2 (en) 2001-12-11 2013-03-26 Institut Pasteur Gram positive bacteria preparations for the treatment of diseases comprising an immune dysregulation

Also Published As

Publication number Publication date
NL6407893A (en) 1965-01-13
DE1442075A1 (en) 1968-11-21
ES301910A1 (en) 1965-01-01
DE1442075B2 (en) 1973-02-01
SE333432B (en) 1971-03-15
BR6460789D0 (en) 1973-07-26
DK106687C (en) 1967-03-06
BE650404A (en) 1965-01-11

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